Structural and functional features of one- and two-headed biotinated kinesin derivatives.

abstract：:The sorting of lipids and proteins in cellular trafficking pathways is a process of central importance in maintaining compartmentalization in eukaryotic cells. However, the mechanisms behind these sorting phenomena are currently far from being understood. Among several mechanistic suggestions, membrane curvature has b...

journal_title：Biophysical journal

authors： Tian A,Baumgart T

更新日期：2009-04-08 00:00:00

abstract：:Air dried films of purple membranes (PM) from Halobacterium halobium containing the photochromic protein bacteriorhodopsin (BR) were prepared and the BR-photocycle of this material analyzed. The absorption maxima of the initial state B (lambda(max) = 570 nm) and the photochemical intermediate M (lambda(max) = 412 nm),...

journal_title：Biophysical journal

authors： Hampp N,Bräuchle C,Oesterhelt D

更新日期：1990-07-01 00:00:00

abstract：:Amperometry recording reveals the exocytosis of catecholamine from individual vesicles as a sequential process, typically beginning slowly with a prespike foot, accelerating sharply to initiate a spike, reaching a peak, and then decaying. This complex sequence reflects the interplay between diffusion, flux through a f...

journal_title：Biophysical journal

authors： Jackson MB,Hsiao YT,Chang CW

更新日期：2020-07-07 00:00:00

abstract：:The lipid matrix present in the uppermost layer of the skin, the stratum corneum, plays a crucial role in the skin barrier function. The lipids are organized into two lamellar phases. To gain more insight into the molecular organization of one of these lamellar phases, we performed neutron diffraction studies. In the ...

journal_title：Biophysical journal

authors： Groen D,Gooris GS,Barlow DJ,Lawrence MJ,van Mechelen JB,Demé B,Bouwstra JA

更新日期：2011-03-16 00:00:00

abstract：:Many studies of specific protein-nucleic acid binding use short oligonucleotides or restriction fragments, in part to minimize the potential for nonspecific binding of the protein. However, when the specificity ratio is low, multiple nonspecifically bound proteins may occupy the region of DNA corresponding to one spec...

journal_title：Biophysical journal

authors： Tsodikov OV,Holbrook JA,Shkel IA,Record MT Jr

更新日期：2001-10-01 00:00:00

abstract：:We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The in...

journal_title：Biophysical journal

authors： Friedrich M,Gan Q,Ermolayev V,Harms GS

更新日期：2011-04-20 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:Single-molecule force spectroscopy is used to probe the kinetics of receptor-ligand bonds by applying mechanical forces to an intermediate media on which the molecules reside. When this intermediate media is a live cell, the viscoelastic properties can affect the calculation of rate constants. We theoretically investi...

journal_title：Biophysical journal

authors： Gupta VK,Neeves KB,Eggleton CD

更新日期：2012-07-03 00:00:00

abstract：:Mutant Ras proteins are important drivers of human cancers, yet no approved drugs act directly on this difficult target. Over the last decade, the idea has emerged that oncogenic signaling can be diminished by molecules that drive Ras into orientations in which effector-binding interfaces are occluded by the cell memb...

journal_title：Biophysical journal

authors： Neale C,García AE

更新日期：2020-03-10 00:00:00

abstract：:Biological cells embedded in fibrous matrices have been observed to form intercellular bands of dense and aligned fibers through which they mechanically interact over long distances. Such matrix-mediated cellular interactions have been shown to regulate various biological processes. This study aimed to explore the eff...

journal_title：Biophysical journal

authors： Sopher RS,Tokash H,Natan S,Sharabi M,Shelah O,Tchaicheeyan O,Lesman A

更新日期：2018-10-02 00:00:00

abstract：:The effect of aggregation of gramicidin A' (GA) on the phase structure of dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles was studied by cw-ESR using a chain-labeled lipid (16PC) at temperatures between 30 degrees and 45 degreesC that span the main phase transition of DPPC. Boundary lipids were observed o...

