Modeling of glucose-induced cAMP oscillations in pancreatic β cells: cAMP rocks when metabolism rolls.

abstract：:Inositol 1,4,5-trisphosphate (InsP3) acts on intracellular receptors to cause liberation of Ca2+ ions into the cytosol as repetitive spikes and propagating waves. We studied the processes underlying this regenerative release of Ca2+ by monitoring with high resolution the kinetics of Ca2+ flux evoked in Xenopus oocytes...

journal_title：Biophysical journal

authors： Parker I,Yao Y,Ilyin V

更新日期：1996-01-01 00:00:00

abstract：:The influence of mass transport on ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor, was investigated. A one-dimensional computer model for the mass transport of ligand between the bulk solution and the polymer gel and within the gel was employed, and the influe...

journal_title：Biophysical journal

authors： Schuck P

更新日期：1996-03-01 00:00:00

abstract：:The mouse major urinary protein (MUP) has proved to be an intriguing test bed for detailed studies on protein-ligand recognition. NMR, calorimetric, and modeling investigations have revealed that the thermodynamics of ligand binding involve a complex interplay between competing enthalpic and entropic terms. We perform...

journal_title：Biophysical journal

authors： Roy J,Laughton CA

更新日期：2010-07-07 00:00:00

abstract：:Experiments have shown that the ability of the HIV-1 virus to infect cells can be greatly diminished by deactivation of the N-terminal (fusion) peptide of its glycoprotein gp41. Deactivation can be achieved by the deletion of several amino acid residues, or replacement of a hydrophobic residue with a polar residue, to...

journal_title：Biophysical journal

authors： Maddox MW,Longo ML

更新日期：2002-12-01 00:00:00

abstract：:A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonl...

journal_title：Biophysical journal

authors： Gratton E,Limkeman M

更新日期：1983-12-01 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the la...

journal_title：Biophysical journal

authors： Vulin C,Di Meglio JM,Lindner AB,Daerr A,Murray A,Hersen P

更新日期：2014-05-20 00:00:00

abstract：:Deuterium NMR spectroscopy was used to study how the positioning of a dimerization motif within a transbilayer polypeptide influences its orientation and dynamics in bilayers. Three polypeptide variants comprising glycophorin A transmembrane (GpATM) dimerization motifs incorporated into lysine-terminated poly-leucine-...

journal_title：Biophysical journal

authors： McDonald MC,Booth V,Morrow MR

更新日期：2011-02-02 00:00:00

abstract：:We used micron-sized latex spheres to probe the phase state and the viscoelastic properties of dimyristoylphosphatidylcholine (DMPC) bilayers as a function of temperature. One or two particles were manipulated and stuck to a DMPC giant vesicle by means of an optical trap. Above the fluid-gel main transition temperatur...

journal_title：Biophysical journal

authors： Dimova R,Pouligny B,Dietrich C

更新日期：2000-07-01 00:00:00

abstract：:Hemolymph and protein-free hemolymph obtained from Loligo pealei were analyzed for cation concentration by the method of atomic absorption spectroscopy, for the following ions: Na+, K+, Ca++, and Mg++. No significant differences were seen in ion concentration between hemolymph and protein-free hemolymph. Of particular...

journal_title：Biophysical journal

authors： Shoukimas J,Adelman WJ Jr,Sege V

更新日期：1977-05-01 00:00:00

abstract：:Thin filament regulation of contraction is thought to involve the binding of two activating ligands: Ca2+ and strongly bound cross-bridges. The specific cross-bridge states required to promote thin filament activation have not been identified. This study examines the relationship between cross-bridge cycling and thin ...

journal_title：Biophysical journal

authors： Wahr PA,Metzger JM

更新日期：1999-04-01 00:00:00

abstract：:Stabilized intermediate redox states of cytochrome c are generated by radiolytic reduction of initially oxidized enzyme in glass matrices at liquid nitrogen temperature. In the intermediate states the heme group is reduced by hydrated electrons, whereas the protein conformation is restrained close to its oxidized form...

journal_title：Biophysical journal

authors： Cartling B

更新日期：1983-08-01 00:00:00

abstract：:The enzyme F1-adenosine triphosphatase (ATPase) is a molecular motor that converts the chemical energy stored in the molecule adenosine triphosphate (ATP) into mechanical rotation of its gamma-subunit. During steady-state catalysis, the three catalytic sites of F1 operate in a cooperative fashion such that at every in...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2004-11-01 00:00:00

abstract：:The structural basis of the wide variability of the physical properties of fibrin clots and the process of assembly of the clot were investigated by electron microscopy of fibers formed under various ionic conditions. In addition, highly specific proteolytic enzymes from different snake venoms were used to remove sele...

journal_title：Biophysical journal

authors： Weisel JW

更新日期：1986-12-01 00:00:00

abstract：:Trains of nerve impulses from the crayfish stretch receptor under steady conditions are found to be extremely regular with a standard deviation of S = 0.7 x 10(-2)tau when tau, the mean interval, is in the 15 to 80 msec range. Such a small fluctuation increases the problems of film recording, accurate measurement of i...

journal_title：Biophysical journal

authors： Firth DR

更新日期：1966-03-01 00:00:00

abstract：:Investigations into the spatiotemporal dynamics of DNA repair using live-cell imaging are aided by the ability to generate well defined regions of ultravioletlike photolesions in an optical microscope. We demonstrate that multiphoton excitation of DNA in live cells with visible femtosecond pulses produces thymine cycl...

