Dipole-Potential-Mediated Effects on Ion Pump Kinetics.

abstract：:Eukaryotic genomes are hierarchically organized into protein-DNA assemblies for compaction into the nucleus. Nucleosomes, with the (H3-H4)2 tetrasome as a likely intermediate, are highly dynamic in nature by way of several different mechanisms. We have recently shown that tetrasomes spontaneously change the direction ...

journal_title：Biophysical journal

authors： Ordu O,Lusser A,Dekker NH

更新日期：2019-12-03 00:00:00

abstract：:Inactivation is a fundamental property of voltage-gated ion channels. Fast inactivation of Na(+) channels involves channel block by the III-IV cytoplasmic interdomain linker. The mechanisms of nonfast types of inactivation (intermediate, slow, and ultraslow) are unclear, although the ionic environment and P-loops rear...

journal_title：Biophysical journal

authors： Tikhonov DB,Zhorov BS

更新日期：2007-09-01 00:00:00

abstract：:The interaction between two stiff parallel DNA molecules is discussed using linear Debye-Hückel screening theory with and without inclusion of the dielectric discontinuity at the DNA surface, taking into account the helical symmetry of DNA. The pair potential furthermore includes the amount and distribution of counter...

journal_title：Biophysical journal

authors： Harreis HM,Likos CN,Löwen H

更新日期：2003-06-01 00:00:00

abstract：:Thermal inactivation of T(4) and lambda bacteriophage shows two components of differing sensitivity are present. These cannot be interpreted as owing to nucleic acid and protein. One protein function-the inhibition of radiation-induced DNA degradation-is lost with quite different thermal kinetics. lambda heated in the...

journal_title：Biophysical journal

authors： Pollard EC,Solosko W

更新日期：1971-01-01 00:00:00

abstract：:Cartilage has a limited capacity for self-repair and focal damage can eventually lead to complete degradation of the tissue. Early diagnosis of degenerative changes in cartilage is therefore essential. Contrast agent-based computed tomography and magnetic resonance imaging provide promising tools for this purpose. How...

journal_title：Biophysical journal

authors： Shafieyan Y,Khosravi N,Moeini M,Quinn TM

更新日期：2014-07-15 00:00:00

abstract：:It is now known that the flexibility of the troponin T (TnT) tail determines thin filament conformation and hence cross-bridge cycling properties, expanding the classic structural role of TnT to a dynamic role regulating sarcomere function. Here, using transgenic mice bearing R-92W and R-92L missense mutations in card...

journal_title：Biophysical journal

authors： He H,Javadpour MM,Latif F,Tardiff JC,Ingwall JS

更新日期：2007-09-01 00:00:00

abstract：:The microsecond folding and unfolding kinetics of ovine prion proteins (ovPrP) were measured under various solution conditions. A fragment comprising residues 94-233 of the full-length ovPrP was studied for four variants with differing susceptibilities to classical scrapie in sheep. The observed biexponential unfoldin...

journal_title：Biophysical journal

authors： Chen KC,Xu M,Wedemeyer WJ,Roder H

更新日期：2011-09-07 00:00:00

abstract：:This article shows that colchicine and taxol strongly influence the kinetics of L-type Ca channels in intact cardiac cells, and it suggests a mechanism for this action. It is known that colchicine disassociates microtubules into tubulin, and that taxol stabilizes microtubules. We have found that colchicine increases t...

journal_title：Biophysical journal

authors： Galli A,DeFelice LJ

更新日期：1994-12-01 00:00:00

abstract：:The diffusion of plastoquinol and its binding to the cytochrome bf complex, which occurs during linear photosynthetic electron transport and is analogous to reaction sequences found in most energy-converting membranes, has been studied in intact thylakoid membranes. The flash-induced electron transfer between the late...

journal_title：Biophysical journal

authors： Mitchell R,Spillmann A,Haehnel W

更新日期：1990-10-01 00:00:00

abstract：:Stabilization of the lipid bilayer membrane in red blood cells by its association with an underlying membrane-associated cytoskeleton has long been recognized as critical for proper red blood cell function. One of the principal connections between skeleton and bilayer is via linkages between band 3, the integral membr...

