The high-resolution architecture and structural dynamics of Bacillus spores.

abstract：:Regulation of the F-actin severing activity of gelsolin by Ca2+ has been investigated under physiologic ionic conditions. Tryptophan fluorescence intensity measurements indicate that gelsolin contains at least two Ca2+ binding sites with affinities of 2.5 x 10(7) M-1 and 1.5 x 10(5) M-1. At F-actin and gelsolin concen...

journal_title：Biophysical journal

authors： Kinosian HJ,Newman J,Lincoln B,Selden LA,Gershman LC,Estes JE

更新日期：1998-12-01 00:00:00

abstract：:In the first issue, on the first page of the Biophysical Journal in 1960, Cole and Moore provided the first confirmation of the Hodgkin and Huxley formulation of the sodium and potassium conductances that underlie the action potential. In addition, working with the squid giant axon, Cole and Moore noted that strong hy...

journal_title：Biophysical journal

authors： Hoshi T,Armstrong CM

更新日期：2015-10-06 00:00:00

abstract：:The single photon response in vertebrate phototransduction is highly reproducible despite a number of random components of the activation cascade, including the random activation site, the random walk of an activated receptor, and its quenching in a random number of steps. Here we use a previously generated and tested...

journal_title：Biophysical journal

authors： Bisegna P,Caruso G,Andreucci D,Shen L,Gurevich VV,Hamm HE,DiBenedetto E

更新日期：2008-05-01 00:00:00

abstract：:Numerous isotypes of the structural protein tubulin have now been characterized in various organisms and their expression offers a plausible explanation for observed differences affecting microtubule function in vivo. While this is an attractive hypothesis, there are only a handful of studies demonstrating a direct in...

journal_title：Biophysical journal

authors： Rezania V,Azarenko O,Jordan MA,Bolterauer H,Ludueña RF,Huzil JT,Tuszynski JA

更新日期：2008-08-01 00:00:00

abstract：:By using an analogy between the magnetization of a paramagnetic material in an external magnetic field and the electric polarization of the lateral wall of outer hair cells in response to the transmembrane potential, we show that, based on experimental data on the charge transfer across the membrane, it is impossible ...

journal_title：Biophysical journal

authors： Scherer MP,Gummer AW

更新日期：2005-05-01 00:00:00

abstract：:We perform single-molecule flow experiments using confocal microscopy and a microfluidic device for shear rates up to 20,000 s-1 and present results for the shear-induced unraveling and elongation of tethered von Willebrand factor (VWF) multimers. Further, we employ companion Brownian dynamics simulations to help expl...

journal_title：Biophysical journal

authors： Wang Y,Morabito M,Zhang XF,Webb E 3rd,Oztekin A,Cheng X

更新日期：2019-06-04 00:00:00

abstract：:2D-ELDOR spectroscopy has been employed to study the dynamic structure of the liquid-ordered (Lo) phase versus that of the liquid-crystalline (Lc) phase in multibilayer phospholipid vesicles without (Lc) and with (Lo) cholesterol, using end-chain and headgroup labels and spin-labeled cholestane. The spectra are in mos...

journal_title：Biophysical journal

authors： Costa-Filho AJ,Shimoyama Y,Freed JH

更新日期：2003-04-01 00:00:00

abstract：:It has long been appreciated that spatiotemporal dynamics of cell migration are under the control of intracellular signaling pathways, which are mediated by adhesion receptors and other transducers of extracellular cues. Further, there is ample evidence that aspects of cell migration are stochastic: how else could it ...

journal_title：Biophysical journal

authors： Weiger MC,Ahmed S,Welf ES,Haugh JM

更新日期：2010-01-06 00:00:00

abstract：:The effects of cholesterol (Chol) on phospholipid bilayers include ordering of the fatty acyl chains, condensing of the lipids in the bilayer plane, and promotion of the liquid-ordered phase. These effects depend on the type of phospholipids in the bilayer and are determined by the nature of the underlying molecular i...

