Differential effects of amino-terminal distal and proximal domains in the regulation of human erg K(+) channel gating.

abstract：:We have used confocal microscopy to examine the [Ca2+]i increase in the albino eggs of the frog Xenopus laevis after fertilization. Eggs were placed in agar wells with their animal poles downward so that fertilization occurred preferentially in the equatorial plane, and confocal microscopy was used to provide a two-di...

journal_title：Biophysical journal

authors： Fontanilla RA,Nuccitelli R

更新日期：1998-10-01 00:00:00

abstract：:Giant vesicles formed of 1,2-dipalmitoylphosphatidylcholine (DPPC) and sterols (cholesterol or ergosterol) in water and water/ethanol solutions have been used to examine the effect of sterol composition and ethanol concentration on the area compressibility modulus (K(a)), overall mechanical behavior, vesicle morpholog...

journal_title：Biophysical journal

authors： Tierney KJ,Block DE,Longo ML

更新日期：2005-10-01 00:00:00

abstract：:Crossover motifs are integral components for designing DNA-based nanostructures and nanomechanical devices due to their enhanced rigidity compared to the normal B-DNA. Although the structural rigidity of the double helix B-DNA has been investigated extensively using both experimental and theoretical tools, to date the...

journal_title：Biophysical journal

authors： Santosh M,Maiti PK

更新日期：2011-09-21 00:00:00

abstract：:Differentiation from a multipotent stem or progenitor state to a mature cell is an essentially irreversible process. The associated changes in gene expression patterns exhibit time-directionality. This "arrow of time" in the collective change of gene expression across multiple stable gene expression patterns (attracto...

journal_title：Biophysical journal

authors： Wang J,Xu L,Wang E,Huang S

更新日期：2010-07-07 00:00:00

abstract：:A Brownian dynamics (BD) model described in the accompanying paper (Klenin, K., H. Merlitz, and J. Langowski. 1998. A Brownian dynamics program for the simulation of linear and circular DNA, and other wormlike chain polyelectrolytes. Biophys. J. 74:000-000) has been used for computing the end-to-end distance distribut...

journal_title：Biophysical journal

authors： Merlitz H,Rippe K,Klenin KV,Langowski J

更新日期：1998-02-01 00:00:00

abstract：:The cell membrane of Valonia utricularis contains an electrogenic carrier system for chloride (Wang et al., Biophys J. 59:235-248 (1991)). The electrical impedance of V. utricularis was measured in the frequency range between 1 Hz and 50 kHz. The analysis of the impedance spectra from V. utricularis and its comparison...

journal_title：Biophysical journal

authors： Wang J,Zimmermann U,Benz R

更新日期：1994-10-01 00:00:00

abstract：:Nanopatterned surfaces are believed to kill bacteria through physical deformation, a mechanism that has immense potential against biochemical resistance. Because of its elusive nature, this mechanism is mostly understood through biophysical modeling. Problematically, accurate descriptions of the contact mechanics and ...

journal_title：Biophysical journal

authors： Velic A,Hasan J,Li Z,Yarlagadda PKDV

更新日期：2021-01-19 00:00:00

abstract：:The effect of weak, nonspecific interaction between molecules confined within restricted elements of volume ("pores") and the boundary surfaces of the pore, upon the reactivity and physical state of the confined molecules, is explored by means of simple models. A confined molecule is represented by a rectangular paral...

journal_title：Biophysical journal

authors： Minton AP

更新日期：1995-04-01 00:00:00

abstract：:The chemical unfolding transition of a protein was simulated, including the presence of an intermediate (I) in equilibrium with the native (N) and unfolded (U) states. The calculations included free energies of unfolding, DeltaGuw, in the range of 1.4 kcal/mol to 10 kcal/mol and three different global m-values. The si...

journal_title：Biophysical journal

authors： Soulages JL

更新日期：1998-07-01 00:00:00

abstract：:Computational methods that predict three-dimensional structures from amino acid sequences have become increasingly accurate and have provided insights into structure-function relationships for proteins in the absence of structural data. However, the accuracy of computational structural models requires experimental app...

