Role of macromolecular depletion in red blood cell adhesion.

Abstract:

:The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference reflection microscopy. Our experimental results indicate that adhesion can be induced in the presence of dextran with a molecular mass >or=70 kDa and that the induced forces are strong enough to significantly suppress membrane undulations. The overall dependence of the adhesion energies on the polymer concentration is consistent with the assumption that macromolecular depletion induces this attractive interaction. In conclusion, our results indicate that depletion interaction might play a significant role in RBC adhesion via initiating close contacts, and thus suggest the importance of depletion forces for RBC interactions and its relevance to a wide variety of in vitro and in vivo cell-cell and cell-surface interactions.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Zhang ZW,Neu B

doi

10.1016/j.bpj.2009.06.006

subject

Has Abstract

pub_date

2009-08-19 00:00:00

pages

1031-7

issue

4

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(09)01061-3

journal_volume

97

pub_type

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