journal_title：Biophysical journal

authors： Butler J,Mohandas N,Waugh RE

更新日期：2008-08-01 00:00:00

abstract：:For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating ...

journal_title：Biophysical journal

authors： Hustedt EJ,Smirnov AI,Laub CF,Cobb CE,Beth AH

更新日期：1997-04-01 00:00:00

abstract：:Proteins fold and function in cellular environments that are crowded with other macromolecules. As a consequence of excluded volume effects, compact folded states of proteins should be indirectly stabilized due to destabilization of extended unfolded conformations. Here, we assess the role of excluded volume in terms ...

journal_title：Biophysical journal

authors： Mikaelsson T,Adén J,Johansson LB,Wittung-Stafshede P

更新日期：2013-02-05 00:00:00

abstract：:The intracellular and interstitial potentials associated with each cell or fiber in multicellular preparations carrying a uniformly propagating wave are important for characterizing the electrophysiological behavior of the preparation and in particular, for evaluating the source contributed by each fiber. The aforemen...

journal_title：Biophysical journal

authors： Henriquez CS,Trayanova N,Plonsey R

更新日期：1988-06-01 00:00:00

abstract：:A combined experimental and theoretical study is performed on binary dilauroylphosphatidylcholine/distearoylphosphatidylcholine (DLPC/DSPC) lipid bilayer membranes incorporating bacteriorhodopsin (BR). The system is designed to investigate the possibility that BR, via a hydrophobic matching principle related to the di...

journal_title：Biophysical journal

authors： Dumas F,Sperotto MM,Lebrun MC,Tocanne JF,Mouritsen OG

更新日期：1997-10-01 00:00:00

abstract：:Xenopus oocytes express mechanosensitive (MS(XO)) channels that can be studied in excised patches of membrane with the patch-clamp technique. This study examines the steady-state kinetic gating properties of MS(XO) channels using detailed single-channel analysis. The open and closed one-dimensional dwell-time distribu...

journal_title：Biophysical journal

authors： Gil Z,Magleby KL,Silberberg SD

更新日期：2001-10-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is a sensitive and widely used technique for measuring diffusion. FCS data are conventionally modeled with a finite number of diffusing components and fit with a least-square fitting algorithm. This approach is inadequate for analyzing data obtained from highly heterogeneous...

journal_title：Biophysical journal

authors： Sengupta P,Garai K,Balaji J,Periasamy N,Maiti S

更新日期：2003-03-01 00:00:00

abstract：:The brain Na+ channel-1 (BNC1, also known as MDEG1 or ASIC2) is a member of the DEG/ENaC cation channel family. Mutation of a specific residue (Gly430) that lies N-terminal to the second membrane-spanning domain activates BNC1 and converts it from a Na+-selective channel to one permeable to both Na+ and K+. Because al...

journal_title：Biophysical journal

authors： Adams CM,Price MP,Snyder PM,Welsh MJ

更新日期：1999-03-01 00:00:00

abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

journal_title：Biophysical journal

authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

更新日期：2010-07-21 00:00:00

abstract：:The structure of the Bowman-Birk inhibitor from Vigna unguiculata seeds (BTCI) in complex with beta-trypsin was solved and refined at 1.55 A to a crystallographic R(factor) of 0.154 and R(free) of 0.169, and represents the highest resolution for a Bowman-Birk inhibitor structure to date. The BTCI-trypsin interface is ...

journal_title：Biophysical journal

authors： Barbosa JA,Silva LP,Teles RC,Esteves GF,Azevedo RB,Ventura MM,de Freitas SM

更新日期：2007-03-01 00:00:00

abstract：:The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe to determine the viscous properties of the aqueous lumen of the ER. GFP was targeted to the ER lumen of CHO cells by transient tr...

journal_title：Biophysical journal

authors： Dayel MJ,Hom EF,Verkman AS

更新日期：1999-05-01 00:00:00

abstract：:Comparison of the electron spin resonance spectra of phosphatidylcholines spin-labeled in the sn-2 chain at a position close to the polar region and close to the methyl terminus indicate that symmetrical saturated diacyl phosphatidylcholines with odd and even chain lengths from 13 to 20 C-atoms (and probably also 12 C...

