Intestinal Villus Structure Contributes to Even Shedding of Epithelial Cells.

abstract：:Tet repressor (TetR) is involved in the most abundant mechanism of tetracycline (Tc) resistance of gram-negative bacteria. Raman spectra were measured for the class D TetR protein, for an oligodeoxyribonucleotide with sequence corresponding to operator site O1, and for the TetR:oligonucleotide complex. TetR forms a co...

journal_title：Biophysical journal

authors： Krafft C,Hinrichs W,Orth P,Saenger W,Welfle H

更新日期：1998-01-01 00:00:00

abstract：:Precise information regarding the transmembrane topology of mitochondrial porin is essential for understanding the mechanisms by which this protein functions. Porin acts as a channel in the outer membrane and interacts with small solutes and proteins to regulate mitochondrial function. The acquisition of high-resoluti...

journal_title：Biophysical journal

authors： Bay DC,O'Neil JD,Court DA

更新日期：2008-01-15 00:00:00

abstract：:The mechanical properties of living cells are a label-free biophysical marker of cell viability and health; however, their use has been greatly limited by low measurement throughput. Although examining individual cells at high rates is now commonplace with fluorescence activated cell sorters, development of comparable...

journal_title：Biophysical journal

authors： Sawetzki T,Eggleton CD,Desai SA,Marr DW

更新日期：2013-11-19 00:00:00

abstract：:We present here an efficient and accurate procedure for modeling of the three-dimensional structures of polypeptides in the explicit solvent water based on molecular dynamics calculations. Using the toxic domain analog of heat-stable enterotoxin as a model peptide, we examined the utilities of two molecular dynamics t...

journal_title：Biophysical journal

authors： Tsujishita H,Moriguchi I,Hirono S

更新日期：1994-06-01 00:00:00

abstract：:In this article we present a study of a new class of surfactants denoted as PEG1500-12-acyloxystearates, which have potential use as pharmaceutical solubilizers. These amphiphilic molecules present interesting properties with regard to cell damage effects. PEG1500-12-acyloxystearates with C(14) to C(16) acyloxy chains...

journal_title：Biophysical journal

authors： Vaccaro M,von Corswant C,Söderman O

更新日期：2007-12-15 00:00:00

abstract：:Theories of DNA electrophoretic separations generally treat the DNA as a free draining polymer moving in an electric field at a rate that depends on the effective charge density of the molecule. Separations can occur in sieving media ranging from ultradilute polymer solutions to tightly cross-linked gels. It has recen...

journal_title：Biophysical journal

authors： Ferree S,Blanch HW

更新日期：2004-07-01 00:00:00

abstract：:The properties of vesicle membranes prepared from 16:0-SM, 16:0-DHSM, or DPPC were characterized using steady-state and time-resolved fluorescence spectroscopy and different fluorescent reporter molecules. The acyl-chain region was probed using free and phospholipid-bound 1,6-diphenyl-1,3,5-hexatriene. 16:0-DHSM was f...

journal_title：Biophysical journal

authors： Nyholm T,Nylund M,Söderholm A,Slotte JP

更新日期：2003-02-01 00:00:00

abstract：:One of the most common swimming strategies employed by microorganisms is based on the use of rotating helical filaments, called flagella, that are powered by molecular motors. Determining the physical properties of this propulsive system is crucial to understanding the behavior of these organisms. Furthermore, the abi...

journal_title：Biophysical journal

authors： Pierce CJ,Osborne E,Mumper E,Lower BH,Lower SK,Sooryakumar R

更新日期：2019-10-01 00:00:00

abstract：:Two mesophilic/thermophilic variants of the G-domain of the elongation factor Tu were studied via molecular dynamics simulations. By analyzing the simulation data via the Voronoi space tessellation, we have found that the two proteins have the same macromolecular packing, while the water-exposed surface area is larger...

journal_title：Biophysical journal

authors： Melchionna S,Sinibaldi R,Briganti G

更新日期：2006-06-01 00:00:00

abstract：:This contribution describes measurements of lipid bilayer domain line tension based on two-dimensional thermal undulations of membranes with liquid ordered/liquid disordered phase coexistence and near-critical composition at room temperature. Lateral inhomogeneity of lipid and protein composition is currently a subjec...

journal_title：Biophysical journal

authors： Esposito C,Tian A,Melamed S,Johnson C,Tee SY,Baumgart T

更新日期：2007-11-01 00:00:00

abstract：:Chemical synaptic transmission is a fundamental component of interneuronal communications in the central nervous system (CNS). Discharge of a presynaptic vesicle containing a few thousand molecules (a quantum) of neurotransmitter into the synaptic cleft generates a transmitter concentration signal that drives postsyna...

journal_title：Biophysical journal

authors： Uteshev VV,Pennefather PS

更新日期：1997-03-01 00:00:00

abstract：:Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing, and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent (near) field, and it is a popular contrast generator for surface-selective fluoresc...

journal_title：Biophysical journal

authors： Oheim M,Salomon A,Brunstein M

更新日期：2020-05-19 00:00:00

abstract：:The unfolding and folding of protein barnase has been extensively investigated in bulk conditions under the effect of denaturant and temperature. These experiments provided information about structural and kinetic features of both the native and the unfolded states of the protein, and debates about the possible existe...

journal_title：Biophysical journal

authors： Alemany A,Rey-Serra B,Frutos S,Cecconi C,Ritort F

更新日期：2016-01-05 00:00:00

abstract：:The rat connexin40 gap junction channel is permeable to monovalent cations including tetramethylammonium and tetraethylammonium ions. Larger tetraalkyammonium (TAA(+)) ions beginning with tetrabutylammonium (TBA(+)) reduced KCl junctional currents disproportionately. Ionic blockade by tetrapentylammonium (TPeA(+)) and...

