Surfing along Filopodia: A Particle Transport Revealed by Molecular-Scale Fluctuation Analyses.

abstract：:Are protein nonpolar cavities filled with water molecules? Although many experimental and theoretical investigations have been done, particularly for the nonpolar cavity of IL-1 beta, the results are still conflicting. To study this problem from the thermodynamic point of view, we calculated hydration free energies of...

journal_title：Biophysical journal

authors： Oikawa M,Yonetani Y

更新日期：2010-06-16 00:00:00

abstract：:Collagenase H (ColH) from Clostridium histolyticum is a multimodular protein composed of a collagenase module (activator and peptidase domains), two polycystic kidney disease-like domains, and a collagen-binding domain. The interdomain conformation and its changes are very important for understanding the functions of ...

journal_title：Biophysical journal

authors： Ohbayashi N,Matsumoto T,Shima H,Goto M,Watanabe K,Yamano A,Katoh Y,Igarashi K,Yamagata Y,Murayama K

更新日期：2013-04-02 00:00:00

abstract：:The mechanosensitive channel of large conductance (MscL) belongs to a family of transmembrane channel proteins in bacteria and functions as a safety valve that relieves the turgor pressure produced by osmotic downshock. MscL gating can be triggered solely by stretching of the membrane. This work reports an effort to u...

journal_title：Biophysical journal

authors： Jeon J,Voth GA

更新日期：2008-05-01 00:00:00

abstract：:We have measured the static magnetization of unreduced and reduced reaction centers that vary in their quinone content. Measurements were performed in the temperature range 0.7 degrees K less than T less than 200 degrees K and magnetic fields of up to 10 kG. The electronic g-value, crystal field parameters D, E, and t...

journal_title：Biophysical journal

authors： Butler WF,Johnston DC,Shore HB,Fredkin DR,Okamura MY,Feher G

更新日期：1980-12-01 00:00:00

abstract：:Octyl-beta-thioglucopyranoside (octyl thioglucoside, OTG) is a nonionic surfactant used for the purification, reconstitution, and crystallization of membrane proteins. The thermodynamic properties of the OTG-membrane partition equilibrium are not known and have been investigated here with high-sensitivity titration ca...

journal_title：Biophysical journal

authors： Wenk MR,Seelig J

更新日期：1997-11-01 00:00:00

abstract：:Analysis of trajectories of dynamical biological objects, such as breeding ants or cell organelles, is essential to reveal the interactions they develop with their environments. Many previous works used a global characterization based on parameters calculated for entire trajectories. In cases where transient behavior ...

journal_title：Biophysical journal

authors： Huet S,Karatekin E,Tran VS,Fanget I,Cribier S,Henry JP

更新日期：2006-11-01 00:00:00

abstract：:Multiple nanosecond duration molecular dynamics simulations were performed on the transmembrane region of the Torpedo nicotinic acetylcholine receptor embedded within a bilayer mimetic octane slab. The M2 helices and M2-M3 loop regions were free to move, whereas the outer (M1, M3, M4) helix bundle was backbone restrai...

journal_title：Biophysical journal

authors： Hung A,Tai K,Sansom MS

更新日期：2005-05-01 00:00:00

abstract：:The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat and ventricular atrial myocytes. The resolution of the ...

journal_title：Biophysical journal

authors： Chen-Izu Y,McCulle SL,Ward CW,Soeller C,Allen BM,Rabang C,Cannell MB,Balke CW,Izu LT

更新日期：2006-07-01 00:00:00

abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

journal_title：Biophysical journal

authors： Dickinson GD,Parker I

更新日期：2013-01-22 00:00:00

abstract：:Directly measured forces between DNA helices in ordered arrays have been reduced to simple force coefficients and mathematical expressions for the interactions between pairs of molecules. The tabulated force parameters and mathematical expressions can be applied to parallel molecules or, by transformation, to skewed m...

