Abstract:
:Cyclic-nucleotide-activated, nonselective cation channels have a central role in sensory transduction. They are most likely tetramers, composed of two subunits (alpha and beta or 1 and 2), with the former, but not the latter, being able to form homomeric cyclic-nucleotide-activated channels. Identified members of this channel family now include, in vertebrates, the rod and cone channels mediating visual transduction and the channel mediating olfactory transduction, each apparently with distinct alpha- and beta-subunits. Homologous channels have also been identified in Drosophila melanogaster and Caenorhabditis elegans. By co-expressing any combination of two alpha-subunits, or alpha- and beta-subunits, of this channel family in HEK 293 cells, we have found that they can all co-assemble functionally with each other, including those from fly and nematode. This finding suggests that the subunit members so far identified form a remarkably homogeneous and conserved group, functionally and evolutionarily, with no subfamilies yet identified. The ability to cross-assemble allows these subunits to potentially generate a diversity of heteromeric channels, each with properties specifically suited to a particular cellular function.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Finn JT,Krautwurst D,Schroeder JE,Chen TY,Reed RR,Yau KWdoi
10.1016/S0006-3495(98)77846-4subject
Has Abstractpub_date
1998-03-01 00:00:00pages
1333-45issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(98)77846-4journal_volume
74pub_type
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