Understanding adsorption-desorption dynamics of BMP-2 on hydroxyapatite (001) surface.

abstract：:In this work we have designed a simple system to investigate empirically the image contrast of tapping-mode atomic force microscopy (TMAFM). We modified the cantilever tips with protein molecules (bovine serum albumin or goat anti-biotin antibody) and used these protein-modified cantilevers to scan poly-L-lysine films...

journal_title：Biophysical journal

authors： You H,Yu L

更新日期：1997-12-01 00:00:00

abstract：:A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...

journal_title：Biophysical journal

authors： Paillotin G,Geacintov NE,Breton J

更新日期：1983-10-01 00:00:00

abstract：:Prior work has shown that concentration profiles of platelets in flowing whole blood and of platelet-sized beads in flowing blood suspensions can include near-wall excesses. A model to describe this phenomenon was built about a single-component convective diffusion equation. To incorporate redistribution to preferred ...

journal_title：Biophysical journal

authors： Yeh C,Calvez AC,Eckstein EC

更新日期：1994-09-01 00:00:00

abstract：:During protein synthesis dictated by the codon sequence of messenger RNA, the ribosome selects aminoacyl-tRNA (aa-tRNA) with high accuracy, the exact mechanism of which remains elusive. By using a single-molecule fluorescence resonance energy transfer method coupled with fluorescence emission anisotropy, we provide ev...

journal_title：Biophysical journal

authors： Mishra PP,Qureshi MT,Ren W,Lee TH

更新日期：2010-12-01 00:00:00

abstract：:The oligomeric structure was determined for four recombinant kinesin derivatives containing N-terminal fragments of the kinesin alpha-subunit. Some of the proteins were dimeric (two-headed) molecules with mechanochemical properties similar to those of intact kinesin. Comparison of the primary and quaternary structures...

journal_title：Biophysical journal

authors： Gelles J,Berliner E,Young EC,Mahtani HK,Perez-Ramirez B,Anderson K

更新日期：1995-04-01 00:00:00

abstract：:Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligo...

journal_title：Biophysical journal

authors： Pannwitt S,Stangl M,Schneider D

更新日期：2019-11-05 00:00:00

abstract：:A pulsed source neutron spectrometer has been used to measure vibrational spectra (20-4000 cm-1) of dry and hydrated type I collagen fibers, and of two model polypeptides, polyproline II and (prolyl-prolyl-glycine)10, at temperatures of 30 and 120 K. the collagen spectra provide the first high resolution neutron views...

journal_title：Biophysical journal

authors： Middendorf HD,Hayward RL,Parker SF,Bradshaw J,Miller A

更新日期：1995-08-01 00:00:00

abstract：:The prion protein (PrP), widely recognized to misfold into the causative agent of the transmissible spongiform encephalopathies, has previously been shown to bind to lipid membranes with binding influenced by both membrane composition and pH. Aside from the misfolding events associated with prion pathogenesis, PrP can...

journal_title：Biophysical journal

authors： Le Brun AP,Haigh CL,Drew SC,James M,Boland MP,Collins SJ

更新日期：2014-11-18 00:00:00

abstract：:The biological influence of radiation on living matter has been studied for years; however, several questions about the detailed mechanism of radiation damage formation remain largely unanswered. Among all biomolecules exposed to radiation, DNA plays an important role because any damage to its molecular structure can ...

journal_title：Biophysical journal

authors： Czapla-Masztafiak J,Szlachetko J,Milne CJ,Lipiec E,Sá J,Penfold TJ,Huthwelker T,Borca C,Abela R,Kwiatek WM

更新日期：2016-03-29 00:00:00

abstract：:Recently a periplasmic glucose/galactose binding protein, GGRQ26C, immobilized on a gold surface has been used as an active part of a glucose biosensor based on quartz microbalance technique. However the nature of the glucose detection was not clear. Here we have found that the receptor protein film immobilized on the...

journal_title：Biophysical journal

authors： Sokolov I,Subba-Rao V,Luck LA

更新日期：2006-02-01 00:00:00

abstract：:Computer simulations suggest that the translocation of arginine through the hydrocarbon core of a lipid membrane proceeds by the formation of a water-filled defect that keeps the arginine molecule hydrated even at the center of the bilayer. We show here that adding additional arginine molecules into one of these water...

