Temperature, stability, and the hydrophobic interaction.

abstract：:We used micron-sized latex spheres to probe the phase state and the viscoelastic properties of dimyristoylphosphatidylcholine (DMPC) bilayers as a function of temperature. One or two particles were manipulated and stuck to a DMPC giant vesicle by means of an optical trap. Above the fluid-gel main transition temperatur...

journal_title：Biophysical journal

authors： Dimova R,Pouligny B,Dietrich C

更新日期：2000-07-01 00:00:00

abstract：:A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

journal_title：Biophysical journal

authors： Schwartz TL,Kado RT

更新日期：1977-06-01 00:00:00

abstract：:Specific proteins and lipids sequester to regions of cell membranes called rafts. Due to their high content of sphingomyelin (SM) and cholesterol, raft bilayers are thicker than nonraft bilayers and, at least at 4 degrees C, are resistant to Triton X-100 extraction. It has been postulated that rafts concentrate protei...

journal_title：Biophysical journal

authors： McIntosh TJ,Vidal A,Simon SA

更新日期：2003-09-01 00:00:00

abstract：:Guanine-rich DNA repeat sequences located at the terminal ends of chromosomal DNA can fold in a sequence-dependent manner into G-quadruplex structures, notably the terminal 150-200 nucleotides at the 3' end, which occur as a single-stranded DNA overhang. The crystal structures of quadruplexes with two and four human t...

journal_title：Biophysical journal

authors： Haider S,Parkinson GN,Neidle S

更新日期：2008-07-01 00:00:00

abstract：:A new method for the investigation of ion translocating membrane proteins is presented. Protein containing membrane fragments or vesicles are adsorbed to a solid supported membrane. The solid supported membrane consists of a lipid monolayer on a gold evaporated or gold sputtered glass substrate which is coated with a ...

journal_title：Biophysical journal

authors： Seifert K,Fendler K,Bamberg E

更新日期：1993-02-01 00:00:00

abstract：:This paper describes an analysis of microscopic models for the coupling between ion flow and rotation of bacterial flagella. In model I it is assumed that intersecting half-channels exist on the rotor and the stator and that the driving ion is constrained to move together with the intersection site. Model II is based ...

journal_title：Biophysical journal

authors： Läuger P

更新日期：1988-01-01 00:00:00

abstract：:Multiple molecular dynamics simulations of bacterioopsin pulling from its C-terminus show that its alpha-helices unfold individually. In the first metastable state observed in the simulations, helix G is unfolded at its C-terminal segment while the rest of helix G (residues 200-216) is folded and opposes resistance be...

journal_title：Biophysical journal

authors： Seeber M,Fanelli F,Paci E,Caflisch A

更新日期：2006-11-01 00:00:00

abstract：:Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

journal_title：Biophysical journal

authors： De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

更新日期：2010-09-22 00:00:00

abstract：:The lipid matrix, or the lipid bilayer, of cell membranes is a natural binding site for amphipathic molecules, including antimicrobial peptides, pore-forming proteins, and many drugs. The unique property of pore-forming antimicrobial peptides is that they exhibit a threshold concentration (called the lethal concentrat...

journal_title：Biophysical journal

authors： Huang HW

更新日期：2009-04-22 00:00:00

abstract：:A method for fabricating biomimetic surfaces from intact cell membranes is described. A monolayer of alkanethiol on gold is covered by a second layer derived from the components of erythrocyte membranes either by self-assembly or by Langmuir-Blodgett methods. The resulting asymmetric hybrid layer was characterized by ...

journal_title：Biophysical journal

authors： Rao NM,Plant AL,Silin V,Wight S,Hui SW

更新日期：1997-12-01 00:00:00

abstract：:In order to verify the "radiation protection" effect of prosthetic groups in irradiated conjugated proteins and the influence of their electronic structure on intramolecular excitation transfer, two series of substances were examined. The first was composed of ferrihemoglobin and its constituents and the second was co...

