Temperature effects on gating currents in the squid giant axon.

abstract：:Choice of network topology for gene regulation has been a question of interest for a long time. How do simple and more complex topologies arise? In this work, we analyze the topology of the marRAB operon in Escherichia coli, which is associated with control of expression of genes associated with conferring resistance ...

journal_title：Biophysical journal

authors： Prajapat MK,Jain K,Saini S

更新日期：2015-10-06 00:00:00

abstract：:A simulation of the function of the human heart and heart muscle has been developed in the form of a digital computer code. For a given set of values for the input variables, realistic values of the cardiac output variables are predicted. A detailed discussion of the simulation and some results obtained from its appli...

journal_title：Biophysical journal

authors： Hanna WT

更新日期：1973-07-01 00:00:00

abstract：:We have used electron paramagnetic resonance (EPR) to probe the homo- and heterooligomeric interactions of reconstituted sarcoplasmic reticulum Ca-ATPase (SERCA) and its regulator phospholamban (PLB). SERCA is responsible for restoring calcium to the sarcoplasmic reticulum to allow muscle relaxation, whereas PLB inhib...

journal_title：Biophysical journal

authors： James ZM,McCaffrey JE,Torgersen KD,Karim CB,Thomas DD

更新日期：2012-09-19 00:00:00

abstract：:Pore formation by membrane-active peptides, naturally encountered in innate immunity and infection, could have important medical and technological applications. Recently, the well-studied lytic peptide melittin has formed the basis for the development of combinatorial libraries from which potent pore-forming peptides ...

journal_title：Biophysical journal

authors： Sepehri A,PeBenito L,Pino-Angeles A,Lazaridis T

更新日期：2020-04-21 00:00:00

abstract：:The participation of amino-terminal domains in human ether-a-go-go (eag)-related gene (HERG) K(+) channel gating was studied using deleted channel variants expressed in Xenopus oocytes. Selective deletion of the HERG-specific sequence (HERG Delta138-373) located between the conserved initial amino terminus (the eag or...

journal_title：Biophysical journal

authors： Viloria CG,Barros F,Giráldez T,Gómez-Varela D,de la Peña P

更新日期：2000-07-01 00:00:00

abstract：:Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the la...

journal_title：Biophysical journal

authors： Vulin C,Di Meglio JM,Lindner AB,Daerr A,Murray A,Hersen P

更新日期：2014-05-20 00:00:00

abstract：:Diluted bull semen samples were bombarded with a 24 Mev proton beam. Dose response curves for the fraction of cells which survived the bombardment and for the average velocity of the surviving cells were measured. Target theory indicated a cross section of the sensitive volume of 2.1 x 10(-10) cm(2). Respiration measu...

journal_title：Biophysical journal

authors： Rikmenspoel R,van Herpen G

更新日期：1969-06-01 00:00:00

abstract：:The physical forces that underlie the exclusion of solutes from macromolecular surfaces can be probed in a similar way as the measurement of forces between macromolecules in condensed arrays using the osmotic stress technique and x-ray scattering. We report here the dependence of alcohol exclusion or, equivalently, th...

journal_title：Biophysical journal

authors： Stanley C,Rau DC

更新日期：2006-08-01 00:00:00

abstract：:Voltage-gated ion channels are crucial for regulation of electric activity of excitable tissues such as nerve cells, and play important roles in many diseases. During activation, the charged S4 segment in the voltage sensor domain translates across a hydrophobic core forming a barrier for the gating charges. This barr...

journal_title：Biophysical journal

authors： Schwaiger CS,Liin SI,Elinder F,Lindahl E

更新日期：2013-01-08 00:00:00

abstract：:Unlike most transmembrane proteins, phospholipids can migrate from one leaflet of the membrane to the other. Because this spontaneous lipid translocation (flip-flop) tends to be very slow, cells facilitate the process with enzymes that catalyze the transmembrane movement and thereby regulate the transbilayer lipid dis...

journal_title：Biophysical journal

authors： Doktorova M,Heberle FA,Marquardt D,Rusinova R,Sanford RL,Peyear TA,Katsaras J,Feigenson GW,Weinstein H,Andersen OS