journal_title：Biophysical journal

authors： Tateyama M,Liu H,Yang AS,Cormier JW,Kass RS

更新日期：2004-03-01 00:00:00

abstract：:Adaptation of the attractant response in Escherichia coli is attributable to the methylation of its transmembrane chemotactic receptors by the methyltransferase CheR. This protein contains two binding domains, one for the sites of methylation themselves and the other for a flexible tether at the C terminus of the rece...

journal_title：Biophysical journal

authors： Levin MD,Shimizu TS,Bray D

更新日期：2002-04-01 00:00:00

abstract：:Molecular dynamics simulations of protein tyrosine phosphatase 1B (PTP1B) complexed with the phosphorylated peptide substrate DADEpYL and the free substrate have been conducted to investigate 1) the physical forces involved in substrate-protein interactions, 2) the importance of enzyme and substrate flexibility for bi...

journal_title：Biophysical journal

authors： Peters GH,Frimurer TM,Andersen JN,Olsen OH

更新日期：2000-05-01 00:00:00

abstract：:This work presents a methodology to measure and quantitatively interpret force curves on supported lipid bilayers in water. We then use this method to correlate topographic imaging contrast in atomic force microscopy (AFM) images of phase-separated Langmuir-Blodgett bilayers with imaging load. Force curves collected o...

journal_title：Biophysical journal

authors： Schneider J,Dufrêne YF,Barger WR Jr,Lee GU

更新日期：2000-08-01 00:00:00

abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

journal_title：Biophysical journal

authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

更新日期：2010-10-20 00:00:00

abstract：:To understand better the effect of electrostatics on the rigidity of the DNA double helix, we define DNA*, the null isomer of DNA, as the hypothetical structure that would result from DNA if its phosphate groups were not ionized. For the purposes of theoretical analysis, we model DNA* as identical to ordinary DNA but ...

journal_title：Biophysical journal

authors： Manning GS

更新日期：2006-11-15 00:00:00

abstract：:The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidopho...

journal_title：Biophysical journal

authors： Malewicz B,Valiyaveettil JT,Jacob K,Byun HS,Mattjus P,Baumann WJ,Bittman R,Brown RE

更新日期：2005-04-01 00:00:00

abstract：:Protein-mediated membrane remodeling is a ubiquitous and critical process for proper cellular function. Inverse Bin/Amphiphysin/Rvs (I-BAR) domains drive local membrane deformation as a precursor to large-scale membrane remodeling. We employ a multiscale approach to provide the molecular mechanism of unusual I-BAR dom...

journal_title：Biophysical journal

authors： Jarin Z,Tsai FC,Davtyan A,Pak AJ,Bassereau P,Voth GA

更新日期：2019-08-06 00:00:00

abstract：:It has been a long belief that release of substances from the cell to the extracellular milieu by exocytosis is completed by diffusion of the substances from secretory vesicles through the fusion pore. Involvement of any mechanical force that may be superposed on the diffusion to enhance the releasing process has not ...

journal_title：Biophysical journal

authors： Tsuboi T,Kikuta T,Sakurai T,Terakawa S

更新日期：2002-07-01 00:00:00

abstract：:The presence of tryptophan residues as intrinsic fluorophores in most proteins makes them an obvious choice for fluorescence spectroscopic analyses of such proteins. Membrane proteins have been reported to have a significantly higher tryptophan content than soluble proteins. The role of tryptophan residues in the stru...

journal_title：Biophysical journal

authors： Chattopadhyay A,Mukherjee S,Rukmini R,Rawat SS,Sudha S

更新日期：1997-08-01 00:00:00

abstract：:Single actin filaments were analyzed in solutions ranging from dilute (0.2 microgram/ml), where filaments interact only with solvent, to concentrations (4.0 mg/ml) at which F-actin forms a nematic phase. A persistence length of approximately 1.8 microns and an average length of approximately 22 microns (Kaufmann et al...

journal_title：Biophysical journal

authors： Käs J,Strey H,Tang JX,Finger D,Ezzell R,Sackmann E,Janmey PA

更新日期：1996-02-01 00:00:00

abstract：:Isothermal (27 degrees C) phase behavior of dimyristoyl phosphatidyl choline-cholesterol mixtures at various osmotic pressures and cholesterol contents was investigated by means of isothermal sorption microcalorimetry and (2)H-nuclear magnetic resonance. The calorimetric method allows for simultaneous measurement of t...

