Transfer of arginine into lipid bilayers is nonadditive.

abstract：:The solutions to a general class of axon partial differential equations proposed by FitzHugh which includes the Hodgkin-Huxley equations are studied. It is shown that solutions to the partial differential equations are exactly the solutions to a related set of integral equations. An iterative procedure for constructin...

journal_title：Biophysical journal

authors： Evans J,Shenk N

更新日期：1970-11-01 00:00:00

abstract：:The main goal of this study is to investigate the role of mitochondrial [Ca(2+)], [Ca(2+)](m), in the possible up-regulation of the NADH production rate during increased workload. Such up-regulation is necessary to support increased flux through the electron transport chain and increased ATP synthesis rates. Intact ca...

journal_title：Biophysical journal

authors： Brandes R,Bers DM

更新日期：2002-08-01 00:00:00

abstract：:Neurons communicate by releasing neurotransmitters that are stored in intracellular vesicular compartments. PC12 cells are frequently used as a model secretory cell line that is described to have two subpools of vesicles: small clear vesicles and dense core vesicles. We measured transmitter molecules released from ves...

journal_title：Biophysical journal

authors： Adams RD,Harkins AB

更新日期：2014-12-16 00:00:00

abstract：:In wild-type lenses from various species, an intracellular hydrostatic pressure gradient goes from ∼340 mmHg in central fiber cells to 0 mmHg in surface cells. This gradient drives a center-to-surface flow of intracellular fluid. In lenses in which gap-junction coupling is increased, the central pressure is lower, whe...

journal_title：Biophysical journal

authors： Gao J,Sun X,White TW,Delamere NA,Mathias RT

更新日期：2015-11-03 00:00:00

abstract：:For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating ...

journal_title：Biophysical journal

authors： Hustedt EJ,Smirnov AI,Laub CF,Cobb CE,Beth AH

更新日期：1997-04-01 00:00:00

abstract：:Guanine-rich DNA repeat sequences located at the terminal ends of chromosomal DNA can fold in a sequence-dependent manner into G-quadruplex structures, notably the terminal 150-200 nucleotides at the 3' end, which occur as a single-stranded DNA overhang. The crystal structures of quadruplexes with two and four human t...

journal_title：Biophysical journal

authors： Haider S,Parkinson GN,Neidle S

更新日期：2008-07-01 00:00:00

abstract：:A recent thermodynamic model describes a reversible reaction between cholesterol (C) and phospholipid (P) to form a condensed complex C(nq)P(np). Here q and p are relatively prime integers used to define the stoichiometric composition, and n is a measure of cooperativity. The present study applies this model to the sc...

journal_title：Biophysical journal

authors： Anderson TG,McConnell HM

更新日期：2001-11-01 00:00:00

abstract：:Heart surface optical mapping of transmembrane potentials has been widely used in studies of normal and pathological heart rhythms and defibrillation. In these studies, three-dimensional spatio-temporal events can only be inferred from two-dimensional surface potential maps. We present a novel optical system that enab...

journal_title：Biophysical journal

authors： Hooks DA,LeGrice IJ,Harvey JD,Smaill BH

更新日期：2001-11-01 00:00:00

abstract：:Green flagellates possess rhodopsin-like photoreceptors involved in control of their behavior via generation of photocurrents across the plasma membrane. Chlamydomonas mutants blocked in retinal biosynthesis are "blind," but they can be rescued by the addition of exogenous retinoids. Photosignaling by chlamyrhodopsin ...

journal_title：Biophysical journal

authors： Govorunova EG,Sineshchekov OA,Gärtner W,Chunaev AS,Hegemann P

更新日期：2001-11-01 00:00:00

abstract：:The fusion peptides of HIV and influenza virus are crucial for viral entry into a host cell. We report the membrane-perturbing and structural properties of fusion peptides from the HA fusion protein of influenza virus and the gp41 fusion protein of HIV. Our goals were to determine: 1), how fusion peptides alter struct...

journal_title：Biophysical journal

authors： Haque ME,Koppaka V,Axelsen PH,Lentz BR

更新日期：2005-11-01 00:00:00

abstract：:Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These resu...

journal_title：Biophysical journal

authors： Sikorav JL,Pelta J,Livolant F

更新日期：1994-10-01 00:00:00

abstract：:Lateral tension in cell plasma membranes plays an essential role in regulation of a number of membrane-related intracellular processes and cell motion. Understanding the physical factors generating the lateral tension and quantitative determination of the tension distribution along the cell membrane is an emerging top...

journal_title：Biophysical journal

authors： Schweitzer Y,Lieber AD,Keren K,Kozlov MM

更新日期：2014-01-07 00:00:00

abstract：:We accelerate protein folding in all-atom molecular dynamics simulations by introducing alternating hydrogen bond potentials as a supplement to the force field. The alternating hydrogen bond potentials result in accelerated hydrogen bond reordering, which leads to rapid formation of secondary structure elements. The m...

journal_title：Biophysical journal

authors： Wolf MG,de Leeuw SW

更新日期：2008-05-15 00:00:00

abstract：:We present a theory for proton diffusion through an immobilized protein membrane perfused with an electrolyte and a buffer. Using a Nernst-Planck equation for each species and assuming local charge neutrality, we obtain two coupled nonlinear diffusion equations with new diffusion coefficients dependent on the concentr...

journal_title：Biophysical journal

authors： Zabusky NJ,Deem GS

更新日期：1979-01-01 00:00:00

abstract：:Members of the epidermal growth factor receptor family play important roles in various cellular processes, both in physiological and in pathological conditions. Dimerization and autophosphorylation of these receptor tyrosine kinases are key events of signal transduction. Details of the molecular events of the signalin...

