Scanning near-field fluorescence resonance energy transfer microscopy.

abstract：:In this article we present a study of a new class of surfactants denoted as PEG1500-12-acyloxystearates, which have potential use as pharmaceutical solubilizers. These amphiphilic molecules present interesting properties with regard to cell damage effects. PEG1500-12-acyloxystearates with C(14) to C(16) acyloxy chains...

journal_title：Biophysical journal

authors： Vaccaro M,von Corswant C,Söderman O

更新日期：2007-12-15 00:00:00

abstract：:Most of the important types of interactions that occur in cells can be characterized as binding-diffusion type processes, and can be quantified by kinetic rate constants such as diffusion coefficients (D) and binding rate constants (k(on) and k(off)). Confocal FRAP is a potentially important tool for the quantitative ...

journal_title：Biophysical journal

authors： Kang M,Day CA,DiBenedetto E,Kenworthy AK

更新日期：2010-11-03 00:00:00

abstract：:Rearrangement of the actin cytoskeleton is integral to cell shape and function. Actin-binding proteins, e.g., filamin, can naturally contribute to the mechanics and function of the actin cytoskeleton. The molecular mechanical bases for filamin's function in actin cytoskeletal reorganization are examined here using mol...

journal_title：Biophysical journal

authors： Kolahi KS,Mofrad MR

更新日期：2008-02-01 00:00:00

abstract：:Cholesterol, due to its condensing effect, is considered an important regulator of membrane thickness. Other sterols, due to their structural similarities to cholesterol, are often assumed to have a universal effect on membrane properties similar to the condensing effect of cholesterol, albeit possibly to different de...

journal_title：Biophysical journal

authors： Hung WC,Lee MT,Chung H,Sun YT,Chen H,Charron NE,Huang HW

更新日期：2016-05-10 00:00:00

abstract：:The force-extension curve of single myosin subfragment-1 molecules, interacting in the rigor state with an actin filament, has been investigated at low [ATP] by applying a slow triangle-wave movement to the optical traps holding a bead-actin-bead dumbbell. In combination with a measurement of the overall stiffness of ...

journal_title：Biophysical journal

authors： Lewalle A,Steffen W,Stevenson O,Ouyang Z,Sleep J

更新日期：2008-03-15 00:00:00

abstract：:Glucose-embedded bacteriorhodopsin shows M-intermediates with different Amide I infrared bands when samples are illuminated at 240 or 260 K, in contrast with fully hydrated samples where a single M-intermediate is formed at all temperatures. In hydrated, but not in glucose-embedded specimens, the N intermediate is for...

journal_title：Biophysical journal

authors： Vonck J,Han BG,Burkard F,Perkins GA,Glaeser RM

更新日期：1994-09-01 00:00:00

abstract：:Many traditional approaches to the analysis of sedimentation velocity data work poorly with data for low-molecular-weight solutes, which have sedimentation boundaries that are severely broadened by diffusion. An approach that has previously had some success is to directly fit these broad boundaries to approximate solu...

journal_title：Biophysical journal

authors： Philo JS

更新日期：1997-01-01 00:00:00

abstract：:The structure of crotapotin, a protein extracted, from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration Rg = 13.6 A, volume v = 16.2 x 10(3) A3 A3 and maximal dimension Dmax = 46 A...

journal_title：Biophysical journal

authors： Abrego JR,Craievich AF,Mascarenhas YP,Laure CJ

更新日期：1993-02-01 00:00:00

abstract：:The hyperpolarization-activated cyclic nucleotide-modulated (HCN) cation channels are opened by membrane hyperpolarization, while their activation is modulated by the binding of cyclic adenosine monophosphate (cAMP) in the cytoplasm. Here we investigate the molecular basis of cAMP channel modulation by performing mole...

