Resident Calmodulin Primes NMDA Receptors for Ca2+-Dependent Inactivation.

abstract：:Two T = 1 and one T = 3 plant viruses, along with a protein, were crystallized in microgravity during the International Microgravity Laboratory-2 (IML-2) mission in July of 1994. The method used was liquid-liquid diffusion in the European Space Agency's Advanced Protein Crystallization Facility (APCF). Distinctive alt...

journal_title：Biophysical journal

authors： Koszelak S,Day J,Leja C,Cudney R,McPherson A

更新日期：1995-07-01 00:00:00

abstract：:Many toxins and antimicrobial peptides permeabilize membrane vesicles by forming multimeric pores. Determination of the size of such pores is an important first step for understanding their structure and the mechanism of their self-assembly. We report a simple method for sizing pores in vesicles based on the different...

journal_title：Biophysical journal

authors： Ladokhin AS,Selsted ME,White SH

更新日期：1997-04-01 00:00:00

abstract：:The observation of biological processes at the molecular scale in real time requires high spatial and temporal resolution. Magnetic tweezers are straightforward to implement, free of radiation or photodamage, and provide ample multiplexing capability, but their spatiotemporal resolution has lagged behind that of other...

journal_title：Biophysical journal

authors： Dulin D,Cui TJ,Cnossen J,Docter MW,Lipfert J,Dekker NH

更新日期：2015-11-17 00:00:00

abstract：:Understanding cellular membrane processes is critical for the study of events such as viral entry, neurotransmitter exocytosis, and immune activation. Supported lipid bilayers are commonly used to model these membrane processes experimentally. Despite the relative simplicity of such a system, many important structural...

journal_title：Biophysical journal

authors： Kasson PM,Pande VS

更新日期：2004-06-01 00:00:00

abstract：:The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their composit...

journal_title：Biophysical journal

authors： Casadei BR,Domingues CC,de Paula E,Riske KA

更新日期：2014-06-03 00:00:00

abstract：:The relation between the dynamic properties of the haptenic site of lipid haptens and the phase transition of the host lattice was investigated using head group spin-labeled phosphatidylethanolamines, that is, spin-label lipid haptens (Brûlet, P., and H. M. McConnell, 1976, Proc. Natl. Acad. Sci. USA., 73:2977-2981; B...

journal_title：Biophysical journal

authors： Takeshita K,Utsumi H,Hamada A

更新日期：1987-08-01 00:00:00

abstract：:Connectin (titin) is a large filamentous protein (single peptide) with a molecular mass of approximately 3 MDa, contour length approximately 900 nm, and diameter approximately 4 nm, and resides in striated muscle. Connectin links the thick filaments to the Z-lines in a sarcomere and produces a passive elastic force wh...

journal_title：Biophysical journal

authors： Higuchi H,Nakauchi Y,Maruyama K,Fujime S

更新日期：1993-11-01 00:00:00

abstract：:Measurements of voltage relaxations following brief charge-pulses applied to lipid bilayers have been performed at different hydrostatic pressures in the presence of the neutral carriers cyclo (D-Val-L-Pro-L-Val-D-Pro)3(PV) and valinomycin. From double-exponential relaxations observed in membranes containing PV-K+ com...

journal_title：Biophysical journal

authors： Benz R,Conti F

更新日期：1986-07-01 00:00:00

abstract：:Diffusional encounters between a dumbbell model of a cleft enzyme and a dumbbell model of an elongated ligand are simulated by Brownian dynamics. The simulations take into account electrostatic and hydrodynamic interactions between the molecules. It is shown that the primary effect of inclusion of hydrodynamic interac...

journal_title：Biophysical journal

authors： Antosiewicz J,McCammon JA

更新日期：1995-07-01 00:00:00

abstract：:Structural rearrangements of the myosin upper-50 kD subdomain are thought to play a key role in coordinating actin binding with nucleotide hydrolysis during the myosin ATPase cycle. Such rearrangements could open and close the active site in opposition to the actin-binding cleft, helping explain the opposing affinitie...

journal_title：Biophysical journal

authors： Robertson CI,Gaffney DP 2nd,Chrin LR,Berger CL

更新日期：2005-09-01 00:00:00

abstract：:How calmodulin (CaM) acts in KRAS-driven cancers is a vastly important question. CaM binds to and stimulates PI3Kα/Akt signaling, promoting cell growth and proliferation. Phosphorylation of CaM at Tyr99 (pY99) enhances PI3Kα activation. PI3Kα is a lipid kinase. It phosphorylates PIP2 to produce PIP3, to which Akt bind...

