Radiation damage to bull sperm motility. II. Proton irradiation and respiration measurements.

abstract：:Hint2, one of the five members of the superfamily of the histidine triad AMP-lysine hydrolase proteins, is expressed in mitochondria of various cell types. In human adrenocarcinoma cells, Hint2 modulates Ca(2+) handling by mitochondria. As Hint2 is highly expressed in hepatocytes, we investigated if this protein affec...

journal_title：Biophysical journal

authors： Ndiaye D,Collado-Hilly M,Martin J,Prigent S,Dufour JF,Combettes L,Dupont G

更新日期：2013-09-03 00:00:00

abstract：:Some membrane peptides, such as Alamethicin, form barrel-stave aggregates with a broad probability distribution of size (number of peptides in the aggregate). This distribution has been shown to depend on the characteristics of the lipid bilayer. A mechanism for this influence is suggested, in analogy to earlier work ...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2002-05-01 00:00:00

abstract：:The biological influence of radiation on living matter has been studied for years; however, several questions about the detailed mechanism of radiation damage formation remain largely unanswered. Among all biomolecules exposed to radiation, DNA plays an important role because any damage to its molecular structure can ...

journal_title：Biophysical journal

authors： Czapla-Masztafiak J,Szlachetko J,Milne CJ,Lipiec E,Sá J,Penfold TJ,Huthwelker T,Borca C,Abela R,Kwiatek WM

更新日期：2016-03-29 00:00:00

abstract：:Association of transmembrane (TM) helices is facilitated by the close packing of small residues present along the amino-acid sequence. Extensive studies have established the role of such small residue motifs (GxxxG) in the dimerization of Glycophorin A (GpA) and helped to elucidate the association of TM domains in the...

journal_title：Biophysical journal

authors： Prakash A,Janosi L,Doxastakis M

更新日期：2010-12-01 00:00:00

abstract：:The microenvironment between the plasma membrane and the near-membrane sarcoplasmic reticulum (SR) may play an important role in Ca(2+) regulation in smooth muscle cells. We used a three-dimensional mathematical model of Ca(2+) diffusion and regulation and experimental measurements of SR Ca(2+) uptake and the distribu...

journal_title：Biophysical journal

authors： Bazzazi H,Kargacin ME,Kargacin GJ

更新日期：2003-09-01 00:00:00

abstract：:Direct spectroscopic measurements of rotational motions of proteins and large protein segments are crucial to understanding the molecular dynamics of protein function. Fluorescent probes and spin labels attached to proteins have proved to be powerful tools in the study of large-scale protein motions. Fluorescence depo...

journal_title：Biophysical journal

authors： Thomas DD

更新日期：1978-11-01 00:00:00

abstract：:Equilibrium binding ligands usually increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. High-throughput screening for the discovery of drug-like compounds uses an assay based on thermal stabilization. The mathematical description of this stabilization is well d...

journal_title：Biophysical journal

authors： Cimmperman P,Baranauskiene L,Jachimoviciūte S,Jachno J,Torresan J,Michailoviene V,Matuliene J,Sereikaite J,Bumelis V,Matulis D

更新日期：2008-10-01 00:00:00

abstract：:Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar...

journal_title：Biophysical journal

authors： Anderson EC,Petersen DF,Tobey RA

更新日期：1970-07-01 00:00:00

abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

journal_title：Biophysical journal

authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

更新日期：1993-09-01 00:00:00

abstract：:Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

journal_title：Biophysical journal

authors： Grossman SH

更新日期：1991-03-01 00:00:00

abstract：:Small-angle neutron scattering (SANS) is used to probe the solution structure of two protein therapeutics (monoclonal antibodies 1 and 2 (MAb1 and MAb2)) and their protein-protein interaction (PPI) at high concentrations. These MAbs differ by small sequence alterations in the complementarity-determining region but sho...

