Characterization of biomimetic surfaces formed from cell membranes.

abstract：:The thermophilic Bacillus caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-CspB) in 11 of the 66 residues. Stability measurements of Schmid and co-workers have implicated contributions of electrostatic interactions to the thermostability. To further elucid...

journal_title：Biophysical journal

authors： Zhou HX,Dong F

更新日期：2003-04-01 00:00:00

abstract：:The effects of lipid chain packing and permeant size and shape on permeability across lipid bilayers have been investigated in gel and liquid crystalline dipalmitoylphosphatidylcholine (DPPC) bilayers by a combined NMR line-broadening/dynamic light scattering method using seven short-chain monocarboxylic acids (formic...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1998-12-01 00:00:00

abstract：:The jaws of the bloodworm Glycera dibranchiata consist principally of protein and melanin scaffolds with small amounts of unmineralized copper (Cu) and mineralized atacamite (Cu(2)Cl(OH)(3)) fibers in distinct regions. Remarkably, when tested in air, the regions containing unmineralized Cu are the hardest, stiffest, a...

journal_title：Biophysical journal

authors： Moses DN,Pontin MG,Waite JH,Zok FW

更新日期：2008-04-15 00:00:00

abstract：:Equilibrium binding ligands usually increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. High-throughput screening for the discovery of drug-like compounds uses an assay based on thermal stabilization. The mathematical description of this stabilization is well d...

journal_title：Biophysical journal

authors： Cimmperman P,Baranauskiene L,Jachimoviciūte S,Jachno J,Torresan J,Michailoviene V,Matuliene J,Sereikaite J,Bumelis V,Matulis D

更新日期：2008-10-01 00:00:00

abstract：:Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective observation of coordinated spontaneous fluctuations in two separate detection channels that unambiguously reflect the existence o...

journal_title：Biophysical journal

authors： Heinze KG,Rarbach M,Jahnz M,Schwille P

更新日期：2002-09-01 00:00:00

abstract：:This paper is concerned with the nature of the transmembrane potential induced in an inactive nerve fiber lying in an impressed potential field within a nerve trunk. The impressed potential field is assumed to be produced by the synchronous activity of other fibers within the trunk; an expression for the induced trans...

journal_title：Biophysical journal

authors： Clark JW Jr,Plonsey R

更新日期：1971-03-01 00:00:00

abstract：:Fluorescence contributions from immobile sources present a challenge for fluorescence fluctuation spectroscopy (FFS) because the absence of signal fluctuations from stationary fluorophores leads to a biased analysis. This is especially of concern for cellular FFS studies on proteins that interact with immobile structu...

journal_title：Biophysical journal

authors： Skinner JP,Chen Y,Müller JD

更新日期：2008-03-15 00:00:00

abstract：:A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonl...

journal_title：Biophysical journal

authors： Gratton E,Limkeman M

更新日期：1983-12-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is generally used to obtain information about the number of fluorescent particles in a small volume and the diffusion coefficient from the autocorrelation function of the fluorescence signal. Here we demonstrate that photon counting histogram (PCH) analysis constitutes a nov...

journal_title：Biophysical journal

authors： Chen Y,Müller JD,So PT,Gratton E

更新日期：1999-07-01 00:00:00

abstract：:The membrane disruption mechanism of pandinin 1 (pin1), an antimicrobial peptide isolated from the venom of the African scorpion, was studied using 31P, 13C, 1H solid-state and multidimensional solution-state NMR spectroscopy. A high-resolution NMR solution structure of pin1 showed that the two distinct alpha-helical ...

journal_title：Biophysical journal

authors： Nomura K,Ferrat G,Nakajima T,Darbon H,Iwashita T,Corzo G

更新日期：2005-12-01 00:00:00

abstract：:Certain environmental parameters are accessible to cells only indirectly and require an encoding step for cells to retrieve the relevant information. A prominent example is the phenomenon of quorum sensing by microorganisms, where information about cell density is encoded by means of secreted signaling molecules. The ...

journal_title：Biophysical journal

authors： Drees B,Reiger M,Jung K,Bischofs IB

更新日期：2014-07-01 00:00:00

abstract：:The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical...

journal_title：Biophysical journal

authors： Dean KM,Roudot P,Welf ES,Danuser G,Fiolka R

更新日期：2015-06-16 00:00:00

abstract：:We explore the means by which immobilization of a substrate on a surface can increase the rate of a diffusion-controlled enzymatic reaction. A quasichemical approach is developed and compared with Brownian dynamics simulations. We use these methods to show that restricting only the orientation of the enzyme by long-ra...

journal_title：Biophysical journal

authors： Nag A,Dinner AR

更新日期：2006-02-01 00:00:00

abstract：:Biofilm infections can consist of bacterial aggregates that are an order of magnitude larger than neutrophils, phagocytic immune cells that densely surround aggregates but do not enter them. Because a neutrophil is too small to engulf the entire aggregate, it must be able to detach and engulf a few bacteria at a time ...

journal_title：Biophysical journal

authors： Davis-Fields M,Bakhtiari LA,Lan Z,Kovach KN,Wang L,Cosgriff-Hernandez EM,Gordon VD

更新日期：2019-10-15 00:00:00

abstract：:Measurements of transepithelial electrical impedance of continuously short-circuited A6 epithelia were made at audio frequencies (0.244 Hz to 10.45 kHz) to investigate the time course and extent to which prostaglandin E(2) (PGE(2)) modulates Cl(-) transport and apical membrane capacitance in this cell-cultured model e...