journal_title：Biophysical journal

authors： Musa H,Gough JD,Lees WJ,Veenstra RD

更新日期：2001-12-01 00:00:00

abstract：:Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the la...

journal_title：Biophysical journal

authors： Vulin C,Di Meglio JM,Lindner AB,Daerr A,Murray A,Hersen P

更新日期：2014-05-20 00:00:00

abstract：:Epigenetic modifications can extend over long genomic regions to form domain-level chromatin states that play critical roles in gene regulation. The molecular mechanism for the establishment and maintenance of these states is not fully understood and remains challenging to study with existing experimental techniques. ...

journal_title：Biophysical journal

authors： Xie WJ,Zhang B

更新日期：2019-05-21 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:Although cell-cell interactions are known to significantly affect the kinetics of intracellular ice formation (IIF) during tissue freezing, this effect is not well understood. Progress in elucidating the mechanism and role of intercellular ice propagation in tissue freezing has been hampered in part by limitations in ...

journal_title：Biophysical journal

authors： Irimia D,Karlsson JO

更新日期：2005-01-01 00:00:00

abstract：:In the context of reduced protein models, Monte Carlo simulations of three de novo designed helical proteins (four-member helical bundle) were performed. At low temperatures, for all proteins under consideration, protein-like folds having different topologies were obtained from random starting conformations. These sim...

journal_title：Biophysical journal

authors： Sikorski A,Kolinski A,Skolnick J

更新日期：1998-07-01 00:00:00

abstract：:A new microscopic technique is demonstrated that combines attributes from both near-field scanning optical microscopy (NSOM) and fluorescence resonance energy transfer (FRET). The method relies on attaching the acceptor dye of a FRET pair to the end of a near-field fiber optic probe. Light exiting the NSOM probe, whic...

journal_title：Biophysical journal

authors： Vickery SA,Dunn RC

更新日期：1999-04-01 00:00:00

abstract：:Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress condition...

journal_title：Biophysical journal

authors： Leekumjorn S,Wu Y,Sum AK,Chan C

更新日期：2008-04-01 00:00:00

abstract：:Mathematical models are increasingly important in biology, and testability is becoming a critical issue. One limitation is that one model simulation tests a parameter set representing one instance of the biological counterpart, whereas biological systems are heterogeneous in their properties and behavior, and a model ...

journal_title：Biophysical journal

authors： Tomaiuolo M,Bertram R,Leng G,Tabak J

更新日期：2012-11-07 00:00:00

abstract：:The electric quadrupole moment of the deuterium nucleus provides a nuclear magnetic resonance (NMR) probe of electric field gradients, and thereby of organization of tissue water. 8-17% of H(2)O in rat muscle and brain was replaced by D(2)O from 50% deuterated drinking water. The peak height of the steady-state NMR sp...

journal_title：Biophysical journal

authors： Cope FW

更新日期：1969-03-01 00:00:00

abstract：:Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one c...

journal_title：Biophysical journal

authors： Merkel R,Kirchgessner N,Cesa CM,Hoffmann B

更新日期：2007-11-01 00:00:00

abstract：:The accurate prediction of membrane-insertion probability for arbitrary protein sequences is a critical challenge to identifying membrane proteins and determining their folded structures. Although algorithms based on sequence statistics have had moderate success, a complete understanding of the energetic factors that ...

journal_title：Biophysical journal

authors： Gumbart J,Roux B

更新日期：2012-02-22 00:00:00

abstract：:Glasses used to fabricate patch pipettes may release components which affect ion channels (Cota, G., and C.M. Armstrong. 1988. Biophys. J. 53:107-109; Furman, R.E., and J.C. Tanaka. 1988. Biophys. J. 53:287-292; Rojas, L., and C. Zuazaga. 1988. Neurosci. Lett. 88:39-44). The gating properties of maxi K+ channels from ...

journal_title：Biophysical journal

authors： Copello J,Simon B,Segal Y,Wehner F,Ramanujam VM,Alcock N,Reuss L

更新日期：1991-10-01 00:00:00

abstract：:Many toxins and antimicrobial peptides permeabilize membrane vesicles by forming multimeric pores. Determination of the size of such pores is an important first step for understanding their structure and the mechanism of their self-assembly. We report a simple method for sizing pores in vesicles based on the different...

journal_title：Biophysical journal

authors： Ladokhin AS,Selsted ME,White SH

更新日期：1997-04-01 00:00:00

abstract：:The rotation of the lever arm of myosin cross-bridges is believed to be responsible for muscle contraction. To resolve details of this rotation, it is necessary to observe a single cross-bridge. It is still impossible to do so in muscle fiber, but it is possible to investigate a small population of cross-bridges by si...

journal_title：Biophysical journal

authors： Borejdo J,Shepard A,Akopova I,Grudzinski W,Malicka J

更新日期：2004-12-01 00:00:00

abstract：:Gene regulation involves a hierarchy of events that extend from specific protein-DNA interactions to the combinatorial assembly of nucleoprotein complexes. The effects of DNA sequence on these processes have typically been studied based either on its quantitative connection with single-domain binding free energies or ...

journal_title：Biophysical journal

authors： Vilar JM

更新日期：2010-10-20 00:00:00

abstract：:The stability of the structure of double-stranded DNA in the salt-free solution is discussed on the basis of the polyelectrolyte theory. Assuming that DNA is an infinitely long rod, and the formation of double strands is divided into combining process and folding process, the free energy changes required in these proc...

journal_title：Biophysical journal

authors： OHNISHI T

更新日期：1963-11-01 00:00:00