Abstract:
:We performed pressure-tuning hole-burning experiments on a modified cytochrome c protein in a glycerol/buffer glass. The shift and the broadening of the holes were investigated for various frequencies within the inhomogeneous band. On the basis of a simple model, we were able to estimate the interaction range between chromophore and protein. It is approximately 4.5 A. The parameters that enter the model are the compressibility, the static mean-square displacement, the inhomogeneous width, and the average spectral shift per pressure. From this result and from our experiments on pressure-induced denaturing, we conclude that water molecules have to be brought very close to the chromophore during the denaturation process.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Lesch H,Schlichter J,Friedrich J,Vanderkooi JMdoi
10.1016/S0006-3495(04)74124-7subject
Has Abstractpub_date
2004-01-01 00:00:00pages
467-72issue
1 Pt 1eissn
0006-3495issn
1542-0086pii
S0006-3495(04)74124-7journal_volume
86pub_type
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