A modular view of the diversity of cell-density-encoding schemes in bacterial quorum-sensing systems.

abstract：:We studied the photocurrents of a cyanobacterial rhodopsin Gloeobacter violaceus (GR) in Xenopus laevis oocytes and HEK-293 cells. This protein is a light-driven proton pump with striking similarities to marine proteorhodopsins, including the D121-H87 cluster of the retinal Schiff base counterion and a glutamate at po...

journal_title：Biophysical journal

authors： Vogt A,Wietek J,Hegemann P

更新日期：2013-11-05 00:00:00

abstract：:Molecular dynamics simulations of the tripeptide Ala-Phe-Ala-O-tert-butyl interacting with dimyristoylphosphatidylcholine lipid bilayers have been carried out. The lipid and aqueous environments of the peptide, the alkyl chain order, and the lipid and peptide dynamics have been investigated with use of density profile...

journal_title：Biophysical journal

authors： Damodaran KV,Merz KM Jr,Gaber BP

更新日期：1995-10-01 00:00:00

abstract：:Elongation of the mitotic spindle during anaphase B contributes to chromosome segregation in many cells. Here, we quantitatively test the ability of two models for spindle length control to describe the dynamics of anaphase B spindle elongation using experimental data from Drosophila embryos. In the slide-and-flux-or-...

journal_title：Biophysical journal

authors： Brust-Mascher I,Civelekoglu-Scholey G,Scholey JM

更新日期：2015-04-21 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Thin filament regulation of contraction is thought to involve the binding of two activating ligands: Ca2+ and strongly bound cross-bridges. The specific cross-bridge states required to promote thin filament activation have not been identified. This study examines the relationship between cross-bridge cycling and thin ...

journal_title：Biophysical journal

authors： Wahr PA,Metzger JM

更新日期：1999-04-01 00:00:00

abstract：:Aggregated Markov models are a widely used tool to model patch clamp data measured from single ion channels. These channels must obey the principle of detailed balance in thermodynamic equilibrium; otherwise, the channel is driven by an external source of energy. We investigate the power of a likelihood ratio test for...

journal_title：Biophysical journal

authors： Wagner M,Timmer J

更新日期：2000-12-01 00:00:00

abstract：:The freshwater polyp Hydra can regenerate from tissue fragments or random cell aggregates. We show that the axis-defining step ("symmetry breaking") of regeneration requires mechanical inflation-collapse oscillations of the initial cell ball. We present experimental evidence that axis definition is retarded if these o...

journal_title：Biophysical journal

authors： Soriano J,Rüdiger S,Pullarkat P,Ott A

更新日期：2009-02-18 00:00:00

abstract：:Large and rapid changes in light scattering accompany secretion from nerve terminals of the mammalian neurohypophysis (posterior pituitary). In the mouse, these intrinsic optical signals are intimately related to the arrival of the action potential E-wave and the release of arginine vasopressin and oxytocin (S-wave). ...

journal_title：Biophysical journal

authors： Kim GH,Kosterin P,Obaid AL,Salzberg BM

更新日期：2007-05-01 00:00:00

abstract：:We have used admittance analysis together with the black lipid membrane technique to analyze electrogenic reactions within the Na(+) branch of the reaction cycle of the Na(+)/K(+)-ATPase. ATP release by flash photolysis of caged ATP induced changes in the admittance of the compound membrane system that are associated ...

journal_title：Biophysical journal

authors： Babes A,Fendler K

更新日期：2000-11-01 00:00:00

abstract：:The mechanisms that limit the speed at which striated muscles relax are poorly understood. This work presents, to our knowledge, novel simulations that show that the time course of relaxation is accelerated by interfilamentary movement resulting from series compliance; force drops faster when myosin heads move relativ...

journal_title：Biophysical journal

authors： Campbell KS

更新日期：2016-02-02 00:00:00

abstract：:Giant bilayer vesicles were reconstituted from several lipids and lipid/cholesterol (CHOL) mixtures: stearolyloleoylphosphatidylcholine (SOPC), bovine sphingomyelin (BSM), diarachidonylphosphatidylcholine (DAPC), SOPC/CHOL, BSM/CHOL, DAPC/CHOL, and extracted red blood cell (RBC) lipids with native cholesterol. Single-...

