The binomial model in fluctuation analysis of quantal neurotransmitter release.

abstract：:We elucidate the physics of protein dynamical transition via 10-100-ns molecular dynamics simulations at temperatures spanning 160-300 K. By tracking the energy fluctuations, we show that the protein dynamical transition is marked by a crossover from nonstationary to stationary processes that underlie the dynamics of ...

journal_title：Biophysical journal

authors： Okan OB,Atilgan AR,Atilgan C

更新日期：2009-10-07 00:00:00

abstract：:A new flow configuration allows a flow cytometer of high resolution and stability to be assembled from an inverted fluorescence microscope with incident illumination, a pulse photometer, and a multichannel pulse height analyzer. A nozzle produces a hydrodynamically focused sample stream in a liquid jet that id directe...

journal_title：Biophysical journal

authors： Lindmo T,Steen HB

更新日期：1979-10-01 00:00:00

abstract：:The kinetics of conformational changes of P-type ATPases necessary for the occlusion or deocclusion of transported ions are known to be sensitive to the composition of the surrounding membrane, e.g., phospholipid content, mole percentage of cholesterol, and the presence of lipid-bound anions. Research has shown that m...

journal_title：Biophysical journal

authors： Clarke RJ

更新日期：2015-10-20 00:00:00

abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

journal_title：Biophysical journal

authors： Dickinson GD,Parker I

更新日期：2013-01-22 00:00:00

abstract：:X-ray diffraction has been applied in measuring the helical pitch of the gramicidin channel in oriented bilayers of dilauroylphosphatidylcholine (DLPC) and dimyristoylphosphatidylcholine (DMPC) at a polypeptide concentration of 9.1 mol %. The diffraction data show the helical pitch of gramicidin to be 4.7 +/- 0.2 A in...

journal_title：Biophysical journal

authors： Katsaras J,Prosser RS,Stinson RH,Davis JH

更新日期：1992-03-01 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:Cholesterol is an important risk factor of atherosclerosis, due to its active uptake by monocytes/macrophages. Monocyte recruitment from flowing blood to atherosclerotic foci is the key first step in the development of atherosclerosis. Cholesterol content alters cell membrane stiffness, and lateral lipid and protein d...

journal_title：Biophysical journal

authors： Saha AK,Osmulski P,Dallo SF,Gaczynska M,Huang TH,Ramasubramanian AK

更新日期：2017-04-11 00:00:00

abstract：:Electrorotation of fixed red blood cells has been investigated in the frequency range between 16 Hz and 30 MHz. The rotation was studied as a function of electrolyte conductivity and surface charge density. Between 16 Hz and 1 kHz, fixed red blood cells undergo cofield rotation. The maximum of cofield rotation occurs ...

journal_title：Biophysical journal

authors： Georgieva R,Neu B,Shilov VM,Knippel E,Budde A,Latza R,Donath E,Kiesewetter H,Bäumler H

更新日期：1998-04-01 00:00:00

abstract：:Investigation of the molecular mechanisms underlying amyloid-related human diseases attracts close attention. These diseases, the number of which currently is above 40, are characterized by formation of peptide or protein aggregates containing a cross-β structure. Most of the amyloidogenesis mechanisms described so fa...

journal_title：Biophysical journal

authors： Katina NS,Balobanov VA,Ilyina NB,Vasiliev VD,Marchenkov VV,Glukhov AS,Nikulin AD,Bychkova VE

更新日期：2017-09-05 00:00:00

abstract：:Elongation of the mitotic spindle during anaphase B contributes to chromosome segregation in many cells. Here, we quantitatively test the ability of two models for spindle length control to describe the dynamics of anaphase B spindle elongation using experimental data from Drosophila embryos. In the slide-and-flux-or-...

journal_title：Biophysical journal

authors： Brust-Mascher I,Civelekoglu-Scholey G,Scholey JM

更新日期：2015-04-21 00:00:00

abstract：:This is the first in a series of four papers in which we present the numerical simulation of the application of the voltage clamp technique to excitable cells. In this paper we describe the application of the Crank-Nicolson (1947) method for the solution of the parabolic partial differential equations that describe a ...