journal_title：Biophysical journal

authors： Bagossi P,Horváth G,Vereb G,Szöllösi J,Tözsér J

更新日期：2005-02-01 00:00:00

abstract：:Hemoglobin functions as a tetrameric oxygen transport protein, with each subunit containing a heme cofactor. Its denaturation, either in vivo or in vitro, involves autoxidation to methemoglobin, followed by cofactor loss and globin unfolding. We have proposed a global disassembly scheme for human methemoglobin, linkin...

journal_title：Biophysical journal

authors： Samuel PP,White MA,Ou WC,Case DA,Phillips GN Jr,Olson JS

更新日期：2020-03-24 00:00:00

abstract：:Enzyme function depends on specific conformational motions. We show that the temperature dependence of enzyme kinetic parameters can provide insight into these functionally relevant motions. While investigating the catalytic properties of IPMDH from Escherichia coli, we found that its catalytic efficiency (k(cat)/K(M,...

journal_title：Biophysical journal

authors： Hajdú I,Szilágyi A,Kardos J,Závodszky P

更新日期：2009-06-17 00:00:00

abstract：:A simple formula for the expansion rate U of the bilayer membrane perimeter was derived by the solution of the hydrodynamic and the Laplace equations at small rates and small contact angles: U = theta delta/6 micron ln (h/e 3/2 h0), where theta is the equilibrium contact angle between the bilayer membrane and the Plat...

journal_title：Biophysical journal

authors： Dimitrov DS

更新日期：1981-10-01 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:Films of O-phospho-L-serine-P-ethylester (PSE) were studied by infrared spectroscopy. PSE films were studied pure and as 1:1 mixture with LiOH, NaOH, KOH, and Ca(OH)2 as a function of the degree of hydration. The same investigations were performed if (L-glu)n was added to the system (ratio 1:1, PSE/glu residue). In th...

journal_title：Biophysical journal

authors： Leberle K,Kempf I,Zundel G

更新日期：1989-04-01 00:00:00

abstract：:A 3D model was developed and used to explore dynamics of phosphatidylinositol-4,5-bisphosphate (PIP2) signaling in cerebellar Purkinje neurons. Long-term depression in Purkinje neurons depends on coincidence detection of climbing fiber stimulus evoking extracellular calcium flux into the cell and parallel fiber stimul...

journal_title：Biophysical journal

authors： Brown SA,Morgan F,Watras J,Loew LM

更新日期：2008-08-01 00:00:00

abstract：:Oriented solid-state NMR in combination with multiple-residue-specific (15)N labeling and extensive numerical spectral analysis is proposed to determine helix conformations of large membrane proteins in native membranes. The method is demonstrated on uniaxially oriented samples of (15)N-methionine, -valine, and -glyci...

journal_title：Biophysical journal

authors： Vosegaard T,Kamihira-Ishijima M,Watts A,Nielsen NC

更新日期：2008-01-01 00:00:00

abstract：:The initial output of a time-resolved macromolecular crystallography experiment is a time-dependent series of difference electron density maps that displays the time-dependent changes in underlying structure as a reaction progresses. The goal is to interpret such data in terms of a small number of crystallographically...

journal_title：Biophysical journal

authors： Kostov KS,Moffat K

更新日期：2011-01-19 00:00:00

abstract：:Total internal reflection excitation used in combination with fluorescence correlation spectroscopy (TIR-FCS) is a method for characterizing the dynamic behavior and absolute concentrations of fluorescent molecules near or at the interface of a planar substrate and a solution. In this work, we demonstrate for the firs...

journal_title：Biophysical journal

authors： Lieto AM,Cush RC,Thompson NL

更新日期：2003-11-01 00:00:00

abstract：:Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resol...

journal_title：Biophysical journal

authors： Gustafsson MG,Shao L,Carlton PM,Wang CJ,Golubovskaya IN,Cande WZ,Agard DA,Sedat JW

更新日期：2008-06-01 00:00:00

abstract：:The physical characteristics which govern the water relations of the giant-celled sporangiophore of Phycomyces blakesleeanus were measured with the pressure probe technique and with nanoliter osmometry. These properties are important because they govern water uptake associated with cell growth and because they may in...

journal_title：Biophysical journal

authors： Cosgrove DJ,Ortega JK,Shropshire W Jr

更新日期：1987-03-01 00:00:00

abstract：:A challenge in the understanding of creatine kinase (CK) fluxes reflected by NMR magnetization transfer in the perfused rat heart is the choice of a kinetic model of analysis. The complexity of the energetic pathways, due to the presence of adenosine triphosphate (ATP)-inorganic phosphate (Pi) exchange, of metabolite ...

journal_title：Biophysical journal

authors： Joubert F,Hoerter JA,Mazet JL

更新日期：2001-12-01 00:00:00

abstract：:Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditio...

journal_title：Biophysical journal

authors： Bédard C,Destexhe A

更新日期：2008-02-15 00:00:00

abstract：:Crystallographic studies of insulin-protamine complexes, such as neutral protamine Hagedorn (NPH) insulin, have been hampered by high crystal solvent content, small crystal dimensions, and extensive disorder in the protamine molecules. We report herein in situ tapping mode atomic force microscopy (TMAFM) studies of cr...

journal_title：Biophysical journal

authors： Yip CM,Brader ML,Frank BH,DeFelippis MR,Ward MD

更新日期：2000-01-01 00:00:00

abstract：:The circadian clocks keeping time in many living organisms rely on self-sustained biochemical oscillations entrained by external cues, such as light, to the 24-h cycle induced by Earth's rotation. However, environmental cues are unreliable due to the variability of habitats, weather conditions, or cue-sensing mechanis...

journal_title：Biophysical journal

authors： Pfeuty B,Thommen Q,Lefranc M

更新日期：2011-06-08 00:00:00