ADP compartmentation analysis reveals coupling between pyruvate kinase and ATPases in heart muscle.

abstract：:We present a theory for proton diffusion through an immobilized protein membrane perfused with an electrolyte and a buffer. Using a Nernst-Planck equation for each species and assuming local charge neutrality, we obtain two coupled nonlinear diffusion equations with new diffusion coefficients dependent on the concentr...

journal_title：Biophysical journal

authors： Zabusky NJ,Deem GS

更新日期：1979-01-01 00:00:00

abstract：:We report detection and quantification of ultraviolet (UV) damage in DNA at a single molecule level by atomic force microscopy (AFM). By combining the supercoiled plasmid relaxation assay with AFM imaging, we find that high doses of medium wave ultraviolet (UVB) and short wave ultraviolet (UVC) light not only produce ...

journal_title：Biophysical journal

authors： Jiang Y,Ke C,Mieczkowski PA,Marszalek PE

更新日期：2007-09-01 00:00:00

abstract：:Intramembrane charge movement (Q), Ca(2+) conductance (G(m)) through the dihydropyridine-sensitive L-type Ca(2+) channel (DHPR) and intracellular Ca(2+) fluorescence (F) have been recorded simultaneously in flexor digitorum brevis muscle fibers of adult mice, using the whole-cell configuration of the patch-clamp techn...

journal_title：Biophysical journal

authors： Wang ZM,Messi ML,Delbono O

更新日期：1999-11-01 00:00:00

abstract：:We investigate the microsecond-timescale kinetics of the RNA hairpin ga*cUUCGguc. The fluorescent nucleotide 2-aminopurine (a*) reports mainly on base stacking. Ten kinetic traces and the temperature denaturation curve are globally fitted to four-state models of the free-energy surface. In the best-fitting sequential ...

journal_title：Biophysical journal

authors： Sarkar K,Meister K,Sethi A,Gruebele M

更新日期：2009-09-02 00:00:00

abstract：:Fluo-3 is an unusual tetracarboxylate Ca2+ indicator. For recent lots supplied by Molecular Probes Inc. (Eugene, OR), FMAX, the fluorescence intensity of the indicator in its Ca(2+)-bound form, is approximately 200 times that of FMIN, the fluorescence intensity of the indicator in its Ca(2+)-free form. (For earlier lo...

journal_title：Biophysical journal

authors： Harkins AB,Kurebayashi N,Baylor SM

更新日期：1993-08-01 00:00:00

abstract：:A two-dimensional stochastic model for the dynamics of microtubules in gliding-assay experiments is presented here, which includes the viscous drag acting on the moving fiber and the interaction with the kinesins. For this purpose, we model kinesin as a spring, and explicitly use parameter values to characterize the m...

journal_title：Biophysical journal

authors： Gibbons F,Chauwin JF,Despósito M,José JV

更新日期：2001-06-01 00:00:00

abstract：:The coat protein of tobacco mosaic virus is known to form three different classes of aggregate, depending on environmental conditions, namely helical, disk, and A-protein. Among the disk aggregates, there are four-layer, six-layer, and long stacks, which can be obtained by varying the ionic strength and temperature co...

journal_title：Biophysical journal

authors： Díaz-Avalos R,Caspar DL

更新日期：1998-01-01 00:00:00

abstract：:A continuum model is provided of the free energy terms that contribute to the molecular association of ricin A-chain (RTA) with the rRNA substrate and several small ligands. The model for RTA interactions with the RNA was taken from a previously proposed complex containing a 29-mer oligonucleotide hairpin (. Proteins ...

journal_title：Biophysical journal

authors： Olson MA,Cuff L

更新日期：1999-01-01 00:00:00

abstract：:Sedimentation velocity is a powerful tool for the analysis of complex solutions of macromolecules. However, sample turbidity imposes an upper limit to the size of molecular complexes currently amenable to such analysis. Furthermore, the breadth of the particle size distribution, combined with possible variations in th...

journal_title：Biophysical journal

authors： Mächtle W

更新日期：1999-02-01 00:00:00

abstract：:A novel system for measuring, simultaneously, ciliary beating and intracellular free calcium is presented. The advantages and dynamic nature of the system are demonstrated by measuring the effects of the calcium ionophore lonomycin and of extracellular ATP on ciliated rabbit trachea. The results are discussed with reg...

