Properties of palmitoyl phosphatidylcholine, sphingomyelin, and dihydrosphingomyelin bilayer membranes as reported by different fluorescent reporter molecules.

abstract：:The structural changes in the photoreaction cycle of bacteriorhodopsin, a light-driven proton pump, was investigated at a resolution of 7 angstroms by a time-resolved x-ray diffraction experiment utilizing synchrotron x rays from an undulator of SPring-8. The x-ray diffraction measurement system, used in coupling with...

journal_title：Biophysical journal

authors： Oka T,Inoue K,Kataoka M,Yagi N

更新日期：2005-01-01 00:00:00

abstract：:The change in capacity of squid axon membrane during hyper- and depolarizations was investigated in the absence of ionic currents after the membrane was treated with pronase. In the presence of the inactivation process (h parameter), failure to observe the gating current in the frequency domain was attributed to the r...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1978-04-01 00:00:00

abstract：:4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that have terminal -C(NH2)2+ groups. The hydration changes that accompany their binding to the minor groove of the (AATT)2 sequence have been studied using the osmotic stress technique with fluorescence spectroscopy. The affinity...

journal_title：Biophysical journal

authors： Degtyareva NN,Wallace BD,Bryant AR,Loo KM,Petty JT

更新日期：2007-02-01 00:00:00

abstract：:We analyze a model based on receptor modification for the cAMP signaling system that controls aggregation of the slime mold Dictyostelium discoideum after starvation. The model takes into account both the desensitization of the cAMP receptor by reversible phosphorylation and the activation of adenylate cyclase that fo...

journal_title：Biophysical journal

authors： Martiel JL,Goldbeter A

更新日期：1987-11-01 00:00:00

abstract：:Using both analytical solutions obtained from simplified systems and numerical results from more realistic cases, we investigate the role played by the dielectric constant of membrane proteins epsilon(p) and pore water epsilon(w) in permeation of ions across channels. We show that the boundary and its curvature are th...

journal_title：Biophysical journal

authors： Baştuğ T,Kuyucak S

更新日期：2003-05-01 00:00:00

abstract：:Here we model the Alzheimer beta-peptide ion channel with the goal of obtaining insight into the mechanism of amyloid toxicity. The models are built based on NMR data of the oligomers, with the universal U-shaped (strand-turn-strand) motif. After 30-ns simulations in the bilayer, the channel dimensions, shapes and sub...

journal_title：Biophysical journal

authors： Jang H,Zheng J,Nussinov R

更新日期：2007-09-15 00:00:00

abstract：:A sample preparation method using spherical glass ampoules has been used to achieve 1.5-Hz resolution in 1H magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of aqueous multilamellar dispersions of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (P...

journal_title：Biophysical journal

authors： Zhou Z,Sayer BG,Hughes DW,Stark RE,Epand RM

更新日期：1999-01-01 00:00:00

abstract：:Theoretical models predict that macromolecular crowding can increase protein folding stability, but depending on details of the models (e.g., how the denatured state is represented), the level of stabilization predicted can be very different. In this study, we represented the native and denatured states atomistically,...

journal_title：Biophysical journal

authors： Qin S,Zhou HX

更新日期：2009-07-08 00:00:00

abstract：:Membrane potential of aortic endothelial cells under resting conditions is dominated by inward-rectifier K(+) channels belonging to the Kir 2 family. Regulation of endothelial Kir by membrane cholesterol was studied in bovine aortic endothelial cells by altering the sterol composition of the cell membrane. Our results...

journal_title：Biophysical journal

authors： Romanenko VG,Rothblat GH,Levitan I

更新日期：2002-12-01 00:00:00

abstract：:The Ca(2+)-permeable cation channel TRPA1 acts as an ionotropic receptor for various pungent compounds and as a noxious cold sensor in sensory neurons. It is unclear what proportion of the TRPA1-mediated current is carried by Ca(2+) ions and how the permeation pathway changes during stimulation. Here, based on the rel...