journal_title：Biophysical journal

authors： Yearley EJ,Zarraga IE,Shire SJ,Scherer TM,Gokarn Y,Wagner NJ,Liu Y

更新日期：2013-08-06 00:00:00

abstract：:In biological applications of atomic force microscopy, the different surface properties of the biological sample and its support become apparent. Observed height differences between the biomolecule and its supporting surface are thus not only of structural origin, but also depend on the different sample-tip and suppor...

journal_title：Biophysical journal

authors： Müller DJ,Engel A

更新日期：1997-09-01 00:00:00

abstract：:We study a differential equation that models nerve impulse transmission. The nonlinearity is simplified to be piecewise linear in order to allow explicit solution. In general, two solitary impulse solutions are exhibited. The temporal stability of these solutions is analyzed by a technique that identifies the number o...

journal_title：Biophysical journal

authors： Feroe JA

更新日期：1978-02-01 00:00:00

abstract：:Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport; however, its precise mechanistic role ...

journal_title：Biophysical journal

authors： Crosby KC,Postma M,Hink MA,Zeelenberg CH,Adjobo-Hermans MJ,Gadella TW

更新日期：2013-05-07 00:00:00

abstract：:The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that FR-...

journal_title：Biophysical journal

authors： Chen H,Yang J,Low PS,Cheng JX

更新日期：2008-02-15 00:00:00

abstract：:The chemical unfolding transition of a protein was simulated, including the presence of an intermediate (I) in equilibrium with the native (N) and unfolded (U) states. The calculations included free energies of unfolding, DeltaGuw, in the range of 1.4 kcal/mol to 10 kcal/mol and three different global m-values. The si...

journal_title：Biophysical journal

authors： Soulages JL

更新日期：1998-07-01 00:00:00

abstract：:The unfolding and folding of protein barnase has been extensively investigated in bulk conditions under the effect of denaturant and temperature. These experiments provided information about structural and kinetic features of both the native and the unfolded states of the protein, and debates about the possible existe...

journal_title：Biophysical journal

authors： Alemany A,Rey-Serra B,Frutos S,Cecconi C,Ritort F

更新日期：2016-01-05 00:00:00

abstract：:Eukaryotic genomes are hierarchically organized into protein-DNA assemblies for compaction into the nucleus. Nucleosomes, with the (H3-H4)2 tetrasome as a likely intermediate, are highly dynamic in nature by way of several different mechanisms. We have recently shown that tetrasomes spontaneously change the direction ...

journal_title：Biophysical journal

authors： Ordu O,Lusser A,Dekker NH

更新日期：2019-12-03 00:00:00

abstract：:Sedimentation velocity is a powerful tool for the analysis of complex solutions of macromolecules. However, sample turbidity imposes an upper limit to the size of molecular complexes currently amenable to such analysis. Furthermore, the breadth of the particle size distribution, combined with possible variations in th...

journal_title：Biophysical journal

authors： Mächtle W

更新日期：1999-02-01 00:00:00

abstract：:Antibodies are key tools in biomedical research and medicine. Their binding properties are classically measured in solution and characterized by an affinity. However, in physiological conditions, antibodies can bridge an immune effector cell and an antigen-presenting cell, implying that mechanical forces may apply to ...

journal_title：Biophysical journal

authors： González C,Chames P,Kerfelec B,Baty D,Robert P,Limozin L

更新日期：2019-04-23 00:00:00

abstract：:We have explored the electromechanical properties of DNA on a nanometer-length scale using an electric field to force single molecules through synthetic nanopores in ultrathin silicon nitride membranes. At low electric fields, E < 200 mV/10 nm, we observed that single-stranded DNA can permeate pores with a diameter >/...

journal_title：Biophysical journal

authors： Heng JB,Aksimentiev A,Ho C,Marks P,Grinkova YV,Sligar S,Schulten K,Timp G