journal_title：Biophysical journal

authors： Needham D,Nunn RS

更新日期：1990-10-01 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:A study on clonal growth in Chinese hamster ovary (CHO) cells was conducted after exposure to optical trapping wavelengths using Nd:YAG (1064 nm) and tunable titanium-sapphire (700-990 nm) laser microbeam optical traps. The nuclei of cells were exposed to optical trapping forces at various wavelengths, power densities...

journal_title：Biophysical journal

authors： Liang H,Vu KT,Krishnan P,Trang TC,Shin D,Kimel S,Berns MW

更新日期：1996-03-01 00:00:00

abstract：:The mitochondrial channel, VDAC, forms large (3 nm in diameter) aqueous pores through membranes. We measured ATP flow (using the luciferin/luciferase method) through these channels after reconstitution into planar phospholipid membranes. In the open state of VDAC, as many as 2 x 10(6) ATP molecules can flow through on...

journal_title：Biophysical journal

authors： Rostovtseva T,Colombini M

更新日期：1997-05-01 00:00:00

abstract：:Despite growing interest in light-driven ion pumps for use in optogenetics, current estimates of their transport rates span two orders of magnitude due to challenges in measuring slow transport processes and determining protein concentration and/or orientation in membranes in vitro. In this study, we report, to our kn...

journal_title：Biophysical journal

authors： Feroz H,Ferlez B,Lefoulon C,Ren T,Baker CS,Gajewski JP,Lugar DJ,Gaudana SB,Butler PJ,Hühn J,Lamping M,Parak WJ,Hibberd JM,Kerfeld CA,Smirnoff N,Blatt MR,Golbeck JH,Kumar M

更新日期：2018-07-17 00:00:00

abstract：:Cis-parinaric acid (PA) binds to a hydrophobic pocket formed between the heavy chain of myosin subfragment-1 (S1) and the 41-residue N-terminal of essential light chain 1 (A1). The binding is strong (Ka = 5.6 x 10(7) M-1) and rigid (polarization = 0.334). PA does not bind to myofibrils in which A1 has been extracted o...

journal_title：Biophysical journal

authors： Xiao M,Borejdo J

更新日期：1997-05-01 00:00:00

abstract：:The process by which an organism changes the composition of its membranal fatty acids in response to growth temperature, so as to maintain optimal membrane functioning, is known as homeoviscous adaptation (HA). One expression of HA is the constancy of the fluorescence polarization (P) of the lipophilic probe 1,6-diphe...

journal_title：Biophysical journal

authors： Parola AH,Ibdah M,Gill D,Zaritsky A

更新日期：1990-03-01 00:00:00

abstract：:Extraction from simulations of the area compressibility moduli of the monolayers in a bilayer is considered theoretically. A statistical mechanical derivation shows that the bilayer modulus is the sum of the two monolayer moduli, as is often supposed but contrary to a recent study. Seemingly plausible assumptions rega...

journal_title：Biophysical journal

authors： Nagle JF

更新日期：2019-09-17 00:00:00

abstract：:A number of models proposed to account for the sodium conductance changes are shown to fall into two classes. The Hodgkin-Huxley (HH) model falls into a class (I) in which the conductance depends on two or more independent variables controlled by independent processes. The Mullins, Hoyt, and Goldman models fall into c...

journal_title：Biophysical journal

authors： Hoyt RC

更新日期：1968-10-01 00:00:00

abstract：:Cholesterol, due to its condensing effect, is considered an important regulator of membrane thickness. Other sterols, due to their structural similarities to cholesterol, are often assumed to have a universal effect on membrane properties similar to the condensing effect of cholesterol, albeit possibly to different de...

