A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution.

Abstract:

:A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonlinear dynode characteristic are discussed in terms of the harmonic content of the detected signal. A source of systematic errors due to nonhomogeneous modulation is also discussed with particular attention to the color effect of the photomultipliers. The application of the phase fluorometer to the measurement of very long and very short lifetimes is reported. Some application to the measurement of multiexponential decays is also illustrated.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Gratton E,Limkeman M

doi

10.1016/S0006-3495(83)84305-7

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

315-24

issue

3

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(83)84305-7

journal_volume

44

pub_type

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