journal_title：Biophysical journal

authors： Crippa PR,Vecli A

更新日期：1970-03-01 00:00:00

abstract：:We accelerate protein folding in all-atom molecular dynamics simulations by introducing alternating hydrogen bond potentials as a supplement to the force field. The alternating hydrogen bond potentials result in accelerated hydrogen bond reordering, which leads to rapid formation of secondary structure elements. The m...

journal_title：Biophysical journal

authors： Wolf MG,de Leeuw SW

更新日期：2008-05-15 00:00:00

abstract：:Myoglobin (Mb) is a model system for ligand binding and migration. The energy barriers (ΔG) for ligand migration in Mb have been studied in the past by experiment and theory and significant differences between different approaches were found. From experiment, it is known that Mb can assume a large number of conformati...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2012-01-18 00:00:00

abstract：:In some fungi, a premeiotic process known as repeat-induced point mutation (RIP) can accurately identify and mutate nearly all gene-sized DNA repeats present in the haploid germline nuclei. Studies in Neurospora crassa have suggested that RIP detects sequence homology directly between intact DNA double helices, withou...

journal_title：Biophysical journal

authors： Mazur AK,Gladyshev E

更新日期：2018-08-21 00:00:00

abstract：:Proteins are denatured in aqueous urea solution. The nature of the molecular driving forces has received substantial attention in the past, whereas the question how urea acts at different phases of unfolding is not yet well understood at the atomic level. In particular, it is unclear whether urea actively attacks fold...

journal_title：Biophysical journal

authors： Stumpe MC,Grubmüller H

更新日期：2009-05-06 00:00:00

abstract：:Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical prop...

journal_title：Biophysical journal

authors： Yurlova L,Kahya N,Aggarwal S,Kaiser HJ,Chiantia S,Bakhti M,Pewzner-Jung Y,Ben-David O,Futerman AH,Brügger B,Simons M

更新日期：2011-12-07 00:00:00

abstract：:The mathematics of the binomial model for quantal neurotransmitter release is considered in general terms, to explore what information might be extractable from statistical aspects of data. For an array of N statistically independent release sites, each with a release probability p, the compound binomial always pertai...

journal_title：Biophysical journal

authors： Quastel DM

更新日期：1997-02-01 00:00:00

abstract：:We have established a quantum mechanics (QM)/molecular mechanics (MM) hybrid method for calculating the Raman spectra of protein-bound cofactors using the alpha-subunit of C-phycocyanin containing a phycocyanobilin (PCB) chromophore as a test case. The PCB cofactor was described with density functional theory, whereas...

journal_title：Biophysical journal

authors： Mroginski MA,Mark F,Thiel W,Hildebrandt P

更新日期：2007-09-15 00:00:00

abstract：:The (31)P-nuclear magnetic resonance chemical shift of phosphatidic acid in a membrane is sensitive to the lipid head group packing and can report qualitatively on membrane lateral compression near the aqueous interface. We have used high-resolution (31)P-nuclear magnetic resonance to evaluate the lateral compression ...

journal_title：Biophysical journal

authors： Traïkia M,Warschawski DE,Lambert O,Rigaud JL,Devaux PF

更新日期：2002-09-01 00:00:00

abstract：:Cell adhesion to extracellular matrix is mediated by receptor-ligand interactions. When a cell first contacts a surface, it spreads, exerting traction forces against the surface and forming new bonds as its contact area expands. Here, we examined the changes in shape, actin polymerization, focal adhesion formation, an...

journal_title：Biophysical journal

authors： Reinhart-King CA,Dembo M,Hammer DA

更新日期：2005-07-01 00:00:00

abstract：:Molecular interactions are often transient and might change within the window of observation, leading to changes in molecule movement. Therefore, accurate motion analysis often requires transient motion classification. Here we present an accurate and computationally efficient transient mobility analysis framework, ter...