journal_title：Biophysical journal

authors： Aittoniemi J,Niemelä PS,Hyvönen MT,Karttunen M,Vattulainen I

更新日期：2007-02-15 00:00:00

abstract：:Purified cytochrome c oxidase CO complex from beef heart has been studied by Fourier transform infrared absorbance difference spectroscopy. Photolysis at 10-20 Kelvin results in dissociation of a3FeCO, formation of CuBCO, and perturbation of the a3-heme and CuB complex. The vibrational perturbation spectrum between 90...

journal_title：Biophysical journal

authors： Park S,Pan LP,Chan SI,Alben JO

更新日期：1996-08-01 00:00:00

abstract：:Flow dialysis has found widespread use in determining the dissociation constant (KD) of a protein-ligand interaction or the amount of available binding sites (E0). This method has the potency to measure both these parameters in a single experiment and in this article a method to measure simultaneously the KD and E0 is...

journal_title：Biophysical journal

authors： Veldhuis G,Vos EP,Broos J,Poolman B,Scheek RM

更新日期：2004-04-01 00:00:00

abstract：:The interactions between phospholipid molecules in suspensions have been studied by using mass spectrometry. Electrospray mass spectra of homogeneous preparations formed from three different phospholipid molecules demonstrate that under certain conditions interactions between 90 and 100 lipid molecules can be preserve...

journal_title：Biophysical journal

authors： Hanson CL,Ilag LL,Malo J,Hatters DM,Howlett GJ,Robinson CV

更新日期：2003-12-01 00:00:00

abstract：:We present a molecular system where polymerization is controlled externally by tuning the elastic energy of the monomers. The elastic energy, provided by a DNA molecular spring, destabilizes the monomer state through a process analogous to domain swapping. This energy can be large (of approximately 10 kT) and thus dri...

journal_title：Biophysical journal

authors： Wang A,Zocchi G

更新日期：2009-03-18 00:00:00

abstract：:Macromolecular transport between the nucleus and cytoplasm occurs through the nuclear pore complexes (NPCs). The NPC in the budding yeast Saccharomyces cerevisiae is a 60-MDa structure embedded in the nuclear envelope and composed of ~30 proteins, termed nucleoporins or nups. Here we present a large-scale analysis of ...

journal_title：Biophysical journal

authors： Damelin M,Silver PA

更新日期：2002-12-01 00:00:00

abstract：:The voltage-gated potassium channel protein KvLQT1 (Wang et al., 1996. Nature Genet. 12:17-23) is believed to underlie the delayed rectifier potassium current of cardiac muscle together with the small membrane protein minK (also named IsK) as an essential auxiliary subunit (Barhanin et al., 1996. Nature. 384:78-80; Sa...

journal_title：Biophysical journal

authors： Pusch M,Magrassi R,Wollnik B,Conti F

更新日期：1998-08-01 00:00:00

abstract：:For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating ...

journal_title：Biophysical journal

authors： Hustedt EJ,Smirnov AI,Laub CF,Cobb CE,Beth AH

更新日期：1997-04-01 00:00:00

abstract：:The solution structures of the N-terminal domains of protein S, a plasma vitamin K-dependent glycoprotein, and its homolog growth arrest specific protein 6 (Gas6) were predicted by molecular dynamics computer simulations. The initial structures were based on the x-ray crystallographic structure of the corresponding re...

journal_title：Biophysical journal

authors： Perera L,Li L,Darden T,Monroe DM,Pedersen LG

更新日期：1997-10-01 00:00:00

abstract：:A two-dimensional stochastic model for the dynamics of microtubules in gliding-assay experiments is presented here, which includes the viscous drag acting on the moving fiber and the interaction with the kinesins. For this purpose, we model kinesin as a spring, and explicitly use parameter values to characterize the m...

journal_title：Biophysical journal

authors： Gibbons F,Chauwin JF,Despósito M,José JV

更新日期：2001-06-01 00:00:00

abstract：:Nuclear calcium is a key signal in the dialogue between synapse and nucleus that controls the genomic responses required for persistent adaptations, including memory and acquired neuroprotection. The amplitude and duration of nuclear calcium transients specify activity-induced transcriptional changes. However, the pre...

