Self-association of models of transmembrane domains of ErbB receptors in a lipid bilayer.

abstract：:A single isolated sinoatrial pacemaker cell presents intrinsic interbeat interval (IBI) variability that is believed to result from the stochastic characteristics of the opening and closing processes of membrane ion channels. To our knowledge, a novel mathematical framework was developed in this work to address the ef...

journal_title：Biophysical journal

authors： Dvir H,Zlochiver S

更新日期：2015-03-10 00:00:00

abstract：:Spin inversion transfer (SIT) NMR experiments are reported probing the thermodynamics and kinetics of interconversion of two folded forms of a GCN4-like leucine zipper near room temperature. The peptide is 13Calpha-labeled at position V9(a) and results are compared with prior findings for position L13(e). The SIT data...

journal_title：Biophysical journal

authors： d'Avignon DA,Bretthorst GL,Holtzer ME,Holtzer A

更新日期：1999-05-01 00:00:00

abstract：:A number of models proposed to account for the sodium conductance changes are shown to fall into two classes. The Hodgkin-Huxley (HH) model falls into a class (I) in which the conductance depends on two or more independent variables controlled by independent processes. The Mullins, Hoyt, and Goldman models fall into c...

journal_title：Biophysical journal

authors： Hoyt RC

更新日期：1968-10-01 00:00:00

abstract：:The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...

journal_title：Biophysical journal

authors： Lacroix JJ,Bezanilla F

更新日期：2012-08-08 00:00:00

abstract：:Cell-reorganized fibronectin layers on polymer films providing a gradation of the binding strength between protein and substrate were analyzed by combined fluorescence and scanning force microscopy. The nanoscale fibronectin patterns exhibited paired parallel fibrils with characteristic spacings of 156, 233, 304, and ...

journal_title：Biophysical journal

authors： Pompe T,Renner L,Werner C

更新日期：2005-01-01 00:00:00

abstract：:Equations are derived describing the dispersion of a permeable solute during Poiseuille flow in a capillary model. It is shown that for the normal range of physiological parameters such as capillary radius, capillary length, blood flow, permeability coefficients, and diffusion constants, the center of mass of a bolus ...

journal_title：Biophysical journal

authors： Pollock F,Blum JJ

更新日期：1966-01-01 00:00:00

abstract：:P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of intracellular materials from the damaged cells. To explore the nature of the interaction between P188 and cell membranes, we have constructed a model...

journal_title：Biophysical journal

authors： Maskarinec SA,Hannig J,Lee RC,Lee KY

更新日期：2002-03-01 00:00:00

abstract：:Xenopus oocytes are widely employed for heterologous expression of cloned proteins, particularly electrogenic molecules such as ion channels and transporters. The high levels of expression readily obtained permit detailed investigations without interference from endogenous conductances. Injection of min K mRNA into Xe...

journal_title：Biophysical journal

authors： Tzounopoulos T,Maylie J,Adelman JP

更新日期：1995-09-01 00:00:00

abstract：:We have used fluorescence-quenching measurements to characterize the partitioning of a variety of indolyl-labeled phospho- and sphingolipids between gel or liquid-ordered and liquid-disordered lipid domains in several types of lipid bilayers where such domains coexist. In both cholesterol-free and cholesterol-containi...

journal_title：Biophysical journal

authors： Wang TY,Silvius JR

更新日期：2003-01-01 00:00:00

abstract：:The one-dimensional information of genomic DNA is hierarchically packed inside the eukaryotic cell nucleus and organized in a three-dimensional (3D) space. Genome-wide chromosome conformation capture (Hi-C) methods have uncovered the 3D genome organization and revealed multiscale chromatin domains of compartments and ...

journal_title：Biophysical journal

authors： Shinkai S,Sugawara T,Miura H,Hiratani I,Onami S

更新日期：2020-05-05 00:00:00

abstract：:A new Fourier series representation of supercoiled DNA is employed in Langevin dynamics simulations to study large-scale configurational motions of intermediate-length chains. The polymer is modeled as an ideal elastic rod subject to long-range van der Waals' interactions. The van der Waals' term prevents the self-con...

