Insight into the putative specific interactions between cholesterol, sphingomyelin, and palmitoyl-oleoyl phosphatidylcholine.

abstract：:Hepatitis C is a major worldwide disease and health hazard, affecting ∼3% of the world population. The p7 protein of hepatitis C virus (HCV) is an intracellular ion channel and pH regulator that is involved in the viral replication cycle. It is targeted by various classical ion channel blockers. Here, we generated p7 ...

journal_title：Biophysical journal

authors： Breitinger U,Farag NS,Ali NKM,Breitinger HA

更新日期：2016-06-07 00:00:00

abstract：:Cochlear outer hair cells undergo reversible changes in shape when externally stimulated. This response, known as OHC motility, is a central component of the cochlear amplifier, the mechanism responsible for the high sensitivity of mammalian hearing. We report that actin depolymerization, as regulated by activation/in...

journal_title：Biophysical journal

authors： Matsumoto N,Kitani R,Maricle A,Mueller M,Kalinec F

更新日期：2010-10-06 00:00:00

abstract：:Globotriaosylceramide (Gb3), a glycosphingolipid found in the plasma membrane of animal cells, is the endocytic receptor of the bacterial Shiga toxin. Using x-ray reflectivity (XR) and grazing incidence x-ray diffraction (GIXD), lipid monolayers containing Gb3 were investigated at the air-water interface. XR probed Gb...

journal_title：Biophysical journal

authors： Watkins EB,Gao H,Dennison AJC,Chopin N,Struth B,Arnold T,Florent JC,Johannes L

更新日期：2014-09-02 00:00:00

abstract：:We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force...

journal_title：Biophysical journal

authors： Ounkomol C,Yamada S,Heinrich V

更新日期：2010-12-15 00:00:00

abstract：:Single-stranded junctions/loops are frequently occurring structural motifs in nucleic acid structures. Due to the polyanionic nature of the nucleic acid backbone, metal ions play a crucial role in the loop stability. Here we use the tightly bound ion theory, which can account for the possible ion correlation and ensem...

journal_title：Biophysical journal

authors： Tan ZJ,Chen SJ

更新日期：2008-07-01 00:00:00

abstract：:In Shaker-like channels, the activation gate is formed at the bundle crossing by the convergence of the inner S6 helices near a conserved proline-valine-proline motif, which introduces a kink that allows for electromechanical coupling with voltage sensor motions via the S4-S5 linker. Human ether-a-go-go-related gene (...

journal_title：Biophysical journal

authors： Thouta S,Sokolov S,Abe Y,Clark SJ,Cheng YM,Claydon TW

更新日期：2014-03-04 00:00:00

abstract：:Photosystem II catalyzes photosynthetic water oxidation in plants, green algae, and cyanobacteria. The manganese-containing active site cycles through a series of five oxidation states, S(n), where n refers to the number of oxidizing equivalents stored. In this report, reaction-induced Fourier transform infrared and e...

journal_title：Biophysical journal

authors： Halverson KM,Barry BA

更新日期：2003-10-01 00:00:00

abstract：:We have formulated a kinetic model for the primary steps that occur at the cell membrane during receptor-mediated endocytosis. This model includes the diffusion of receptor molecules, the binding of receptors to coated pits, the loss of coated pits by invagination, and random reinsertion of receptors and coated pits. ...

journal_title：Biophysical journal

authors： Keizer J,Ramirez J,Peacock-López E

更新日期：1985-01-01 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:Nipah is an emerging paramyxovirus that is of serious concern to human health. It invades host cells using two of its membrane proteins-G and F. G binds to host ephrins and this stimulates G to activate F. Upon activation, F mediates virus-host membrane fusion. Here we focus on mechanisms that underlie the stimulation...