journal_title：Biophysical journal

authors： Berrera M,Pantano S,Carloni P

更新日期：2006-05-15 00:00:00

abstract：:The closed-state crystal structure of prokaryotic inward rectifier, KirBac1.1, has implicated four inner helical phenylalanines near the cytoplasmic side as a possible locus of the channel gate. In the present study, we investigate whether this structural feature corresponds to the physiological pH gate of the renal i...

journal_title：Biophysical journal

authors： Sackin H,Nanazashvili M,Palmer LG,Krambis M,Walters DE

更新日期：2005-04-01 00:00:00

abstract：:Single epithelial Na+ channels incorporated into planar lipid bilayers were studied to determine the effects of Na concentration on its own conductance. Amiloride-sensitive Na+ channels were obtained from apical membrane vesicles made from A6 cells, a continuous epithelial cell-line derived from amphibian kidney. Sing...

journal_title：Biophysical journal

authors： Olans L,Sariban-Sohraby S,Benos DJ

更新日期：1984-12-01 00:00:00

abstract：:The bovine eye lens protein, alpha L crystallin, has been studied with photon correlation spectroscopy and statical light scattering in the concentration range up to 200 g/l in different solvent conditions. At higher concentration (c greater than 70 g/l) the scattering behavior is quite complicated, which results in n...

journal_title：Biophysical journal

authors： Andries C,Clauwaert J

更新日期：1985-05-01 00:00:00

abstract：:Diluted bull semen samples were bombarded with a 24 Mev proton beam. Dose response curves for the fraction of cells which survived the bombardment and for the average velocity of the surviving cells were measured. Target theory indicated a cross section of the sensitive volume of 2.1 x 10(-10) cm(2). Respiration measu...

journal_title：Biophysical journal

authors： Rikmenspoel R,van Herpen G

更新日期：1969-06-01 00:00:00

abstract：:The dynamics of proton transfer between the surface of purple membrane and the aqueous bulk have recently been investigated by the Laser Induced Proton Pulse Method. Following a Delta-function release of protons to the bulk, the system was seen to regain its state of equilibrium within a few hundreds of microseconds. ...

journal_title：Biophysical journal

authors： Schätzler B,Dencher NA,Tittor J,Oesterhelt D,Yaniv-Checover S,Nachliel E,Gutman M

更新日期：2003-01-01 00:00:00

abstract：:In this work we tested the hypothesis that transgenic sustained overexpression of IGF-1 prevents age-dependent decreases in charge movement and intracellular Ca(2+) in skeletal muscle fibers. To this end, short flexor digitorum brevis (FDB) muscle fibers from 5-7- and 21-24-month-old FVB (wild-type) and S1S2 (IGF-1 tr...

journal_title：Biophysical journal

authors： Wang ZM,Messi ML,Delbono O

更新日期：2002-03-01 00:00:00

abstract：:Raman spectroscopic data indicate that the conformations of the two hydrocarbon chains of dipalmitoyl phosphatidylcholine in aqueous dispersions of the lipid differ signficantly. The compounds 1-palmitoyl, 2-palmitoyl-d31-3-sn-phosphatidylcholine and 1-palmitoyl-d31, 2-palmitoyl-3-sn-phosphatidylcholine were synthesiz...

journal_title：Biophysical journal

authors： Gaber BP,Yager P,Peticolas WL

更新日期：1978-12-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:Guanidinium and acetamidinium, when added to the bathing solution in concentrations of approximately 0.1M, cause brief blocks in the single channel potassium currents from channels formed in planar lipid bilayers by gramicidin A. Single channel lifetimes are not affected indicating that the channel structure is not mo...

journal_title：Biophysical journal

authors： Hemsley G,Busath D

更新日期：1991-04-01 00:00:00

abstract：:Pore formation by membrane-active peptides, naturally encountered in innate immunity and infection, could have important medical and technological applications. Recently, the well-studied lytic peptide melittin has formed the basis for the development of combinatorial libraries from which potent pore-forming peptides ...

journal_title：Biophysical journal

authors： Sepehri A,PeBenito L,Pino-Angeles A,Lazaridis T

更新日期：2020-04-21 00:00:00

abstract：:The motility of Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion...