journal_title：Biophysical journal

authors： Karashima Y,Prenen J,Talavera K,Janssens A,Voets T,Nilius B

更新日期：2010-03-03 00:00:00

abstract：:Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

journal_title：Biophysical journal

authors： Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

更新日期：2008-06-01 00:00:00

abstract：:We have investigated the phase behavior of self-assembled lyotropic liquid crystals (LC) formed by ternary mixtures of oleoylethanolamide (OEA), water and arginine. OEA, a natural analog of the endogenous cannabinoid anandamide involved in the peripheral regulation of feeding, was selected as a main component due to i...

journal_title：Biophysical journal

authors： Mohammady SZ,Pouzot M,Mezzenga R

更新日期：2009-02-18 00:00:00

abstract：:The microsecond folding and unfolding kinetics of ovine prion proteins (ovPrP) were measured under various solution conditions. A fragment comprising residues 94-233 of the full-length ovPrP was studied for four variants with differing susceptibilities to classical scrapie in sheep. The observed biexponential unfoldin...

journal_title：Biophysical journal

authors： Chen KC,Xu M,Wedemeyer WJ,Roder H

更新日期：2011-09-07 00:00:00

abstract：:Time-resolved dielectric loss (TRDL) measurements are reported for the photochemical excitation of bacteriorhodopsin (bR) in solid films of Halobacterium halobium purple membranes. These measurements provide an independent confirmation for the existence of an important component of charge separation in these membranes...

journal_title：Biophysical journal

authors： McIntosh AR,Boucher F

更新日期：1991-07-01 00:00:00

abstract：:Experimental variables of optical tweezers instrumentation that affect RNA folding/unfolding kinetics were investigated. A model RNA hairpin, P5ab, was attached to two micron-sized beads through hybrid RNA/DNA handles; one bead was trapped by dual-beam lasers and the other was held by a micropipette. Several experimen...

journal_title：Biophysical journal

authors： Wen JD,Manosas M,Li PT,Smith SB,Bustamante C,Ritort F,Tinoco I Jr

更新日期：2007-05-01 00:00:00

abstract：:To understand the thermodynamic exclusion of Na(+) relative to K(+) from the S(2) site of the selectivity filter, the distribution P(X)(epsilon) (X = K(+) or Na(+)) of the binding energy (epsilon) of the ion with the channel is analyzed using the potential distribution theorem. By expressing the excess chemical potent...

journal_title：Biophysical journal

authors： Dixit PD,Merchant S,Asthagiri D

更新日期：2009-03-18 00:00:00

abstract：:A new electrophoresis model of charged components in a spherical phospholipid vesicle is proposed. In the new model the effective local tangential electric field is a result of the uniform external electric field modified by the electric field of redistributed charges. The modification is calculated on the basis of th...

journal_title：Biophysical journal

authors： Piasecki W,Salwiński L,Froncisz W

更新日期：1997-02-01 00:00:00

abstract：:The structure of the Bowman-Birk inhibitor from Vigna unguiculata seeds (BTCI) in complex with beta-trypsin was solved and refined at 1.55 A to a crystallographic R(factor) of 0.154 and R(free) of 0.169, and represents the highest resolution for a Bowman-Birk inhibitor structure to date. The BTCI-trypsin interface is ...

journal_title：Biophysical journal

authors： Barbosa JA,Silva LP,Teles RC,Esteves GF,Azevedo RB,Ventura MM,de Freitas SM

更新日期：2007-03-01 00:00:00

abstract：:Verapamil and amlodipine are calcium ion influx inhibitors of wide clinical use. They are partially charged at neutral pH and exhibit amphiphilic properties. The noncharged species can easily cross the lipid membrane. We have measured with solid-state NMR the structural changes induced by verapamil upon incorporation ...

journal_title：Biophysical journal

authors： Meier M,Blatter XL,Seelig A,Seelig J

更新日期：2006-10-15 00:00:00

abstract：:Voltage-dependent G-protein inhibition of N-type calcium channels reduces presynaptic calcium entry, sharply attenuating neurotransmitter release. Studies in neurons demonstrate that G-proteins have multiple modulatory effects on N-type channels. The observed changes may reflect genuine complexity in G-protein action ...