journal_title：Biophysical journal

authors： Vega AR,Freeman SA,Grinstein S,Jaqaman K

更新日期：2018-03-13 00:00:00

abstract：:Using molecular dynamics simulations, we studied the mode of association of the cell-penetrating peptide penetratin with both a neutral and a charged bilayer. The results show that the initial peptide-lipid association is a fast process driven by electrostatic interactions. The homogeneous distribution of positively c...

journal_title：Biophysical journal

authors： Lensink MF,Christiaens B,Vandekerckhove J,Prochiantz A,Rosseneu M

更新日期：2005-02-01 00:00:00

abstract：:Mechanical properties of living cells can be determined using atomic force microscopy (AFM). In this study, a novel analysis was developed to determine the mechanical properties of adherent monolayers of pulmonary microvascular endothelial cells (ECs) using AFM and finite element modeling, which considers both the fin...

journal_title：Biophysical journal

authors： Kang I,Panneerselvam D,Panoskaltsis VP,Eppell SJ,Marchant RE,Doerschuk CM

更新日期：2008-04-15 00:00:00

abstract：:The sequence of events involved in the transition from attached liposomes to bilayer patches on hydrophilic and hydrophobic solid supports were visualized in situ by Tapping Mode atomic force microscopy in liquid. In a smooth manner, the attached liposomes spread and flattened from the outer edges toward the center un...

journal_title：Biophysical journal

authors： Jass J,Tjärnhage T,Puu G

更新日期：2000-12-01 00:00:00

abstract：:The interaction of the nonionic surfactant dodecylmaltoside (DM) with phosphatidylcholine liposomes was investigated. Permeability alterations were detected as a change in 5(6)-carboxyfluorescein released from the interior of vesicles and bilayer solubilization as a decrease in the static light scattered by liposome s...

journal_title：Biophysical journal

authors： de la Maza A,Parra JL

更新日期：1997-04-01 00:00:00

abstract：:A new model is proposed to account for the apparent conductance changes of the sodium, or early, channel in nerve fiber membranes. In this model it is assumed that the channels are gated at the interior side of the membrane and are resistively limited at the exterior side by sodium selective barriers of high resistanc...

journal_title：Biophysical journal

authors： Hoyt RC,Strieb JD

更新日期：1971-11-01 00:00:00

abstract：:Dithioation of DNA phosphate is known to enhance binding affinities, at least for some proteins. We mechanistically characterized this phenomenon for the Antennapedia homeodomain-DNA complex by integrated use of fluorescence, isothermal titration calorimetry, NMR spectroscopy, and x-ray crystallography. By fluorescenc...

journal_title：Biophysical journal

authors： Zandarashvili L,Nguyen D,Anderson KM,White MA,Gorenstein DG,Iwahara J

更新日期：2015-09-01 00:00:00

abstract：:The refractive indices, water content, and volume of wet-spun films of Na-hyaluronate have been measured as a function of relative humidity (rh). These data are used with the Lorentz-Lorenz formula to determine the optical polarizabilities of Na-hyaluronate parallel and perpendicular to the helical axis. The analysis ...

journal_title：Biophysical journal

authors： Flowers MR,Marlow RL,Lee SA,Lavalle N,Rupprecht A

更新日期：1992-08-01 00:00:00

abstract：:Despite the importance of magnetic properties of biological samples for biomagnetism and related fields, the exact magnetic susceptibilities of most biological samples in their physiological conditions are still unknown. Here we used superconducting quantum interferometer device to detect the magnetic properties of no...

journal_title：Biophysical journal

authors： Tao Q,Zhang L,Han X,Chen H,Ji X,Zhang X

更新日期：2020-02-04 00:00:00

abstract：:Allosteric regulation of protein function is recognized to be widespread throughout biology; however, knowledge of allosteric mechanisms, the molecular changes within a protein that couple one binding site to another, is limited. Although mutagenesis is often used to probe allosteric mechanisms, we consider herein wha...

journal_title：Biophysical journal

authors： Carlson GM,Fenton AW

更新日期：2016-05-10 00:00:00