journal_title：Biophysical journal

authors： Păunescu TG,Helman SI

更新日期：2001-08-01 00:00:00

abstract：:We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Phosphatase exclusion generates regions of low phosphatase and high kinase activity and ...

journal_title：Biophysical journal

authors： Burroughs NJ,Lazic Z,van der Merwe PA

更新日期：2006-09-01 00:00:00

abstract：:The use of the long-lived fluorescence probe coronene (mean value of tau(FL) approximately 200 ns) is described for investigating submicrosecond lipid dynamics in DPPC model bilayer systems occurring below the lipid phase transition. Time-resolved fluorescence emission anisotropy decay profiles, measures as a function...

journal_title：Biophysical journal

authors： Davenport L,Targowski P

更新日期：1996-10-01 00:00:00

abstract：:We present higher-order moment analysis of fluorescence intensity fluctuations from individual laser scanning microscopy images applied to study monomer-oligomer distributions. We demonstrate that the number densities and brightness ratios of a mixed population of monomers and oligomers can be determined by analyzing ...

journal_title：Biophysical journal

authors： Sergeev M,Costantino S,Wiseman PW

更新日期：2006-11-15 00:00:00

abstract：:Sulfolobus solfataricus carboxypeptidase (CPSso) is a thermostable zinc-metalloenzyme with a M(r) of 43,000. Taking into account the experimentally determined zinc content of one ion per subunit, we developed two alternative 3D models, starting from the available structures of Thermoactinomyces vulgaris carboxypeptida...

journal_title：Biophysical journal

authors： Occhipinti E,Martelli PL,Spinozzi F,Corsi F,Formantici C,Molteni L,Amenitsch H,Mariani P,Tortora P,Casadio R

更新日期：2003-08-01 00:00:00

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:Here we extend the ability to predict hydrodynamic coefficients and other solution properties of rigid macromolecular structures from atomic-level structures, implemented in the computer program HYDROPRO, to models with lower, residue-level resolution. Whereas in the former case there is one bead per nonhydrogen atom,...

journal_title：Biophysical journal

authors： Ortega A,Amorós D,García de la Torre J

更新日期：2011-08-17 00:00:00

abstract：:We used optical tweezers to analyze the effect of jasplakinolide and cyclodextrin on the force exerted by lamellipodia from developing growth cones (GCs) of isolated dorsal root ganglia (DRG) neurons. We found that 25 nM of jasplakinolide, which is known to inhibit actin filament turnover, reduced both the maximal exe...

journal_title：Biophysical journal

authors： Amin L,Ercolini E,Shahapure R,Migliorini E,Torre V

更新日期：2012-06-06 00:00:00

abstract：:Immature human immunodeficiency virus (HIV) virions have a lattice of Gag and Gag-Pol proteins anchored to the lumen of their envelope. Using electron microscopy, we demonstrate that HIV virus-like particles (VLPs) assembled by the viral protein Gag and tagged at its C-terminus with the fluorescent protein Dendra2 hav...

journal_title：Biophysical journal

authors： Saha I,Saffarian S

更新日期：2020-08-04 00:00:00

abstract：:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human...

journal_title：Biophysical journal

authors： Diop M,Taylor R

更新日期：2006-05-15 00:00:00

abstract：:Two mechanisms for the interaction of alternating electrical fields with biological tissue are the development of heat, via i(2)R losses, and field-induced force effects, via differences in passive electrical properties. It has been shown that for continuous wave (CW) fields in media of physiologic electrical conducti...

journal_title：Biophysical journal

authors： Sher LD,Kresch E,Schwan HP

更新日期：1970-10-01 00:00:00

abstract：:The effects of temperature (3 degrees-26 degrees C) on the nonlinear components of the displacement current were measured in internally perfused, voltage clamped squid axons. Steps of potential were applied from a holding potential of -70mV (outside ground) to values from -130 to +70mV and either the current or its in...

journal_title：Biophysical journal

authors： Bezanilla F,Taylor RE

更新日期：1978-09-01 00:00:00

abstract：:Many traditional approaches to the analysis of sedimentation velocity data work poorly with data for low-molecular-weight solutes, which have sedimentation boundaries that are severely broadened by diffusion. An approach that has previously had some success is to directly fit these broad boundaries to approximate solu...

journal_title：Biophysical journal

authors： Philo JS

更新日期：1997-01-01 00:00:00

abstract：:The relationship between epithelial fluid transport, standing osmotic gradients, and standing hydrostatic pressure gradients has been investigated using a perturbation expansion of the governing equations. The assumptions used in the expansion are: (a) the volume of lateral intercellular space per unit volume of epith...

journal_title：Biophysical journal

authors： Mathias RT

更新日期：1985-06-01 00:00:00

abstract：:The time correlation function of the shape fluctuations of large (greater than 10 micron), cylindrical, hydrated, phospholipid-membrane vesicles consisting of one bimolecular layer was measured. The restoring force of the membrane was due to the excess curvature of a membrane element. A value for the curvature elastic...

journal_title：Biophysical journal

authors： Schneider MB,Jenkins JT,Webb WW

更新日期：1984-05-01 00:00:00

abstract：:To probe the complexity of the cell membrane organization and dynamics, it is important to obtain simple physical observables from experiments on live cells. Here we show that fluorescence correlation spectroscopy (FCS) measurements at different spatial scales enable distinguishing between different submicron confinem...

journal_title：Biophysical journal

authors： Wawrezinieck L,Rigneault H,Marguet D,Lenne PF

更新日期：2005-12-01 00:00:00