Membrane Tension Can Enhance Adaptation to Maintain Polarity of Migrating Cells.

abstract：:We report molecular dynamics simulations in the explicit membrane environment of a small membrane-embedded protein, sarcolipin, which regulates the sarcoplasmic reticulum Ca-ATPase activity in both cardiac and skeletal muscle. In its monomeric form, we found that sarcolipin adopts a helical conformation, with a comput...

journal_title：Biophysical journal

authors： Shi L,Cembran A,Gao J,Veglia G

更新日期：2009-05-06 00:00:00

abstract：:The TRPV1 cation nonselective ion channel plays an essential role in thermosensation and perception of other noxious stimuli. TRPV1 can be activated by low extracellular pH, high temperature, or naturally occurring pungent molecules such as allicin, capsaicin, or resiniferatoxin. Its noxious thermal sensitivity makes ...

journal_title：Biophysical journal

authors： Ladrón-de-Guevara E,Dominguez L,Rangel-Yescas GE,Fernández-Velasco DA,Torres-Larios A,Rosenbaum T,Islas LD

更新日期：2020-02-25 00:00:00

abstract：:Based on differences between the x-ray crystal structures of ligand-bound and unbound forms, the activation of the erythropoietin receptor (EPOR) was initially proposed to involve a cross-action scissorlike motion. However, the validity of the motions involved in the scissorlike model has been recently challenged. Her...

journal_title：Biophysical journal

authors： Corbett MSP,Mark AE,Poger D

更新日期：2017-02-28 00:00:00

abstract：:Molecular sensors based on intramolecular Förster resonance energy transfer (FRET) have become versatile tools to monitor regulatory molecules in living tissue. However, their use is often compromised by low signal strength and excessive noise. We analyzed signal/noise (SNR) aspects of spectral FRET analysis methods, ...

journal_title：Biophysical journal

authors： Woehler A,Wlodarczyk J,Neher E

更新日期：2010-10-06 00:00:00

abstract：:Staphylococcus aureus expresses numerous virulence factors that aid in immune evasion. The four-domain staphylococcal immunoglobulin binding (Sbi) protein interacts with complement component 3 (C3) and its thioester domain (C3d)-containing fragments. Recent structural data suggested two possible modes of binding of Sb...

journal_title：Biophysical journal

authors： Gorham RD Jr,Rodriguez W,Morikis D

更新日期：2014-03-04 00:00:00

abstract：:A fundamental question concerning the ClC Cl-/H+ antiporters is the nature of their proton transport (PT) pathway. We addressed this issue by using a novel computational methodology capable of describing the explicit PT dynamics in the ClC-ec1 protein. The main result is that the Glu203 residue delivers a proton from ...

journal_title：Biophysical journal

authors： Wang D,Voth GA

更新日期：2009-07-08 00:00:00

abstract：:A Brownian dynamics (BD) model described in the accompanying paper (Klenin, K., H. Merlitz, and J. Langowski. 1998. A Brownian dynamics program for the simulation of linear and circular DNA, and other wormlike chain polyelectrolytes. Biophys. J. 74:000-000) has been used for computing the end-to-end distance distribut...

journal_title：Biophysical journal

authors： Merlitz H,Rippe K,Klenin KV,Langowski J

更新日期：1998-02-01 00:00:00

abstract：:The formation and function of the mitotic spindle depends upon force generation by multiple molecular motors and by the dynamics of microtubules, but how these force-generating mechanisms relate to one another is unclear. To address this issue we have modeled the separation of spindle poles as a function of time durin...

journal_title：Biophysical journal

authors： Cytrynbaum EN,Scholey JM,Mogilner A

更新日期：2003-02-01 00:00:00

abstract：:RNA functions are often kinetically controlled. The folding kinetics of RNAs involves global structural changes and local nucleotide movement, such as base flipping. The most elementary step in RNA folding is the closing and opening of a basepair. By integrating molecular dynamics simulation, master equation, and kine...