journal_title：Biophysical journal

authors： Zhang M,Jang H,Gaponenko V,Nussinov R

更新日期：2017-11-07 00:00:00

abstract：:Plasma insulin measurements from mice, rats, dogs, and humans indicate that insulin levels are oscillatory, reflecting pulsatile insulin secretion from individual islets. An unanswered question, however, is how the activity of a population of islets is coordinated to yield coherent oscillations in plasma insulin. Here...

journal_title：Biophysical journal

authors： Fendler B,Zhang M,Satin L,Bertram R

更新日期：2009-08-05 00:00:00

abstract：:The [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii can catalyze the reduction of protons to hydrogen gas using electrons supplied from photosystem I and transferred via ferredoxin. To better understand the association of the hydrogenase and the ferredoxin, we have simulated the process over multiple ...

journal_title：Biophysical journal

authors： Long H,Chang CH,King PW,Ghirardi ML,Kim K

更新日期：2008-10-01 00:00:00

abstract：:The outer membrane porin OmpF from Escherichia coli has been reconstituted into lipid bilayers of defined composition, and fluorescence spectroscopy is used to characterize its interaction with the surrounding lipid. OmpF is a trimer within the membrane. It contains two Trp residues per monomer, Trp(214) at the lipid-...

journal_title：Biophysical journal

authors： O'Keeffe AH,East JM,Lee AG

更新日期：2000-10-01 00:00:00

abstract：:Many studies of specific protein-nucleic acid binding use short oligonucleotides or restriction fragments, in part to minimize the potential for nonspecific binding of the protein. However, when the specificity ratio is low, multiple nonspecifically bound proteins may occupy the region of DNA corresponding to one spec...

journal_title：Biophysical journal

authors： Tsodikov OV,Holbrook JA,Shkel IA,Record MT Jr

更新日期：2001-10-01 00:00:00

abstract：:The structure of polymer-decorated phospholipid monolayers at the solid-solution interface was investigated using neutron reflectometry. The monolayers were composed of distearoylphosphatidylethanolamine (DSPE) matrixed with varying amounts of DSPE-PEG (DSPE with polyethylene glycol covalently grafted to its headgroup...

journal_title：Biophysical journal

authors： Kuhl TL,Majewski J,Wong JY,Steinberg S,Leckband DE,Israelachvili JN,Smith GS

更新日期：1998-11-01 00:00:00

abstract：:We present a new method for analyzing the dynamics of conformational fluctuations of individual flexible polymer molecules. In single-particle tracking (SPT), one end of the polymer molecule is tethered to an immobile substratum. A microsphere attached to the other end serves as an optical marker. The conformational f...

journal_title：Biophysical journal

authors： Qian H,Elson EL

更新日期：1999-03-01 00:00:00

abstract：:The conformation of single laminin molecules adsorbed on synthetic substrates is directly observed making use of the phase magnitude in tapping mode atomic force microscopy (AFM). With AFM, it is not possible to differentiate the proteins on the substrate if use is made of the height signal, since the roughness of the...

journal_title：Biophysical journal

authors： Rodríguez Hernández JC,Salmerón Sánchez M,Soria JM,Gómez Ribelles JL,Monleón Pradas M

更新日期：2007-07-01 00:00:00

abstract：:The capability to image single microbial cell surfaces at nanometer scale under native conditions would profoundly impact mechanistic and structural studies of pathogenesis, immunobiology, environmental resistance, and biotransformation. Here, using in vitro atomic force microscopy, we have directly visualized high-re...

journal_title：Biophysical journal

authors： Plomp M,Leighton TJ,Wheeler KE,Malkin AJ

更新日期：2005-01-01 00:00:00

abstract：:Interactions of membrane anchored molecules such as glycolipids with a membrane surface are important in determining headgroup conformation. It is therefore essential to represent these membrane surface interactions in molecular modeling studies of glycolipids and other membrane bound molecules. We introduce here an e...

