Microspectrofluorometry of the protonation state of ellipticine, an antitumor alkaloid, in single cells.

abstract：:F-actin, a helical polymer formed by polymerization of the monomers (G-actin), plays crucial roles in various aspects of cell motility. Flexibility of F-actin has been suggested to be important for such a variety of functions. Understanding the flexibility of F-actin requires characterization of a hierarchy of dynamic...

journal_title：Biophysical journal

authors： Fujiwara S,Plazanet M,Matsumoto F,Oda T

更新日期：2008-06-01 00:00:00

abstract：:The theoretical basis of an optical microscope technique to image dynamically scattered light fluctuation decay rates (dynamic light scattering microscopy) is developed. It is shown that relative motions between scattering centers even smaller than the optical resolution of the microscope are sufficient to produce sig...

journal_title：Biophysical journal

authors： Dzakpasu R,Axelrod D

更新日期：2004-08-01 00:00:00

abstract：:The energy transfer processes between carotenoids and Chls have been studied by femtosecond transient absorption in the CP29-WT complex, which contains only two carotenoids per polypeptide located in the L1 and L2 sites, and in the CP29-E166V mutant in which only the L1 site is occupied. The comparison of these two sa...

journal_title：Biophysical journal

authors： Croce R,Müller MG,Caffarri S,Bassi R,Holzwarth AR

更新日期：2003-04-01 00:00:00

abstract：:In high light conditions, cyanobacteria dissipate excess absorbed energy as heat in the light-harvesting phycobilisomes (PBs) to protect the photosynthetic system against photodamage. This process requires the binding of the red active form of the Orange Carotenoid Protein (OCP(r)), which can effectively quench the ex...

journal_title：Biophysical journal

authors： Tian L,Gwizdala M,van Stokkum IH,Koehorst RB,Kirilovsky D,van Amerongen H

更新日期：2012-04-04 00:00:00

abstract：:Synchrotron radiation circular dichroism, Fourier transform infrared, and nuclear magnetic resonance spectroscopies, and small-angle x-ray scattering were used to monitor the reversible thermal unfolding of hen egg white lysozyme. The results were compared with crystal structures and high- and low-temperature structur...

journal_title：Biophysical journal

authors： Meersman F,Atilgan C,Miles AJ,Bader R,Shang W,Matagne A,Wallace BA,Koch MH

更新日期：2010-10-06 00:00:00

abstract：:Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are attributed to envir...

journal_title：Biophysical journal

authors： Tata DB,Foresti M,Cordero J,Tomashefsky P,Alfano MA,Alfano RR

更新日期：1986-09-01 00:00:00

abstract：:The stability of OmpA in large unilamellar vesicles of dilauroyl phosphatidylcholine was studied using different concentrations of urea. The effective energy of unfolding, as determined from refolding experiments, is greater than that for small sonicated unilamellar vesicles by an amount that is compatible with estima...

journal_title：Biophysical journal

authors： Pocanschi CL,Patel GJ,Marsh D,Kleinschmidt JH

更新日期：2006-10-15 00:00:00

abstract：:Molecular dynamics calculations demonstrated the conformational change in the prion protein due to Ala(117)-->Val mutation, which is related to Gerstmann-Sträussler-Sheinker disease, one of the familial prion diseases. Three kinds of model structures of human and mouse prion proteins were examined: (model 1) nuclear m...

journal_title：Biophysical journal

authors： Okimoto N,Yamanaka K,Suenaga A,Hata M,Hoshino T

更新日期：2002-05-01 00:00:00

abstract：:Membranes of the lens of the eye of mammals have two particular characteristics, high concentrations of sphingomyelin, and dihydrosphingomyelin and cholesterol. We have studied the miscibility of cholesterol with both egg sphingomyelin and with dihydrosphingomyelin made by hydrogenation of egg sphingomyelin. At a chol...

journal_title：Biophysical journal

authors： Epand RM

更新日期：2003-05-01 00:00:00

abstract：:Thermodynamic analysis of metabolic networks has recently generated increasing interest for its ability to add constraints on metabolic network operation, and to combine metabolic fluxes and metabolite measurements in a mechanistic manner. Concepts for the calculation of the change in Gibbs energy of biochemical react...