journal_title：Biophysical journal

authors： Podgornik R,Rau DC,Parsegian VA

更新日期：1994-04-01 00:00:00

abstract：:It is known that the action of general anesthetics is proportional to their partition coefficient in lipid membranes (Meyer-Overton rule). This solubility is, however, directly related to the depression of the temperature of the melting transition found close to body temperature in biomembranes. We propose a thermodyn...

journal_title：Biophysical journal

authors： Heimburg T,Jackson AD

更新日期：2007-05-01 00:00:00

abstract：:The effects of chemical modifications of myosin's reactive cysteines on actomyosin adenosine triphosphatase (ATPase) activities and sliding velocities in the in vitro motility assays were examined in this work. The three types of modifications studied were 4-[N-[(iodoacetoxy)ethyl]-N-methylamino]-7-nitrobenz-2-oxa-1,3...

journal_title：Biophysical journal

authors： Root DD,Reisler E

更新日期：1992-09-01 00:00:00

abstract：:Many organs adapt to their mechanical environment as a result of physiological change or disease. Cells are both the detectors and effectors of this process. Though many studies have been performed in vitro to investigate the mechanisms of detection and adaptation to mechanical strains, the cellular strains remain unk...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-08-01 00:00:00

abstract：:The bacterial flagellar motor (BFM) is a molecular machine that rotates the helical filaments and propels the bacteria swimming toward favorable conditions. In our previous works, we built a stochastic conformational spread model to explain the dynamic and cooperative behavior of BFM switching. Here, we extended this ...

journal_title：Biophysical journal

authors： Ma Q,Sowa Y,Baker MA,Bai F

更新日期：2016-03-29 00:00:00

abstract：:Over the last decade, functionally designed DNA nanostructures applied to lipid membranes prompted important achievements in the fields of biophysics and synthetic biology. Taking advantage of the universal rules for self-assembly of complementary oligonucleotides, DNA has proven to be an extremely versatile biocompat...

journal_title：Biophysical journal

authors： Czogalla A,Franquelim HG,Schwille P

更新日期：2016-04-26 00:00:00

abstract：:The Green's function technique is applied to a study of breathing modes in a DNA double helix which contains a region of different base pairs from the rest of the double helix. The calculation is performed on a G-C helix in the B conformation with four consecutive base pairs replaced by A-T. The average stretch in hyd...

journal_title：Biophysical journal

authors： Feng Y,Prohofsky EW

更新日期：1990-03-01 00:00:00

abstract：:Investigations into the spatiotemporal dynamics of DNA repair using live-cell imaging are aided by the ability to generate well defined regions of ultravioletlike photolesions in an optical microscope. We demonstrate that multiphoton excitation of DNA in live cells with visible femtosecond pulses produces thymine cycl...

journal_title：Biophysical journal

authors： Daddysman MK,Fecko CJ

更新日期：2011-11-02 00:00:00

abstract：:Extraction from simulations of the area compressibility moduli of the monolayers in a bilayer is considered theoretically. A statistical mechanical derivation shows that the bilayer modulus is the sum of the two monolayer moduli, as is often supposed but contrary to a recent study. Seemingly plausible assumptions rega...

journal_title：Biophysical journal

authors： Nagle JF

更新日期：2019-09-17 00:00:00

abstract：:Mycolactone, a cytotoxic and immunosuppressive macrolide produced by Mycobacterium ulcerans, is the central virulent factor in the skin disease Buruli ulcer. This multifunctional cytotoxin affects fundamental cellular processes such as cell adhesion, immune response, and cell death by targeting various cellular struct...

journal_title：Biophysical journal

authors： Aydin F,Sun R,Swanson JMJ

更新日期：2019-07-09 00:00:00

abstract：:Atomic force microscopy (AFM) can be used to probe the mechanics of molecular recognition between surfaces. In the application known as "chemical force" microscopy (CFM), a chemically modified AFM tip probes a surface through chemical recognition. When modified with a biological ligand or receptor, the AFM tip can dis...

