Excitation energy-transfer and the relative orientation of retinal and carotenoid in xanthorhodopsin.

abstract：:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily...

journal_title：Biophysical journal

authors： Casuso I,Sens P,Rico F,Scheuring S

更新日期：2010-10-06 00:00:00

abstract：:The domains of the giant muscle protein titin (connectin) provide interaction sites for other sarcomeric proteins and fulfill mechanical functions. In this paper we compare the unfolding forces of defined regions of different titin isoforms by single-molecule force spectroscopy. Constructs comprising six to eight immu...

journal_title：Biophysical journal

authors： Rief M,Gautel M,Schemmel A,Gaub HE

更新日期：1998-12-01 00:00:00

abstract：:The functions and elasticities of the cell are largely related to the structures of the cytoskeletons underlying the lipid bilayer. Among various cell types, the red blood cell (RBC) possesses a relatively simple cytoskeletal structure. Underneath the membrane, the RBC cytoskeleton takes the form of a two-dimensional ...

journal_title：Biophysical journal

authors： Lai L,Xu X,Lim CT,Cao J

更新日期：2015-12-01 00:00:00

abstract：:Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromat...

journal_title：Biophysical journal

authors： Tsuda M,Nelson B,Chang CH,Govindjee R,Ebrey TG

更新日期：1985-05-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bu...

journal_title：Biophysical journal

authors： Kaufman EN,Jain RK

更新日期：1991-09-01 00:00:00

abstract：:The hydrophobic surfactant proteins, SP-B and SP-C, greatly accelerate the adsorption of the surfactant lipids to an air/water interface. Previous studies of factors that affect curvature suggest that vesicles may adsorb via a rate-limiting structure with prominent negative curvature, in which the hydrophilic face of ...

journal_title：Biophysical journal

authors： Chavarha M,Loney RW,Rananavare SB,Hall SB

更新日期：2013-02-05 00:00:00

abstract：:Unraveling the conformation of membrane-bound viral fusion peptides is essential for understanding how those peptides destabilize the bilayer topology of lipids that is important for virus-cell membrane fusion. Here, molecular dynamics (MD) simulations were performed to investigate the conformation of the 20 amino aci...

journal_title：Biophysical journal

authors： Huang Q,Chen CL,Herrmann A

更新日期：2004-07-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study molecular dynamics inside living cells. During the past years, several laboratories have used FRAP to image the motion of RNA-protein and other macromolecular complexes in the nucleus and cytoplasm. In the case of mRNAs, there is growin...

journal_title：Biophysical journal

authors： Braga J,McNally JG,Carmo-Fonseca M

更新日期：2007-04-15 00:00:00

abstract：:Guanine-rich DNA repeat sequences located at the terminal ends of chromosomal DNA can fold in a sequence-dependent manner into G-quadruplex structures, notably the terminal 150-200 nucleotides at the 3' end, which occur as a single-stranded DNA overhang. The crystal structures of quadruplexes with two and four human t...

journal_title：Biophysical journal

authors： Haider S,Parkinson GN,Neidle S

更新日期：2008-07-01 00:00:00

abstract：:Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by dete...

journal_title：Biophysical journal

authors： Carattino A,Keizer VIP,Schaaf MJM,Orrit M

更新日期：2016-12-06 00:00:00

abstract：:Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates ...

journal_title：Biophysical journal

authors： Hu J,Asbury T,Achuthan S,Li C,Bertram R,Quine JR,Fu R,Cross TA

更新日期：2007-06-15 00:00:00

abstract：:The human brain may be regarded as an irreversible system which is constrained by a fixed inflow of free energy in the form of chemical nourishment from within the body and information from the environment. The evolution of the internal spontaneous process may be described as a path on the saddle surface of entropy pr...

journal_title：Biophysical journal

authors： Kirkaldy JS

更新日期：1965-11-01 00:00:00

abstract：:The polar interface of membranes containing phosphatidylglycerol or cholesterol was studied by (2)H nuclear magnetic resonance (NMR) as a function of membrane hydration. The membranes were macroscopically aligned and hydrated with deuterium oxide. Water uptake and membrane annealing was achieved under NMR control, usi...

journal_title：Biophysical journal

authors： Kurze V,Steinbauer B,Huber T,Beyer K

更新日期：2000-05-01 00:00:00

abstract：:The mechanism of the "ground permeability" of the human erythrocyte membrane for K+ and Na+ was investigated with respect to a possible involvement of a previously unidentified specific transport pathway, because earlier studies showed that it cannot be explained on the basis of simple electrodiffusion. In particular,...

journal_title：Biophysical journal

authors： Richter S,Hamann J,Kummerow D,Bernhardt I

更新日期：1997-08-01 00:00:00

abstract：:In the budding yeast nucleus, transfer RNA (tRNA) genes are considered to localize in the vicinity of the nucleolus; however, the use of Hi-C and fluorescent repressor-operator system techniques has clearly indicated that the tRNA genes are distributed not only around the nucleolus but also at other nuclear locations....

journal_title：Biophysical journal

authors： Tokuda N

更新日期：2020-05-05 00:00:00

abstract：:Excised inside-out membrane patches are useful for studying the cGMP-activated ion channels that generate the electrical response to light in retinal rod cells. We show that strong ionic current across a patch changes the driving force on the current by altering the ionic concentration near the surface membrane, an ef...

