Ligand detection and discrimination by spatial relocalization: A kinase-phosphatase segregation model of TCR activation.

Abstract:

:We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Phosphatase exclusion generates regions of low phosphatase and high kinase activity and thus our model provides a framework to examine the kinetic segregation model of TCR activation. We incorporate a sequence of activation steps modeling the construction of the signalosome with a final sequestered, or high-stability, signaling state. The residence time of unbound TCRs in tyrosine kinase-rich domains is shown to be too short for accumulation of activation steps, whereas binding to an agonist lengthens the localization time and leads to generation of fully active TCRs. Agonist detection depends only on this localization, and therefore kinetic segregation represents a viable ligand detection mechanism, or signal transduction mechanism across membranes, distinct from receptor oligomerization and conformational change. We examine the degree of discrimination of agonists from a background of null (self) peptides, and from weak agonists achievable by this mechanism.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Burroughs NJ,Lazic Z,van der Merwe PA

doi

10.1529/biophysj.105.080044

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

1619-29

issue

5

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(06)71876-8

journal_volume

91

pub_type

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