journal_title：Biophysical journal

authors： Daddysman MK,Fecko CJ

更新日期：2011-11-02 00:00:00

abstract：:The interaction of DNA with the polyamine spermine(4+) (Spm(4+)), sodium ions, and water molecules has been studied using molecular dynamics computer simulations in a system modeling a DNA crystal. The simulation model consisted of three B-DNA decamers in a periodic hexagonal cell, containing 1200 water molecules, 8 S...

journal_title：Biophysical journal

authors： Korolev N,Lyubartsev AP,Laaksonen A,Nordenskiöld L

更新日期：2002-06-01 00:00:00

abstract：:T cell receptor phosphorylation by Lck is an essential step in T cell activation. It is known that the conformational states of Lck control enzymatic activity; however, the underlying principles of how Lck finds its substrate over the plasma membrane remain elusive. Here, single-particle tracking is paired with photoa...

journal_title：Biophysical journal

authors： Hilzenrat G,Pandžić E,Yang Z,Nieves DJ,Goyette J,Rossy J,Ma Y,Gaus K

更新日期：2020-03-24 00:00:00

abstract：:The structural dynamics of bovine erythrocyte Cu, Zn superoxide dismutase (BSOD) was studied by time-resolved fluorescence spectroscopy. BSOD is a homodimer containing a single tyrosine residue (and no tryptophan) per subunit. Frequency-domain fluorometry revealed a heterogeneous fluorescence decay that could be descr...

journal_title：Biophysical journal

authors： Ferreira ST,Stella L,Gratton E

更新日期：1994-04-01 00:00:00

abstract：:High-affinity and cooperative binding of two Ca(2+) per ATPase (SERCA) occurs within the membrane-bound region of the enzyme. Direct measurements of binding at various Ca(2+) concentrations demonstrate that site-directed mutations within this region interfere selectively with Ca(2+) occupancy of either one or both bin...

journal_title：Biophysical journal

authors： Inesi G,Zhang Z,Lewis D

更新日期：2002-11-01 00:00:00

abstract：:Root mean-square deviation (RMSD) after roto-translational least-squares fitting is a measure of global structural similarity of macromolecules used commonly. On the other hand, experimental x-ray B-factors are used frequently to study local structural heterogeneity and dynamics in macromolecules by providing direct i...

journal_title：Biophysical journal

authors： Kuzmanic A,Zagrovic B

更新日期：2010-03-03 00:00:00

abstract：:When cells migrate through constricting pores, they incur DNA damage and develop genomic variation. Experiments show that this damage is not due to DNA breakage from mechanical stress on chromatin in the deformed nucleus. Here we propose a model for a mechanism by which nuclear deformation can lead to DNA damage. We t...

journal_title：Biophysical journal

authors： Bennett RR,Pfeifer CR,Irianto J,Xia Y,Discher DE,Liu AJ

更新日期：2017-06-06 00:00:00

abstract：:Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models are constructed computationally by building specific regions of the amino acid sequence as alpha helices and then positio...

journal_title：Biophysical journal

authors： Trewhella J,Anderson S,Fox R,Gogol E,Khan S,Engelman D,Zaccai G

更新日期：1983-06-01 00:00:00

abstract：:Structural rearrangements of the myosin upper-50 kD subdomain are thought to play a key role in coordinating actin binding with nucleotide hydrolysis during the myosin ATPase cycle. Such rearrangements could open and close the active site in opposition to the actin-binding cleft, helping explain the opposing affinitie...

journal_title：Biophysical journal

authors： Robertson CI,Gaffney DP 2nd,Chrin LR,Berger CL

更新日期：2005-09-01 00:00:00

abstract：:In skeletal muscle, excitation-contraction coupling involves the activation of dihydropyridine receptors (DHPR) and type-1 ryanodine receptors (RyR1) to produce depolarization-dependent sarcoplasmic reticulum Ca²⁺ release via orthograde signaling. Another form of DHPR-RyR1 communication is retrograde signaling, in whi...

journal_title：Biophysical journal

authors： Olojo RO,Hernández-Ochoa EO,Ikemoto N,Schneider MF

更新日期：2011-05-18 00:00:00

abstract：:The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive i...

journal_title：Biophysical journal

authors： TAYLOR EW,CRAMER W

更新日期：1963-03-01 00:00:00

abstract：:By employing classical molecular dynamics, correlation analysis of coupling between slow and fast dynamical modes, and free energy (umbrella) sampling using classical as well as mixed quantum mechanics molecular mechanics force fields, we uncover a possible pathway for phosphoryl transfer in the self-cleaving reaction...

journal_title：Biophysical journal

authors： Radhakrishnan R

更新日期：2007-10-01 00:00:00

abstract：:In an open circuit there can be no net cation flux through membranes containing only cation-selective channels, because electroneutrality must be maintained. If the channels are so narrow that water and cations cannot pass by each other, then the net water flux through those "single-file" channels that contain a catio...

journal_title：Biophysical journal

authors： Dani JA,Levitt DG

更新日期：1981-08-01 00:00:00

abstract：:A mode of internal motion of single tryptophan, Trp 86, of Streptomyces subtilisin inhibitor, was analyzed from its time-resolved fluorescence. The intensity and anisotropy decays of Trp 86 were measured in the picosecond range. These decays were analyzed with theoretical expressions derived assuming that the indole r...

journal_title：Biophysical journal

authors： Tanaka F,Tamai N,Mataga N,Tonomura B,Hiromi K

更新日期：1994-08-01 00:00:00

abstract：:Calmodulin (CaM) is an intracellular cooperative calcium-binding protein essential for activating many diverse target proteins. Biophysical studies of the calcium-induced conformational changes of CaM disagree on the structure of the linker between domains and possible orientations of the domains. Molecular dynamics s...

journal_title：Biophysical journal

authors： Sorensen BR,Shea MA

更新日期：1996-12-01 00:00:00