journal_title：Biophysical journal

authors： Zimmerman AL,Karpen JW,Baylor DA

更新日期：1988-08-01 00:00:00

abstract：:Allosteric regulation of protein function is recognized to be widespread throughout biology; however, knowledge of allosteric mechanisms, the molecular changes within a protein that couple one binding site to another, is limited. Although mutagenesis is often used to probe allosteric mechanisms, we consider herein wha...

journal_title：Biophysical journal

authors： Carlson GM,Fenton AW

更新日期：2016-05-10 00:00:00

abstract：:We have studied the atomic level structure of mineralized articular cartilage with heteronuclear solid-state NMR, our aims being to identify the inorganic species present at the surfaces of the mineral crystals which may interact with the surrounding organic matrix and to determine which components of the organic matr...

journal_title：Biophysical journal

authors： Duer MJ,Friscić T,Murray RC,Reid DG,Wise ER

更新日期：2009-04-22 00:00:00

abstract：:We have monitored the organization and dynamics of the hemolytic peptide melittin in membranes containing cholesterol by utilizing the intrinsic fluorescence properties of its functionally important sole tryptophan residue and circular dichroism spectroscopy. The significance of this study is based on the fact that th...

journal_title：Biophysical journal

authors： Raghuraman H,Chattopadhyay A

更新日期：2004-10-01 00:00:00

abstract：:Cholesterol is an essential molecule in the membranes of mammalian cells. It is known to be distributed heterogeneously within the cells, between the bilayer leaflets, as well as between lateral domains within the bilayer. However, we do not know exactly how cholesterol is distributed and what forces drive this sortin...

journal_title：Biophysical journal

authors： Nyholm TKM,Jaikishan S,Engberg O,Hautala V,Slotte JP

更新日期：2019-01-22 00:00:00

abstract：:Although the structure, function, conformational dynamics, and controlled thermodynamics of proteins are manifested by their corresponding amino acid sequences, the natural rules for molecular design and their corresponding interplay remain obscure. In this study, we focused on the role of internal cavities of protein...

journal_title：Biophysical journal

authors： Maeno A,Sindhikara D,Hirata F,Otten R,Dahlquist FW,Yokoyama S,Akasaka K,Mulder FA,Kitahara R

更新日期：2015-01-06 00:00:00

abstract：:The absolute direction of the retinal chromophore of bacterio-rhodopsin relative to the membrane plane is investigated by using an optical second-harmonic interference technique. Compared with the known adsorbed geometry of free retinylidene Schiff base on a glass substrate, our data indicate the beta-ionone ring of t...

journal_title：Biophysical journal

authors： Huang JY,Lewis A

更新日期：1989-05-01 00:00:00

abstract：:Thermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations, however, is highly...

journal_title：Biophysical journal

authors： Seeliger D,de Groot BL

更新日期：2010-05-19 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:Twenty-seven mutant bacteriorhodopsin's were screened to determine the PKa for reversal of the permanent electric dipole moment. The photoelectric response of an aqueous purple-membrane suspension was used to determine the direction of the purple-membrane dipole moment as a function of pH. The pK(a) for the dipole rev...

journal_title：Biophysical journal

authors： Hsu KC,Rayfield GW,Needleman R

更新日期：1996-05-01 00:00:00

abstract：:Formamide-induced detubulation of rat ventricular myocytes was used to investigate the functional distribution of the Na/Ca exchanger (NCX) and Na/K-ATPase between the t-tubules and external sarcolemma. Detubulation resulted in a 32% decrease in cell capacitance, whereas cell volume was unchanged. Thus, the surface-to...

journal_title：Biophysical journal

authors： Despa S,Brette F,Orchard CH,Bers DM

更新日期：2003-11-01 00:00:00

abstract：:A delayed voltage-dependent K+ current endogenous to Xenopus oocytes has been investigated by the voltage-clamp technique. Both activation and inactivation of the K+ current are voltage-dependent processes. The K+ currents were activated when membrane potential was depolarized from a holding potential of -90 to -50 mV...

journal_title：Biophysical journal

authors： Lu L,Montrose-Rafizadeh C,Hwang TC,Guggino WB

更新日期：1990-06-01 00:00:00

abstract：:The distal side of the heme pocket, known to regulate ligand affinity, is shown to be directly involved in subunit interactions. Valency hybrids with oxygen or carbon monoxide bound to the reduced chain are used to model R-state hemoglobin with different distal perturbations. Electron paramagnetic resonance of the oxi...

journal_title：Biophysical journal

authors： Levy A,Sharma VS,Zhang L,Rifkind JM

更新日期：1992-03-01 00:00:00

abstract：:Huntington's disease is a heritable neurodegenerative disease that is caused by a CAG expansion in the first exon of the huntingtin gene. This expansion results in an elongated polyglutamine domain that increases the propensity of huntingtin exon-1 to form cross-β fibrils. Although the polyglutamine domain is importan...

journal_title：Biophysical journal

authors： Falk AS,Bravo-Arredondo JM,Varkey J,Pacheco S,Langen R,Siemer AB

更新日期：2020-11-17 00:00:00

abstract：:The wide use of lattice-sum strategies in biomolecular simulations has raised many questions on potential artifacts in these strategies. One interesting question is the artifacts in the counterion distributions of highly charged systems. As one would anticipate, Coulombic interactions under the periodic boundary condi...

journal_title：Biophysical journal

authors： Ye X,Cai Q,Yang W,Luo R

更新日期：2009-07-22 00:00:00