journal_title：Biophysical journal

authors： MacCallum JL,Bennett WF,Tieleman DP

更新日期：2011-07-06 00:00:00

abstract：:The crowded environment of cells poses a challenge for rapid protein-protein association. Yet, it has been established that the rates of association are similar in crowded and in dilute solutions. Here we probe the pathway leading to fast association between TEM1 β-lactamase and its inhibitor protein BLIP in crowded s...

journal_title：Biophysical journal

authors： Phillip Y,Harel M,Khait R,Qin S,Zhou HX,Schreiber G

更新日期：2012-09-05 00:00:00

abstract：:The aim of this study was to gain insight into how interactions between proteins and lipids in membranes are sensed at the protein-lipid interface. As a probe to analyze this interface, we used deuterium-labeled acyl chains that were covalently linked to a model transmembrane peptide. First, a perdeuterated palmitoyl ...

journal_title：Biophysical journal

authors： Nyholm TK,van Duyl B,Rijkers DT,Liskamp RM,Killian JA

更新日期：2011-10-19 00:00:00

abstract：:Recent studies have demonstrated that caffeine can act as an antimutagen and inhibit the cytoxic and/or cytostatic effects of some DNA intercalating agents. It has been suggested that this inhibitory effect may be due to complexation of the DNA intercalator with caffeine. In this study we employ optical absorption, fl...

journal_title：Biophysical journal

authors： Larsen RW,Jasuja R,Hetzler RK,Muraoka PT,Andrada VG,Jameson DM

更新日期：1996-01-01 00:00:00

abstract：:Intracellular transport of cargos along microtubules is often complicated by the topology of the underlying filament network. The fundamental building blocks for this complex arrangement are filament intersections. The navigation of cargos across microtubule intersections remains poorly understood. Here, we demonstrat...

journal_title：Biophysical journal

authors： Osunbayo O,Butterfield J,Bergman J,Mershon L,Rodionov V,Vershinin M

更新日期：2015-03-24 00:00:00

abstract：:Small amounts of paramagnetic cations quench the phosphorescence of DNA. Although the emission intensity is monotonic with increasing thymine content, the quenching efficiency is not. The cations quench from phosphate sites. ...

journal_title：Biophysical journal

authors： Isenberg I,Rosenbluth R,Baird SL Jr

更新日期：1967-07-01 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00

abstract：:A new experimental approach has been developed to study the distribution of local electrostatic potential around specific protons in biologically important molecules. The approach is the development of a method denoted as "spin label/spin probe," which was proposed by one of us (. Mol. Biol. 6:498-507). The proposed m...

journal_title：Biophysical journal

authors： Likhtenshtein GI,Adin I,Novoselsky A,Shames A,Vaisbuch I,Glaser R

更新日期：1999-07-01 00:00:00

abstract：:Receptor activity modifying proteins (RAMPs) are a family of single-pass transmembrane proteins that dimerize with G-protein-coupled receptors. They may alter the ligand recognition properties of the receptors (particularly for the calcitonin receptor-like receptor, CLR). Very little structural information is availabl...

journal_title：Biophysical journal

authors： Simms J,Hay DL,Wheatley M,Poyner DR

更新日期：2006-07-15 00:00:00

abstract：:A microscopic model of passive transverse mass transport of small solutes in the viable epidermal layer of human skin is formulated on the basis of a hexagonal array of cells (i.e., keratinocytes) bounded by 4-nm-thick, anisotropic lipid bilayers and separated by 1-μm layers of extracellular fluid. Gap junctions and t...

journal_title：Biophysical journal

authors： Nitsche JM,Kasting GB

更新日期：2013-05-21 00:00:00

abstract：:In an attempt to better define the parameters governing reconstitution and two-dimensional crystallization of membrane proteins, we have studied Ca2(+)-ATPase from rabbit sarcoplasmic reticulum. This ion pump forms vanadate-induced crystals in its native membrane and has previously been reconstituted at high lipid-to-...