journal_title：Biophysical journal

authors： Korngreen A,Priel Z

更新日期：1994-07-01 00:00:00

abstract：:Aromatic monocarboxylic acids are known to significantly potentiate the mechanical response of skeletal muscle fibers. In this study we investigated the effects of enantiomers of 2-(4-chlorophenoxy)propionic acid, chemically one of the simplest aromatic monocarboxylic acids with chiral properties, on mechanical thresh...

journal_title：Biophysical journal

authors： Heiny JA,Jong D,Bryant SH,Conte-Camerino D,Tortorella V

更新日期：1990-01-01 00:00:00

abstract：:One theory of freezing damage suggests that slowly cooled cells are killed by being exposed to increasing concentrations of electrolytes as the suspending medium freezes. A corollary to this view is that protective additives such as glycerol protect cells by acting colligatively to reduce the electrolyte concentration...

journal_title：Biophysical journal

authors： Rall WF,Mazur P,Souzu H

更新日期：1978-07-01 00:00:00

abstract：:The outer membrane of Gram-negative bacteria is of great scientific interest because it mediates the action of antimicrobial agents. The membrane surface is composed of lipopolysaccharide (LPS) molecules with negatively charged oligosaccharide headgroups. To a certain fraction, LPSs additionally display linear polysac...

journal_title：Biophysical journal

authors： Micciulla S,Gerelli Y,Schneck E

更新日期：2019-04-02 00:00:00

abstract：:To probe the complexity of the cell membrane organization and dynamics, it is important to obtain simple physical observables from experiments on live cells. Here we show that fluorescence correlation spectroscopy (FCS) measurements at different spatial scales enable distinguishing between different submicron confinem...

journal_title：Biophysical journal

authors： Wawrezinieck L,Rigneault H,Marguet D,Lenne PF

更新日期：2005-12-01 00:00:00

abstract：:The theoretical relationships between the fluorescence and photochemical yields of PS II and the fraction of open reaction centers are examined in a general model endowed with the following features: i) a homogeneous, infinite PS II domain; ii) exciton-radical-pair equilibrium; and iii) different rates of exciton tran...

journal_title：Biophysical journal

authors： Lavergne J,Trissl HW

更新日期：1995-06-01 00:00:00

abstract：:We present the results of a detailed molecular dynamics study of the closed form of the P2X4 receptor. The fluctuations observed in the simulations were compared with the changes that occur in the transition from the closed to the open structure. To get further insight on the opening mechanism, the actual displacement...

journal_title：Biophysical journal

authors： Pierdominici-Sottile G,Moffatt L,Palma J

更新日期：2016-12-20 00:00:00

abstract：:The amyloid fibrillar form of the protein Tau is involved in a number of neurodegenerative diseases, also known as tauopathies. In this work, six different fibrillar Tau isoforms were assembled in vitro. The morphological and nanomechanical properties of these isoforms were studied using atomic force microscopy at hig...

journal_title：Biophysical journal

authors： Makky A,Bousset L,Madiona K,Melki R

更新日期：2020-12-15 00:00:00

abstract：:The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive i...

journal_title：Biophysical journal

authors： TAYLOR EW,CRAMER W

更新日期：1963-03-01 00:00:00

abstract：:Cardiac contraction and relaxation dynamics result from a set of simultaneously interacting Ca(2+) regulatory mechanisms. In this study, cardiocyte Ca(2+) dynamics were modeled using a set of six differential equations that were based on theories, equations, and parameters described in previous studies. Among the uniq...

journal_title：Biophysical journal

authors： Snyder SM,Palmer BM,Moore RL

更新日期：2000-07-01 00:00:00

abstract：:High-bandwidth measurements of the ion current through hafnium oxide and silicon nitride nanopores allow the analysis of sub-30 kD protein molecules with unprecedented time resolution and detection efficiency. Measured capture rates suggest that at moderate transmembrane bias values, a substantial fraction of protein ...

journal_title：Biophysical journal

authors： Larkin J,Henley RY,Muthukumar M,Rosenstein JK,Wanunu M

更新日期：2014-02-04 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spec...