journal_title：Biophysical journal

authors： Bengtson CP,Freitag HE,Weislogel JM,Bading H

更新日期：2010-12-15 00:00:00

abstract：:Enzyme function depends on specific conformational motions. We show that the temperature dependence of enzyme kinetic parameters can provide insight into these functionally relevant motions. While investigating the catalytic properties of IPMDH from Escherichia coli, we found that its catalytic efficiency (k(cat)/K(M,...

journal_title：Biophysical journal

authors： Hajdú I,Szilágyi A,Kardos J,Závodszky P

更新日期：2009-06-17 00:00:00

abstract：:As double-stranded DNA is stretched to its B-form contour length, models of polymer elasticity can describe the dramatic increase in measured force. When the molecule is stretched beyond this contour length, it shows a highly cooperative overstretching transition. We have measured the elasticity and overstretching tra...

journal_title：Biophysical journal

authors： Wenner JR,Williams MC,Rouzina I,Bloomfield VA

更新日期：2002-06-01 00:00:00

abstract：:The enzyme F1-adenosine triphosphatase (ATPase) is a molecular motor that converts the chemical energy stored in the molecule adenosine triphosphate (ATP) into mechanical rotation of its gamma-subunit. During steady-state catalysis, the three catalytic sites of F1 operate in a cooperative fashion such that at every in...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2004-11-01 00:00:00

abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

journal_title：Biophysical journal

authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

更新日期：2010-10-20 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:The four-way DNA (Holliday) junction is an important postulated intermediate in the process of genetic recombination. Earlier studies have suggested that the junction exists in two alternative conformations, depending upon the salt concentration present. At high salt concentrations the junction folds into a stacked X ...

journal_title：Biophysical journal

authors： Clegg RM,Murchie AI,Lilley DM

更新日期：1994-01-01 00:00:00

abstract：:Topical microbicide products are being developed for the prevention of sexually transmitted infections. These include vaginally-applied gels that deliver anti-HIV molecules. Gels may also provide partial barriers that slow virion diffusion from semen to vulnerable epithelium, increasing the time during which anti-HIV ...

journal_title：Biophysical journal

authors： Lai BE,Henderson MH,Peters JJ,Walmer DK,Katz DF

更新日期：2009-11-04 00:00:00

abstract：:Using the substituted-cysteine-accessibility method, we previously showed that a cysteine residue introduced to the Y512 position of CLC-0 was more rapidly modified by a negatively charged methanethiosulfonate (MTS) reagent, 2-sulfonatoethyl MTS (MTSES), than by the positively charged 2-(trimethylammonium)ethyl MTS (M...

journal_title：Biophysical journal

authors： Zhang XD,Yu WP,Chen TY

更新日期：2010-02-03 00:00:00

abstract：:Cell adhesion provides not only physical linkage but also communication between the cell and its environment. As such, it is important to many cellular functions. Recently, the probability distribution of forming a low number of specific adhesive bonds in a short-duration contact has been described (Chesla et al., Bio...

journal_title：Biophysical journal

authors： Zhu C,Williams TE

更新日期：2000-10-01 00:00:00

abstract：:We have studied the low-resolution solution conformation of a Holliday (or four-way) DNA junction by using small-angle x-ray scattering, sedimentation velocity, and computational modeling techniques. The scattering data were analyzed in two independent ways: firstly, by rigid-body modeling of the scattering data using...

journal_title：Biophysical journal

authors： Nöllmann M,Stark WM,Byron O

更新日期：2004-05-01 00:00:00

abstract：:Dendritic shaft (Zd) and spine (Zsp) input impedances were computed numerically for sites on hippocampal neurons, using a segmental format of cable calculations. The Zsp values for a typical spine appended onto a dendritic shaft averaged less than 2% higher than the Zd values for the adjacent dendritic shaft. Spine sy...

journal_title：Biophysical journal

authors： Turner DA

更新日期：1984-07-01 00:00:00