journal_title：Biophysical journal

authors： Wang F,Sun LZ,Cai P,Chen SJ,Xu X

更新日期：2019-11-05 00:00:00

abstract：:We have examined the morphology and osmotic properties of large unilamellar vesicles (LUVs) prepared by extrusion. Contrary to expectations, we observe by cryo-electron microscopy that such vesicles, under isoosmotic conditions, are non-spherical. This morphology appears to be a consequence of vesicle passage through ...

journal_title：Biophysical journal

authors： Mui BL,Cullis PR,Evans EA,Madden TD

更新日期：1993-02-01 00:00:00

abstract：:The cardiac muscle protein titin, responsible for developing passive elasticity and extensibility of muscle, possesses about 40 immunoglobulin-like (Ig) domains in its I-band region. Atomic force microscopy (AFM) and steered molecular dynamics (SMD) have been successfully combined to investigate the reversible unfoldi...

journal_title：Biophysical journal

authors： Gao M,Wilmanns M,Schulten K

更新日期：2002-12-01 00:00:00

abstract：:We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring pe...

journal_title：Biophysical journal

authors： Kelly CV,Baird BA,Craighead HG

更新日期：2011-04-06 00:00:00

abstract：:Integrin-mediated signaling is crucial for cell-substrate adhesion and can be triggered from both intra- and extracellular interactions. Although talin binding is sufficient for inside-out activation of integrin, other cytoplasmic proteins such as α-actinin and filamin can directly interfere with talin-mediated integr...

journal_title：Biophysical journal

authors： Shams H,Mofrad MRK

更新日期：2017-08-22 00:00:00

abstract：:The aim of this study was to gain insight into how interactions between proteins and lipids in membranes are sensed at the protein-lipid interface. As a probe to analyze this interface, we used deuterium-labeled acyl chains that were covalently linked to a model transmembrane peptide. First, a perdeuterated palmitoyl ...

journal_title：Biophysical journal

authors： Nyholm TK,van Duyl B,Rijkers DT,Liskamp RM,Killian JA

更新日期：2011-10-19 00:00:00

abstract：:Diverse microbial communities coordinate group behaviors through signal exchange, such as the exchange of acyl-homoserine lactones (AHLs) by Gram-negative bacteria. Cellular communication is prone to interference by neighboring microbes. One mechanism of interference is signal destruction through the production of an ...

journal_title：Biophysical journal

authors： Silva KP,Chellamuthu P,Boedicker JQ

更新日期：2017-03-14 00:00:00

abstract：:The relation between the dynamic properties of the haptenic site of lipid haptens and the phase transition of the host lattice was investigated using head group spin-labeled phosphatidylethanolamines, that is, spin-label lipid haptens (Brûlet, P., and H. M. McConnell, 1976, Proc. Natl. Acad. Sci. USA., 73:2977-2981; B...

journal_title：Biophysical journal

authors： Takeshita K,Utsumi H,Hamada A

更新日期：1987-08-01 00:00:00

abstract：:Comparison of the electron spin resonance spectra of phosphatidylcholines spin-labeled in the sn-2 chain at a position close to the polar region and close to the methyl terminus indicate that symmetrical saturated diacyl phosphatidylcholines with odd and even chain lengths from 13 to 20 C-atoms (and probably also 12 C...

journal_title：Biophysical journal

authors： Swamy MJ,Marsh D

更新日期：1995-10-01 00:00:00

abstract：:Using glycerinated spasmoneme of giant Zoothamnium sp., the physical properties of spasmoneme before and after Ca2+-induced contraction (pCa 4.5) were investigated. The volume change of spasmoneme contraction was measured under zero tension. The length and diameter decreased by about 50% of their initial value as a re...

journal_title：Biophysical journal

authors： Moriyama Y,Okamoto H,Asai H

更新日期：1999-02-01 00:00:00

abstract：:The interaction between protein molecules and the hydroxyapatite (HAP) crystal is an important research topic in many fields. However, the nature of their noncovalent bonding is still not clear at the atomic level. In this work, molecular dynamics simulation, steered molecular dynamics simulation, and quantum chemistr...

journal_title：Biophysical journal

authors： Dong X,Wang Q,Wu T,Pan H

更新日期：2007-08-01 00:00:00

abstract：:The cellular response to external mechanical forces has important effects on numerous biological phenomena. The sequences of molecular events that underlie the observed changes in cellular properties have yet to be elucidated in detail. Here we have detected the responses of a cultured cell against locally applied cyc...