journal_title：Biophysical journal

authors： Rogers SS,Waigh TA,Lu JR

更新日期：2008-04-15 00:00:00

abstract：:The current article describes a new two-dimensional lambda-dynamics method to include proton tautomerism in continuous constant pH molecular dynamics (CPHMD) simulations. The two-dimensional lambda-dynamics framework is used to devise a tautomeric state titration model for the CPHMD simulations involving carboxyl and ...

journal_title：Biophysical journal

authors： Khandogin J,Brooks CL 3rd

更新日期：2005-07-01 00:00:00

abstract：:N-BAR domains are protein modules that bind to and induce curvature in membranes via a charged concave surface and N-terminal amphipathic helices. Recently, molecular dynamics simulations have demonstrated that the N-BAR domain can induce a strong local curvature that matches the curvature of the BAR domain surface fa...

journal_title：Biophysical journal

authors： Blood PD,Swenson RD,Voth GA

更新日期：2008-08-01 00:00:00

abstract：:The effects of the Ca(2+)-induced Ca2+ release blocker procaine on individual sarcoplasmic reticulum Ca2+ release channels have been examined in planar lipid bilayers. Procaine did not reduce the single channel conductance nor appreciably shorten the mean open times of the channel; rather, it increased the longest clo...

journal_title：Biophysical journal

authors： Zahradníková A,Palade P

更新日期：1993-04-01 00:00:00

abstract：:Biochemical systems lose their homogeneity at a mesoscopic scale; physical parameters vary sharply over a scale of a few nanometers.In this manuscript, we demonstrate how proton diffusion studies can report the microscopic properties of inhomogeneous systems.The method used for this purpose was the laser induced proto...

journal_title：Biophysical journal

authors： Gutman M,Nachliel E,Kiryati S

更新日期：1992-07-01 00:00:00

abstract：:Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitu...

journal_title：Biophysical journal

authors： Murovec T,Sweeney DC,Latouche E,Davalos RV,Brosseau C

更新日期：2016-11-15 00:00:00

abstract：:The determination of intracellular protein concentrations is a prerequisite for understanding protein interaction networks in systems biology. Today, protein quantification is based either on mass spectrometry, which requires large cell numbers and sophisticated measurement protocols, or on quantitative Western blotti...

journal_title：Biophysical journal

authors： Chung YD,Sinzinger MD,Bovee-Geurts P,Krause M,Dinkla S,Joosten I,Koopman WJ,Adjobo-Hermans MJ,Brock R

更新日期：2011-12-07 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation with fluorescence detection has emerged as a powerful method for the study of interacting systems of macromolecules. It combines picomolar sensitivity with high hydrodynamic resolution, and can be carried out with photoswitchable fluorophores for multicomponent dis...

journal_title：Biophysical journal

authors： Chaturvedi SK,Zhao H,Schuck P

更新日期：2017-04-11 00:00:00

abstract：:Stepwise two-photon excitation of chlorophyll a and b in the higher plant main light-harvesting complex (LHC II) and the minor complex CP29 (as well as in organic solution) with 100-fs pulses in the Q(y) region results in a weak blue fluorescence. The dependence of the spectral shape of the blue fluorescence on excita...

journal_title：Biophysical journal

authors： Leupold D,Teuchner K,Ehlert J,Irrgang KD,Renger G,Lokstein H

更新日期：2002-03-01 00:00:00

abstract：:The chemical shifts of specific (13)C and (15)N labels distributed throughout KIAGKIA-KIAGKIA-KIAGKIA (K3), an amphiphilic 21-residue antimicrobial peptide, prove that the peptide is in an all alpha-helical conformation in the bilayers of multilamellar vesicles (MLVs) containing dipalmitoylphosphatidylcholine and dipa...

journal_title：Biophysical journal

authors： Toke O,Maloy WL,Kim SJ,Blazyk J,Schaefer J

更新日期：2004-07-01 00:00:00