当前位置： SCI文献检索 > BIOPHYSICAL JOURNAL期刊下所有文献 > Methodology for increased precision in saturation transfer electron paramagnetic resonance studies of rotational dynamics.

Methodology for increased precision in saturation transfer electron paramagnetic resonance studies of rotational dynamics.

Abstract：

:Microsecond rotational motions of nitroxide spin labels are measured primarily with saturation transfer electron paramagnetic resonance (ST-EPR). In the present study we have used model system experiments to quantitatively evaluate different ST-EPR spectral parameters, both in-phase and out-of-phase, with an emphasis on techniques for suppressing the interference from weakly immobilized probes. Analyses of both systematic and random errors show that maximum sensitivity to small changes in correlation time and minimum ambiguity of interpretation are best achieved by combining measurements of both spectral line-shape, i.e., the ratio of line-heights, and spectral intensity, i.e., the absolute amplitude of either a position within a spectrum or a spectral integral. Errors in the measurement of correlation times for the two types of parameters tend to be complementary. Integrated intensity parameters are particularly useful in measuring microsecond probe motions in the presence of weakly immobilized components. We confirm that integrated intensity parameters are sometimes effective in rejecting signals from weakly immobilized probes, but the effectiveness of this rejection is more limited than previously supposed and depends on the type of parameter being measured. We describe procedures for evaluating and minimizing errors due to weakly immobilized probes, emphasizing the advantages of a new kind of intensity parameter obtained from integrated in-phase spectra. We provide detailed descriptions of experimental procedures, along with calibration plots of the most useful spectral parameters vs. rotational correlation time, which should make it possible for workers in other laboratories, using different instruments and sample geometries, to reproduce spectra quantitatively and to make accurate correlation time measurements.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Squier TC,Thomas DD

doi

10.1016/S0006-3495(86)83720-1

subject

Has Abstract

pub_date

1986-04-01 00:00:00

pages

921-35

issue

4

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(86)83720-1

journal_volume

49

pub_type

杂志文章

相关文献

BIOPHYSICAL JOURNAL文献大全
  • A delayed rectifier potassium current in Xenopus oocytes.

    abstract：:A delayed voltage-dependent K+ current endogenous to Xenopus oocytes has been investigated by the voltage-clamp technique. Both activation and inactivation of the K+ current are voltage-dependent processes. The K+ currents were activated when membrane potential was depolarized from a holding potential of -90 to -50 mV...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(90)82632-1

    authors： Lu L,Montrose-Rafizadeh C,Hwang TC,Guggino WB

    更新日期：1990-06-01 00:00:00

  • Conformational partitioning of the fusion peptide of HIV-1 gp41 and its structural analogs in bilayer membranes.

    abstract：:Experiments have shown that the ability of the HIV-1 virus to infect cells can be greatly diminished by deactivation of the N-terminal (fusion) peptide of its glycoprotein gp41. Deactivation can be achieved by the deletion of several amino acid residues, or replacement of a hydrophobic residue with a polar residue, to...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(02)75313-7

    authors： Maddox MW,Longo ML

    更新日期：2002-12-01 00:00:00

  • Molecular dynamics simulation of the structure of dimyristoylphosphatidylcholine bilayers with cholesterol, ergosterol, and lanosterol.

    abstract：:Five molecular dynamics computer simulations were performed on different phospholipid:sterol membrane systems in order to study the influence of sterol structure on membrane properties. Three of these simulated bilayer systems were composed of a 1:8 sterol:phospholipid ratio, each of which employed one of the sterol m...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(01)76137-1

    authors： Smondyrev AM,Berkowitz ML

    更新日期：2001-04-01 00:00:00

  • A molecular dynamics investigation of vinculin activation.

    abstract：:Vinculin activation plays a critical role in focal adhesion initiation and formation. In its native state, vinculin is in an autoinhibitory conformation in which domain 1 prevents interaction of the vinculin tail domain with actin by steric hindrance. Once activated, vinculin is able to interact with both actin and ta...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.05.024

    authors： Golji J,Mofrad MR

    更新日期：2010-08-09 00:00:00

  • Interaction of tea catechin (-)-epigallocatechin gallate with lipid bilayers.