journal_title：Biophysical journal

authors： Ge M,Freed JH

更新日期：1999-01-01 00:00:00

abstract：:Forces between type I collagen triple helices are studied in solvents of varying hydrogen-bonding ability. The swelling of collagen fibers in reconstituted films is controlled by the concentration of soluble polymers that are excluded from the fibers and that compete osmotically with collagen for available solvent. Th...

journal_title：Biophysical journal

authors： Kuznetsova N,Rau DC,Parsegian VA,Leikin S

更新日期：1997-01-01 00:00:00

abstract：:The primary drivers of yeast endocytosis are actin polymerization and curvature-generating proteins, such as clathrin and BAR domain proteins. Previous work has indicated that these factors may not be capable of generating the forces necessary to overcome turgor pressure. Thus local reduction of the turgor pressure, v...

journal_title：Biophysical journal

authors： Scher-Zagier JK,Carlsson AE

更新日期：2016-12-20 00:00:00

abstract：:The UV-visible absorption and magnetic circular dichroism (MCD) spectra of the ferric, ferrous, CO-ligated forms and kinetic photolysis intermediates of the tetraheme electron-transfer protein cytochrome c3 (Cc3) are reported. Consistent with bis-histidinyl axial coordination of the hemes in this Class III c-type cyto...

journal_title：Biophysical journal

authors： O'Connor DB,Goldbeck RA,Hazzard JH,Kliger DS,Cusanovich MA

更新日期：1993-10-01 00:00:00

abstract：:The kinetics of oxygen geminate binding with the taxonomic substates of MbO2 are reported. The maximum entropy method was used to analyze the rebinding kinetics of MbCO and MbO2 monitored in the Soret. The resulting rate distributions were found to consist of a small number of overlapping bands. A global parametric fi...

journal_title：Biophysical journal

authors： Tetreau C,Novikov E,Tourbez M,Lavalette D

更新日期：2002-04-01 00:00:00

abstract：:The molecular cascade that controls switching of the direction of rotation of Escherichia coli flagellar motors is well known, but the conformational changes that allow the rotor to switch are still unclear. The signaling molecule CheY, when phosphorylated, binds to the C-ring at the base of the rotor, raising the pro...

journal_title：Biophysical journal

authors： Hosu BG,Berg HC

更新日期：2018-02-06 00:00:00

abstract：:We explore the means by which immobilization of a substrate on a surface can increase the rate of a diffusion-controlled enzymatic reaction. A quasichemical approach is developed and compared with Brownian dynamics simulations. We use these methods to show that restricting only the orientation of the enzyme by long-ra...

journal_title：Biophysical journal

authors： Nag A,Dinner AR

更新日期：2006-02-01 00:00:00

abstract：:The diffusion of plastoquinol and its binding to the cytochrome bf complex, which occurs during linear photosynthetic electron transport and is analogous to reaction sequences found in most energy-converting membranes, has been studied in intact thylakoid membranes. The flash-induced electron transfer between the late...

journal_title：Biophysical journal

authors： Mitchell R,Spillmann A,Haehnel W

更新日期：1990-10-01 00:00:00

abstract：:Pre-mRNA processing protein 40 (Prp40) is a nuclear protein that has a role in pre-mRNA splicing. Prp40 possesses two leucine-rich nuclear export signals, but little is known about the function of Prp40 in the export process. Another protein that has a role in protein export is centrin, a member of the EF-hand superfa...

journal_title：Biophysical journal

authors： Díaz Casas A,Chazin WJ,Pastrana-Ríos B

更新日期：2017-06-20 00:00:00

abstract：:We investigate the validity of continuum electrostatics in the gramicidin A channel using a recently determined high-resolution structure. The potential and electric field acting on ions in and around the channel are computed by solving Poisson's equation. These are then used in Brownian dynamics simulations to obtain...

