Regulation of calcium channel activity by lipid domain formation in planar lipid bilayers.

abstract：:We used time-resolved Förster resonance energy transfer, circular dichroism, and molecular dynamics simulation to investigate the structural dependence of synaptotagmin 1's intrinsically disordered region (IDR) on phosphorylation and dielectric constant. We found that a peptide corresponding to the full-length IDR seq...

journal_title：Biophysical journal

authors： Fealey ME,Binder BP,Uversky VN,Hinderliter A,Thomas DD

更新日期：2018-02-06 00:00:00

abstract：:EXACT FORMULAS ARE DERIVED FOR THE ENERGY CHANGE OF A DIPOLE SYSTEM WITH TWO ENERGY STATES (OR BANDS) IN A CHANGING FIELD IN TWO CASES: (a) no dipole flip-flop and (b) dipole flip-flop caused by stimulation. Based on these formulas, the positive and negative heats are calculated. The results are in good agreement with...

journal_title：Biophysical journal

authors： Wei LY

更新日期：1972-09-01 00:00:00

abstract：:Fast Ca(2+) release kinetics were measured in cardiac sarcoplasmic reticulum vesicles actively loaded with Ca(2+). Release was induced in solutions containing 1.2 mM free ATP and variable free [Ca(2+)] and [Mg(2+)]. Release rate constants (k) were 10-fold higher at pCa 6 than at pCa 5 whereas Ryanodine binding was hig...

journal_title：Biophysical journal

authors： Sánchez G,Hidalgo C,Donoso P

更新日期：2003-04-01 00:00:00

abstract：:A continuum model is provided of the free energy terms that contribute to the molecular association of ricin A-chain (RTA) with the rRNA substrate and several small ligands. The model for RTA interactions with the RNA was taken from a previously proposed complex containing a 29-mer oligonucleotide hairpin (. Proteins ...

journal_title：Biophysical journal

authors： Olson MA,Cuff L

更新日期：1999-01-01 00:00:00

abstract：:The influence of the amino acid residues surrounding the flavin ring in the flavodoxin of the cyanobacterium Anabaena PCC 7119 on the electron spin density distribution of the flavin semiquinone was examined in mutants of the key residues Trp(57) and Tyr(94) at the FMN binding site. Neutral semiquinone radicals of the...

journal_title：Biophysical journal

authors： Medina M,Lostao A,Sancho J,Gómez-Moreno C,Cammack R,Alonso PJ,Martínez JI

更新日期：1999-09-01 00:00:00

abstract：:Purified cytochrome c oxidase CO complex from beef heart has been studied by Fourier transform infrared absorbance difference spectroscopy. Photolysis at 10-20 Kelvin results in dissociation of a3FeCO, formation of CuBCO, and perturbation of the a3-heme and CuB complex. The vibrational perturbation spectrum between 90...

journal_title：Biophysical journal

authors： Park S,Pan LP,Chan SI,Alben JO

更新日期：1996-08-01 00:00:00

abstract：:We present a probability density approach to modeling localized Ca2+ influx via L-type Ca2+ channels and Ca2+-induced Ca2+ release mediated by clusters of ryanodine receptors during excitation-contraction coupling in cardiac myocytes. Coupled advection-reaction equations are derived relating the time-dependent probabi...

journal_title：Biophysical journal

authors： Williams GS,Huertas MA,Sobie EA,Jafri MS,Smith GD

更新日期：2007-04-01 00:00:00

abstract：:As sketched in Fig. 1, a current molecular dynamics computer simulation of a lipid bilayer fails to capture significant features of the macroscopic system, including long wavelength undulations. Such fluctuations are intrinsically connected to the value of the macroscopic (or thermodynamic) surface tension (cf. Eqs. 1...

journal_title：Biophysical journal

authors： Feller SE,Pastor RW

更新日期：1996-09-01 00:00:00

abstract：:We present here the parmbsc0 force field, a refinement of the AMBER parm99 force field, where emphasis has been made on the correct representation of the alpha/gamma concerted rotation in nucleic acids (NAs). The modified force field corrects overpopulations of the alpha/gamma = (g+,t) backbone that were seen in long ...