更新日期：2019-03-05 00:00:00

abstract：:Molecular dynamics simulations supplement single-molecule pulling experiments by providing the possibility of examining the full free energy landscape using many coordinates. Here, we use an all-atom structure-based model to study the force and temperature dependence of the unfolding of the protein filamin by applying...

journal_title：Biophysical journal

authors： Sun L,Noel JK,Sulkowska JI,Levine H,Onuchic JN

更新日期：2014-12-16 00:00:00

abstract：:The distribution of a selection of cationic fluorescent dyes can be used to measure the membrane potential of individual cells with a microfluorometer. The essential attributes of these dyes include membrane permeability, low membrane binding, spectral properties which are insensitive to environment, and, of course, s...

journal_title：Biophysical journal

authors： Ehrenberg B,Montana V,Wei MD,Wuskell JP,Loew LM

更新日期：1988-05-01 00:00:00

abstract：:Two-dimensional solid-state 31P NMR has been used to investigate the orientational exchange of phospholipids in gel and liquid-crystalline aqueous multilamellar dispersions and oriented multibilayers, and in biological membranes. In liquid-crystalline L alpha multilamellar dispersions, orientational exchange originate...

journal_title：Biophysical journal

authors： Fenske DB,Jarrell HC

更新日期：1991-01-01 00:00:00

abstract：:Kinesin-1 is an ATP-driven molecular motor that transports cellular cargo along microtubules. At low loads, kinesin-1 almost always steps forward, toward microtubule plus ends, but at higher loads, it can also step backward. Backsteps are usually 8 nm but can be larger. These larger backward events of 16 nm, 24 nm, or...

journal_title：Biophysical journal

authors： Toleikis A,Carter NJ,Cross RA

更新日期：2020-11-17 00:00:00

abstract：:Cyclic 3'5' adenosine monophosphate (cAMP)-dependent-protein kinase (PKA) signaling is a fundamental regulatory pathway for mediating cellular responses to hormonal stimuli. The pathway is activated by high-affinity association of cAMP with the regulatory subunit of PKA and signal termination is achieved upon cAMP dis...

journal_title：Biophysical journal

authors： Krishnamurthy S,Moorthy BS,Xin Xiang L,Xin Shan L,Bharatham K,Tulsian NK,Mihalek I,Anand GS

更新日期：2014-09-16 00:00:00

abstract：:Flow dialysis has found widespread use in determining the dissociation constant (KD) of a protein-ligand interaction or the amount of available binding sites (E0). This method has the potency to measure both these parameters in a single experiment and in this article a method to measure simultaneously the KD and E0 is...

journal_title：Biophysical journal

authors： Veldhuis G,Vos EP,Broos J,Poolman B,Scheek RM

更新日期：2004-04-01 00:00:00

abstract：:The canonical mass balance relation derived for the central core model of the renal medulla is extended to medullary models in which an arbitrary assemblage of renal tubules and vascular capillaries exchange with each other both directly and via the medullary interstitium and in which not all of the vascular loops or ...

journal_title：Biophysical journal

authors： Stephenson JL

更新日期：1976-11-01 00:00:00

abstract：:We investigate the conditions under which nonideality in solution influences the Donnan equilibrium. Of the various parameters that characterize this equilibrium, the osmotic pressure established across the Donnan membrane is found to be particularly sensitive to intermolecular interactions between the diffusible and ...

journal_title：Biophysical journal

authors： Stell G,Joslin CG

更新日期：1986-11-01 00:00:00

abstract：:Amyloid diseases are traditionally characterized by the appearance of inter- and intracellular fibrillar protein deposits, termed amyloid. Historically, these deposits have been thought to be the etiology of the disease. However, recent evidence suggests that small oligomers of the amyloidogenic protein/peptide are th...

journal_title：Biophysical journal

authors： Ding H,Wong PT,Lee EL,Gafni A,Steel DG

更新日期：2009-08-05 00:00:00

abstract：:The genetic map of T4 (and T2) bacteriophage is circular but the DNA molecule that is liberated by phenol extraction is a linear duplex of polynucleotide chains. If the genetic map is related to the physical structure of the DNA molecule, the problem arises as to how a linear molecule can give rise to a circular map. ...