journal_title：Biophysical journal

authors： Apte DV,Koutalos Y,McFarlane DK,Dawson MJ,Ebrey TG

更新日期：1989-09-01 00:00:00

abstract：:The current rapid expansion of biological knowledge offers a great opportunity to rationally engineer biological systems that respond to signals such as light and chemical inducers by producing specific proteins. Turning on and off the production of proteins on demand holds great promise for creating significant biote...

journal_title：Biophysical journal

authors： Tuttle LM,Salis H,Tomshine J,Kaznessis YN

更新日期：2005-12-01 00:00:00

abstract：:We present elastic and inelastic incoherent neutron scattering data from a series of trehalose glasses diluted with glycerol. A strong correlation with recently published protein stability data in the same series of glasses illustrates that the dynamics at Q >or= 0.71 A(-1) and omega > 200 MHz are important to stabili...

journal_title：Biophysical journal

authors： Cicerone MT,Soles CL

更新日期：2004-06-01 00:00:00

abstract：:A dynamic model of smooth muscle contraction is presented and is compared with the mechanical properties of vascular smooth muscle in the rat portal vein. The model is based on the sliding filament theory and the assumption that force is produced by cross-bridges extending from the myosin to the actin filaments. Thus,...

journal_title：Biophysical journal

authors： Gestrelius S,Borgström P

更新日期：1986-07-01 00:00:00

abstract：:We present MEMLET (MATLAB-enabled maximum-likelihood estimation tool), a simple-to-use and powerful program for utilizing maximum-likelihood estimation (MLE) for parameter estimation from data produced by single-molecule and other biophysical experiments. The program is written in MATLAB and includes a graphical user ...

journal_title：Biophysical journal

authors： Woody MS,Lewis JH,Greenberg MJ,Goldman YE,Ostap EM

更新日期：2016-07-26 00:00:00

abstract：:hbAP0 is a model membrane protein designed to possess an anesthetic-binding cavity in its hydrophilic domain and a cation channel in its hydrophobic domain. Grazing incidence x-ray diffraction shows that hbAP0 forms four-helix bundles that are vectorially oriented within Langmuir monolayers at the air-water interface....

journal_title：Biophysical journal

authors： Churbanova IY,Tronin A,Strzalka J,Gog T,Kuzmenko I,Johansson JS,Blasie JK

更新日期：2006-05-01 00:00:00

abstract：:Functional RNAs can fold into intricate structures using a number of different secondary and tertiary structural motifs. Many factors contribute to the overall free energy of the target fold. This study aims at quantifying the entropic costs coming from the loss of conformational freedom when the sugar-phosphate backb...

journal_title：Biophysical journal

authors： Mak CH,Phan ENH

更新日期：2018-05-08 00:00:00

abstract：:Reconstruction of genome-scale metabolic networks is now possible using multiple different data types. Constraint-based modeling is an approach to interrogate capabilities of reconstructed networks by constraining possible cellular behavior through the imposition of physicochemical laws. As a result, a steady-state fl...

journal_title：Biophysical journal

authors： Price ND,Schellenberger J,Palsson BO

更新日期：2004-10-01 00:00:00

abstract：:The blood stage malaria parasite, the merozoite, has a small window of opportunity during which it must successfully target and invade a human erythrocyte. The process of invasion is nonetheless remarkably rapid. To date, mechanistic models of invasion have focused predominantly on the parasite actomyosin motor contri...

journal_title：Biophysical journal

authors： Dasgupta S,Auth T,Gov NS,Satchwell TJ,Hanssen E,Zuccala ES,Riglar DT,Toye AM,Betz T,Baum J,Gompper G

更新日期：2014-07-01 00:00:00

abstract：:Structures of the blood clotting enzyme thrombin complexed with hirugen and two active site inhibitors, RWJ-50353 10080(N-methyl-D-phenylalanyl-N-[5-[(aminoiminomethyl)amino]-1- [[(2-benzothiazolyl)carbonyl]butyl]-L-prolinamide trifluoroacetate hydrate) and RWJ-50215 (N-[4-(aminoiminomethyl)amino-1-[2- (thiazol-2-ylca...

journal_title：Biophysical journal

authors： Matthews JH,Krishnan R,Costanzo MJ,Maryanoff BE,Tulinsky A

更新日期：1996-11-01 00:00:00