Thrust and Power Output of the Bacterial Flagellar Motor: A Micromagnetic Tweezers Approach.

abstract：:We have used optical tweezers to study the elasticity of red cell membranes; force was applied to a bead attached to a permeabilized spherical ghost and the force-extension relation was obtained from the response of a second bead bound at a diametrically opposite position. Interruption of the skeletal network by disso...

journal_title：Biophysical journal

authors： Sleep J,Wilson D,Simmons R,Gratzer W

更新日期：1999-12-01 00:00:00

abstract：:The hyperpolarization-activated cyclic nucleotide-modulated (HCN) cation channels are opened by membrane hyperpolarization, while their activation is modulated by the binding of cyclic adenosine monophosphate (cAMP) in the cytoplasm. Here we investigate the molecular basis of cAMP channel modulation by performing mole...

journal_title：Biophysical journal

authors： Berrera M,Pantano S,Carloni P

更新日期：2006-05-15 00:00:00

abstract：:Sodium perchlorate salt (NaClO(4)) is commonly used as an internal intensity standard in ultraviolet resonance Raman (UVRR) spectroscopy experiments. It is well known that NaClO(4) can have profound effects on peptide stability. The impact of NaClO(4) on protein stability in UVRR experiments has not yet been fully inv...

journal_title：Biophysical journal

authors： Asciutto EK,General IJ,Xiong K,Asher SA,Madura JD

更新日期：2010-01-20 00:00:00

abstract：:Lateral tension in cell plasma membranes plays an essential role in regulation of a number of membrane-related intracellular processes and cell motion. Understanding the physical factors generating the lateral tension and quantitative determination of the tension distribution along the cell membrane is an emerging top...

journal_title：Biophysical journal

authors： Schweitzer Y,Lieber AD,Keren K,Kozlov MM

更新日期：2014-01-07 00:00:00

abstract：:The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with MS2-GFP tracked in real time in live Escherichia col...

journal_title：Biophysical journal

authors： Lampo TJ,Stylianidou S,Backlund MP,Wiggins PA,Spakowitz AJ

更新日期：2017-02-07 00:00:00

abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

journal_title：Biophysical journal

authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

更新日期：1979-02-01 00:00:00

abstract：:In two-color fluorescence correlation spectroscopy (TCFCS), the fluorescence intensities of two fluorescently-labeled species are cross-correlated over time and can be used to identify static and dynamic interactions. Generally, fluorophore labels are chosen that do not undergo Förster resonance energy transfer (FRET)...

journal_title：Biophysical journal

authors： Hom EF,Verkman AS

更新日期：2002-07-01 00:00:00

abstract：:Density-gradient centrifugation of disintegrated cells from a variety of plants gave two kinds of chlorophyll particles from all except the blue-green algae. As in previous procedures using detergents, the lighter Fraction 1 particles usually had greater absorption at longer wavelengths; they always had a lower ratio ...

journal_title：Biophysical journal

authors： Brown JS

更新日期：1969-12-01 00:00:00

abstract：:Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...

journal_title：Biophysical journal

authors： Hamaneh MB,Zhang L,Buck M

更新日期：2011-07-06 00:00:00

abstract：:The effect of ionic strength on the macroscopic and microscopic redox potentials and the heme environment of cytochrome c3 from Desulfovibrio vulgaris Miyazaki F have been investigated by NMR and electrochemical methods. The redox potentials of this tetraheme protein are found to be ionic strength-dependent. Especiall...

journal_title：Biophysical journal

authors： Ohmura T,Nakamura H,Niki K,Cusanovich MA,Akutsu H

更新日期：1998-09-01 00:00:00

abstract：:We have used confocal microscopy to examine the [Ca2+]i increase in the albino eggs of the frog Xenopus laevis after fertilization. Eggs were placed in agar wells with their animal poles downward so that fertilization occurred preferentially in the equatorial plane, and confocal microscopy was used to provide a two-di...

