Reaction fields in the environment of fluorescent probes: polarity profiles in membranes.

abstract：:The dynamics of proton transfer between the surface of purple membrane and the aqueous bulk have recently been investigated by the Laser Induced Proton Pulse Method. Following a Delta-function release of protons to the bulk, the system was seen to regain its state of equilibrium within a few hundreds of microseconds. ...

journal_title：Biophysical journal

authors： Schätzler B,Dencher NA,Tittor J,Oesterhelt D,Yaniv-Checover S,Nachliel E,Gutman M

更新日期：2003-01-01 00:00:00

abstract：:Two different spin labels, N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)iodoacetamide (IPSL) and (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate (MTSSL), and two different fluorescent labels 5-((((2-iodoacetyl)amino)-ethyl)amino)naphtalene-1-sulfonic acid (IAEDANS) and 6-bromoacetyl-2-dimetylaminon...

journal_title：Biophysical journal

authors： Hammarström P,Owenius R,Mårtensson LG,Carlsson U,Lindgren M

更新日期：2001-06-01 00:00:00

abstract：:The force-extension curve of single myosin subfragment-1 molecules, interacting in the rigor state with an actin filament, has been investigated at low [ATP] by applying a slow triangle-wave movement to the optical traps holding a bead-actin-bead dumbbell. In combination with a measurement of the overall stiffness of ...

journal_title：Biophysical journal

authors： Lewalle A,Steffen W,Stevenson O,Ouyang Z,Sleep J

更新日期：2008-03-15 00:00:00

abstract：:The functional organization of prokaryotic cell membranes, which is essential for many cellular processes, has been challenging to analyze due to the small size and nonflat geometry of bacterial cells. Here, we use single-molecule fluorescence microscopy and three-dimensional quantitative analyses in live Escherichia ...

journal_title：Biophysical journal

authors： Oswald F,Varadarajan A,Lill H,Peterman EJ,Bollen YJ

更新日期：2016-03-08 00:00:00

abstract：:We have obtained clear evidence for the flexibility of native scallop adductor thin filaments by studying the temperature and ionic strength dependence of the average decay constants obtained from intensity fluctuation spectroscopic (IFS) measurements. The low-angle (10-25 degrees ), average decay constants obtained f...

journal_title：Biophysical journal

authors： Newman J,Carlson FD

更新日期：1980-01-01 00:00:00

abstract：:Understanding exposure thresholds for the response of biological systems to extremely low frequency (ELF) electric and magnetic fields is a fundamental problem of long-standing interest. We consider a two-state model for voltage-gated channels in the membrane of an isolated elongated cell (Lcell = 1 mm; rcell = 25 mic...

journal_title：Biophysical journal

authors： Weaver JC,Vaughan TE,Adair RK,Astumian RD

更新日期：1998-11-01 00:00:00

abstract：:Some low molecular weight biomolecules, i.e., NAD(P)H, are unstable at high temperatures. The use of these biomolecules by thermophilic microorganisms has been scarcely analyzed. Herein, NADH stability has been studied at different temperatures and viscosities. NADH decay increased at increasing temperatures. At incre...

journal_title：Biophysical journal

authors： Cuecas A,Cruces J,Galisteo-López JF,Peng X,Gonzalez JM

更新日期：2016-08-23 00:00:00

abstract：:Probes and biosensors that incorporate luminescent Tb(III) or Eu(III) complexes are promising for cellular imaging because time-gated microscopes can detect their long-lifetime (approximately milliseconds) emission without interference from short-lifetime (approximately nanoseconds) fluorescence background. Moreover, ...

journal_title：Biophysical journal

authors： Rajendran M,Miller LW

更新日期：2015-07-21 00:00:00

abstract：:We study the folding mechanism of a triple beta-strand WW domain from the Formin binding protein 28 (FBP28) at atomic resolution with explicit water model using replica exchange molecular dynamics computer simulations. Extended sampling over a wide range of temperatures to obtain the free energy, enthalpy, and entropy...

journal_title：Biophysical journal

authors： Mu Y,Nordenskiöld L,Tam JP

更新日期：2006-06-01 00:00:00

abstract：:Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was develope...

journal_title：Biophysical journal

authors： Sengupta K,Aranda-Espinoza H,Smith L,Janmey P,Hammer D

更新日期：2006-12-15 00:00:00

abstract：:Pressure versus distance relations have been obtained for solid (gel) and neat (liquid-crystalline) phase uncharged lipid bilayers by the use of x-ray diffraction analysis of osmotically stressed monoglyceride aqueous dispersions and multilayers. For solid phase monoelaidin bilayers, the interbilayer repulsive pressur...

journal_title：Biophysical journal

authors： McIntosh TJ,Magid AD,Simon SA

更新日期：1989-05-01 00:00:00

abstract：:The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that FR-...

journal_title：Biophysical journal

authors： Chen H,Yang J,Low PS,Cheng JX

更新日期：2008-02-15 00:00:00

abstract：:Verapamil and amlodipine are calcium ion influx inhibitors of wide clinical use. They are partially charged at neutral pH and exhibit amphiphilic properties. The noncharged species can easily cross the lipid membrane. We have measured with solid-state NMR the structural changes induced by verapamil upon incorporation ...

journal_title：Biophysical journal

authors： Meier M,Blatter XL,Seelig A,Seelig J

更新日期：2006-10-15 00:00:00

abstract：:Cell motion is driven by interplay between the actin cytoskeleton and the cell adhesions in the front part of the cell. The actin network segregates into lamellipodium and lamellum, whereas the adhesion complexes are characteristically distributed underneath the actin system. Here, we suggest a computational model for...

