Abstract:
:Tension and dynamic stiffness of passive rabbit psoas, rabbit semitendinosus, and waterbug indirect flight muscles were investigated to study the contribution of weak-binding cross-bridges and elastic filaments (titin and minititin) to the passive mechanical behavior of these muscles. Experimentally, a functional dissection of the relative contribution of actomyosin cross-bridges and titin and minititin was achieved by 1) comparing mechanically skinned muscle fibers before and after selective removal of actin filaments with a noncalcium-requiring gelsolin fragment (FX-45), and 2) studying passive tension and stiffness as a function of sarcomere length, ionic strength, temperature, and the inhibitory effect of a carboxyl-terminal fragment of smooth muscle caldesmon. Our data show that weak bridges exist in both rabbit skeletal muscle and insect flight muscle at physiological ionic strength and room temperature. In rabbit psoas fibers, weak bridge stiffness appears to vary with both thin-thick filament overlap and with the magnitude of passive tension. Plots of passive tension versus passive stiffness are multiphasic and strikingly similar for these three muscles of distinct sarcomere proportions and elastic proteins. The tension-stiffness plot appears to be a powerful tool in discerning changes in the mechanical behavior of the elastic filaments. The stress-strain and stiffness-strain curves of all three muscles can be merged into one, by normalizing strain rate and strain amplitude of the extensible segment of titin and minititin, further supporting the segmental extension model of resting tension development.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Granzier HL,Wang Kdoi
10.1016/S0006-3495(93)81262-1subject
Has Abstractpub_date
1993-11-01 00:00:00pages
2141-59issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(93)81262-1journal_volume
65pub_type
杂志文章abstract::We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2010.11.013
更新日期:2010-12-15 00:00:00
abstract::Forces applied by intact mucus-propelling cilia were measured for the first time that we know of using a combined atomic force microscopy (AFM) and electrooptic system. The AFM probe was dipped into a field of beating cilia and its time-dependent deflection was recorded as it was struck by the cilia while the electroo...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.094698
更新日期:2007-03-01 00:00:00
abstract::Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.105.075614
更新日期:2006-05-15 00:00:00
abstract::We present a quantitative analysis of conformational changes of the nucleotide-binding subunits, MalK(2), of the maltose ATP-binding cassette importer MalFGK(2) during the transport cycle. Distance changes occurring between selected residues were monitored in the full transporter by site-directed spin-labeling electro...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.108.132456
更新日期:2008-09-15 00:00:00
abstract::Understanding protein phase behavior is important for purification, storage, and stable formulation of protein drugs in the biopharmaceutical industry. Glycoproteins, such as monoclonal antibodies (MAbs) are the most abundant biopharmaceuticals and probably the most difficult to crystallize among water-soluble protein...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.098293
更新日期:2007-07-15 00:00:00
abstract::Mössbauer spectra of anhydrohemoglobin establish the existence of two ferrous iron spin states in anhydrohemoglobin. Magnetic susceptibility measurements show that anhydrohemoglobin is paramagnetic and has an effective magnetic moment per iron atom of 3.5+/-0.1mu(B) at low temperatures. In conjunction with the suscept...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(67)86614-1
更新日期:1967-11-01 00:00:00
abstract::Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2018.08.046
更新日期:2018-10-16 00:00:00
abstract::Structures of truncated versions of the influenza A virus M2 proton channel have been determined recently by x-ray crystallography in the open conformation of the channel, and by NMR in the closed state. The structures differ in the position of the bound inhibitors. The x-ray structure shows a single amantadine molecu...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.09.004
更新日期:2009-11-18 00:00:00
abstract::Conditions for which a ligand reversibly bound to a cell surface dissociates and then rebinds to the surface have been theoretically examined. The coupled differential equations that describe reaction at the interface between sites on a plane and three-dimensional solution have been described previously (Thompson, N. ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)77836-1
更新日期:1998-03-01 00:00:00
abstract::The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2018.11.3141
更新日期:2019-02-05 00:00:00
abstract::A refined mesoscopic model for the cumulative effect of repulsive excluded volume protein-protein interaction and attractive protein-surface interaction upon the properties of a trace protein capable of fiber formation is presented. The model predicts that very small changes in the magnitude of bulk volume occupancy o...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2019.09.033
更新日期:2019-11-05 00:00:00
abstract::Molecular dynamics simulations were performed to describe the function of the ion-channel-forming toxin α-hemolysin (αHL) in lipid membranes that were composed of either 1,2-diphytanoyl-sn-glycero-3-phospho-choline or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-choline. The simulations highlight the importance of lipid ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2018.09.009
更新日期:2018-11-06 00:00:00
abstract::Saturation transfer electron paramagnetic resonance (ST-EPR) spectroscopy has been employed to characterize the very slow microsecond to millisecond rotational dynamics of a wide range of nitroxide spin-labeled proteins and other macromolecules in the past three decades. The vast majority of this previous work has bee...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.103.035048
更新日期:2004-06-01 00:00:00
abstract::EXACT FORMULAS ARE DERIVED FOR THE ENERGY CHANGE OF A DIPOLE SYSTEM WITH TWO ENERGY STATES (OR BANDS) IN A CHANGING FIELD IN TWO CASES: (a) no dipole flip-flop and (b) dipole flip-flop caused by stimulation. Based on these formulas, the positive and negative heats are calculated. The results are in good agreement with...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(72)86152-6
