The influenza fusion peptide adopts a flexible flat V conformation in membranes.

abstract：:The pKa values of ionizable groups that lie between the active site region of bacteriorhodopsin (bR) and the extracellular surface of the protein are reported. Glu-204 is found to have an elevated pKa in the resting state of bR, suggesting that it corresponds to the proton-releasing group in bR. Its elevated pKa is pr...

journal_title：Biophysical journal

authors： Sampogna RV,Honig B

更新日期：1996-09-01 00:00:00

abstract：:Total internal reflection excitation used in combination with fluorescence correlation spectroscopy (TIR-FCS) is a method for characterizing the dynamic behavior and absolute concentrations of fluorescent molecules near or at the interface of a planar substrate and a solution. In this work, we demonstrate for the firs...

journal_title：Biophysical journal

authors： Lieto AM,Cush RC,Thompson NL

更新日期：2003-11-01 00:00:00

abstract：:In previous in vivo studies, amyloid fibers formed from a peptide ubiquitous in human seminal fluid (semen-derived enhancer of viral infection (SEVI)) were found to dramatically enhance the infectivity of the HIV virus (3-5 orders of magnitude by some measures). To complement those studies, we performed in vitro assay...

journal_title：Biophysical journal

authors： Brender JR,Hartman K,Gottler LM,Cavitt ME,Youngstrom DW,Ramamoorthy A

更新日期：2009-11-04 00:00:00

abstract：:The absence of comprehensive measured kinetic values and the observed inconsistency in the available in vitro kinetic data has hindered the formulation of network-scale kinetic models of biochemical reaction networks. To meet this challenge we present an approach to construct a convex space, termed the k-cone, which c...

journal_title：Biophysical journal

authors： Famili I,Mahadevan R,Palsson BO

更新日期：2005-03-01 00:00:00

abstract：:The elastic rigidity (stiffness) of impaled motionless bull sperm flagella has been determined by a manipulatory technique which permitted direct analytical treatment of the experimental system. The effects of external ATP and ADP were measured. It was found that ATP acts as a plasticizing agent, while ADP does not. T...

journal_title：Biophysical journal

authors： Lindemann CB,Rudd WG,Rikmenspoel R

更新日期：1973-05-01 00:00:00

abstract：:The morphological features of α-synuclein (AS) amyloid aggregation in vitro and in cells were elucidated at the nanoscale by far-field subdiffraction fluorescence localization microscopy. Labeling AS with rhodamine spiroamide probes allowed us to image AS fibrillar structures by fluorescence stochastic nanoscopy with ...

journal_title：Biophysical journal

authors： Roberti MJ,Fölling J,Celej MS,Bossi M,Jovin TM,Jares-Erijman EA

更新日期：2012-04-04 00:00:00

abstract：:This paper examines "realistic" pores, i.e., ones that are neither symmetric nor of uniform diameter. Methods are described that permit estimation of the image potential for an ion in an aqueous pore spanning a lipid membrane and for the electric field produced in such a pore when a transmembrane potential is applied....

journal_title：Biophysical journal

authors： Jordan PC

更新日期：1984-06-01 00:00:00

abstract：:An understanding of the structure-dynamics relationship is essential for understanding how a protein works. Prior research has shown that the activity of a protein correlates with intradomain dynamics occurring at picosecond to millisecond timescales. However, the correlation between interdomain dynamics and the funct...

journal_title：Biophysical journal

authors： Kumar L,Nash A,Harms C,Planas-Iglesias J,Wright D,Klein-Seetharaman J,Sarkar SK

更新日期：2020-07-21 00:00:00

abstract：:The present article considers the influence of heterogeneity in a mobile analyte or in an immobilized ligand population on the surface binding kinetics and equilibrium isotherms. We describe strategies for solving the inverse problem of calculating two-dimensional distributions of rate and affinity constants from expe...

journal_title：Biophysical journal

authors： Svitel J,Balbo A,Mariuzza RA,Gonzales NR,Schuck P

更新日期：2003-06-01 00:00:00

abstract：:The reaction of c-cytochromes with iron hexacyanides is similar in mechanism to the interaction of cytochromes with their physiological oxidants and reductants in that the formation of complexes precedes electron transfer. Analysis of the kinetics of oxidation and reduction of a number of c-cytochromes by solving the ...

journal_title：Biophysical journal

authors： Ohno N,Cusanovich MA

更新日期：1981-12-01 00:00:00

abstract：:Force spectroscopy is commonly used to measure the kinetics of processes occurring in single biological molecules. These measurements involve attaching the molecule of interest to micron-sized or larger force probes via compliant linkers. Recent theoretical work has described how the properties of the probes and linke...

journal_title：Biophysical journal

authors： Neupane K,Woodside MT

更新日期：2016-07-26 00:00:00

abstract：:We present a molecular system where polymerization is controlled externally by tuning the elastic energy of the monomers. The elastic energy, provided by a DNA molecular spring, destabilizes the monomer state through a process analogous to domain swapping. This energy can be large (of approximately 10 kT) and thus dri...

journal_title：Biophysical journal

authors： Wang A,Zocchi G

更新日期：2009-03-18 00:00:00

abstract：:We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing ...

journal_title：Biophysical journal

authors： Chiantia S,Schwille P,Klymchenko AS,London E

更新日期：2011-01-05 00:00:00

abstract：:We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force...

journal_title：Biophysical journal

authors： Ounkomol C,Yamada S,Heinrich V

更新日期：2010-12-15 00:00:00

abstract：:Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here, we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay with multicolor single-molecule fluorescence. We demons...