journal_title：Biophysical journal

authors： Dutta P,Siddiqui A,Botlani M,Varma S

更新日期：2016-10-18 00:00:00

abstract：:We have studied the photoinduced volume changes, energetics, and kinetics in the early steps of the bacteriorhodopsin (BR) photocycle with pulsed, time-resolved photoacoustics. Our data show that there are two volume changes. The fast volume change ( < or = 200 ns) is an expansion (2.5 +/- 0.3 A3/molecule) and is obse...

journal_title：Biophysical journal

authors： Zhang D,Mauzerall D

更新日期：1996-07-01 00:00:00

abstract：:Batrachotoxin (BTX) alters the gating of voltage-gated Na(+) channels and causes these channels to open persistently, whereas local anesthetics (LAs) block Na(+) conductance. The BTX and LA receptors have been mapped to several common residues in D1-S6 and D4-S6 segments of the Na(+) channel alpha-subunit. We substitu...

journal_title：Biophysical journal

authors： Wang SY,Nau C,Wang GK

更新日期：2000-09-01 00:00:00

abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

journal_title：Biophysical journal

authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

更新日期：2010-07-21 00:00:00

abstract：:The demembranated (skinned) muscle fiber preparation is widely used to investigate muscle contraction because the intracellular ionic conditions can be precisely controlled. However, plasma membrane removal results in a loss of osmotic regulation, causing abnormal hydration of the myofilament lattice and its proteins....

journal_title：Biophysical journal

authors： Tanner BC,Farman GP,Irving TC,Maughan DW,Palmer BM,Miller MS

更新日期：2012-09-19 00:00:00

abstract：:Binding features of heroin in whole blood and in blood fractions were delineated by measuring the selective spin-lattice relaxation rates of heroin protons in physiologic conditions. Interaction with some receptor located in the whole human blood or in the human plasma was detected and the apparent binding constant ca...

journal_title：Biophysical journal

authors： Barni Comparini I,Gaggelli E,Marchettini N,Valensin G

更新日期：1985-08-01 00:00:00

abstract：:Wiener kernels have been measured for the light growth response of a number of mutants of Phycomyces which show abnormal phototropism (mad mutants). Representative mutants were chosen from the six complementation groups (madA to madF) associated with the light response pathway. One group, madA, associated with the inp...

journal_title：Biophysical journal

authors： Lipson ED

更新日期：1975-10-01 00:00:00

abstract：:Motions through the energy landscape of proteins lead to biological function. At temperatures below a dynamical transition (150-250 K), some of these motions are arrested and the activity of some proteins ceases. Here, we introduce the technique of temperature-derivative fluorescence microspectrophotometry to investig...

journal_title：Biophysical journal

authors： Weik M,Vernede X,Royant A,Bourgeois D

更新日期：2004-05-01 00:00:00

abstract：:Acyl chain dynamics of the trans-unsaturated lipid, dielaidoylphosphatidylcholine (DEPC), were studied by conventional and saturation transfer electron paramagnetic resonance spectroscopy of aqueous dispersions of DEPC spin labeled with lecithins having doxyl groups at positions 5, 10, and 14 on the sn-2 chain. The ge...

journal_title：Biophysical journal

authors： Dalton LA,Miller KW

更新日期：1993-10-01 00:00:00

abstract：:The excitation of the squid giant axon that had been perfused intra- and extracellularly with solutions containing a high concentration of glycerol could be observed below 0 degree C. The action potential could be elicited at normal strengths of electrical stimuli. The time-course of the action potential was slowed, w...

journal_title：Biophysical journal

authors： Kukita F,Yamagishi S

更新日期：1981-07-01 00:00:00

abstract：:During inflammation, flowing leukocytes tether to and roll on vascular surfaces through the association and dissociation of selectin/ligand bonds. The interactions of P- and L- selectins with their respective ligands exhibit catch-slip bonds, such that increasing force initially prolongs and then shortens bond lifetim...

journal_title：Biophysical journal

authors： Wayman AM,Chen W,McEver RP,Zhu C

更新日期：2010-08-09 00:00:00

abstract：:The genetic information in DNA is transcribed to mRNA and then translated to proteins, which form the building blocks of life. Translation, or protein synthesis, is hence a central cellular process. We have developed a gene-sequence-specific mechanistic model for the translation machinery, which accounts for all the e...

