Weak effect of membrane diffusion on the rate of receptor accumulation at adhesive contacts.

abstract：:Several strategies directed toward increasing the participation of minority students in physics and biophysics are presented. Since the number of minority students entering college with an interest in science and mathematics must be increased if we expect to see more students graduating in science, several programs ai...

journal_title：Biophysical journal

authors： Tanaka JC,Gladney LD

更新日期：1993-07-01 00:00:00

abstract：:The outer membrane of Gram-negative bacteria is of great scientific interest because it mediates the action of antimicrobial agents. The membrane surface is composed of lipopolysaccharide (LPS) molecules with negatively charged oligosaccharide headgroups. To a certain fraction, LPSs additionally display linear polysac...

journal_title：Biophysical journal

authors： Micciulla S,Gerelli Y,Schneck E

更新日期：2019-04-02 00:00:00

abstract：:We present the results of a detailed molecular dynamics study of the closed form of the P2X4 receptor. The fluctuations observed in the simulations were compared with the changes that occur in the transition from the closed to the open structure. To get further insight on the opening mechanism, the actual displacement...

journal_title：Biophysical journal

authors： Pierdominici-Sottile G,Moffatt L,Palma J

更新日期：2016-12-20 00:00:00

abstract：:A mesoscale model of DNA is presented (3SPN.1), extending the scheme previously developed by our group. Each nucleotide is mapped onto three interaction sites. Solvent is accounted for implicitly through a medium-effective dielectric constant and electrostatic interactions are treated at the level of Debye-Hückel theo...

journal_title：Biophysical journal

authors： Sambriski EJ,Schwartz DC,de Pablo JJ

更新日期：2009-03-04 00:00:00

abstract：:Tissues and cells sustain recurring mechanical loads that span a wide range of loading amplitudes and timescales as a consequence of exposure to blood flow, muscle activity, and external impact. Both tissues and cells derive their mechanical strength from fibrous protein scaffolds, which typically have a complex hiera...

journal_title：Biophysical journal

authors： Kurniawan NA,Vos BE,Biebricher A,Wuite GJ,Peterman EJ,Koenderink GH

更新日期：2016-09-06 00:00:00

abstract：:We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The in...

journal_title：Biophysical journal

authors： Friedrich M,Gan Q,Ermolayev V,Harms GS

更新日期：2011-04-20 00:00:00

abstract：:The size of a cell is central to many functions, including cellular communication and exchange of materials with the environment. This modeling and experimental study focused on understanding how the size of a cell determines its ability to uptake nanometer-scale extracellular materials from the environment. Several m...

journal_title：Biophysical journal

authors： Khetan J,Shahinuzzaman M,Barua S,Barua D

更新日期：2019-01-22 00:00:00

abstract：:Fiber orientation and ventricular geometry are incorporated in a mathematical model for ejection fraction of the human left ventricle. The inadequacy of circularly oriented or "constrictor" fibers to explain high ejection fractions of cylindrical, spherical, or ellipsoidal reference ventricles is demonstrated. A class...

journal_title：Biophysical journal

authors： Sallin EA

更新日期：1969-07-01 00:00:00

abstract：:The orientational order as determined by 2H NMR and the infrared frequencies of the C--H stretching modes of the methylene groups have been measured for several systems (POPC, POPC/cholesterol and POPE), all in the fluid phase, and then were compared; this work reveals an unexpected linear correlation between them. Th...

journal_title：Biophysical journal

authors： Kodati VR,Lafleur M

更新日期：1993-01-01 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:The formation and function of the mitotic spindle depends upon force generation by multiple molecular motors and by the dynamics of microtubules, but how these force-generating mechanisms relate to one another is unclear. To address this issue we have modeled the separation of spindle poles as a function of time durin...

journal_title：Biophysical journal

authors： Cytrynbaum EN,Scholey JM,Mogilner A

更新日期：2003-02-01 00:00:00

abstract：:The effects of rigor and cycling cross-bridges on distributions of calcium (Ca) bound within sarcomeres of rabbit psoas muscle fibers were compared using electron probe x-ray microanalysis. Calcium in the overlap region of rigor fibers, after correction for that bound to thick filaments, was significantly higher than ...

journal_title：Biophysical journal

authors： Cantino ME,Quintanilla A

更新日期：2007-01-15 00:00:00

abstract：:Embryonic and adult fibroblasts can be returned to pluripotency by the expression of reprogramming genes. Multiple lines of evidence suggest that these human induced pluripotent stem (hiPS) cells and human embryonic stem (hES) cells are behaviorally, karyotypically, and morphologically similar. Here we sought to deter...

journal_title：Biophysical journal

authors： Daniels BR,Hale CM,Khatau SB,Kusuma S,Dobrowsky TM,Gerecht S,Wirtz D

更新日期：2010-12-01 00:00:00

abstract：:Chimeric antigen receptors (CARs) have recently been approved for the treatment of hematological malignancies, but our lack of understanding of the basic mechanisms that activate these proteins has made it difficult to optimize and control CAR-based therapies. In this study, we use phosphoproteomic mass spectrometry a...

journal_title：Biophysical journal

authors： Rohrs JA,Zheng D,Graham NA,Wang P,Finley SD

更新日期：2018-09-18 00:00:00

abstract：:Recent biological optical spectroscopic studies have correlated discrete spectroscopic states with biological function in several systems. One of the challenges of molecular biophysics is to correlate structural changes with these spectroscopic states. From small-angle x-ray scattering one can obtain a key structural ...