    abstract：:A major component of green tea extracts, catechin (-)-Epigallocatechin gallate (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 microM caused GUVs to burst. Here we inve...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2008.11.007

    authors： Sun Y,Hung WC,Chen FY,Lee CC,Huang HW

    更新日期：2009-02-01 00:00:00

  • Surface potentials measure ion concentrations near lipid bilayers during rapid solution changes.

    abstract：:We describe a puffing method for changing solutions near one surface of lipid bilayers that allows simultaneous measurement of channel activity and extent of solution change at the bilayer surface. Ion adsorption to the lipid headgroups and screening of the bilayer surface charge by mobile ions provided a convenient p...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(96)79271-8

    authors： Laver DR,Curtis BA

    更新日期：1996-08-01 00:00:00

  • Rotational dynamics of spin-labeled F-actin during activation of myosin S1 ATPase using caged ATP.

    abstract：:The most probable source of force generation in muscle fibers in the rotation of the myosin head when bound to actin. This laboratory has demonstrated that ATP induces microsecond rotational motions of spin-labeled myosin heads bound to actin (Berger, C. L. E. C. Svensson, and D. D. Thomas. 1989. Proc. Natl. Acad. Sci...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(91)82338-4

    authors： Ostap EM,Thomas DD

    更新日期：1991-06-01 00:00:00

  • Unusual Organization of I-BAR Proteins on Tubular and Vesicular Membranes.

    abstract：:Protein-mediated membrane remodeling is a ubiquitous and critical process for proper cellular function. Inverse Bin/Amphiphysin/Rvs (I-BAR) domains drive local membrane deformation as a precursor to large-scale membrane remodeling. We employ a multiscale approach to provide the molecular mechanism of unusual I-BAR dom...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2019.06.025

    authors： Jarin Z,Tsai FC,Davtyan A,Pak AJ,Bassereau P,Voth GA

    更新日期：2019-08-06 00:00:00

  • Design Parameters for Granzyme-Mediated Cytotoxic Lymphocyte Target-Cell Killing and Specificity.

    abstract：:Cytotoxic lymphocytes are key elements of the immune system that are primarily responsible for targeting cells infected with intracellular pathogens, or cells that have become malignantly transformed. Target cells are killed mainly via lymphocyte exocytosis of specialized lysosomes containing perforin, a pore-forming ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2015.06.045

    authors： Woodsworth DJ,Dunsing V,Coombs D

    更新日期：2015-08-04 00:00:00

  • Discrimination of DNA hybridization using chemical force microscopy.

    abstract：:Atomic force microscopy (AFM) can be used to probe the mechanics of molecular recognition between surfaces. In the application known as "chemical force" microscopy (CFM), a chemically modified AFM tip probes a surface through chemical recognition. When modified with a biological ligand or receptor, the AFM tip can dis...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(99)77447-3

    authors： Mazzola LT,Frank CW,Fodor SP,Mosher C,Lartius R,Henderson E

    更新日期：1999-06-01 00:00:00

  • A procedure for refining a coiled coil protein structure using x-ray fiber diffraction and modeling.

    abstract：:We describe a combined use of experimental and simulation techniques to configure side chains in a coiled coil structure. As already demonstrated in a previous work, x-ray diffraction patterns from hard alpha-keratin fibers in the 5.15 A meridian zone reflect the global configuration of the chi(1) dihedral angle of th...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(02)73943-X

    authors： Briki F,Doucet J,Etchebest C

    更新日期：2002-10-01 00:00:00

  • Endothelin receptor dimers evaluated by FRET, ligand binding, and calcium mobilization.

    abstract：:Endothelin-1 (ET-1) mediates physiological responses via endothelin A (ET(A)) and B (ET(B)) receptors, which may form homo- and heterodimers with unknown function. Here, we investigated ET-receptor dimerization using fluorescence resonance energy transfer (FRET) between receptors tagged with CFP (donor) and receptors ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.119206

    authors： Evans NJ,Walker JW

    更新日期：2008-07-01 00:00:00

  • The Roles of Microtubules and Membrane Tension in Axonal Beading, Retraction, and Atrophy.