journal_title：Biophysical journal

authors： Zouridis H,Hatzimanikatis V

更新日期：2007-02-01 00:00:00

abstract：:The ryanodine receptor (RyR) of skeletal muscle contains two functional domains: a carboxyl-terminal hydrophobic domain that forms the putative conduction pore of the calcium release channel, and a large cytoplasmic domain that corresponds to the "foot structure." To understand the contribution of the foot structure t...

journal_title：Biophysical journal

authors： Bhat MB,Zhao J,Hayek S,Freeman EC,Takeshima H,Ma J

更新日期：1997-09-01 00:00:00

abstract：:The cell membrane of Salinibacter ruber contains xanthorhodopsin, a light-driven transmembrane proton pump with two chromophores: a retinal and the carotenoid, salinixanthin. Action spectra for transport had indicated that light absorbed by either is utilized for function. If the carotenoid is an antenna in this prote...

journal_title：Biophysical journal

authors： Balashov SP,Imasheva ES,Wang JM,Lanyi JK

更新日期：2008-09-01 00:00:00

abstract：:Transcription factor (TF) proteins rapidly locate unique target sites on long genomic DNA molecules--and bind to them--during gene regulation. The search mechanism is known to involve a combination of three-dimensional diffusion through the bulk of the cell and one-dimensional sliding diffusion along the DNA. It is be...

journal_title：Biophysical journal

authors： Hu L,Grosberg AY,Bruinsma R

更新日期：2008-08-01 00:00:00

abstract：:Despite the importance of lipid rafts, commonly defined as liquid-ordered domains rich in cholesterol and in lipids with high gel-to-fluid melting temperatures (T(m)), the rules for raft formation in membranes are not completely understood. Here, a fluorescence-quenching strategy was used to define how lipids with low...

journal_title：Biophysical journal

authors： Bakht O,Pathak P,London E

更新日期：2007-12-15 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:Bacterial endotoxins (lipopolysaccharides (LPS)) are strong elicitors of the human immune system by interacting with serum and membrane proteins such as lipopolysaccharide-binding protein (LBP) and CD14 with high specificity. At LPS concentrations as low as 0.3 ng/ml, such interactions may lead to severe pathophysiolo...

journal_title：Biophysical journal

authors： Kaconis Y,Kowalski I,Howe J,Brauser A,Richter W,Razquin-Olazarán I,Iñigo-Pestaña M,Garidel P,Rössle M,Martinez de Tejada G,Gutsmann T,Brandenburg K

更新日期：2011-06-08 00:00:00

abstract：:Historically, predicting macroscopic blood flow characteristics such as viscosity has been an empirical process due to the difficulty in rigorously including the particulate nature of blood in a mathematical representation of blood rheology. Using a two-dimensional lattice Boltzmann approach, we have simulated the flo...

journal_title：Biophysical journal

authors： Sun C,Munn LL

更新日期：2005-03-01 00:00:00

abstract：:The protonation state and intracellular distribution of ellipticine were investigated in single human mammary T47D cells by confocal laser microspectrofluorimetry. In the cell nucleus, only the protonated form of ellipticine was detected as a direct consequence of its apparent pK increase upon DNA binding. Both proton...

journal_title：Biophysical journal

authors： Sureau F,Moreau F,Millot JM,Manfait M,Allard B,Aubard J,Schwaller MA

更新日期：1993-11-01 00:00:00

abstract：:Inactivation is a fundamental property of voltage-gated ion channels. Fast inactivation of Na(+) channels involves channel block by the III-IV cytoplasmic interdomain linker. The mechanisms of nonfast types of inactivation (intermediate, slow, and ultraslow) are unclear, although the ionic environment and P-loops rear...

journal_title：Biophysical journal

authors： Tikhonov DB,Zhorov BS

更新日期：2007-09-01 00:00:00