Binding features of diacetylmorphine (heroin) in whole blood and in blood fractions.

abstract：:Alamethicin and its analogs from cation selective, multi-conductance channels in lipid bilayers. The conductance levels have been thought to be due to a barrel-stave structure where conducting pores (barrels) are formed by the self-assembly of a variable number of alpha-helical rods (staves). The conductance transitio...

journal_title：Biophysical journal

authors： Laver DR

更新日期：1994-02-01 00:00:00

abstract：:Viral infection begins with the binding of a virus to a specific target on the surface of the host cell, followed by viral genome delivery into the host and a continuation of the infection process. Before binding occurs, the virus must first find its receptor by a process whose details are largely unknown. We applied ...

journal_title：Biophysical journal

authors： Rothenberg E,Sepúlveda LA,Skinner SO,Zeng L,Selvin PR,Golding I

更新日期：2011-06-22 00:00:00

abstract：:The Kir3.1/Kir3.4 channel is an inward rectifier, agonist-activated K(+) channel. The location of the binding site within the channel pore that coordinates polyamines (and is thus responsible for inward rectification) and the location of the gate that opens the channel in response to agonist activation is unclear. In ...

journal_title：Biophysical journal

authors： Makary SM,Claydon TW,Dibb KM,Boyett MR

更新日期：2006-06-01 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromat...

journal_title：Biophysical journal

authors： Tsuda M,Nelson B,Chang CH,Govindjee R,Ebrey TG

更新日期：1985-05-01 00:00:00

abstract：:Most, but not all, ion channels appear to obey the law of microscopic reversibility (or detailed balance). During the fitting of reaction mechanisms it is therefore often required that cycles in the mechanism should obey microscopic reversibility at all times. In complex reaction mechanisms, especially those that cont...

journal_title：Biophysical journal

authors： Colquhoun D,Dowsland KA,Beato M,Plested AJ

更新日期：2004-06-01 00:00:00

abstract：:Red fluorescent proteins are important tools in fluorescence-based life science research. Recently, we have introduced eqFP611, a red fluorescent protein with advantageous properties from the sea anemone Entacmaea quadricolor. Here, we have studied the submillisecond light-driven intramolecular dynamics between bright...

journal_title：Biophysical journal

authors： Schenk A,Ivanchenko S,Röcker C,Wiedenmann J,Nienhaus GU

更新日期：2004-01-01 00:00:00

abstract：:The Jun a 3 protein from mountain cedar (Juniperus ashei) pollen, a member of group 5 of the family of plant pathogenesis-related proteins (PR-proteins), reacts with serum IgE from patients with cedar hypersensitivity. We used the crystal structures of two other proteins of this group, thaumatin and an antifungal prot...

journal_title：Biophysical journal

authors： Soman KV,Midoro-Horiuti T,Ferreon JC,Goldblum RM,Brooks EG,Kurosky A,Braun W,Schein CH

更新日期：2000-09-01 00:00:00

abstract：:Calmodulin (CaM) expression is upregulated upon HIV-1 infection and interacts with proteins involved in viral processing, including the multifunctional HIV-1 MA protein. We present here the results of studies utilizing small-angle neutron scattering with contrast variation that, when considered in the light of earlier...

journal_title：Biophysical journal

authors： Taylor JE,Chow JYH,Jeffries CM,Kwan AH,Duff AP,Hamilton WA,Trewhella J

更新日期：2012-08-08 00:00:00

abstract：:Understanding the intermolecular interaction potential, V(r), of proteins under the influence of temperature, pressure, and salt concentration is essential for understanding protein aggregation, crystallization, and protein phase behavior in general. Here, we report small-angle x-ray scattering studies on dense lysozy...

journal_title：Biophysical journal

authors： Möller J,Schroer MA,Erlkamp M,Grobelny S,Paulus M,Tiemeyer S,Wirkert FJ,Tolan M,Winter R

更新日期：2012-06-06 00:00:00

abstract：:We present a molecular system where polymerization is controlled externally by tuning the elastic energy of the monomers. The elastic energy, provided by a DNA molecular spring, destabilizes the monomer state through a process analogous to domain swapping. This energy can be large (of approximately 10 kT) and thus dri...