    abstract：:Axonal beading, or the formation of a series of swellings along the axon, and retraction are commonly observed shape transformations that precede axonal atrophy in Alzheimer's disease, Parkinson's disease, and other neurodegenerative conditions. The mechanisms driving these morphological transformations are poorly und...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2019.07.046

    authors： Datar A,Ameeramja J,Bhat A,Srivastava R,Mishra A,Bernal R,Prost J,Callan-Jones A,Pullarkat PA

    更新日期：2019-09-03 00:00:00

  • Role of Inverse-Cone-Shape Lipids in Temperature-Controlled Self-Reproduction of Binary Vesicles.

    abstract：:We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two dau...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.02.028

    authors： Jimbo T,Sakuma Y,Urakami N,Ziherl P,Imai M

    更新日期：2016-04-12 00:00:00

  • Pulsed electron spin resonance resolves the coordination site of Cu²(+) ions in α1-glycine receptor.

    abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.08.050

    authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

    更新日期：2010-10-20 00:00:00

  • Introducing titratable water to all-atom molecular dynamics at constant pH.

    abstract：:Recent development of titratable coions has paved the way for realizing all-atom molecular dynamics at constant pH. To further improve physical realism, here we describe a technique in which proton titration of the solute is directly coupled to the interconversion between water and hydroxide or hydronium. We test the ...

    journal_title：Biophysical journal

    pub_type： 信件

    doi：10.1016/j.bpj.2013.06.036

    authors： Chen W,Wallace JA,Yue Z,Shen JK

    更新日期：2013-08-20 00:00:00

  • Quantal sarcomere-length changes in relaxed single myofibrils.

    abstract：:We carried out experiments on single isolated myofibrils in which thin filaments had been functionally removed, leaving the connecting (titin) filaments as the sole agent taking up the length change. With technical advances that gave sub-nanometer detectability we examined the time course of single sarcomere-length ch...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(01)75766-9

    authors： Blyakhman F,Tourovskaya A,Pollack GH

    更新日期：2001-08-01 00:00:00

  • Critical determinants of Ca(2+)-dependent inactivation within an EF-hand motif of L-type Ca(2+) channels.

    abstract：:L-type (alpha(1C)) calcium channels inactivate rapidly in response to localized elevation of intracellular Ca(2+), providing negative Ca(2+) feedback in a diverse array of biological contexts. The dominant Ca(2+) sensor for such Ca(2+)-dependent inactivation has recently been identified as calmodulin, which appears to...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(00)76739-7

    authors： Peterson BZ,Lee JS,Mulle JG,Wang Y,de Leon M,Yue DT

    更新日期：2000-04-01 00:00:00

  • Resident Calmodulin Primes NMDA Receptors for Ca2+-Dependent Inactivation.

    abstract：:N-methyl-d-aspartate (NMDA) receptors are glutamate- and glycine-gated channels that flux Na+ and Ca2+ into postsynaptic neurons during synaptic transmission. The resulting intracellular Ca2+ transient is essential to physiological and pathological processes related to synaptic development, plasticity, and apoptosis. ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2017.06.035

    authors： Iacobucci GJ,Popescu GK

    更新日期：2017-11-21 00:00:00

  • Laser-Activated Polymeric Microcapsules for Ultrasound Imaging and Therapy: In Vitro Feasibility.

    abstract：:Polymeric microcapsules with a light-absorbing dye incorporated in their shell can generate vapor microbubbles that can be spatiotemporally controlled by pulsed laser irradiation. These contrast agents of 6-8 μm in diameter can circulate through the vasculature, offering possibilities for ultrasound (molecular) imagin...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2017.03.033

    authors： Lajoinie G,van Rooij T,Skachkov I,Blazejewski E,Veldhuis G,de Jong N,Kooiman K,Versluis M

    更新日期：2017-05-09 00:00:00

  • High Spatiotemporal-Resolution Magnetic Tweezers: Calibration and Applications for DNA Dynamics.