journal_title：Biophysical journal

authors： THOMAS CA Jr,RUBENSTEIN I

更新日期：1964-03-01 00:00:00

abstract：:Certain standard properties, including spontaneous curvature, are assumed for the membrane of a bilipid vesicle. Then, if there is a mechanism that causes the membrane area to increase, vesicle cleavage is found to occur. ...

journal_title：Biophysical journal

authors： Luke JC,Kaplan JI

更新日期：1979-01-01 00:00:00

abstract：:In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exh...

journal_title：Biophysical journal

authors： Linari M,Piazzesi G,Pertici I,Dantzig JA,Goldman YE,Lombardi V

更新日期：2020-03-10 00:00:00

abstract：:Dithioation of DNA phosphate is known to enhance binding affinities, at least for some proteins. We mechanistically characterized this phenomenon for the Antennapedia homeodomain-DNA complex by integrated use of fluorescence, isothermal titration calorimetry, NMR spectroscopy, and x-ray crystallography. By fluorescenc...

journal_title：Biophysical journal

authors： Zandarashvili L,Nguyen D,Anderson KM,White MA,Gorenstein DG,Iwahara J

更新日期：2015-09-01 00:00:00

abstract：:Cationic liposomes (CLs) are used worldwide as gene vectors (carriers) in nonviral clinical applications of gene delivery, albeit with unacceptably low transfection efficiencies (TE). We present three-dimensional laser scanning confocal microscopy studies revealing distinct interactions between CL-DNA complexes, for b...

journal_title：Biophysical journal

authors： Lin AJ,Slack NL,Ahmad A,George CX,Samuel CE,Safinya CR

更新日期：2003-05-01 00:00:00

abstract：:The interaction between two stiff parallel DNA molecules is discussed using linear Debye-Hückel screening theory with and without inclusion of the dielectric discontinuity at the DNA surface, taking into account the helical symmetry of DNA. The pair potential furthermore includes the amount and distribution of counter...

journal_title：Biophysical journal

authors： Harreis HM,Likos CN,Löwen H

更新日期：2003-06-01 00:00:00

abstract：:Most of the important types of interactions that occur in cells can be characterized as binding-diffusion type processes, and can be quantified by kinetic rate constants such as diffusion coefficients (D) and binding rate constants (k(on) and k(off)). Confocal FRAP is a potentially important tool for the quantitative ...

journal_title：Biophysical journal

authors： Kang M,Day CA,DiBenedetto E,Kenworthy AK

更新日期：2010-11-03 00:00:00

abstract：:Dielectrophoresis and electrorotation are commonly used to measure dielectric properties and membrane electrical parameters of biological cells. We have derived quantitative relationships for several critical points, defined in Fig. A 1, which characterize the dielectrophoretic spectrum and the electrorotational spect...

journal_title：Biophysical journal

authors： Gimsa J,Marszalek P,Loewe U,Tsong TY

更新日期：1991-10-01 00:00:00

abstract：:The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chlori...

journal_title：Biophysical journal

authors： Lakatos M,Groma GI,Ganea C,Lanyi JK,Váró G

更新日期：2002-04-01 00:00:00

abstract：:The space-clamped squid axon membrane and two versions of the Hodgkin-Huxley model (the original, and a strongly adapting version) are subjected to a first order dynamic analysis. Stable, repetitive firing is induced by phase-locking nerve impulses to sinusoidal currents. The entrained impulses are then pulse position...

journal_title：Biophysical journal

authors： Fohlmeister JF,Adelman WJ Jr,Poppele RE

更新日期：1980-04-01 00:00:00

abstract：:We introduce an approach based on the recently introduced functional mode analysis to identify collective modes of internal dynamics that maximally correlate to an external order parameter of functional interest. Input structural data can be either experimentally determined structure ensembles or simulated ensembles, ...

journal_title：Biophysical journal

authors： Krivobokova T,Briones R,Hub JS,Munk A,de Groot BL

更新日期：2012-08-22 00:00:00