更新日期:1972-09-01 00:00:00
abstract::Recent advances in imaging technology have revealed oscillations of cyclic adenosine monophosphate (cAMP) in insulin-secreting cells. These oscillations may be in phase with cytosolic calcium oscillations or out of phase. cAMP oscillations have previously been modeled as driven by oscillations in calcium, based on the...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2015.06.024
更新日期:2015-07-21 00:00:00
abstract::Limited proteolysis of RNase-A yields a short N-terminal S-peptide segment and the larger S-protein. Binding of S-peptide to S-protein results in the formation of an enzymatically active RNase-S protein. S-peptide undergoes a transition from intrinsic disorder to an ordered helical state upon association with S-protei...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2017.08.008
更新日期:2017-10-03 00:00:00
abstract::To record post synaptic potentials or electrical activity from processes of single cells in a central nervous system (CNS) preparation in situ, voltage sensitive dyes can be injected intracellularly, thereby staining only the cell under investigation. We report the structure, evaluation, and synthesis of 11 fluorescen...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(87)83389-1
更新日期:1987-04-01 00:00:00
abstract::Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress condition...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.120717
更新日期:2008-04-01 00:00:00
abstract::The reported rates of thermal 13-cis to all-trans isomerization of the protonated Schiff base of retinal (PSBR) in solution and in bacteriorhodopsin (BR) are shown to be correlated with the red shift in the absorption maximum of the chromophore, though the linear fit is different for BR and for a model PSBR in solutio...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(91)82070-7
更新日期:1991-08-01 00:00:00
abstract::Understanding exposure thresholds for the response of biological systems to extremely low frequency (ELF) electric and magnetic fields is a fundamental problem of long-standing interest. We consider a two-state model for voltage-gated channels in the membrane of an isolated elongated cell (Lcell = 1 mm; rcell = 25 mic...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)77669-6
更新日期:1998-11-01 00:00:00
abstract::Diffusional encounters between a dumbbell model of a cleft enzyme and a dumbbell model of an elongated ligand are simulated by Brownian dynamics. The simulations take into account electrostatic and hydrodynamic interactions between the molecules. It is shown that the primary effect of inclusion of hydrodynamic interac...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(95)79874-5
更新日期:1995-07-01 00:00:00
abstract::Chlamydomonas exhibits a sequence of a photoreceptor current and two flagellar currents upon stimulation with bright green flashes. The currents are thought to be a prerequisite for the well-known photophobic responses. In the preceding paper, we analyzed the kinetics of these currents and their dependence on extracel...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79636-4
更新日期:1996-02-01 00:00:00
abstract::In the studies of force-induced conformational transitions of biomolecules, the large timescale difference from experiments presents the challenge of obtaining convergent sampling for molecular dynamics simulations. To circumvent this fundamental problem, an approach combining the replica-exchange method and umbrella ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.11.004
更新日期:2010-02-17 00:00:00
abstract::The Ca(2+) permeability of N-methyl-D-aspartate receptor (NMDA-R) channels was studied in human embryonic kidney cells transfected with the NR1-NR2A subunit combination. To determine the fractional Ca(2+) current (P(f)), measurements of fura-2-based Ca(2+) influx and whole-cell currents were made in symmetrical monova...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79782-5
更新日期:1996-05-01 00:00:00
abstract::The proposition is made that the red cell membrane is a two-dimensional, incompressible material and a general stress-strain law is developed for finite deformations. In the linear form, the character of such a material is analogous to a two-dimensional Mooney material (e.g., rubber), indicating that the molecular str...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(73)86035-7
更新日期:1973-09-01 00:00:00
abstract::Using a method they developed, Stamatoff and Krimm (1976) have phased swelling data from nerve myelin. Although most phases agree with those I determined previously, there are a few differences. In this letter the two different phasings, theirs and my own, are used to compute the corresponding electron-density profile...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(79)85242-X
更新日期:1979-04-01 00:00:00
abstract::Nucleoprotein filament formation by recombinases is central to homologous recombination. To follow this process, we used fluorescent human Rad51 recombinase to visualize the interactions with double-stranded DNA (dsDNA). Fluorescence imaging revealed that Rad51 filament formation on dsDNA initiates from multiple nucle...
journal_title:Biophysical journal
pub_type: 信件
doi:10.1529/biophysj.106.089466
更新日期:2006-10-15 00:00:00
abstract::A recent thermodynamic model describes a reversible reaction between cholesterol (C) and phospholipid (P) to form a condensed complex C(nq)P(np). Here q and p are relatively prime integers used to define the stoichiometric composition, and n is a measure of cooperativity. The present study applies this model to the sc...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)75920-6
更新日期:2001-11-01 00:00:00
abstract::O-acetylserine sulfhydrylase, a homo-dimeric enzyme from Salmonella typhimurium, covalently binds one pyridoxal 5'-phosphate molecule per subunit as a fluorescent coenzyme. Different tautomers of the Schiff base between the coenzyme and lysine 41 generate structured absorption and fluorescence spectra upon one-photon ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)76167-X
更新日期:2001-04-01 00:00:00
abstract::A 15-ns molecular dynamics (MD) simulation of the fully hydrated dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer in the liquid-crystalline state was carried out to investigate the effect of Chol on the hydrocarbon chain region of the bilayer. The last 8-ns fragment of the generated trajectory was used f...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)75867-5
更新日期:2001-10-01 00:00:00