Abstract:
:Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromatography we show that the chromophore of tR590 is primarily all-trans retinal and its conversion by light to tR370 causes the chromophore to isomerize primarily to the 13-cis conformation. Irradiation of the tR370 gives rise to a transient photoproduct absorbing at approximately 520 nm that decays back to the initial pigment tR590. In addition to all-trans retinal, the apomembrane of tR can also combine with 13-cis retinal but not with the 9- or 11-cis isomers.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Tsuda M,Nelson B,Chang CH,Govindjee R,Ebrey TGdoi
10.1016/S0006-3495(85)83969-2subject
Has Abstractpub_date
1985-05-01 00:00:00pages
721-4issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(85)83969-2journal_volume
47pub_type
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