Abstract:
:In this work, boundary element modeling is used to study the transport of highly charged rod-like model polyions of various length under a variety of different aqueous salt conditions. Transport properties considered include free solution electrophoretic mobility, translational diffusion, and the components of the "tether force" tensor. The model parameters are chosen to coincide with transport measurements of duplex DNA carried out under six different salt/temperature conditions. The focus of the analysis is on the length dependence of the free solution electrophoretic mobility. In a solution containing 0.04 M Tris-acetate buffer at 25 degrees C, calculated mobilities using straight rod models show a stronger dependence on fragment length than that observed experimentally. By carrying out model studies on curved rod models, it is concluded that the "leveling off" of mobility with fragment length is due, in part at least, to the finite curvature of DNA. Experimental mobilities of long duplex DNA in monovalent alkali salts are reasonably well explained once account is taken of long-range bending and the simplifying assumptions of the model studies.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Allison S,Chen C,Stigter Ddoi
10.1016/S0006-3495(01)75900-0subject
Has Abstractpub_date
2001-11-01 00:00:00pages
2558-68issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(01)75900-0journal_volume
81pub_type
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