Cholesterol effects on the phosphatidylcholine bilayer nonpolar region: a molecular simulation study.

abstract：:This paper presents a study of the influence of normalization errors on size distributions obtained from the analysis of intensity fluctuations by photon correlation spectroscopy. The effects of these errors are demonstrated by means of computer-generated autocorrelation functions simulating light scattered from a mon...

journal_title：Biophysical journal

authors： Ruf H

更新日期：1989-07-01 00:00:00

abstract：:We performed pressure-tuning hole-burning experiments on a modified cytochrome c protein in a glycerol/buffer glass. The shift and the broadening of the holes were investigated for various frequencies within the inhomogeneous band. On the basis of a simple model, we were able to estimate the interaction range between ...

journal_title：Biophysical journal

authors： Lesch H,Schlichter J,Friedrich J,Vanderkooi JM

更新日期：2004-01-01 00:00:00

abstract：:The conformation of single laminin molecules adsorbed on synthetic substrates is directly observed making use of the phase magnitude in tapping mode atomic force microscopy (AFM). With AFM, it is not possible to differentiate the proteins on the substrate if use is made of the height signal, since the roughness of the...

journal_title：Biophysical journal

authors： Rodríguez Hernández JC,Salmerón Sánchez M,Soria JM,Gómez Ribelles JL,Monleón Pradas M

更新日期：2007-07-01 00:00:00

abstract：:We present a new approach to probing single-particle dynamics that uses dynamic light scattering from a localized region. By scattering a focused laser beam from a micron-size particle, we measure its spatial fluctuations via the temporal autocorrelation of the scattered intensity. We demonstrate the applicability of ...

journal_title：Biophysical journal

authors： Meller A,Bar-Ziv R,Tlusty T,Moses E,Stavans J,Safran SA

更新日期：1998-03-01 00:00:00

abstract：:In its most restrictive interpretation, the notion of chromatin remodeling refers to the action of chromatin-remodeling enzymes on nucleosomes with the aim of displacing and removing them from the chromatin fiber (the effective polymer formed by a DNA molecule and proteins). This local modification of the fiber struct...

journal_title：Biophysical journal

authors： Blossey R,Schiessel H

更新日期：2018-05-22 00:00:00

abstract：:The freshwater polyp Hydra can regenerate from tissue fragments or random cell aggregates. We show that the axis-defining step ("symmetry breaking") of regeneration requires mechanical inflation-collapse oscillations of the initial cell ball. We present experimental evidence that axis definition is retarded if these o...

journal_title：Biophysical journal

authors： Soriano J,Rüdiger S,Pullarkat P,Ott A

更新日期：2009-02-18 00:00:00

abstract：:Single-stranded junctions/loops are frequently occurring structural motifs in nucleic acid structures. Due to the polyanionic nature of the nucleic acid backbone, metal ions play a crucial role in the loop stability. Here we use the tightly bound ion theory, which can account for the possible ion correlation and ensem...

journal_title：Biophysical journal

authors： Tan ZJ,Chen SJ

更新日期：2008-07-01 00:00:00

abstract：:We are interested in modeling the membrane-spanning domain of the serotonin 5-HT1A G-protein coupled receptor. This superfamily of proteins is predicted to share the topology of the seven transmembrane helices of bacteriorhodopsin (BR), even though no significant sequence homology had been identified. We found signifi...

journal_title：Biophysical journal

authors： Ballesteros JA,Weinstein H

更新日期：1992-04-01 00:00:00

abstract：:The continuum and single jump treatments of ion transport through black lipid membranes predict experimentally distinguishable results, even when the same mechanistic assumptions are made and the same potential-distance profile is used. On the basis of steady-state current-voltage curves for nonactin-mediated transpor...

journal_title：Biophysical journal

authors： van Dijk C,de Levie R

更新日期：1985-07-01 00:00:00

abstract：:A challenge in the understanding of creatine kinase (CK) fluxes reflected by NMR magnetization transfer in the perfused rat heart is the choice of a kinetic model of analysis. The complexity of the energetic pathways, due to the presence of adenosine triphosphate (ATP)-inorganic phosphate (Pi) exchange, of metabolite ...

journal_title：Biophysical journal

authors： Joubert F,Hoerter JA,Mazet JL

更新日期：2001-12-01 00:00:00

abstract：:Deuterium nuclear magnetic resonance was used to monitor lipid acyl-chain orientational order in suspensions of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) containing Ca(2+) and the lung surfactant proteins SP-A and SP-B separately and together. To distinguish between protein-lipid...

journal_title：Biophysical journal

authors： Morrow MR,Temple S,Stewart J,Keough KM

更新日期：2007-07-01 00:00:00

abstract：:Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interr...

journal_title：Biophysical journal

authors： Otto MR,Lillo MP,Beechem JM

更新日期：1994-12-01 00:00:00

abstract：:Atomic force microscopy (AFM) is nowadays frequently applied to determine interaction forces between biological molecules. Starting with the detection of the first discrete unbinding forces between ligands and receptors by AFM only several years ago, measurements have become more and more quantitative. At the same tim...

journal_title：Biophysical journal

authors： Willemsen OH,Snel MM,Cambi A,Greve J,De Grooth BG,Figdor CG

更新日期：2000-12-01 00:00:00

abstract：:A recent theory for the long time dynamics of flexible chain molecules is applied for the first time to a peptide of biological importance, the neurotransmitter met-enkephalin. The dynamics of met-enkephalin is considerably more complicated than that of the previously studied glycine oligomers; met-enkephalin contains...

journal_title：Biophysical journal

authors： Kostov KS,Freed KF

更新日期：1999-01-01 00:00:00

abstract：:Sarcomere lengths, cell widths, indices of stiffness, and striation pattern uniformity were determined from radially compressed isolated adult cardiac myocytes from the rat. Single cells were bathed in a series of relaxing solutions containing 0-15% concentrations of nonpenetrating long chain polymers PVP-40 and dextr...