    abstract：:The observation of biological processes at the molecular scale in real time requires high spatial and temporal resolution. Magnetic tweezers are straightforward to implement, free of radiation or photodamage, and provide ample multiplexing capability, but their spatiotemporal resolution has lagged behind that of other...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2015.10.018

    authors： Dulin D,Cui TJ,Cnossen J,Docter MW,Lipfert J,Dekker NH

    更新日期：2015-11-17 00:00:00

  • The potential landscape of genetic circuits imposes the arrow of time in stem cell differentiation.

    abstract：:Differentiation from a multipotent stem or progenitor state to a mature cell is an essentially irreversible process. The associated changes in gene expression patterns exhibit time-directionality. This "arrow of time" in the collective change of gene expression across multiple stable gene expression patterns (attracto...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.03.058

    authors： Wang J,Xu L,Wang E,Huang S

    更新日期：2010-07-07 00:00:00

  • On the adsorption of phloretin onto a black lipid membrane.

    abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/s0006-3495(79)85292-3

    authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

    更新日期：1979-02-01 00:00:00

  • Imaging the Nonlinear Susceptibility Tensor of Collagen by Nonlinear Optical Stokes Ellipsometry.

    abstract：:Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen-rich biological tissues. NOSE is based on polarization-dependent second harmonic generation imaging. NOSE was used to access the molecular-level distribution of collagen fibril orientation relative to the local fiber...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.05.055

    authors： Dow XY,DeWalt EL,Sullivan SZ,Schmitt PD,Ulcickas JRW,Simpson GJ

    更新日期：2016-10-04 00:00:00

  • The Chemical Potential of Plasma Membrane Cholesterol: Implications for Cell Biology.

    abstract：:Cholesterol is abundant in plasma membranes and exhibits a variety of interactions throughout the membrane. Chemical potential accounts for thermodynamic consequences of molecular interactions, and quantifies the effective concentration (i.e., activity) of any substance participating in a process. We have developed, t...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2017.12.042

    authors： Ayuyan AG,Cohen FS

    更新日期：2018-02-27 00:00:00

  • A Model for the Hippo Pathway in the Drosophila Wing Disc.

    abstract：:Although significant progress has been made toward understanding morphogen-mediated patterning in development, control of the size and shape of tissues via local and global signaling is poorly understood. In particular, little is known about how cell-cell interactions are involved in the control of tissue size. The Hi...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2018.07.002

    authors： Gou J,Lin L,Othmer HG

    更新日期：2018-08-21 00:00:00

  • A new method to study shape recovery of red blood cells using multiple optical trapping.

    abstract：:In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell deformation, which is comparable to the deformation in small capillaries. The shape recovery is recorded, and a relaxation time is obtained for each individual red...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)80084-6

    authors： Bronkhorst PJ,Streekstra GJ,Grimbergen J,Nijhof EJ,Sixma JJ,Brakenhoff GJ

    更新日期：1995-11-01 00:00:00

  • Folding pathways of prion and doppel.

    abstract：:The relevance of various residue positions for the stability and the folding characteristics of the prion protein in its normal cellular form are investigated by using molecular dynamics simulations of models exploiting the topology of the native state. These models allow for reproducing the experimentally validated t...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(02)75353-8

    authors： Settanni G,Hoang TX,Micheletti C,Maritan A

    更新日期：2002-12-01 00:00:00

  • Electron paramagnetic resonance characterization of tyrosine radical, M+, in site-directed mutants of photosystem II(t).

    abstract：:Photosystem II contains two well-characterized tyrosine radicals, D(.) and Z(.). Z is an electron carrier between the primary chlorophyll donor and the manganese catalytic site and is essential for enzymatic function. On the other hand, D forms a stable radical with no known role in oxygen evolution. D(.) and Z(.) giv...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(96)79394-3

    authors： Ma C,Barry BA

    更新日期：1996-10-01 00:00:00

  • The functional form of angular forces around transition metal ions in biomolecules.

    abstract：:A method for generating angular forces around sigma-bonded transition metal ions is generalized to treat pi-bonded configurations. The theoretical approach is based on an analysis of ligand-field and small-cluster Hamiltonians based on the moments of the electron state distribution. The functional forms that are obtai...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(01)75675-5

    authors： Carlsson AE,Zapata S

    更新日期：2001-07-01 00:00:00