Abstract:
:We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 μm deep penetration into biological tissue.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Friedrich M,Gan Q,Ermolayev V,Harms GSdoi
10.1016/j.bpj.2010.12.3748subject
Has Abstractpub_date
2011-04-20 00:00:00pages
L43-5issue
8eissn
0006-3495issn
1542-0086pii
S0006-3495(11)00303-1journal_volume
100pub_type
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