journal_title：Biophysical journal

authors： Stamatoff J

更新日期：1979-05-01 00:00:00

abstract：:The parameters of the immune response dynamics are usually estimated by the use of deterministic ordinary differential equations that relate data trends to parameter values. Since the physical basis of the response is stochastic, we are investigating the intensity of the data fluctuations resulting from the intrinsic ...

journal_title：Biophysical journal

authors： Milutinovic D,De Boer RJ

更新日期：2007-05-15 00:00:00

abstract：:The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...

journal_title：Biophysical journal

authors： Lacroix JJ,Bezanilla F

更新日期：2012-08-08 00:00:00

abstract：:Excised inside-out membrane patches are useful for studying the cGMP-activated ion channels that generate the electrical response to light in retinal rod cells. We show that strong ionic current across a patch changes the driving force on the current by altering the ionic concentration near the surface membrane, an ef...

journal_title：Biophysical journal

authors： Zimmerman AL,Karpen JW,Baylor DA

更新日期：1988-08-01 00:00:00

abstract：:Conventional equations for enzyme kinetics are based on mass-action laws, that may fail in low-dimensional and disordered media such as biological membranes. We present Monte Carlo simulations of an isolated Michaelis-Menten enzyme reaction on two-dimensional lattices with varying obstacle densities, as models of biol...

journal_title：Biophysical journal

authors： Berry H

更新日期：2002-10-01 00:00:00

abstract：:Ion channel behavior can depend strongly on temperature, with faster kinetics at physiological temperatures leading to considerable changes in currents relative to room temperature. These temperature-dependent changes in voltage-dependent ion channel kinetics (rates of opening, closing, inactivating, and recovery) are...

journal_title：Biophysical journal

authors： Lei CL,Clerx M,Beattie KA,Melgari D,Hancox JC,Gavaghan DJ,Polonchuk L,Wang K,Mirams GR

更新日期：2019-12-17 00:00:00

abstract：:A structural model is suggested for axon membranes consisting of a double layer of lipid and phospholipid molecules in which the polar ends of certain phospholipids change their orientation and combining properties under the influence of an electric field. The phosphate groups act as ion exchange "gates" for the contr...

journal_title：Biophysical journal

authors： GOLDMANN DE

更新日期：1964-05-01 00:00:00

abstract：:Multiple molecular dynamics simulations of bacterioopsin pulling from its C-terminus show that its alpha-helices unfold individually. In the first metastable state observed in the simulations, helix G is unfolded at its C-terminal segment while the rest of helix G (residues 200-216) is folded and opposes resistance be...

journal_title：Biophysical journal

authors： Seeber M,Fanelli F,Paci E,Caflisch A

更新日期：2006-11-01 00:00:00

abstract：:It is well known that the dsDNA molecule undergoes a phase transition from B-DNA into an overstretched state at high forces. For some time, the structure of the overstretched state remained unknown and highly debated, but recent advances in experimental techniques have presented evidence of more than one possible phas...

journal_title：Biophysical journal

authors： Argudo D,Purohit PK

更新日期：2014-11-04 00:00:00

abstract：:Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. ...

journal_title：Biophysical journal

authors： Alexandre MT,Domratcheva T,Bonetti C,van Wilderen LJ,van Grondelle R,Groot ML,Hellingwerf KJ,Kennis JT

更新日期：2009-07-08 00:00:00

abstract：:Microsecond rotational motions of nitroxide spin labels are measured primarily with saturation transfer electron paramagnetic resonance (ST-EPR). In the present study we have used model system experiments to quantitatively evaluate different ST-EPR spectral parameters, both in-phase and out-of-phase, with an emphasis ...

journal_title：Biophysical journal

authors： Squier TC,Thomas DD

更新日期：1986-04-01 00:00:00

abstract：:Optical mapping with voltage-sensitive dyes provides a high-resolution technique to observe cardiac electrodynamic behavior. Although most studies assume that the fluorescent signal is emitted from the surface layer of cells, the effects of signal attenuation with depth on signal interpretation are still unclear. This...

journal_title：Biophysical journal

authors： Bray MA,Wikswo JP

更新日期：2003-12-01 00:00:00

abstract：:Recent experiments (1,2) have shown that hydrophobic ions adsorbed to lipid membranes repel each other significantly at densities as low as one charge every few tens of square nanometers. This paper shows how to calculate the mutual repulsion of a population of such ions, assumed to be discrete but free to diffuse lat...

journal_title：Biophysical journal

authors： Tsien RY

更新日期：1978-11-01 00:00:00

abstract：:Structural changes in frog skeletal muscle were studied using x-ray diffraction with a time resolution of 0.53-1.02 ms after a single electrical stimulus at 8 degrees C. Tension began to drop at 6 ms (latency relaxation), reached a minimum at 8 ms, and then twitch tension developed. The intensity of the meridional ref...

journal_title：Biophysical journal

authors： Yagi N

更新日期：2003-02-01 00:00:00

abstract：:In recent in vitro experiments, it has been demonstrated that the 47-kDa fragment of the talin molecule and the 32-kDa fragment of the vinculin molecule interact with acidic phospholipids. By using a computer analysis method, we determined the hydrophobic and amphipathic stretches of these fragments and, by applying a...

journal_title：Biophysical journal

authors： Tempel M,Goldmann WH,Isenberg G,Sackmann E

更新日期：1995-07-01 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00