A liquid crystalline phase in spermidine-condensed DNA.

Abstract:

:Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These results are discussed according to Flory's theory for the ordering of rigid polymers. The liquid crystal described here corresponds to an ordering in the presence of attractive interactions, in contrast with classical liquid crystalline DNA. Polyamines are often used in vitro to study the functional properties of DNA. We suggest that the existence of a liquid crystalline state in spermidine-condensed DNA is relevant to these studies.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Sikorav JL,Pelta J,Livolant F

doi

10.1016/S0006-3495(94)80640-X

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

1387-92

issue

4

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(94)80640-X

journal_volume

67

pub_type

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