journal_title：Biophysical journal

authors： Fontanilla RA,Nuccitelli R

更新日期：1998-10-01 00:00:00

abstract：:Charge movement currents (IQ) and calcium transients (delta[Ca2+]) were measured simultaneously in frog skeletal muscle fibers, voltage clamped in a double vaseline gap chamber, using Antipyrylazo III as the calcium indicator. The rate of release of calcium from the SR (Rrel) was calculated from the calcium transients...

journal_title：Biophysical journal

authors： Simon BJ,Hill DA

更新日期：1992-05-01 00:00:00

abstract：:Understanding cellular membrane processes is critical for the study of events such as viral entry, neurotransmitter exocytosis, and immune activation. Supported lipid bilayers are commonly used to model these membrane processes experimentally. Despite the relative simplicity of such a system, many important structural...

journal_title：Biophysical journal

authors： Kasson PM,Pande VS

更新日期：2004-06-01 00:00:00

abstract：:Some plant leaves display complex, orientation-dependent, proton nuclear magnetic resonance (1H NMR) spectra. The spectral patterns vary as the angle between the leaf surface and the applied magnetic field is varied. They also vary with temperature and with the quantity of absorbed manganous ions, but they are indepen...

journal_title：Biophysical journal

authors： McCain DC,Markley JL

更新日期：1985-11-01 00:00:00

abstract：:Isolated sarcoplasmic reticulum (SR) vesicles with polymerized calcium pump protein were freeze-dried and rotary shadowed following uranyl acetate stabilization. This technique allows direct observation of a single side of the vesicle without requiring optical filtering. The heads of individual ATPase molecules, proje...

journal_title：Biophysical journal

authors： Ferguson DG,Franzini-Armstrong C,Castellani L,Hardwicke PM,Kenney LJ

更新日期：1985-10-01 00:00:00

abstract：:The interpretation of phi-values has led to an understanding of the folding transition state ensemble of a variety of proteins. Although the main guidelines and equations for calculating phi are well established, there remains some controversy about the quality of the numerical values obtained. By analyzing a complete...

journal_title：Biophysical journal

authors： Cobos ES,Candel AM,Martinez JC

更新日期：2008-06-01 00:00:00

abstract：:VP16, a protein encoded by herpes simplex virus, has a well-characterized 78 amino acid acidic activation domain. When tethered to DNA, tandem repeats of an eight amino acid motif taken from this region stimulate the transcription of a nearby gene. This work addresses how these minimal activation motifs interact with ...

journal_title：Biophysical journal

authors： Bamdad C

更新日期：1998-10-01 00:00:00

abstract：:Kv1 potassium channels are widely distributed in mammalian tissues and are involved in a variety of functions from controlling the firing rate of neurons to maturation of T-lymphocytes. Here we show that the newly described KCNE4 beta-subunit has a drastic inhibitory effect on currents generated by Kv1.1 and Kv1.3 pot...

journal_title：Biophysical journal

authors： Grunnet M,Rasmussen HB,Hay-Schmidt A,Rosenstierne M,Klaerke DA,Olesen SP,Jespersen T

更新日期：2003-09-01 00:00:00

abstract：:Actin polymerization is typically initiated at specific sites in a cell by membrane-bound protein complexes, and the resulting structures are involved in specialized cellular functions, such as migration, particle uptake, or mitotic division. Here we analyze the potential of the actin system to self-organize into wave...

journal_title：Biophysical journal

authors： Bretschneider T,Anderson K,Ecke M,Müller-Taubenberger A,Schroth-Diez B,Ishikawa-Ankerhold HC,Gerisch G

更新日期：2009-04-08 00:00:00

abstract：:The composition and electrolyte concentration of the aqueous bathing environment have important consequences for many biological processes and can profoundly affect the behavior of biomolecules. Nevertheless, because of computational limitations, many molecular simulations of biophysical systems can be performed only ...

journal_title：Biophysical journal

authors： Zavadlav J,Sablić J,Podgornik R,Praprotnik M

更新日期：2018-05-22 00:00:00

abstract：:It is shown that alpha-helical content of eleven proteins is well correlated with alanine plus leucine content. These residues, taken singly or together, are to a first approximation randomly distributed in the four proteins whose tertiary structures have been determined (i.e., myoglobin, lysozyme, ribonuclease, alpha...