journal_title：Biophysical journal

authors： Hung WC,Lee MT,Chung H,Sun YT,Chen H,Charron NE,Huang HW

更新日期：2016-05-10 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:We argue that membrane viscosity, η(m), plays a prominent role in the thermal fluctuation dynamics of micron-scale lipid domains. A theoretical expression is presented for the timescales of domain shape relaxation, which reduces to the well-known η(m) = 0 result of Stone and McConnell in the limit of large domain size...

journal_title：Biophysical journal

authors： Camley BA,Esposito C,Baumgart T,Brown FL

更新日期：2010-09-22 00:00:00

abstract：:We have studied the low-resolution solution conformation of a Holliday (or four-way) DNA junction by using small-angle x-ray scattering, sedimentation velocity, and computational modeling techniques. The scattering data were analyzed in two independent ways: firstly, by rigid-body modeling of the scattering data using...

journal_title：Biophysical journal

authors： Nöllmann M,Stark WM,Byron O

更新日期：2004-05-01 00:00:00

abstract：:A triple mutant of sperm whale myoglobin (Mb) [Leu(B10) --> Tyr, His(E7) --> Gln, and Thr(E10) --> Arg, called Mb-YQR], investigated by stopped-flow, laser photolysis, crystallography, and molecular dynamics (MD) simulations, proved to be quite unusual. Rebinding of photodissociated NO, O2, and CO from within the prot...

journal_title：Biophysical journal

authors： Brunori M,Cutruzzolà F,Savino C,Travaglini-Allocatelli C,Vallone B,Gibson QH

更新日期：1999-03-01 00:00:00

abstract：:The sarcoplasmic reticulum channel (ryanodine receptor) from cardiac myocytes was reconstituted into planar lipid bilayers consisting of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) in varying ratios. The channel activity parameters, i.e., open probability an...

journal_title：Biophysical journal

authors： Cannon B,Hermansson M,Györke S,Somerharju P,Virtanen JA,Cheng KH

更新日期：2003-08-01 00:00:00

abstract：:A newly developed microscope using acousto-optic tunable filters (AOTFs) was used to generate in vivo hemoglobin saturation (SO2) and oxygen tension (PO2) maps in the cerebral cortex of mice. SO2 maps were generated from the spectral analysis of reflected absorbance images collected at different wavelengths, and PO2 m...

journal_title：Biophysical journal

authors： Shonat RD,Wachman ES,Niu W,Koretsky AP,Farkas DL

更新日期：1997-09-01 00:00:00

abstract：:Diphtheria toxin (DT) contains separate domains for receptor-specific binding, translocation, and enzymatic activity. After binding to cells, DT is taken up into endosome-like acidic compartments where the translocation domain inserts into the endosomal membrane and releases the catalytic domain into the cytosol. The ...

journal_title：Biophysical journal

authors： Kent MS,Yim H,Murton JK,Satija S,Majewski J,Kuzmenko I

更新日期：2008-03-15 00:00:00

abstract：:The quantitation of human granulocyte movement using a stochastic differential equation is described. The method has the potential to distinguish both positive and negative chemotaxis. Analysis and information concerning cell movements can be obtained for any point in time and distance for the duration of the experime...

journal_title：Biophysical journal

authors： Boyarsky A,Noble PB,Peterson SC

更新日期：1976-03-01 00:00:00

abstract：:Many traditional approaches to the analysis of sedimentation velocity data work poorly with data for low-molecular-weight solutes, which have sedimentation boundaries that are severely broadened by diffusion. An approach that has previously had some success is to directly fit these broad boundaries to approximate solu...

journal_title：Biophysical journal

authors： Philo JS

更新日期：1997-01-01 00:00:00

abstract：:Synchrotron x-ray scattering measurements were performed on dilute solutions of the purified hemocyanin subunit (Bsin1) from scorpion (Buthus sindicus) and the N-terminal functional unit (Rta) from a marine snail (Rapana thomasiana). The model-independent approach based on spherical harmonics was applied to calculate ...

journal_title：Biophysical journal

authors： Grossmann JG,Ali SA,Abbasi A,Zaidi ZH,Stoeva S,Voelter W,Hasnain SS

更新日期：2000-02-01 00:00:00