journal_title：Biophysical journal

authors： Patil PG,de Leon M,Reed RR,Dubel S,Snutch TP,Yue DT

更新日期：1996-11-01 00:00:00

abstract：:To investigate the force generation properties of Chlamydomonas axonemal inner-arm dyneins in response to external force, we analyzed microtubule gliding on dynein-coated surfaces under shear flow. When inner-arm dynein c was used, microtubule translocation in the downstream direction accelerated with increasing flow ...

journal_title：Biophysical journal

authors： Kikushima K,Kamiya R

更新日期：2009-09-16 00:00:00

abstract：:Articular cartilage is the connective tissue that lines joints and provides a smooth surface for joint motion. Because cartilage is avascular, molecular transport occurs primarily via diffusion or convection, and cartilage matrix structure and composition may affect diffusive transport. Because of the inhomogeneous co...

journal_title：Biophysical journal

authors： Leddy HA,Guilak F

更新日期：2008-11-15 00:00:00

abstract：:In diseases of the brain, the distribution and properties of ion channels display deviations from healthy control subjects. We studied three cases of ion channel alteration related to epileptogenesis. The first case of ion channel alteration represents an enhanced sodium current, the second case addresses the downregu...

journal_title：Biophysical journal

authors： Tigerholm J,Fransén E

更新日期：2011-10-19 00:00:00

abstract：:We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These chang...

journal_title：Biophysical journal

authors： Miake-Lye RC,Doniach S,Hodgson KO

更新日期：1983-03-01 00:00:00

abstract：:Two monocysteine mutants of cardiac muscle troponin C, cTnC(C35S) and cTnC(C84S), were genetically generated and labeled with the fluorescent probe 2-[4'-(iodoacetamido)anilino]naphthalene-6-sulfonic acid (IAANS) at Cys-84 and Cys-35, respectively. Cys-84 is located on helix D in the regulatory N-domain, and Cys-35 is...

journal_title：Biophysical journal

authors： Dong WJ,Wang CK,Gordon AM,Cheung HC

更新日期：1997-02-01 00:00:00

abstract：:Equations are derived describing the dispersion of a permeable solute during Poiseuille flow in a capillary model. It is shown that for the normal range of physiological parameters such as capillary radius, capillary length, blood flow, permeability coefficients, and diffusion constants, the center of mass of a bolus ...

journal_title：Biophysical journal

authors： Pollock F,Blum JJ

更新日期：1966-01-01 00:00:00

abstract：:The piezoelectric properties of single collagen type I fibrils in fascia were imaged with sub-20 nm spatial resolution using piezoresponse force microscopy. A detailed analysis of the piezoresponse force microscopy signal in controlled tip-fibril geometry revealed shear piezoelectricity parallel to the fibril axis. Th...

journal_title：Biophysical journal

authors： Harnagea C,Vallières M,Pfeffer CP,Wu D,Olsen BR,Pignolet A,Légaré F,Gruverman A

更新日期：2010-06-16 00:00:00

abstract：:Electrorotation of fixed red blood cells has been investigated in the frequency range between 16 Hz and 30 MHz. The rotation was studied as a function of electrolyte conductivity and surface charge density. Between 16 Hz and 1 kHz, fixed red blood cells undergo cofield rotation. The maximum of cofield rotation occurs ...

journal_title：Biophysical journal

authors： Georgieva R,Neu B,Shilov VM,Knippel E,Budde A,Latza R,Donath E,Kiesewetter H,Bäumler H

更新日期：1998-04-01 00:00:00

abstract：:For many cell types, growth, differentiation, and motility are dependent on receptor-mediated adhesion to ligand-coated surfaces. Focal contacts are strong, specialized, adhesive connections between cell and substrate in which receptors aggregate and connect extracellular ligand to intracellular cytoskeletal molecules...

journal_title：Biophysical journal

authors： Ward MD,Hammer DA

更新日期：1993-03-01 00:00:00

abstract：:A computational framework is presented for studying the mechanical response of macromolecules. The method combines a continuum mechanics (CM) model for the mechanical properties of the macromolecule with a continuum electrostatic (CE) treatment of solvation. The molecules are represented by their shape and key physico...

journal_title：Biophysical journal

authors： Ma L,Yethiraj A,Chen X,Cui Q

更新日期：2009-05-06 00:00:00