Abstract:
:We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force microscope with micropipette manipulation. It enables us to test multiple biomolecular interactions of the same cell in a swift sequential or cyclic manner and thus to resolve subtle differences between individual interactions that otherwise would be obscured by cell-cell baseline variability. For each cell, we contrast heterophilic E:N-cadherin binding with the respective homophilic bonds and with a suitable control. Clarifying previous literature reports, we establish that specific bonds between E- and N-cadherins form readily, albeit less frequently than homophilic bonds of either cadherin. We support this assessment with a rough estimate of the ratio of on-rate constants of E/N-cadherin binding.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Ounkomol C,Yamada S,Heinrich Vdoi
10.1016/j.bpj.2010.11.013subject
Has Abstractpub_date
2010-12-15 00:00:00pages
L100-2issue
12eissn
0006-3495issn
1542-0086pii
S0006-3495(10)01382-2journal_volume
99pub_type
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