journal_title：Biophysical journal

authors： Pérez A,Marchán I,Svozil D,Sponer J,Cheatham TE 3rd,Laughton CA,Orozco M

更新日期：2007-06-01 00:00:00

abstract：:We report here a multiprotein blind test of a computer method to predict native protein structures based solely on an all-atom physics-based force field. We use the AMBER 96 potential function with an implicit (GB/SA) model of solvation, combined with replica-exchange molecular-dynamics simulations. Coarse conformatio...

journal_title：Biophysical journal

authors： Shell MS,Ozkan SB,Voelz V,Wu GA,Dill KA

更新日期：2009-02-01 00:00:00

abstract：:We describe a model that relates the maximum shortening velocity of a muscle fiber, Vm, to the kinetics of the dissociation of a myosin head from actin. At Vm, the positive work exerted by cross-bridges attached in the powerstroke must be balanced by cross-bridges that have been carried by movement of the filaments in...

journal_title：Biophysical journal

authors： Cooke R,White H,Pate E

更新日期：1994-03-01 00:00:00

abstract：:It has been repeatedly noted that the change of conformation of the molecules that serve as the ion-selective channels for sodium and potassium conductance in the nerve membrane will be accompanied by a change in the dipole moment of the molecule. This time-dependent change of dipole moment will produce transient curr...

journal_title：Biophysical journal

authors： Strandberg MW

更新日期：1977-09-01 00:00:00

abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...

journal_title：Biophysical journal

authors： Butler WL

更新日期：1972-07-01 00:00:00

abstract：:The orientational order as determined by 2H NMR and the infrared frequencies of the C--H stretching modes of the methylene groups have been measured for several systems (POPC, POPC/cholesterol and POPE), all in the fluid phase, and then were compared; this work reveals an unexpected linear correlation between them. Th...

journal_title：Biophysical journal

authors： Kodati VR,Lafleur M

更新日期：1993-01-01 00:00:00

abstract：:Long time series of monosynaptic Ia-afferent to alpha-motoneuron reflexes were recorded in the L7 or S1 ventral roots in the cat. Time series were collected before and after spinalization at T13 during constant amplitude stimulations of group Ia muscle afferents in the triceps surae muscle nerves. Using autocorrelatio...

journal_title：Biophysical journal

authors： Chang T,Schiff SJ,Sauer T,Gossard JP,Burke RE

更新日期：1994-08-01 00:00:00

abstract：:Combining an azobenzene chromophore with the bis-cysteinyl active-site sequence of the protein disulfide isomerase (PDI) we constructed a simple but promising model for allosteric conformational rearrangements. Paralleling cellular signaling events, an external trigger, here absorption of a photon, leads to a structur...

journal_title：Biophysical journal

authors： Löweneck M,Milbradt AG,Root C,Satzger H,Zinth W,Moroder L,Renner C

更新日期：2006-03-15 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Chemical synaptic transmission is a fundamental component of interneuronal communications in the central nervous system (CNS). Discharge of a presynaptic vesicle containing a few thousand molecules (a quantum) of neurotransmitter into the synaptic cleft generates a transmitter concentration signal that drives postsyna...

journal_title：Biophysical journal

authors： Uteshev VV,Pennefather PS

更新日期：1997-03-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) are important membrane proteins that mediate cellular signaling and represent primary targets for about one-third of currently marketed drugs. Recent x-ray crystallographic studies identified distinct conformations of GPCRs in the active and inactive states. An allosteric sodium ion...

journal_title：Biophysical journal

authors： Miao Y,Caliman AD,McCammon JA

更新日期：2015-04-07 00:00:00

abstract：:Migratory cells are known to adapt to environments that contain wide-ranging levels of chemoattractant. Although biochemical models of adaptation have been previously proposed, here, we discuss a different mechanism based on mechanosensing, in which the interaction between biochemical signaling and cell tension facili...

journal_title：Biophysical journal

authors： Zmurchok C,Collette J,Rajagopal V,Holmes WR

更新日期：2020-10-20 00:00:00

abstract：:It has been shown in previous studies that cell poration (i.e., reversible permeabilization of cell membrane) and cell fusion can be induced by applying a pulse (or pulses) of high-intensity DC (direct current) electric field. Recently we suggested that such electro-poration or electro-fusion can also be accomplished ...