journal_title：Biophysical journal

authors： Wang A,Zocchi G

更新日期：2009-03-18 00:00:00

abstract：:The motility of Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion...

journal_title：Biophysical journal

authors： Rogers SS,Waigh TA,Lu JR

更新日期：2008-04-15 00:00:00

abstract：:Aerotaxis is a particular form of "energy taxis". It is based on a largely elusive signal transduction machinery. In aerotaxis, oxygen dissolved in water plays the role of both attractant (at moderate concentrations) and repellent (at high and low concentrations). Cells swimming from favorable oxygen concentrations in...

journal_title：Biophysical journal

authors： Mazzag BC,Zhulin IB,Mogilner A

更新日期：2003-12-01 00:00:00

abstract：:The conformation of single laminin molecules adsorbed on synthetic substrates is directly observed making use of the phase magnitude in tapping mode atomic force microscopy (AFM). With AFM, it is not possible to differentiate the proteins on the substrate if use is made of the height signal, since the roughness of the...

journal_title：Biophysical journal

authors： Rodríguez Hernández JC,Salmerón Sánchez M,Soria JM,Gómez Ribelles JL,Monleón Pradas M

更新日期：2007-07-01 00:00:00

abstract：:Förster resonance energy transfer (FRET)-based biosensors for the quantitative analysis of intracellular signaling, including sensors for monitoring cyclic adenosine monophosphate (cAMP), are of increasing interest. The measurement of the donor/acceptor emission ratio in tandem biosensors excited at the donor excitati...

journal_title：Biophysical journal

authors： Salonikidis PS,Zeug A,Kobe F,Ponimaskin E,Richter DW

更新日期：2008-12-01 00:00:00

abstract：:Intracellular reactions are carried out in a crowded medium where the macromolecules occupy ∼40% of the total volume. This decrease in the available volume affects the activity of the reactants. Scaled particle theory is used for the estimation of the activity coefficients of the metabolites, and thereby for the asses...

journal_title：Biophysical journal

authors： Angeles-Martinez L,Theodoropoulos C

更新日期：2015-12-01 00:00:00

abstract：:Activation of skeletal muscle ryanodine receptors (RyRs) by suramin and disulfonic stilbene derivatives (Diisothiocyanostilbene-2',2'-disulfonic acid (DIDS), 4,4'-dibenzamidostilbene-2,2'-disulfonic acid (DBDS),and 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS)) was investigated using planar bilayers. One reversible...

journal_title：Biophysical journal

authors： O'Neill ER,Sakowska MM,Laver DR

更新日期：2003-03-01 00:00:00

abstract：:We propose a general scheme for measuring the attraction between mechanically frustrated semiflexible fibers by measuring their thermal fluctuations and shape. We apply this analysis to a system of sickle hemoglobin (HbS) fibers that laterally attract one another. These fibers appear to "zip" together before reaching ...

journal_title：Biophysical journal

authors： Jones CW,Wang JC,Briehl RW,Turner MS

更新日期：2005-04-01 00:00:00

abstract：:Recent biological optical spectroscopic studies have correlated discrete spectroscopic states with biological function in several systems. One of the challenges of molecular biophysics is to correlate structural changes with these spectroscopic states. From small-angle x-ray scattering one can obtain a key structural ...

journal_title：Biophysical journal

authors： Stamatoff J

更新日期：1979-05-01 00:00:00

abstract：:Ca2+ entry under resting conditions may be important for contraction of vascular smooth muscle, but little is known about the mechanisms involved. Ca2+ leakage was studied in the A7r5 smooth muscle-derived cell line by patch-clamp techniques. Two channels that could mediate calcium influx at resting membrane potential...

journal_title：Biophysical journal

authors： Obejero-Paz CA,Jones SW,Scarpa A

更新日期：1998-09-01 00:00:00

abstract：:Microstructured surfaces provide a unique framework to probe cell migration and cytoskeletal dynamics in a standardized manner. Here, we report on the steady-state occupancy probability of cells in asymmetric two-state microstructures that consist of two fibronectin-coated adhesion sites connected by a thin guidance c...