journal_title：Biophysical journal

authors： Young HS,Rigaud JL,Lacapère JJ,Reddy LG,Stokes DL

更新日期：1997-06-01 00:00:00

abstract：:The study of mechanotransduction relies on tools that are capable of applying mechanical forces to elicit and assess cellular responses. Here we report a new (to our knowledge) technique, called two-bubble acoustic tweezing cytometry (TB-ATC), for generating spatiotemporally controlled subcellular mechanical forces on...

journal_title：Biophysical journal

authors： Chen D,Sun Y,Gudur MS,Hsiao YS,Wu Z,Fu J,Deng CX

更新日期：2015-01-06 00:00:00

abstract：:The mitochondrial channel, VDAC, forms large (3 nm in diameter) aqueous pores through membranes. We measured ATP flow (using the luciferin/luciferase method) through these channels after reconstitution into planar phospholipid membranes. In the open state of VDAC, as many as 2 x 10(6) ATP molecules can flow through on...

journal_title：Biophysical journal

authors： Rostovtseva T,Colombini M

更新日期：1997-05-01 00:00:00

abstract：:Microbes inhabiting Earth have adapted to diverse environments of water, air, soil, and often at the interfaces of multiple media. In this study, we focus on the behavior of Caulobacter crescentus, a singly flagellated bacterium, at the air/water interface. Forward swimming C. crescentus swarmer cells tend to get phys...

journal_title：Biophysical journal

authors： Morse M,Huang A,Li G,Maxey MR,Tang JX

更新日期：2013-07-02 00:00:00

abstract：:The E5 oncoprotein is the major transforming protein of bovine papillomavirus type 1. This 44-residue transmembrane protein can interact with the platelet-derived growth factor receptor β, leading to ligand-independent activation and cell transformation. For productive interaction, E5 needs to dimerize via a C-termina...

journal_title：Biophysical journal

authors： Windisch D,Hoffmann S,Afonin S,Vollmer S,Benamira S,Langer B,Bürck J,Muhle-Goll C,Ulrich AS

更新日期：2010-09-22 00:00:00

abstract：:Two mechanisms have been proposed to account for solute permeation of lipid bilayers. Partitioning into the hydrophobic phase of the bilayer, followed by diffusion, is accepted by many for the permeation of water and other small neutral solutes, but transient pores have also been proposed to account for both water and...

journal_title：Biophysical journal

authors： Paula S,Volkov AG,Van Hoek AN,Haines TH,Deamer DW

更新日期：1996-01-01 00:00:00

abstract：:In the budding yeast nucleus, transfer RNA (tRNA) genes are considered to localize in the vicinity of the nucleolus; however, the use of Hi-C and fluorescent repressor-operator system techniques has clearly indicated that the tRNA genes are distributed not only around the nucleolus but also at other nuclear locations....

journal_title：Biophysical journal

authors： Tokuda N

更新日期：2020-05-05 00:00:00

abstract：:Here we present a model for maltodextrin translocation through maltoporin channels. In a first step, our theoretical analysis does consider the case of a single binding site for a given substrate in a structurally unaffected channel with a possibly different entrance barrier on either side. It is shown how by means of...

journal_title：Biophysical journal

authors： Schwarz G,Danelon C,Winterhalter M

更新日期：2003-05-01 00:00:00

abstract：:In this review, we focus on the early events in the process of fibroblast spreading on fibronectin matrices of different rigidities. We present a focused position piece that illustrates the many different tests that a cell makes of its environment before it establishes mature matrix adhesions. When a fibroblast is pla...

journal_title：Biophysical journal

authors： Wolfenson H,Iskratsch T,Sheetz MP

更新日期：2014-12-02 00:00:00

abstract：:We measured the influence of alternative versions of the Drosophila melanogaster myosin heavy chain relay domain on muscle mechanical properties. We exchanged relay domain regions (encoded by alternative versions of exon 9) between an embryonic (EMB) isoform and the indirect flight muscle isoform (IFI) of myosin. Prev...

journal_title：Biophysical journal

authors： Yang C,Ramanath S,Kronert WA,Bernstein SI,Maughan DW,Swank DM

更新日期：2008-12-01 00:00:00