journal_title：Biophysical journal

authors： Ram P,Kim E,Thomson DS,Howard KP,Prestegard JH

更新日期：1992-12-01 00:00:00

abstract：:The brain Na+ channel-1 (BNC1, also known as MDEG1 or ASIC2) is a member of the DEG/ENaC cation channel family. Mutation of a specific residue (Gly430) that lies N-terminal to the second membrane-spanning domain activates BNC1 and converts it from a Na+-selective channel to one permeable to both Na+ and K+. Because al...

journal_title：Biophysical journal

authors： Adams CM,Price MP,Snyder PM,Welsh MJ

更新日期：1999-03-01 00:00:00

abstract：:The interaction between actin and myosin in the filament array of glycerinated muscle fibers has been monitored using paramagnetic probes and mechanical measurements. Both fiber stiffness and the spectra of probes bound to a reactive sulfydral on the myosin head were measured as the actomyosin bond was weakened by add...

journal_title：Biophysical journal

authors： Pate E,Cooke R

更新日期：1988-04-01 00:00:00

abstract：:Ion channels assist and control the diffusion of ions through biological membranes. The conduction process depends on the structural characteristics of these nanopores, among which are the hydrophobicity and the afforded diameter of the conduction pathway. In this contribution, we use full atomistic free-energy molecu...

journal_title：Biophysical journal

authors： Treptow W,Tarek M

更新日期：2006-08-01 00:00:00

abstract：:Cysteine residues introduced by site-directed mutagenesis have been used to probe the conformation and dynamics of two receptors in the E. coli chemotaxis pathway. (a) Thermal motions of the polypeptide backbone were investigated in the periplasmic D-galactose and D-glucose receptor, a globular protein of known struct...

journal_title：Biophysical journal

authors： Careaga CL,Falke JJ

更新日期：1992-04-01 00:00:00

abstract：:Globotriaosylceramide (Gb3), a glycosphingolipid found in the plasma membrane of animal cells, is the endocytic receptor of the bacterial Shiga toxin. Using x-ray reflectivity (XR) and grazing incidence x-ray diffraction (GIXD), lipid monolayers containing Gb3 were investigated at the air-water interface. XR probed Gb...

journal_title：Biophysical journal

authors： Watkins EB,Gao H,Dennison AJC,Chopin N,Struth B,Arnold T,Florent JC,Johannes L

更新日期：2014-09-02 00:00:00

abstract：:High resolution dynamics and structural information has been resolved from 2H solid-state NMR spectra of the Val-1 side-chain of the gramicidin channel in a lipid bilayer. Both powder pattern lineshapes and spectra from uniformly aligned samples of gramicidin in lipid bilayers have been analyzed to achieve a fully con...

journal_title：Biophysical journal

authors： Lee KC,Cross TA

更新日期：1994-05-01 00:00:00

abstract：:The analysis of single-molecule fluorescence resonance energy transfer (FRET) trajectories has become one of significant biophysical interest. In deducing the transition rates between various states of a system for time-binned data, researchers have relied on simple, but often arbitrary methods of extracting rates fro...

journal_title：Biophysical journal

authors： McKinney SA,Joo C,Ha T

更新日期：2006-09-01 00:00:00

abstract：:The electrical membrane potential (Vm) is one of the components of the electrochemical potential of protons across the biological membrane (proton motive force), which powers many vital cellular processes. Because Vm also plays a role in signal transduction, measuring it is of great interest. Over the years, a variety...

journal_title：Biophysical journal

authors： Mancini L,Terradot G,Tian T,Pu Y,Li Y,Lo CJ,Bai F,Pilizota T

更新日期：2020-01-07 00:00:00

abstract：:The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat and ventricular atrial myocytes. The resolution of the ...

journal_title：Biophysical journal

authors： Chen-Izu Y,McCulle SL,Ward CW,Soeller C,Allen BM,Rabang C,Cannell MB,Balke CW,Izu LT

更新日期：2006-07-01 00:00:00

abstract：:Reversible blockage of the voltage-dependent anion channel (VDAC) of the mitochondrial outer membrane by dimeric tubulin is being recognized as a potent regulator of mitochondrial respiration. The tubulin-blocked state of VDAC is impermeant for ATP but only partially closed for small ions. This residual conductance al...

journal_title：Biophysical journal

authors： Gurnev PA,Queralt-Martin M,Aguilella VM,Rostovtseva TK,Bezrukov SM

更新日期：2012-05-02 00:00:00