A dynamical model of kinesin-microtubule motility assays.

abstract：:In isolated thick filaments from many types of muscle, the two head domains of each myosin molecule are folded back against the filament backbone in a conformation called the interacting heads motif (IHM) in which actin interaction is inhibited. This conformation is present in resting skeletal muscle, but it is not kn...

journal_title：Biophysical journal

authors： Fusi L,Huang Z,Irving M

更新日期：2015-08-18 00:00:00

abstract：:The M2 protein from the influenza A virus forms a proton channel in the virion that is essential for infection. This tetrameric protein appears to form a four-helix bundle spanning the viral membrane. Here the solid-state NMR method, 2D polarization inversion spin exchange at magic angle (PISEMA), has been used to obt...

journal_title：Biophysical journal

authors： Song Z,Kovacs FA,Wang J,Denny JK,Shekar SC,Quine JR,Cross TA

更新日期：2000-08-01 00:00:00

abstract：:The human brain may be regarded as an irreversible system which is constrained by a fixed inflow of free energy in the form of chemical nourishment from within the body and information from the environment. The evolution of the internal spontaneous process may be described as a path on the saddle surface of entropy pr...

journal_title：Biophysical journal

authors： Kirkaldy JS

更新日期：1965-11-01 00:00:00

abstract：:Several investigators have demonstrated that under conditions where little or no gas exchange occurs across the alveolar capillary membrane the PCO(2) is higher in the alveolus than in the mixed venous blood, whereas there are no PO(2) differences. Gurtner et al. have explained the DeltaPCO(2) by a model in which H(+)...

journal_title：Biophysical journal

authors： Gurtner GH

更新日期：1972-06-01 00:00:00

abstract：:Closing linear poly(ethylene glycol) (PEG) into a circular "crown" dramatically changes its dynamics in the alpha-hemolysin channel. In the electrically neutral crown ether (C2H4O)6, six ethylene oxide monomers are linked into a circle that gives the molecule ion-complexing capacity and increases its rigidity. As with...

journal_title：Biophysical journal

authors： Bezrukov SM,Krasilnikov OV,Yuldasheva LN,Berezhkovskii AM,Rodrigues CG

更新日期：2004-11-01 00:00:00

abstract：:We have previously observed that the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) solubilizes lipid membranes in a pH-dependent manner, and we have exploited this phenomenon to prepare lipid vesicles that release their contents in response to pH, light, or glucose (Thomas, J. L., and D. A. Tirrell. Acc. Chem....

journal_title：Biophysical journal

authors： Thomas JL,Barton SW,Tirrell DA

更新日期：1994-09-01 00:00:00

abstract：:Quantum chemical computations using the ab initio molecular orbital (MO) method have been performed to investigate the molecular switching mechanism of Ras protein p21, which has an important role in intracellular signal cascades. Lys(16) was demonstrated to be crucial to the function of Ras p21, and the hydrolysis of...

journal_title：Biophysical journal

authors： Futatsugi N,Hata M,Hoshino T,Tsuda M

更新日期：1999-12-01 00:00:00

abstract：:The muscle nicotinic receptor (AChR) is a pentamer of four different subunits, each of which contains four transmembrane domains (M1-M4). We recently showed that channel opening and closing rates of the AChR depend on a hydrogen bond involving a threonine at position 14' of the M4 domain in the alpha-subunit. To deter...

journal_title：Biophysical journal

authors： Bouzat C,Gumilar F,del Carmen Esandi M,Sine SM

更新日期：2002-04-01 00:00:00

abstract：:Under the assumption that vesicles are the anatomic correlate of quantal release, the forces governing the movement of synaptic vesicles inside neurons are analyzed. Semiquantitative calculations are presented to show that a diffuse layer field penetrates a few Debye lengths into the axoplasm. This field binds tightly...

journal_title：Biophysical journal

authors： Remler MP

更新日期：1973-02-01 00:00:00

abstract：:Single-molecule imaging analysis of chemotactic response in eukaryotic cells has revealed a stochastic nature in the input signals and the signal transduction processes. This leads to a fundamental question about the signaling processes: how does the signaling system operate under stochastic fluctuations or noise? Her...