journal_title：Biophysical journal

authors： Swamy MJ,Marsh D

更新日期：1995-10-01 00:00:00

abstract：:To assess if membrane diffusion could affect the kinetics of receptor recruitment at adhesive contacts, we transfected neurons with green fluorescent protein-tagged immunoglobin cell adhesion molecules of varying length (25-180 kD), and measured the lateral mobility of single quantum dots bound to those receptors at t...

journal_title：Biophysical journal

authors： Thoumine O,Saint-Michel E,Dequidt C,Falk J,Rudge R,Galli T,Faivre-Sarrailh C,Choquet D

更新日期：2005-11-01 00:00:00

abstract：:The rate of exchange of urea across the membranes of human erythrocytes (red blood cells) was quantified on the 1-s to 2-min timescale. (13)C-urea was hyperpolarized and subjected to rapid dissolution and the previously reported (partial) resolution of (13)C NMR resonances from the molecules inside and outside red blo...

journal_title：Biophysical journal

authors： Pagès G,Puckeridge M,Liangfeng G,Tan YL,Jacob C,Garland M,Kuchel PW

更新日期：2013-11-05 00:00:00

abstract：:The elastic rigidity (stiffness) of impaled motionless bull sperm flagella has been determined by a manipulatory technique which permitted direct analytical treatment of the experimental system. The effects of external ATP and ADP were measured. It was found that ATP acts as a plasticizing agent, while ADP does not. T...

journal_title：Biophysical journal

authors： Lindemann CB,Rudd WG,Rikmenspoel R

更新日期：1973-05-01 00:00:00

abstract：:The group-additive decomposition of the unfolding free energy of a protein in an osmolyte solution relative to that in water poses a fundamental paradox: whereas the decomposition describes the experimental results rather well, theory suggests that a group-additive decomposition of free energies is, in general, not va...

journal_title：Biophysical journal

authors： Tomar DS,Asthagiri D,Weber V

更新日期：2013-09-17 00:00:00

abstract：:Ion conduction across the cellular membrane requires the simultaneous opening of activation and inactivation gates of the K+ channel pore. The bacterial KcsA channel has served as a powerful system for dissecting the structural changes that are related to four major functional states associated with K+ gating. Yet, th...

journal_title：Biophysical journal

authors： van der Cruijsen EA,Prokofyev AV,Pongs O,Baldus M

更新日期：2017-01-10 00:00:00

abstract：:Time-resolved photovoltage measurements were performed with the acid-blue (bR605A) and acid-purple (bR565A) forms of bacteriorhodopsin (bR) in the time range from 25 ns to 100 s. The bR605A and bR565A pigments were formed by titration with H2SO4 in the absence and presence of 150 mM KCI, respectively. Qualitatively th...

journal_title：Biophysical journal

authors： Moltke S,Heyn MP

更新日期：1995-11-01 00:00:00

abstract：:The circadian clocks keeping time in many living organisms rely on self-sustained biochemical oscillations entrained by external cues, such as light, to the 24-h cycle induced by Earth's rotation. However, environmental cues are unreliable due to the variability of habitats, weather conditions, or cue-sensing mechanis...

journal_title：Biophysical journal

authors： Pfeuty B,Thommen Q,Lefranc M

更新日期：2011-06-08 00:00:00

abstract：:The high gradient magnetic separation technique has been applied to separate paramagnetic erythrocytes from a cell suspension that also contained diamagnetic cells. Paramagnetism was induced in the red blood cells by oxidizing the iron atoms in the cell hemoglobin to the ferric state (methemoglobin). Diamagnetic cells...

journal_title：Biophysical journal

authors： Owen CS

更新日期：1978-05-01 00:00:00

abstract：:The muscle nicotinic receptor (AChR) is a pentamer of four different subunits, each of which contains four transmembrane domains (M1-M4). We recently showed that channel opening and closing rates of the AChR depend on a hydrogen bond involving a threonine at position 14' of the M4 domain in the alpha-subunit. To deter...

journal_title：Biophysical journal

authors： Bouzat C,Gumilar F,del Carmen Esandi M,Sine SM

更新日期：2002-04-01 00:00:00