journal_title：Biophysical journal

authors： Fink A,Brückner DB,Schreiber C,Röttgermann PJF,Broedersz CP,Rädler JO

更新日期：2020-02-04 00:00:00

abstract：:We introduce an intrinsically multiplexed and easy to implement method to apply an external force to a biomolecule and thus probe its interaction with a second biomolecule or, more generally, its environment (for example, the cell membrane). We take advantage of the hydrodynamic interaction with a controlled fluid flo...

journal_title：Biophysical journal

authors： Türkcan S,Richly MU,Bouzigues CI,Allain JM,Alexandrou A

更新日期：2013-07-02 00:00:00

abstract：:The electrogenic transport of ATP and ADP by the mitochondrial ADP/ATP carrier (AAC) was investigated by recording transient currents with two different techniques for performing concentration jump experiments: 1) the fast fluid injection method: AAC-containing proteoliposomes were adsorbed to a solid supported membra...

journal_title：Biophysical journal

authors： Gropp T,Brustovetsky N,Klingenberg M,Müller V,Fendler K,Bamberg E

更新日期：1999-08-01 00:00:00

abstract：:We investigate lateral organization of lipid domains in vesicles versus supported membranes and monolayers. The lipid mixtures used are predominantly DOPC/DPPC/Chol and DOPC/BSM/Chol, which have been previously shown to produce coexisting liquid phases in vesicles and monolayers. In a monolayer at an air-water interfa...

journal_title：Biophysical journal

authors： Stottrup BL,Veatch SL,Keller SL

更新日期：2004-05-01 00:00:00

abstract：:The surprising observation that pressures of the order of 150 atmospheres can restore consciousness to an anesthetized animal has long been central to theories of the molecular mechanisms underlying general anesthesia. We have constructed a high-pressure gas chamber to test for "pressure reversal" of the best availabl...

journal_title：Biophysical journal

authors： Moss GW,Lieb WR,Franks NP

更新日期：1991-12-01 00:00:00

abstract：:We demonstrate a novel method for stretching a long DNA molecule in agarose gel with alternating current (AC) electric fields. The molecular motion of a long DNA (T4 DNA; 165.6 kb) in agarose gel was studied using fluorescence microscopy. The effects of a wide range of field frequencies, field strengths, and gel conce...

journal_title：Biophysical journal

authors： Kaji N,Ueda M,Baba Y

更新日期：2002-01-01 00:00:00

abstract：:Cholesterol is essential to the formation of phase-separated lipid domains in membranes. Lipid domains can exist in different thermodynamic phases depending on the molecular composition and play significant roles in determining structure and function of membrane proteins. We investigate the role of cholesterol in the ...

journal_title：Biophysical journal

authors： Pantelopulos GA,Straub JE

更新日期：2018-12-04 00:00:00

abstract：:The intracellular and interstitial potentials associated with each cell or fiber in multicellular preparations carrying a uniformly propagating wave are important for characterizing the electrophysiological behavior of the preparation and in particular, for evaluating the source contributed by each fiber. The aforemen...

journal_title：Biophysical journal

authors： Henriquez CS,Trayanova N,Plonsey R

更新日期：1988-06-01 00:00:00

abstract：:A dissipative system is approximated by a nonlinear rate equation: Z congruent to K1Z - K2Z3 (K2 greater than 0), in which the right side is derived from -delta G/delta Z of Taylor's series of the thermodynamic potential given by Gibbs' function G(Tc, Pc) (Z) at about the critical point C(Tc, Pc) of the control variab...

journal_title：Biophysical journal

authors： Shirane K,Tokimoto T,Yamaguchi Y

更新日期：1990-01-01 00:00:00

abstract：:An experimental method and analysis are introduced which provide direct quantitation of the strength of adhesive contact for large agglutinin-bonded regions between macroscopically smooth membrane capsules (e.g., red blood cells). The approach yields intrinsic properties for separation of adherent regions independent ...

journal_title：Biophysical journal

authors： Berk D,Evans E

更新日期：1991-04-01 00:00:00