journal_title：Biophysical journal

authors： Ueda M,Shibata T

更新日期：2007-07-01 00:00:00

abstract：:The dynamic packaging of DNA into chromatin is a key determinant of eukaryotic gene regulation and epigenetic inheritance. Nucleosomes are the basic unit of chromatin, and therefore the accessible states of the nucleosome must be the starting point for mechanistic models regarding these essential processes. Although t...

journal_title：Biophysical journal

authors： Schlingman DJ,Mack AH,Kamenetska M,Mochrie SGJ,Regan L

更新日期：2014-07-15 00:00:00

abstract：:Myoglobin (Mb) is a model system for ligand binding and migration. The energy barriers (ΔG) for ligand migration in Mb have been studied in the past by experiment and theory and significant differences between different approaches were found. From experiment, it is known that Mb can assume a large number of conformati...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2012-01-18 00:00:00

abstract：:Conduction speed (theta) in single myelinated Rana pipiens sciatic nerve fibers has been precisely measured using intracellular recording and on-line digital computer techniques. The dependence of relative speed on external Na concentration at 15 degrees C has been found to be ln(theta(1)/theta(2)) = 0.524 (+/-0.018) ...

journal_title：Biophysical journal

authors： Hardy WL

更新日期：1973-10-01 00:00:00

abstract：:We study the stochastic dynamics of growth and shrinkage of single actin filaments taking into account insertion, removal, and ATP hydrolysis of subunits either according to the vectorial mechanism or to the random mechanism. In a previous work, we developed a model for a single actin or microtubule filament where hyd...

journal_title：Biophysical journal

authors： Ranjith P,Mallick K,Joanny JF,Lacoste D

更新日期：2010-04-21 00:00:00

abstract：:The structure and biophysical properties of lipid membranes are important for cellular functions in health and disease. In Alzheimer's disease, the neuronal membrane is a target for toxic amyloid-β (Aβ). Melatonin is an important pineal gland hormone that has been shown to protect against Aβ toxicity in cellular and a...

journal_title：Biophysical journal

authors： Mei N,Robinson M,Davis JH,Leonenko Z

更新日期：2020-12-15 00:00:00

abstract：:To explain the effect of profilin on actin critical concentration in a manner consistent with thermodynamic constraints and available experimental data, we built a thermodynamically rigorous model of actin steady-state dynamics in the presence of profilin. We analyzed previously published mechanisms theoretically and ...

journal_title：Biophysical journal

authors： Yarmola EG,Dranishnikov DA,Bubb MR

更新日期：2008-12-15 00:00:00

abstract：:Cyclic-nucleotide-activated, nonselective cation channels have a central role in sensory transduction. They are most likely tetramers, composed of two subunits (alpha and beta or 1 and 2), with the former, but not the latter, being able to form homomeric cyclic-nucleotide-activated channels. Identified members of this...

journal_title：Biophysical journal

authors： Finn JT,Krautwurst D,Schroeder JE,Chen TY,Reed RR,Yau KW

更新日期：1998-03-01 00:00:00

abstract：:We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring pe...

journal_title：Biophysical journal

authors： Kelly CV,Baird BA,Craighead HG

更新日期：2011-04-06 00:00:00

abstract：:We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intri...

journal_title：Biophysical journal

authors： Li Q,Wu SS,Chou KC

更新日期：2009-12-16 00:00:00

abstract：:We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Phosphatase exclusion generates regions of low phosphatase and high kinase activity and ...

journal_title：Biophysical journal

authors： Burroughs NJ,Lazic Z,van der Merwe PA

更新日期：2006-09-01 00:00:00

abstract：:Segmentation of the Drosophila melanogaster embryo results from the dynamic establishment of spatial mRNA and protein patterns. Here, we exploit recent temporal mRNA and protein expression measurements on the full surface of the blastoderm to calibrate a dynamical model of the gap gene network on the entire embryo cor...

