Abstract:
:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to their antigens when probed with an AFM in the force mode. We could measure the rupture force between individual antibody-antigen complexes. The potential and limitations of this new approach for the measurement of individual antigen/antibody interactions and some possible applications are discussed.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJdoi
10.1016/S0006-3495(96)79814-4subject
Has Abstractpub_date
1996-05-01 00:00:00pages
2437-41issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(96)79814-4journal_volume
70pub_type
杂志文章abstract::In this work we have designed a simple system to investigate empirically the image contrast of tapping-mode atomic force microscopy (TMAFM). We modified the cantilever tips with protein molecules (bovine serum albumin or goat anti-biotin antibody) and used these protein-modified cantilevers to scan poly-L-lysine films...
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