journal_title：Biophysical journal

authors： Watanabe-Nakayama T,Machida SI,Harada I,Sekiguchi H,Afrin R,Ikai A

更新日期：2011-02-02 00:00:00

abstract：:L-type (alpha(1C)) calcium channels inactivate rapidly in response to localized elevation of intracellular Ca(2+), providing negative Ca(2+) feedback in a diverse array of biological contexts. The dominant Ca(2+) sensor for such Ca(2+)-dependent inactivation has recently been identified as calmodulin, which appears to...

journal_title：Biophysical journal

authors： Peterson BZ,Lee JS,Mulle JG,Wang Y,de Leon M,Yue DT

更新日期：2000-04-01 00:00:00

abstract：:The genetic map of T4 (and T2) bacteriophage is circular but the DNA molecule that is liberated by phenol extraction is a linear duplex of polynucleotide chains. If the genetic map is related to the physical structure of the DNA molecule, the problem arises as to how a linear molecule can give rise to a circular map. ...

journal_title：Biophysical journal

authors： THOMAS CA Jr,RUBENSTEIN I

更新日期：1964-03-01 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These chang...

journal_title：Biophysical journal

authors： Miake-Lye RC,Doniach S,Hodgson KO

更新日期：1983-03-01 00:00:00

abstract：:Microscopic calcium (Ca2+) events (such as Ca2+ sparks) are an important area for study, as they help clarify the mechanism(s) underlying intracellular signaling. In the heart, Ca2+ sparks occur as a result of Ca2+ release from the sarcoendoplasmic reticulum, via ryanodine receptor channels. Measurement of Ca2+ spark ...

journal_title：Biophysical journal

authors： Kong CH,Soeller C,Cannell MB

更新日期：2008-12-15 00:00:00

abstract：:The primary drivers of yeast endocytosis are actin polymerization and curvature-generating proteins, such as clathrin and BAR domain proteins. Previous work has indicated that these factors may not be capable of generating the forces necessary to overcome turgor pressure. Thus local reduction of the turgor pressure, v...

journal_title：Biophysical journal

authors： Scher-Zagier JK,Carlsson AE

更新日期：2016-12-20 00:00:00

abstract：:The traditional theory of transport across capillary membranes via a laminar Poiseuille flow is shown to be invalid. It is demonstrated that the random, diffusive nature of the molecular flow and interactions with the "pore" walls play an important role in the transport process. Neither the continuum Navier-Stokes the...

journal_title：Biophysical journal

authors： Schindler AM,Iberall AS

更新日期：1973-08-01 00:00:00

abstract：:Measurements of voltage relaxations following brief charge-pulses applied to lipid bilayers have been performed at different hydrostatic pressures in the presence of the neutral carriers cyclo (D-Val-L-Pro-L-Val-D-Pro)3(PV) and valinomycin. From double-exponential relaxations observed in membranes containing PV-K+ com...

journal_title：Biophysical journal

authors： Benz R,Conti F

更新日期：1986-07-01 00:00:00

abstract：:Glasses used to fabricate patch pipettes may release components which affect ion channels (Cota, G., and C.M. Armstrong. 1988. Biophys. J. 53:107-109; Furman, R.E., and J.C. Tanaka. 1988. Biophys. J. 53:287-292; Rojas, L., and C. Zuazaga. 1988. Neurosci. Lett. 88:39-44). The gating properties of maxi K+ channels from ...

journal_title：Biophysical journal

authors： Copello J,Simon B,Segal Y,Wehner F,Ramanujam VM,Alcock N,Reuss L

更新日期：1991-10-01 00:00:00

abstract：:The three-dimensional structure of chromatin affects DNA accessibility and is therefore a key regulator of gene expression. However, the path of the DNA between consecutive nucleosomes, and the resulting chromatin fiber organization remain controversial. The conformational space available for the folding of the nucleo...

journal_title：Biophysical journal

authors： Stehr R,Schöpflin R,Ettig R,Kepper N,Rippe K,Wedemann G

更新日期：2010-03-17 00:00:00