更新日期：2006-02-01 00:00:00

abstract：:The thermal transitions of fibrillar collagen are investigated with second-harmonic generation polarization anisotropy microscopy. Second-harmonic generation images and polarization anisotropy profiles of corneal stroma heated in the 35-80°C range are analyzed by means of a theoretical model that is suitable to probe ...

journal_title：Biophysical journal

authors： Matteini P,Cicchi R,Ratto F,Kapsokalyvas D,Rossi F,de Angelis M,Pavone FS,Pini R

更新日期：2012-09-19 00:00:00

abstract：:Allosteric regulation of protein function is recognized to be widespread throughout biology; however, knowledge of allosteric mechanisms, the molecular changes within a protein that couple one binding site to another, is limited. Although mutagenesis is often used to probe allosteric mechanisms, we consider herein wha...

journal_title：Biophysical journal

authors： Carlson GM,Fenton AW

更新日期：2016-05-10 00:00:00

abstract：:The engulfment function of macrophages relies on complex molecular interactions involving both lipids and proteins. In particular, the clearance of apoptotic bodies (efferocytosis) is enabled by externalization on the cell target of phosphatidylserine lipids, which activate receptors on macrophages, suggesting that (l...

journal_title：Biophysical journal

authors： Justice MJ,Petrusca DN,Rogozea AL,Williams JA,Schweitzer KS,Petrache I,Wassall SR,Petrache HI

更新日期：2014-02-04 00:00:00

abstract：:The interaction of DNA with the polyamine spermine(4+) (Spm(4+)), sodium ions, and water molecules has been studied using molecular dynamics computer simulations in a system modeling a DNA crystal. The simulation model consisted of three B-DNA decamers in a periodic hexagonal cell, containing 1200 water molecules, 8 S...

journal_title：Biophysical journal

authors： Korolev N,Lyubartsev AP,Laaksonen A,Nordenskiöld L

更新日期：2002-06-01 00:00:00

abstract：:Cells within fibrocartilaginous tissues, including chondrocytes and fibroblasts of the meniscus, ligament, and tendon, regulate cell biosynthesis in response to local mechanical stimuli. The processes by which an applied mechanical load is transferred through the extracellular matrix to the environment of a cell are n...

journal_title：Biophysical journal

authors： Upton ML,Gilchrist CL,Guilak F,Setton LA

更新日期：2008-08-01 00:00:00

abstract：:Detection algorithms are widely used for the analysis of single-channel data because they remove the background noise from the measured current signal and reconstruct the noise-free time series. Standard detection algorithms assume channels switching only between zero and full conductance. Many types of channels, howe...

journal_title：Biophysical journal

authors： Draber S,Schultze R

更新日期：1994-10-01 00:00:00

abstract：:The vibrational Raman spectra of both pure 1-alpha-dimyristoylphosphatidic acid (DMPA) liposomes and DMPA multilayers reconstituted with ferricytochrome c at pH 7 and pH 4, with either sodium or calcium as the cation, are reported as a function of temperature. Multilayers composed of a 1:1 mol ratio DMPA and dimyristo...

journal_title：Biophysical journal

authors： Vincent JS,Levin IW

更新日期：1991-05-01 00:00:00

abstract：:The surprising observation that pressures of the order of 150 atmospheres can restore consciousness to an anesthetized animal has long been central to theories of the molecular mechanisms underlying general anesthesia. We have constructed a high-pressure gas chamber to test for "pressure reversal" of the best availabl...

journal_title：Biophysical journal

authors： Moss GW,Lieb WR,Franks NP

更新日期：1991-12-01 00:00:00

abstract：:We study the lateral headgroup interactions among phosphatidylcholine (PC) molecules and among phosphatidylethanolamine (PE) molecules in monolayers and extend our previous models. In this paper, we present an extensive set of pressure-area isotherms and surface potential experiments on monolayers of phospholipids ran...

journal_title：Biophysical journal

authors： Mingins J,Stigter D,Dill KA

更新日期：1992-06-01 00:00:00