journal_title：Biophysical journal

authors： Mazzola LT,Frank CW,Fodor SP,Mosher C,Lartius R,Henderson E

更新日期：1999-06-01 00:00:00

abstract：:Sulfolobus solfataricus carboxypeptidase (CPSso) is a thermostable zinc-metalloenzyme with a M(r) of 43,000. Taking into account the experimentally determined zinc content of one ion per subunit, we developed two alternative 3D models, starting from the available structures of Thermoactinomyces vulgaris carboxypeptida...

journal_title：Biophysical journal

authors： Occhipinti E,Martelli PL,Spinozzi F,Corsi F,Formantici C,Molteni L,Amenitsch H,Mariani P,Tortora P,Casadio R

更新日期：2003-08-01 00:00:00

abstract：:Detection of the transition between the two myosin isoforms α- and β-myosin in living cardiomyocytes is essential for understanding cardiac physiology and pathology. In this study, the differences in symmetry of polarization spectra obtained from α- and β-myosin in various mammalian ventricles and propylthiouracil-tre...

journal_title：Biophysical journal

authors： Yuan C,Zhao X,Wang Z,Borg TK,Ye T,Khalpey ZI,Runyan RB,Shao Y,Gao BZ

更新日期：2020-03-10 00:00:00

abstract：:A 250 ps molecular dynamics simulation of the dimyristoylphosphatidylcholine (DMPC)-based lipid bilayer, including explicit water molecules, is reported. The solvent environment of the head groups and other structural properties of the bilayer have been analyzed and compared with experimental results as well as our pr...

journal_title：Biophysical journal

authors： Damodaran KV,Merz KM Jr

更新日期：1994-04-01 00:00:00

abstract：:We present elastic and inelastic incoherent neutron scattering data from a series of trehalose glasses diluted with glycerol. A strong correlation with recently published protein stability data in the same series of glasses illustrates that the dynamics at Q >or= 0.71 A(-1) and omega > 200 MHz are important to stabili...

journal_title：Biophysical journal

authors： Cicerone MT,Soles CL

更新日期：2004-06-01 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Evidence from electron microscopy indicates that the separation between adjacent membranes of the central nervous system (CNS) is less than 500 A and perhaps as small as 100-250 A. The rapid K(+) efflux associated with the neural action potential may therefore be sufficient to affect the local extracellular potassium ...

journal_title：Biophysical journal

authors： Lebovitz RM

更新日期：1970-05-01 00:00:00

abstract：:Tip links are thought to gate the mechanically sensitive transduction channels of hair cells, but how they form during development and regeneration remains mysterious. In particular, it is unclear how tip links are strung between stereocilia so that they are oriented parallel to a single axis; why their polarity is un...

journal_title：Biophysical journal

authors： Tompkins N,Spinelli KJ,Choi D,Barr-Gillespie PG

更新日期：2017-10-17 00:00:00

abstract：:The availability of very high magnetic fields of up to 170,000 gauss made it worthwhile to pursue the search for a critical change in the rate of four enzyme substrate reactions. The four enzymes were ribonuclease, polyphenol oxidase, peroxidase, and aldolase. The experiments showed that, to within +/-3%, no detectabl...

journal_title：Biophysical journal

authors： Rabinovitch B,Maling JE,Weissbluth M

更新日期：1967-03-01 00:00:00

abstract：:Evidence is presented for the existence of two forms of low-potential cytochrome a3. One appears to be similar to the low-spin form reported by Nicholls, P., and V. Hildebrandt (1978 Biochem. J. 173:65-72) and Wrigglesworth, J. M., J. Elsden, A. Chapman, N. Van der Water, and M. F. Grahn (1988. Biochim. Biophys. Acta....

journal_title：Biophysical journal

authors： Sidhu GS,Hendler RW

更新日期：1990-06-01 00:00:00

abstract：:The Kir3.1/Kir3.4 channel is an inward rectifier, agonist-activated K(+) channel. The location of the binding site within the channel pore that coordinates polyamines (and is thus responsible for inward rectification) and the location of the gate that opens the channel in response to agonist activation is unclear. In ...

journal_title：Biophysical journal

authors： Makary SM,Claydon TW,Dibb KM,Boyett MR

更新日期：2006-06-01 00:00:00