journal_title：Biophysical journal

authors： Roos KP,Brady AJ

更新日期：1990-11-01 00:00:00

abstract：:The mechanism for transport of divalent cations across phospholipid bilayers by the ionophore A23187 was investigated. The intrinsic fluorescence of the ionophore was used in equilibrium and rapid-mixing experiments as an indicator of ionophore environment and complexation with divalent cations. The neutral (protonate...

journal_title：Biophysical journal

authors： Kolber MA,Haynes DH

更新日期：1981-11-01 00:00:00

abstract：:The Mie theory of scattering is used to provide new information on how changes in particle volume, with no change in dry weight, should influence light scattering for various scattering angles and particle sizes. Many biological cells (e.g., algal cells, erythrocytes) and large subcellular structures (e.g., chloroplas...

journal_title：Biophysical journal

authors： Latimer P,Pyle BE

更新日期：1972-07-01 00:00:00

abstract：:We investigate the conditions under which nonideality in solution influences the Donnan equilibrium. Of the various parameters that characterize this equilibrium, the osmotic pressure established across the Donnan membrane is found to be particularly sensitive to intermolecular interactions between the diffusible and ...

journal_title：Biophysical journal

authors： Stell G,Joslin CG

更新日期：1986-11-01 00:00:00

abstract：:Sickle hemoglobin forms long, multistranded polymers that account for the pathophysiology of the disease. The molecules in these polymers make significant contacts along the polymer axis (i.e., axial contacts) as well as making diagonally directed contacts (i.e., lateral contacts). The axial contacts do not engage the...

journal_title：Biophysical journal

authors： Wang Y,Ferrone FA

更新日期：2013-11-05 00:00:00

abstract：:Unraveling the conformation of membrane-bound viral fusion peptides is essential for understanding how those peptides destabilize the bilayer topology of lipids that is important for virus-cell membrane fusion. Here, molecular dynamics (MD) simulations were performed to investigate the conformation of the 20 amino aci...

journal_title：Biophysical journal

authors： Huang Q,Chen CL,Herrmann A

更新日期：2004-07-01 00:00:00

abstract：:The interaction between actin and myosin in the filament array of glycerinated muscle fibers has been monitored using paramagnetic probes and mechanical measurements. Both fiber stiffness and the spectra of probes bound to a reactive sulfydral on the myosin head were measured as the actomyosin bond was weakened by add...

journal_title：Biophysical journal

authors： Pate E,Cooke R

更新日期：1988-04-01 00:00:00

abstract：:The effect of a transmembrane peptide on the domain structure of a two-component, two-phase lipid bilayer composed of dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) was examined by spin label electron spin resonance (ESR) spectroscopy. The peptide, pOmpA, is the hydrophobic, 25-residu...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Gierasch LM,Thompson TE

更新日期：1994-06-01 00:00:00

abstract：:We report detection and quantification of ultraviolet (UV) damage in DNA at a single molecule level by atomic force microscopy (AFM). By combining the supercoiled plasmid relaxation assay with AFM imaging, we find that high doses of medium wave ultraviolet (UVB) and short wave ultraviolet (UVC) light not only produce ...

journal_title：Biophysical journal

authors： Jiang Y,Ke C,Mieczkowski PA,Marszalek PE

更新日期：2007-09-01 00:00:00

abstract：:Deuterium magnetic resonance is applied to the study of the liquid crystalline and gel phases, and of the phase transition, of a multilamellar dispersion of chain perdeuterated (d62)-dipalmitoyl phosphatidylcholine/H2O. Analysis of the deuterium spectra in terms of the moments of the spectra allows one to make quantit...

journal_title：Biophysical journal

authors： Davis JH

更新日期：1979-09-01 00:00:00

abstract：:The cell membrane of Salinibacter ruber contains xanthorhodopsin, a light-driven transmembrane proton pump with two chromophores: a retinal and the carotenoid, salinixanthin. Action spectra for transport had indicated that light absorbed by either is utilized for function. If the carotenoid is an antenna in this prote...

journal_title：Biophysical journal

authors： Balashov SP,Imasheva ES,Wang JM,Lanyi JK

更新日期：2008-09-01 00:00:00

abstract：:Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golg...

journal_title：Biophysical journal

authors： Li J,Barylko B,Eichorst JP,Mueller JD,Albanesi JP,Chen Y

更新日期：2016-08-09 00:00:00

abstract：:Stationary and kinetic experiments were performed on lipid bilayer membranes to study the mechanism of iodine- and bromine-mediated halide transport in detail. The stationary conductance data suggested that four different 1:1 complexes between I2 and Br2 and the halides I- and Br- were responsible for the observed con...

journal_title：Biophysical journal

authors： Klotz KH,Benz R

更新日期：1993-12-01 00:00:00

abstract：:Intracellular Ca2+ was determined with the fura-2 technique during electrically induced contractions in the rabbit rectococcygeus smooth muscle at 22 degreesC. The muscles were electrically activated to give short, reproducible contractions. Intracellular [Ca2+] increased during activation; the increase in [Ca2+] prec...

journal_title：Biophysical journal

authors： Arner A,Malmqvist U,Rigler R

更新日期：1998-10-01 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:The axial deformation of a pipette-pressurized fluid membrane bag produces minuscule yet well-defined, reproducible forces. The stiffness of this ultrasensitive force transducer is tunable and largely independent of the constitutive membrane behavior. Based on a rigorous variational treatment, we present both numerica...

journal_title：Biophysical journal

authors： Heinrich V,Ounkomol C

更新日期：2007-07-15 00:00:00