journal_title：Biophysical journal

authors： Shemesh T,Bershadsky AD,Kozlov MM

更新日期：2012-04-18 00:00:00

abstract：:Using both analytical solutions obtained from simplified systems and numerical results from more realistic cases, we investigate the role played by the dielectric constant of membrane proteins epsilon(p) and pore water epsilon(w) in permeation of ions across channels. We show that the boundary and its curvature are th...

journal_title：Biophysical journal

authors： Baştuğ T,Kuyucak S

更新日期：2003-05-01 00:00:00

abstract：:Ionic channels bathed in mixed solutions of two permeant electrolytes often conduct less current than channels bathed in pure solutions of either. For many years, this anomalous mole fraction effect (AMFE) has been thought to occur only in single-file pores containing two or more ions at a time. Most thinking about ch...

journal_title：Biophysical journal

authors： Nonner W,Chen DP,Eisenberg B

更新日期：1998-05-01 00:00:00

abstract：:The CLC family of chloride channels and transporters is a functionally diverse group of proteins important in a wide range of physiological processes. ClC-4 and ClC-5 are localized to endosomes and seem to play roles in the acidification of these compartments. These proteins were recently shown to function as Cl(-)/H(...

journal_title：Biophysical journal

authors： Osteen JD,Mindell JA

更新日期：2008-11-15 00:00:00

abstract：:Recent experiments (1,2) have shown that hydrophobic ions adsorbed to lipid membranes repel each other significantly at densities as low as one charge every few tens of square nanometers. This paper shows how to calculate the mutual repulsion of a population of such ions, assumed to be discrete but free to diffuse lat...

journal_title：Biophysical journal

authors： Tsien RY

更新日期：1978-11-01 00:00:00

abstract：:The GroE chaperonin system facilitates protein folding in an ATP-dependent manner. It has remained unclear why some proteins are obligate clients of the GroE system, whereas other closely related proteins are able to fold efficiently in its absence. Factors that cause folding to be slower affect kinetic partitioning b...

journal_title：Biophysical journal

authors： Bandyopadhyay B,Mondal T,Unger R,Horovitz A

更新日期：2019-01-08 00:00:00

abstract：:We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force...

journal_title：Biophysical journal

authors： Ounkomol C,Yamada S,Heinrich V

更新日期：2010-12-15 00:00:00

abstract：:Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interr...

journal_title：Biophysical journal

authors： Otto MR,Lillo MP,Beechem JM

更新日期：1994-12-01 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00

abstract：:Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the combination of SXT with cryogenic confocal fluorescence tomography (CFT). This correlative approach allows the incorporation of molecular lo...

journal_title：Biophysical journal

authors： Smith EA,McDermott G,Do M,Leung K,Panning B,Le Gros MA,Larabell CA

更新日期：2014-10-21 00:00:00

abstract：:The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside living Bacillu...

journal_title：Biophysical journal

authors： Olshausen PV,Defeu Soufo HJ,Wicker K,Heintzmann R,Graumann PL,Rohrbach A

更新日期：2013-09-03 00:00:00

abstract：:The photocycle of the photophobic receptor sensory rhodopsin II from N. pharaonis was analyzed by varying measuring wavelengths, temperature, and pH, and by exchanging H2O with D2O. The data can be satisfactorily modeled by eight exponents over the whole range of modified parameters. The kinetic data support a model s...

journal_title：Biophysical journal

authors： Chizhov I,Schmies G,Seidel R,Sydor JR,Lüttenberg B,Engelhard M

更新日期：1998-08-01 00:00:00

abstract：:We have identified two kinetically distinct modes of block, by lidocaine, of cardiac sodium channels, activated by batrachotoxin and incorporated into planar lipid bilayers. Here, we analyze the slow blocking mode which appears as a series of nonconducting events that increase in frequency and duration with increasing...

journal_title：Biophysical journal

authors： Zamponi GW,Doyle DD,French RJ

更新日期：1993-07-01 00:00:00

abstract：:The electrostatic free energies of native and acetylated lysozymes were computed by the fixed charge model (Tanford, C., and J. G. Kirkwood, 1957, J. Am. Chem. Soc., 79:5333-5339). For each computation, the charges were transformed into a sphere of fixed radius without changing their depths or the distance between cha...

journal_title：Biophysical journal

authors： Imoto T

更新日期：1983-12-01 00:00:00

abstract：:Members of the spectrin superfamily of proteins contain different numbers of homologous repeats arranged in tandem. Each of these consists of a three-alpha-helix motif, comprising two similarly and one oppositely directed alpha-helical segment joined by nonhelical linkers of characteristic length. The right-handed alp...

journal_title：Biophysical journal

authors： Parry DA,Dixon TW,Cohen C

更新日期：1992-04-01 00:00:00

abstract：:Many proteins are anchored to lipid bilayer membranes through a combination of hydrophobic and electrostatic interactions. In the case of the membrane-bound nonreceptor tyrosine kinase Src from Rous sarcoma virus, these interactions are mediated by an N-terminal myristoyl chain and an adjacent cluster of six basic ami...

journal_title：Biophysical journal

authors： Scheidt HA,Klingler J,Huster D,Keller S

更新日期：2015-08-04 00:00:00

abstract：:We describe a puffing method for changing solutions near one surface of lipid bilayers that allows simultaneous measurement of channel activity and extent of solution change at the bilayer surface. Ion adsorption to the lipid headgroups and screening of the bilayer surface charge by mobile ions provided a convenient p...

journal_title：Biophysical journal

authors： Laver DR,Curtis BA

更新日期：1996-08-01 00:00:00