journal_title：Biophysical journal

authors： Moore JW,Ramón F,Joyner RW

更新日期：1975-01-01 00:00:00

abstract：:The brain Na+ channel-1 (BNC1, also known as MDEG1 or ASIC2) is a member of the DEG/ENaC cation channel family. Mutation of a specific residue (Gly430) that lies N-terminal to the second membrane-spanning domain activates BNC1 and converts it from a Na+-selective channel to one permeable to both Na+ and K+. Because al...

journal_title：Biophysical journal

authors： Adams CM,Price MP,Snyder PM,Welsh MJ

更新日期：1999-03-01 00:00:00

abstract：:Despite the spontaneity of some in vitro protein-folding reactions, native folding in vivo often requires the participation of barrel-shaped multimeric complexes known as chaperonins. Although it has long been known that chaperonin substrates fold upon sequestration inside the chaperonin barrel, the precise mechanism ...

journal_title：Biophysical journal

authors： England JL,Pande VS

更新日期：2008-10-01 00:00:00

abstract：:Although light microscopy and three-dimensional image analysis have made considerable progress during the last decade, it is still challenging to analyze the genome nano-architecture of specific gene domains in three-dimensional cell nuclei by fluorescence microscopy. Here, we present for the first time chromatin comp...

journal_title：Biophysical journal

authors： Hildenbrand G,Rapp A,Spöri U,Wagner C,Cremer C,Hausmann M

更新日期：2005-06-01 00:00:00

abstract：:The interaction between protein molecules and the hydroxyapatite (HAP) crystal is an important research topic in many fields. However, the nature of their noncovalent bonding is still not clear at the atomic level. In this work, molecular dynamics simulation, steered molecular dynamics simulation, and quantum chemistr...

journal_title：Biophysical journal

authors： Dong X,Wang Q,Wu T,Pan H

更新日期：2007-08-01 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation with fluorescence detection has emerged as a powerful method for the study of interacting systems of macromolecules. It combines picomolar sensitivity with high hydrodynamic resolution, and can be carried out with photoswitchable fluorophores for multicomponent dis...

journal_title：Biophysical journal

authors： Chaturvedi SK,Zhao H,Schuck P

更新日期：2017-04-11 00:00:00

abstract：:Glasses used to fabricate patch pipettes may release components which affect ion channels (Cota, G., and C.M. Armstrong. 1988. Biophys. J. 53:107-109; Furman, R.E., and J.C. Tanaka. 1988. Biophys. J. 53:287-292; Rojas, L., and C. Zuazaga. 1988. Neurosci. Lett. 88:39-44). The gating properties of maxi K+ channels from ...

journal_title：Biophysical journal

authors： Copello J,Simon B,Segal Y,Wehner F,Ramanujam VM,Alcock N,Reuss L

更新日期：1991-10-01 00:00:00

abstract：:Diluted bull semen samples were bombarded with a 24 Mev proton beam. Dose response curves for the fraction of cells which survived the bombardment and for the average velocity of the surviving cells were measured. Target theory indicated a cross section of the sensitive volume of 2.1 x 10(-10) cm(2). Respiration measu...

journal_title：Biophysical journal

authors： Rikmenspoel R,van Herpen G

更新日期：1969-06-01 00:00:00

abstract：:The triplet state absorption and phosphorescence of Zn and Pd derivatives of myoglobin were compared. Both metal derivatives exhibit long triplet state lifetimes at room temperature, but whereas the Pd derivative showed exponential decay and an isosbestic point in the transient absorption spectra, the decay of the Zn ...

journal_title：Biophysical journal

authors： Papp S,Vanderkooi JM,Owen CS,Holtom GR,Phillips CM

更新日期：1990-07-01 00:00:00

abstract：:Autoregulatory feedback loops are one of the most common network motifs. A wide variety of stochastic models have been constructed to understand how the fluctuations in protein numbers in these loops are influenced by the kinetic parameters of the main biochemical steps. These models differ according to 1) which subce...