journal_title：Biophysical journal

authors： Liu G,Schlick T,Olson AJ,Olson WK

更新日期：1997-10-01 00:00:00

abstract：:Neuronal synapses are highly specialized structures for communication between nerve cells. Knowledge about their molecular organization and dynamics is still incomplete. The large multidomain protein Bassoon plays a major role in scaffolding and organizing the cytomatrix at the active zone of neurotransmitter release ...

journal_title：Biophysical journal

authors： Jose M,Nair DK,Altrock WD,Dresbach T,Gundelfinger ED,Zuschratter W

更新日期：2008-02-15 00:00:00

abstract：:The potassium selective, inward rectifier current (IK1) is known to be responsible for maintaining the resting membrane potential of quiescent ventricular myocytes. However, the contribution of this current to the different phases of the cardiac action potential has not been adequately established. In the present stud...

journal_title：Biophysical journal

authors： Ibarra J,Morley GE,Delmar M

更新日期：1991-12-01 00:00:00

abstract：:We propose mechanisms by which the transmembrane domain of vesicular stomatitis virus (VSV-TMD) promotes both initiation of fusion and formation of a fusion pore. Time courses of polyethyleneglycol (PEG)-mediated fusion of 25 nm small unilamellar vesicles composed of dioleoylphosphatidylcholine, dioleoylphosphatidylet...

journal_title：Biophysical journal

authors： Sengupta T,Chakraborty H,Lentz BR

更新日期：2014-09-16 00:00:00

abstract：:Na(+) conductance through cloned K(+) channels has previously allowed characterization of inactivation and K(+) binding within the pore, and here we have used Na(+) permeation to study recovery from C-type inactivation in human Kv1.5 channels. Replacing K(+) in the solutions with Na(+) allows complete Kv1.5 inactivati...

journal_title：Biophysical journal

authors： Wang Z,Hesketh JC,Fedida D

更新日期：2000-11-01 00:00:00

abstract：:A new method, weighted-ensemble Brownian dynamics, is proposed for the simulation of protein-association reactions and other events whose frequencies of outcomes are constricted by free energy barriers. The method features a weighted ensemble of trajectories in configuration space with energy levels dictating the prop...

journal_title：Biophysical journal

authors： Huber GA,Kim S

更新日期：1996-01-01 00:00:00

abstract：:Sinusoidal oscillatory flow of blood and of aqueous glycerol solutions was produced in rigid cylindrical tubes. For aqueous glycerol, the amplitude of the measured pressure gradient wave form conformed closely to that predicted by Womersley's theory of oscillatory flow, up to Reynolds numbers approaching 2000. Blood d...

journal_title：Biophysical journal

authors： Kunz AL,Coulter NA Jr

更新日期：1967-01-01 00:00:00

abstract：:The oligomerization of the plasma membrane calcium pump (PMCA) in phospholipid/detergent micelles was evaluated using a combined spectroscopic and kinetic approach and related to the enzyme stability. Energy transfer between fluorescein-5'-isothiocyanate and eosin-5'-isothiocyanate attached to different PMCA molecules...

journal_title：Biophysical journal

authors： Levi V,Rossi JP,Castello PR,González Flecha FL

更新日期：2002-01-01 00:00:00

abstract：:The oligomeric state and function of band 3, purified by sulfhydryl affinity chromatography in reduced Triton X-100, was investigated. Size exclusion high-performance liquid chromatography showed that a homogeneous population of band 3 dimers could be purified from whole erythrocyte membranes. The elution profile of b...

journal_title：Biophysical journal

authors： Taylor AM,Boulter J,Harding SE,Cölfen H,Watts A

更新日期：1999-04-01 00:00:00

abstract：:The energy transfer processes between carotenoids and Chls have been studied by femtosecond transient absorption in the CP29-WT complex, which contains only two carotenoids per polypeptide located in the L1 and L2 sites, and in the CP29-E166V mutant in which only the L1 site is occupied. The comparison of these two sa...