journal_title：Biophysical journal

authors： Severins I,Szczepaniak M,Joo C

更新日期：2018-09-18 00:00:00

abstract：:In this paper, we report on the presence of cation binding areas on bovine serum amine oxidase, where metal ions of the groups IA and IIA, such as Na(+), K(+), Cs(+), Mg(2+), and Ca(2+), bind with various affinities. We found a cation-binding area that influences the enzyme activity if occupied, so that the catalytic ...

journal_title：Biophysical journal

authors： Di Paolo ML,Scarpa M,Corazza A,Stevanato R,Rigo A

更新日期：2002-10-01 00:00:00

abstract：:Quantum chemical computations using the ab initio molecular orbital (MO) method have been performed to investigate the molecular switching mechanism of Ras protein p21, which has an important role in intracellular signal cascades. Lys(16) was demonstrated to be crucial to the function of Ras p21, and the hydrolysis of...

journal_title：Biophysical journal

authors： Futatsugi N,Hata M,Hoshino T,Tsuda M

更新日期：1999-12-01 00:00:00

abstract：:Secondary structure formation of nucleic acids strongly depends on salt concentration and temperature. We develop a theory for RNA folding that correctly accounts for sequence effects, the entropic contributions associated with loop formation, and salt effects. Using an iterative expression for the partition function ...

journal_title：Biophysical journal

authors： Einert TR,Netz RR

更新日期：2011-06-08 00:00:00

abstract：:Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligo...

journal_title：Biophysical journal

authors： Pannwitt S,Stangl M,Schneider D

更新日期：2019-11-05 00:00:00

abstract：:We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments sh...

journal_title：Biophysical journal

authors： Hsueh YW,Weng CJ,Chen MT,Thewalt J,Zuckermann M

更新日期：2010-04-07 00:00:00

abstract：:The thylakoid membrane has a unique lipid composition, consisting mostly of galactolipids. These thylakoid lipids have important roles in photosynthesis. Here, we investigate to what extent these lipids bind specifically to the Photosystem II complex. To this end, we performed coarse-grain MD simulations of the Photos...

journal_title：Biophysical journal

authors： Van Eerden FJ,Melo MN,Frederix PWJM,Marrink SJ

更新日期：2017-12-19 00:00:00

abstract：:K(ATP) channels are comprised of a pore-forming protein, Kir6.x, and the sulfonylurea receptor, SURx. Interaction of adenine nucleotides with Kir6.2 positively charged amino acids such as K185 and R201 on the C-terminus causes channel closure. Substitution of these amino acids with other positively charged residues ha...

journal_title：Biophysical journal

authors： Ribalet B,John SA,Weiss JN

更新日期：2003-01-01 00:00:00

abstract：:We used time-resolved Förster resonance energy transfer, circular dichroism, and molecular dynamics simulation to investigate the structural dependence of synaptotagmin 1's intrinsically disordered region (IDR) on phosphorylation and dielectric constant. We found that a peptide corresponding to the full-length IDR seq...

journal_title：Biophysical journal

authors： Fealey ME,Binder BP,Uversky VN,Hinderliter A,Thomas DD

更新日期：2018-02-06 00:00:00

abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

journal_title：Biophysical journal

authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

更新日期：1979-02-01 00:00:00

abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

journal_title：Biophysical journal

authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

更新日期：2010-07-21 00:00:00

abstract：:Ceramides have been implicated in the initiation of apoptosis by permeabilizing the mitochondrial outer membrane to small proteins, including cytochrome c. In addition, ceramides were shown to form large metastable channels in planar membranes and liposomes, indicating that these lipids permeabilize membranes directly...

journal_title：Biophysical journal

authors： Anishkin A,Sukharev S,Colombini M

更新日期：2006-04-01 00:00:00

abstract：:Understanding cellular membrane processes is critical for the study of events such as viral entry, neurotransmitter exocytosis, and immune activation. Supported lipid bilayers are commonly used to model these membrane processes experimentally. Despite the relative simplicity of such a system, many important structural...

journal_title：Biophysical journal

authors： Kasson PM,Pande VS

更新日期：2004-06-01 00:00:00

abstract：:Many multicellular communities propagate signals in a directed manner via excitable waves. Cell-to-cell heterogeneity is a ubiquitous feature of multicellular communities, but the effects of heterogeneity on wave propagation are still unclear. Here, we use a minimal FitzHugh-Nagumo-type model to investigate excitable ...

journal_title：Biophysical journal

authors： Zhai X,Larkin JW,Süel GM,Mugler A

更新日期：2020-04-07 00:00:00

abstract：:Pore formation by membrane-active peptides, naturally encountered in innate immunity and infection, could have important medical and technological applications. Recently, the well-studied lytic peptide melittin has formed the basis for the development of combinatorial libraries from which potent pore-forming peptides ...

journal_title：Biophysical journal

authors： Sepehri A,PeBenito L,Pino-Angeles A,Lazaridis T

更新日期：2020-04-21 00:00:00

abstract：:It is shown that alpha-helical content of eleven proteins is well correlated with alanine plus leucine content. These residues, taken singly or together, are to a first approximation randomly distributed in the four proteins whose tertiary structures have been determined (i.e., myoglobin, lysozyme, ribonuclease, alpha...

journal_title：Biophysical journal

authors： Prothero JW

更新日期：1968-11-01 00:00:00