journal_title：Biophysical journal

authors： Holehouse J,Cao Z,Grima R

更新日期：2020-04-07 00:00:00

abstract：:Cell adhesion to extracellular matrix is mediated by receptor-ligand interactions. When a cell first contacts a surface, it spreads, exerting traction forces against the surface and forming new bonds as its contact area expands. Here, we examined the changes in shape, actin polymerization, focal adhesion formation, an...

journal_title：Biophysical journal

authors： Reinhart-King CA,Dembo M,Hammer DA

更新日期：2005-07-01 00:00:00

abstract：:Light-induced dielectrophoretic movement of polystyrene beads and lambda-DNA is studied using thin films of amorphous hydrogenated silicon as local photoaddressable electrodes with a diameter of 4 microm. Positive (high-field seeking) dielectrophoretic movement is observed for both types of objects. The absence of str...

journal_title：Biophysical journal

authors： Hoeb M,Rädler JO,Klein S,Stutzmann M,Brandt MS

更新日期：2007-08-01 00:00:00

abstract：:Amyloid fibrils are associated with many neurodegenerative diseases. It was found that amyloidogenic oligomers, not mature fibrils, are neurotoxic agents related to these diseases. Molecular mechanisms of infectivity, pathways of aggregation, and molecular structure of these oligomers remain elusive. Here, we use all-...

journal_title：Biophysical journal

authors： Blinov N,Dorosh L,Wishart D,Kovalenko A

更新日期：2010-01-20 00:00:00

abstract：:The genetic map of T4 (and T2) bacteriophage is circular but the DNA molecule that is liberated by phenol extraction is a linear duplex of polynucleotide chains. If the genetic map is related to the physical structure of the DNA molecule, the problem arises as to how a linear molecule can give rise to a circular map. ...

journal_title：Biophysical journal

authors： THOMAS CA Jr,RUBENSTEIN I

更新日期：1964-03-01 00:00:00

abstract：:The interaction between actin and myosin in the filament array of glycerinated muscle fibers has been monitored using paramagnetic probes and mechanical measurements. Both fiber stiffness and the spectra of probes bound to a reactive sulfydral on the myosin head were measured as the actomyosin bond was weakened by add...

journal_title：Biophysical journal

authors： Pate E,Cooke R

更新日期：1988-04-01 00:00:00

abstract：:The results of variable dielectric coefficient Poisson-Boltzmann calculations of the counter-ion concentration in the vicinity of an all-atom model of the B-form of DNA are presented with an emphasis on the importance of spatial variations in the dielectric properties of the solvent, particularly at the macro-ion-solv...

journal_title：Biophysical journal

authors： Pack GR,Garrett GA,Wong L,Lamm G

更新日期：1993-10-01 00:00:00

abstract：:ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

journal_title：Biophysical journal

authors： Cherepanov DA,Junge W

更新日期：2001-09-01 00:00:00

abstract：:Multiple data are available on the self-assembly of mixtures of bilayer-forming amphiphiles, particularly phospholipids and micelle-forming amphiphiles, commonly denoted detergents. The structure of such mixed assemblies has been thoroughly investigated, described in phase diagrams, and theoretically rationalized in t...

journal_title：Biophysical journal

authors： Lichtenberg D,Ahyayauch H,Goñi FM

更新日期：2013-07-16 00:00:00

abstract：:Several hundred solid state NMR dipolar couplings and chemical shift anisotropies were simulated for the polytopic membrane protein, bacteriorhodopsin, and for an idealized transmembrane peptide conforming to several different secondary structures (alpha- and 3(10)-helices and parallel and antiparallel beta-sheets), e...

journal_title：Biophysical journal

authors： Sanders CR 2nd,Schwonek JP

更新日期：1993-10-01 00:00:00

abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...

journal_title：Biophysical journal

authors： Butler WL

更新日期：1972-07-01 00:00:00