journal_title：Biophysical journal

authors： Croce R,Müller MG,Caffarri S,Bassi R,Holzwarth AR

更新日期：2003-04-01 00:00:00

abstract：:Cholesterol is a key component of eukaryotic membranes, but its role in cellular biology in general and in lipid rafts in particular remains controversial. Model membranes are used extensively to determine the phase behavior of ternary mixtures of cholesterol, a saturated lipid, and an unsaturated lipid with liquid-or...

journal_title：Biophysical journal

authors： Bennett WFD,Shea JE,Tieleman DP

更新日期：2018-06-05 00:00:00

abstract：:The general theory (Levitt, D. G. 1990. Biophys. J. 59:271-277) is applied to a model channel that resembles the acetylcholine receptor channel (ACH). The model incorporates the known features of the ACH geometry and fixed charge locations. The channel has a wide mouth facing the outer solution, tapering to a narrow r...

journal_title：Biophysical journal

authors： Levitt DG

更新日期：1991-02-01 00:00:00

abstract：:Exposure of aqueous suspensions of fragments of the purple membrane of Halobacterium halobium to electric field pulses leads to transient linear dichroism phenomena. The effects are interpreted in terms of field-induced alignments of the bacteriorhodopsin chromophore. Two observed relaxation times (tau) are attributed...

journal_title：Biophysical journal

authors： Shinar R,Druckmann S,Ottolenghi M,Korenstein R

更新日期：1977-07-01 00:00:00

abstract：:Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates ...

journal_title：Biophysical journal

authors： Hu J,Asbury T,Achuthan S,Li C,Bertram R,Quine JR,Fu R,Cross TA

更新日期：2007-06-15 00:00:00

abstract：:A complete mechanistic understanding of membrane-localized processes in vesicular transport, such as membrane budding and fission, requires their reconstitution with biochemically-defined components from a biochemically-defined substrate. Supported bilayers formed by vesicle fusion represent an attractive substrate fo...

journal_title：Biophysical journal

authors： Pucadyil TJ,Schmid SL

更新日期：2010-07-21 00:00:00

abstract：:Steady-state and time-resolved fluorescence spectroscopy was used to follow the local and global changes in structure and dynamics during chemical and thermal denaturation of unlabeled human serum albumin (HSA) and HSA with an acrylodan moiety bound to Cys34. Acrylodan fluorescence was monitored to obtain information ...

journal_title：Biophysical journal

authors： Flora K,Brennan JD,Baker GA,Doody MA,Bright FV

更新日期：1998-08-01 00:00:00

abstract：:We present an analysis of physical chemical constraints on the accuracy of DNA micro-arrays under equilibrium and nonequilibrium conditions. At the beginning of the article we describe an algorithm for choosing a probe set with high specificity for targeted genes under equilibrium conditions. The algorithm as well as ...

journal_title：Biophysical journal

authors： Bhanot G,Louzoun Y,Zhu J,DeLisi C

更新日期：2003-01-01 00:00:00

abstract：:The three-dimensional structure of chromatin affects DNA accessibility and is therefore a key regulator of gene expression. However, the path of the DNA between consecutive nucleosomes, and the resulting chromatin fiber organization remain controversial. The conformational space available for the folding of the nucleo...

journal_title：Biophysical journal

authors： Stehr R,Schöpflin R,Ettig R,Kepper N,Rippe K,Wedemann G

更新日期：2010-03-17 00:00:00

abstract：:Xenopus melanophores have pigment organelles or melanosomes which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. Melanosomes are transported by microtubule motors, kinesin-2 and cytoplasmic dynein, and an actin motor, myosin-V. We explored the regulation of melanosome trans...

journal_title：Biophysical journal

authors： Levi V,Serpinskaya AS,Gratton E,Gelfand V

更新日期：2006-01-01 00:00:00

abstract：:The temperature dependence of drug blockade of a calcium-dependent potassium channel K(Ca) has been studied in cultured CA1 hippocampal neurons. Channel openings from a 70-pS K+ channel were recorded when inside-out patches were exposed to a bath solution containing 140 mM K+ and 0.2 mM Ca2+. The mean open times of ch...

journal_title：Biophysical journal

authors： McLarnon JG,Wang XP

更新日期：1991-11-01 00:00:00