Abstract:
:Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm).
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Merkel R,Kirchgessner N,Cesa CM,Hoffmann Bdoi
10.1529/biophysj.107.111328subject
Has Abstractpub_date
2007-11-01 00:00:00pages
3314-23issue
9eissn
0006-3495issn
1542-0086pii
S0006-3495(07)71585-0journal_volume
93pub_type
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