journal_title：Biophysical journal

authors： Chang DC

更新日期：1989-10-01 00:00:00

abstract：:This paper is concerned with the nature of the transmembrane potential induced in an inactive nerve fiber lying in an impressed potential field within a nerve trunk. The impressed potential field is assumed to be produced by the synchronous activity of other fibers within the trunk; an expression for the induced trans...

journal_title：Biophysical journal

authors： Clark JW Jr,Plonsey R

更新日期：1971-03-01 00:00:00

abstract：:Tet repressor (TetR) is involved in the most abundant mechanism of tetracycline (Tc) resistance of gram-negative bacteria. Raman spectra were measured for the class D TetR protein, for an oligodeoxyribonucleotide with sequence corresponding to operator site O1, and for the TetR:oligonucleotide complex. TetR forms a co...

journal_title：Biophysical journal

authors： Krafft C,Hinrichs W,Orth P,Saenger W,Welfle H

更新日期：1998-01-01 00:00:00

abstract：:Recent studies suggest that phosphoinositide kinases may participate in intracellular trafficking or exocytotic events. Because both of these events ultimately require fusion of biological membranes, the susceptibility of membranes containing polyphosphoinositides (PPIs) to divalent cation-induced fusion was investiga...

journal_title：Biophysical journal

authors： Summers SA,Guebert BA,Shanahan MF

更新日期：1996-12-01 00:00:00

abstract：:Various differential polarization images or Mueller images of model objects are generated using the equations derived in the previous paper (paper I of this series). These calculated images include models of the higher-order organization of metaphase chromosomes, and show the applicability of the differential polariza...

journal_title：Biophysical journal

authors： Kim M,Ulibarri L,Bustamante C

更新日期：1987-12-01 00:00:00

abstract：:We have used admittance analysis together with the black lipid membrane technique to analyze electrogenic reactions within the Na(+) branch of the reaction cycle of the Na(+)/K(+)-ATPase. ATP release by flash photolysis of caged ATP induced changes in the admittance of the compound membrane system that are associated ...

journal_title：Biophysical journal

authors： Babes A,Fendler K

更新日期：2000-11-01 00:00:00

abstract：:The one-dimensional information of genomic DNA is hierarchically packed inside the eukaryotic cell nucleus and organized in a three-dimensional (3D) space. Genome-wide chromosome conformation capture (Hi-C) methods have uncovered the 3D genome organization and revealed multiscale chromatin domains of compartments and ...

journal_title：Biophysical journal

authors： Shinkai S,Sugawara T,Miura H,Hiratani I,Onami S

更新日期：2020-05-05 00:00:00

abstract：:PROPPINs (β-propellers that bind polyphosphoinositides) are a family of PtdIns3P- and PtdIns(3,5)P2-binding proteins that play an important role in autophagy. We analyzed PROPPIN-membrane binding through isothermal titration calorimetry (ITC), stopped-flow measurements, mutagenesis studies, and molecular dynamics (MD)...

journal_title：Biophysical journal

authors： Busse RA,Scacioc A,Krick R,Pérez-Lara Á,Thumm M,Kühnel K

更新日期：2015-05-05 00:00:00

abstract：:Using homonuclear (1)H NOESY spectra, with chemical shifts, (3)JH(N)H(α) scalar couplings, residual dipolar couplings, and (1)H-(15)N NOEs, we have optimized and validated the conformational ensembles of the amyloid-β 1-40 (Aβ40) and amyloid-β 1-42 (Aβ42) peptides generated by molecular dynamics simulations. We find t...

journal_title：Biophysical journal

authors： Ball KA,Phillips AH,Wemmer DE,Head-Gordon T

更新日期：2013-06-18 00:00:00

abstract：:Model compound studies in the literature show how Hofmeister ion interactions affect protein stability. Although model compound results are typically obtained as salting-out constants, they can be used to find out how the interactions affect protein stability. The null point in the Hofmeister series, which divides pro...

journal_title：Biophysical journal

authors： Baldwin RL

更新日期：1996-10-01 00:00:00