journal_title：Biophysical journal

authors： Edwards S,Corry B,Kuyucak S,Chung SH

更新日期：2002-09-01 00:00:00

abstract：:Integrin-mediated signaling is crucial for cell-substrate adhesion and can be triggered from both intra- and extracellular interactions. Although talin binding is sufficient for inside-out activation of integrin, other cytoplasmic proteins such as α-actinin and filamin can directly interfere with talin-mediated integr...

journal_title：Biophysical journal

authors： Shams H,Mofrad MRK

更新日期：2017-08-22 00:00:00

abstract：:T cell receptor phosphorylation by Lck is an essential step in T cell activation. It is known that the conformational states of Lck control enzymatic activity; however, the underlying principles of how Lck finds its substrate over the plasma membrane remain elusive. Here, single-particle tracking is paired with photoa...

journal_title：Biophysical journal

authors： Hilzenrat G,Pandžić E,Yang Z,Nieves DJ,Goyette J,Rossy J,Ma Y,Gaus K

更新日期：2020-03-24 00:00:00

abstract：:Guanine-rich DNA repeat sequences located at the terminal ends of chromosomal DNA can fold in a sequence-dependent manner into G-quadruplex structures, notably the terminal 150-200 nucleotides at the 3' end, which occur as a single-stranded DNA overhang. The crystal structures of quadruplexes with two and four human t...

journal_title：Biophysical journal

authors： Haider S,Parkinson GN,Neidle S

更新日期：2008-07-01 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:The interaction between protein molecules and the hydroxyapatite (HAP) crystal is an important research topic in many fields. However, the nature of their noncovalent bonding is still not clear at the atomic level. In this work, molecular dynamics simulation, steered molecular dynamics simulation, and quantum chemistr...

journal_title：Biophysical journal

authors： Dong X,Wang Q,Wu T,Pan H

更新日期：2007-08-01 00:00:00

abstract：:The photoelectron quantum yields of 21 common amino acids and 15 polyamino acids were measured in the 180-240 nm wavelength region. On the average, the quantum yields of these two groups exhibit quite similar wavelength dependence. For lambda > 220 nm all amino acid and polyamino acid quantum yields are

journal_title：Biophysical journal

authors： Dam RJ,Burke CA,Griffith OH

更新日期：1974-06-01 00:00:00

abstract：:The piezoelectric properties of single collagen type I fibrils in fascia were imaged with sub-20 nm spatial resolution using piezoresponse force microscopy. A detailed analysis of the piezoresponse force microscopy signal in controlled tip-fibril geometry revealed shear piezoelectricity parallel to the fibril axis. Th...

journal_title：Biophysical journal

authors： Harnagea C,Vallières M,Pfeffer CP,Wu D,Olsen BR,Pignolet A,Légaré F,Gruverman A

更新日期：2010-06-16 00:00:00

abstract：:39K nuclear magnetic resonance (NMR) spectra were readily obtained, in vivo, from rat muscle, kidney, and brain in 5-10 min with signal-to-noise ratios of approximately 20:1. Quantitation of the K+ signal was achieved by reference to an external standard of KCl/dysprosium nitrate as well as by reference to the proton ...

journal_title：Biophysical journal

authors： Adam WR,Koretsky AP,Weiner MW

更新日期：1987-02-01 00:00:00

abstract：:Whether proteins or lipids are the primary target sites for general anesthetic action has engendered considerable debate. Recent in vivo studies have shown that the S(+) and R(-) enantiomers of isoflurane are not equipotent, implying involvement of proteins. Bovine serum albumin (BSA), a soluble protein devoid of lipi...

journal_title：Biophysical journal

authors： Xu Y,Tang P,Firestone L,Zhang TT

更新日期：1996-01-01 00:00:00

abstract：:Xenopus oocytes express mechanosensitive (MS(XO)) channels that can be studied in excised patches of membrane with the patch-clamp technique. This study examines the steady-state kinetic gating properties of MS(XO) channels using detailed single-channel analysis. The open and closed one-dimensional dwell-time distribu...

journal_title：Biophysical journal

authors： Gil Z,Magleby KL,Silberberg SD

更新日期：2001-10-01 00:00:00