journal_title：Biophysical journal

authors： Sparr E,Hallin L,Markova N,Wennerström H

更新日期：2002-10-01 00:00:00

abstract：:Knowledge about the influenza fusion peptide (FP) membrane insertion mode is crucial for understanding its fusogenic mechanism. NMR and electron paramagnetic resonance experiments showed that in micelles, the FP inserted as a fixed-angle inverted V. In membranes, however, it was shown to insert as a straight α-helix (...

journal_title：Biophysical journal

authors： Légaré S,Lagüe P

更新日期：2012-05-16 00:00:00

abstract：:Although ubiquitous, the processes by which bacteria colonize surfaces remain poorly understood. Here we report results for the influence of the wall shear stress on the early-stage adhesion of Pseudomonas aeruginosa PA14 on glass and polydimethylsiloxane surfaces. We use image analysis to measure the residence time o...

journal_title：Biophysical journal

authors： Lecuyer S,Rusconi R,Shen Y,Forsyth A,Vlamakis H,Kolter R,Stone HA

更新日期：2011-01-19 00:00:00

abstract：:The effects of rigor and cycling cross-bridges on distributions of calcium (Ca) bound within sarcomeres of rabbit psoas muscle fibers were compared using electron probe x-ray microanalysis. Calcium in the overlap region of rigor fibers, after correction for that bound to thick filaments, was significantly higher than ...

journal_title：Biophysical journal

authors： Cantino ME,Quintanilla A

更新日期：2007-01-15 00:00:00

abstract：:We argue that membrane viscosity, η(m), plays a prominent role in the thermal fluctuation dynamics of micron-scale lipid domains. A theoretical expression is presented for the timescales of domain shape relaxation, which reduces to the well-known η(m) = 0 result of Stone and McConnell in the limit of large domain size...

journal_title：Biophysical journal

authors： Camley BA,Esposito C,Baumgart T,Brown FL

更新日期：2010-09-22 00:00:00

abstract：:Cryoelectron microscopy has been used to study the reorganization of unilamellar cationic lipid vesicles upon the addition of DNA. Unilamellar DNA-coated vesicles, as well as multilamellar DNA lipid complexes, could be observed. Also, DNA induced fusion of unilamellar vesicles was found. DNA appears to adsorb to the o...

journal_title：Biophysical journal

authors： Huebner S,Battersby BJ,Grimm R,Cevc G

更新日期：1999-06-01 00:00:00

abstract：:Histograms of single-molecule Förster resonance energy transfer (FRET) efficiency are often used to study the structures of biomolecules and relate these structures to function. Methods like probability distribution analysis analyze FRET histograms to detect heterogeneities in molecular structure, but they cannot dete...

journal_title：Biophysical journal

authors： Torella JP,Holden SJ,Santoso Y,Hohlbein J,Kapanidis AN

更新日期：2011-03-16 00:00:00

abstract：:Deuterium NMR spectroscopy was used to study how the positioning of a dimerization motif within a transbilayer polypeptide influences its orientation and dynamics in bilayers. Three polypeptide variants comprising glycophorin A transmembrane (GpATM) dimerization motifs incorporated into lysine-terminated poly-leucine-...

journal_title：Biophysical journal

authors： McDonald MC,Booth V,Morrow MR

更新日期：2011-02-02 00:00:00

abstract：:The cytoplasm of a living cell is a dynamic environment through which intracellular components must move and mix. In motile, rapidly deforming cells such as human neutrophils, bulk cytoplasmic flow couples cell deformation to the transport and dispersion of cytoplasmic particles. Using particle-tracking measurements i...

journal_title：Biophysical journal

authors： Koslover EF,Chan CK,Theriot JA

更新日期：2017-11-07 00:00:00

abstract：:ATP-sensitive K(+)-channels link metabolism and excitability in neurons, myocytes, and pancreatic islets. Mutations in the pore-forming subunit (Kir6.2; KCNJ11) cause neonatal diabetes, developmental delay, and epilepsy by decreasing sensitivity to ATP inhibition and suppressing electrical activity. Mutations of resid...

journal_title：Biophysical journal

authors： Koster JC,Kurata HT,Enkvetchakul D,Nichols CG

更新日期：2008-11-15 00:00:00