Abstract:
:We present a new approach to probing single-particle dynamics that uses dynamic light scattering from a localized region. By scattering a focused laser beam from a micron-size particle, we measure its spatial fluctuations via the temporal autocorrelation of the scattered intensity. We demonstrate the applicability of this approach by measuring the three-dimensional force constants of a single bead and a pair of beads trapped by laser tweezers. The scattering equations that relate the scattered intensity autocorrelation to the particle position correlation function are derived. This technique has potential applications for measurement of biomolecular force constants and probing viscoelastic properties of complex media.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Meller A,Bar-Ziv R,Tlusty T,Moses E,Stavans J,Safran SAdoi
10.1016/S0006-3495(98)77866-Xsubject
Has Abstractpub_date
1998-03-01 00:00:00pages
1541-8issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(98)77866-Xjournal_volume
74pub_type
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