journal_title：Biophysical journal

authors： Bieler J,Pozzorini C,Naef F

更新日期：2011-07-20 00:00:00

abstract：:During protein synthesis dictated by the codon sequence of messenger RNA, the ribosome selects aminoacyl-tRNA (aa-tRNA) with high accuracy, the exact mechanism of which remains elusive. By using a single-molecule fluorescence resonance energy transfer method coupled with fluorescence emission anisotropy, we provide ev...

journal_title：Biophysical journal

authors： Mishra PP,Qureshi MT,Ren W,Lee TH

更新日期：2010-12-01 00:00:00

abstract：:The interactions between phospholipid molecules in suspensions have been studied by using mass spectrometry. Electrospray mass spectra of homogeneous preparations formed from three different phospholipid molecules demonstrate that under certain conditions interactions between 90 and 100 lipid molecules can be preserve...

journal_title：Biophysical journal

authors： Hanson CL,Ilag LL,Malo J,Hatters DM,Howlett GJ,Robinson CV

更新日期：2003-12-01 00:00:00

abstract：:Small, icosahedral double-stranded DNA bacteriophage pack their genomes tightly into preformed protein capsids using an ATP-driven motor. Coarse-grain molecular-mechanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformation depends on capsid size and shape, and the presence o...

journal_title：Biophysical journal

authors： Petrov AS,Harvey SC

更新日期：2008-07-01 00:00:00

abstract：:Biofilm infections can consist of bacterial aggregates that are an order of magnitude larger than neutrophils, phagocytic immune cells that densely surround aggregates but do not enter them. Because a neutrophil is too small to engulf the entire aggregate, it must be able to detach and engulf a few bacteria at a time ...

journal_title：Biophysical journal

authors： Davis-Fields M,Bakhtiari LA,Lan Z,Kovach KN,Wang L,Cosgriff-Hernandez EM,Gordon VD

更新日期：2019-10-15 00:00:00

abstract：:A systematic new approach to derive multiscale coarse-grained (MS-CG) models has been recently developed. The approach employs information from atomistically detailed simulations to derive CG forces and associated effective potentials. In this work, the MS-CG methodology is extended to study two peptides representing ...

journal_title：Biophysical journal

authors： Zhou J,Thorpe IF,Izvekov S,Voth GA

更新日期：2007-06-15 00:00:00

abstract：:Tropomyosin (Tm) paracrystal formation induced by Mg2+ was studied by monitoring increases in light scattering. Paracrystals formed above a critical Tm concentration with lag phases in the time courses at pH 7.5 and 6.0, indicating that condensation polymerization processes are involved. The kinetic data at pH 7.5 rea...

journal_title：Biophysical journal

authors： Ishii Y,Lehrer SS

更新日期：1989-07-01 00:00:00

abstract：:Homooligomerization of proline utilization A (PutA) bifunctional flavoenzymes is intimately tied to catalytic function and substrate channeling. PutA from Bradyrhizobium japonicum (BjPutA) is unique among PutAs in that it forms a tetramer in solution. Curiously, a dimeric BjPutA hot spot mutant was previously shown to...

journal_title：Biophysical journal

authors： Korasick DA,Campbell AC,Christgen SL,Chakravarthy S,White TA,Becker DF,Tanner JJ

更新日期：2018-06-19 00:00:00

abstract：:We have developed a saturation transfer EPR (ST-EPR) method to measure selectively the rotational dynamics of those lipids that are motionally restricted by integral membrane proteins and have applied this methodology to measure lipid-protein interactions in native sarcoplasmic reticulum (SR) membranes. This analysis ...

journal_title：Biophysical journal

authors： Squier TC,Thomas DD

更新日期：1989-10-01 00:00:00

abstract：:Photoinduced electron transfer in photosystem I (PS I) proceeds from the excited primary electron donor P700 (a chlorophyll a dimer) via the primary acceptor A0 (chlorophyll a) and the secondary acceptor A1 (phylloquinone) to three [4Fe-4S] clusters, Fx, FA, and FB. Prereduction of the iron-sulfur clusters blocks elec...

journal_title：Biophysical journal

authors： Polm M,Brettel K

更新日期：1998-06-01 00:00:00