journal_title：Biophysical journal

authors： Prothero JW

更新日期：1968-11-01 00:00:00

abstract：:The prediction of absolute ligand-receptor binding affinities is essential in a wide range of biophysical queries, from the study of protein-protein interactions to structure-based drug design. End-point free energy methods, such as the Molecular Mechanics Poisson-Boltzmann Surface Area (MM/PBSA) model, have received ...

journal_title：Biophysical journal

authors： Swanson JM,Henchman RH,McCammon JA

更新日期：2004-01-01 00:00:00

abstract：:Differentiation from a multipotent stem or progenitor state to a mature cell is an essentially irreversible process. The associated changes in gene expression patterns exhibit time-directionality. This "arrow of time" in the collective change of gene expression across multiple stable gene expression patterns (attracto...

journal_title：Biophysical journal

authors： Wang J,Xu L,Wang E,Huang S

更新日期：2010-07-07 00:00:00

abstract：:Filopodia perform cellular functions such as environmental sensing or cell motility, but they also grab for particles and withdraw them leading to an increased efficiency of phagocytic uptake. Remarkably, withdrawal of micron-sized particles is also possible without noticeable movements of the filopodia. Here, we demo...

journal_title：Biophysical journal

authors： Kohler F,Rohrbach A

更新日期：2015-05-05 00:00:00

abstract：:In ubiquitination, cullin-RING E3 ubiquitin ligases (CRLs) assist in ubiquitin transfer from ubiquitin-conjugating enzyme E2 to the substrate. Neddylation, which involves NEDD8 transfer from E2 to E3-cullin, stimulates ubiquitination by inducing conformational change in CRLs. However, deneddylation, which removes NEDD...

journal_title：Biophysical journal

authors： Liu J,Nussinov R

更新日期：2010-08-04 00:00:00

abstract：:Membrane potential of aortic endothelial cells under resting conditions is dominated by inward-rectifier K(+) channels belonging to the Kir 2 family. Regulation of endothelial Kir by membrane cholesterol was studied in bovine aortic endothelial cells by altering the sterol composition of the cell membrane. Our results...

journal_title：Biophysical journal

authors： Romanenko VG,Rothblat GH,Levitan I

更新日期：2002-12-01 00:00:00

abstract：:The diffusive motion of DNA-containing chromatin in live cells and isolated nuclei is investigated using a two-photon standing wave fluorescence photobleaching experiment with 100 nm spatial resolution. The chromatin is labeled using the minor groove binding dye Hoechst 33342. In live cells, the mean diffusion rate is...

journal_title：Biophysical journal

authors： Davis SK,Bardeen CJ

更新日期：2004-01-01 00:00:00

abstract：:We present in this report a detailed structural study of calf gamma-crystallins both in the solid state and in solution by the newly developed technique of near-infrared (IR) Fourier transform (FT)-Raman spectroscopy as well as by the conventional Raman method. In comparison with conventional laser Raman spectroscopy,...

journal_title：Biophysical journal

authors： Chen WL,Nie SM,Kuck JF Jr,Yu NT

更新日期：1991-08-01 00:00:00

abstract：:The coupling between the depolymerization of microtubules (MTs) and the motion of the Dam1 ring complex is now thought to play an important role in the generation of forces during mitosis. Our current understanding of this motion is based on a number of detailed computational models. Although these models realize poss...

journal_title：Biophysical journal

authors： Armond JW,Turner MS

更新日期：2010-04-21 00:00:00

abstract：:Giant vesicles formed of 1,2-dipalmitoylphosphatidylcholine (DPPC) and sterols (cholesterol or ergosterol) in water and water/ethanol solutions have been used to examine the effect of sterol composition and ethanol concentration on the area compressibility modulus (K(a)), overall mechanical behavior, vesicle morpholog...

journal_title：Biophysical journal

authors： Tierney KJ,Block DE,Longo ML

更新日期：2005-10-01 00:00:00