journal_title：Biophysical journal

authors： Jol SJ,Kümmel A,Hatzimanikatis V,Beard DA,Heinemann M

更新日期：2010-11-17 00:00:00

abstract：:In voltage-clamped frog skeletal muscle fibers, Ca(2+) release rises rapidly to a peak, then decays to a nearly steady state. The voltage dependence of the ratio of amplitudes of these two phases (p/s) shows a maximum at low voltages and declines with further depolarization. The peak phase has been attributed to a com...

journal_title：Biophysical journal

authors： Brum G,Piriz N,DeArmas R,Rios E,Stern M,Pizarro G

更新日期：2003-07-01 00:00:00

abstract：:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Prot...

journal_title：Biophysical journal

authors： van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

更新日期：2013-03-05 00:00:00

abstract：:During postnatal development, major changes in mechanical properties of skeletal muscle occur. We investigated passive properties of skeletal muscle in mice and rabbits that varied in age from 1 day to approximately 1 year. Neonatal skeletal muscle expressed large titin isoforms directly after birth, followed by a gra...

journal_title：Biophysical journal

authors： Ottenheijm CA,Knottnerus AM,Buck D,Luo X,Greer K,Hoying A,Labeit S,Granzier H

更新日期：2009-10-21 00:00:00

abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

journal_title：Biophysical journal

authors： Lee AI,Brody JP

更新日期：2005-06-01 00:00:00

abstract：:Proton translocation activity of bacteriorhodopsin mutants lacking the proton acceptor Asp-85 was investigated using the black lipid membrane technique. Mutants D85N, D85T, and D85,96N were constructed and homologously expressed in Halobacterium salinarium to yield a membrane fraction with a buoyant density of 1.18 g/...

journal_title：Biophysical journal

authors： Tittor J,Schweiger U,Oesterhelt D,Bamberg E

更新日期：1994-10-01 00:00:00

abstract：:The hyperpolarization-activated cyclic nucleotide-modulated (HCN) cation channels are opened by membrane hyperpolarization, while their activation is modulated by the binding of cyclic adenosine monophosphate (cAMP) in the cytoplasm. Here we investigate the molecular basis of cAMP channel modulation by performing mole...

journal_title：Biophysical journal

authors： Berrera M,Pantano S,Carloni P

更新日期：2006-05-15 00:00:00

abstract：:We have studied the combined effects of X-irradiation and nitrogen mustard treatment on the colony-forming ability of Chinese hamster cells. In contrast to X-irradiation, nitrogen mustard acting by itself yields an exponential dose-effect relationship. Moreover, whether delivered in immediate sequence or after varying...

journal_title：Biophysical journal

authors： Sakamoto K,Elkind MM

更新日期：1969-09-01 00:00:00

abstract：:Thin filament regulation of contraction is thought to involve the binding of two activating ligands: Ca2+ and strongly bound cross-bridges. The specific cross-bridge states required to promote thin filament activation have not been identified. This study examines the relationship between cross-bridge cycling and thin ...

journal_title：Biophysical journal

authors： Wahr PA,Metzger JM

更新日期：1999-04-01 00:00:00

abstract：:The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...

journal_title：Biophysical journal

authors： Lacroix JJ,Bezanilla F

更新日期：2012-08-08 00:00:00

abstract：:To the best of our knowledge, this is the first report on the structure of product-inhibited mammalian peroxidase. Lactoperoxidase is a heme containing an enzyme that catalyzes the inactivation of a wide range of microorganisms. In the presence of hydrogen peroxide, it preferentially converts thiocyanate ion into a to...

journal_title：Biophysical journal

authors： Singh AK,Singh N,Sharma S,Shin K,Takase M,Kaur P,Srinivasan A,Singh TP

更新日期：2009-01-01 00:00:00

abstract：:The distribution of low concentrations of ganglioside GM1 in L-alpha-dipalmitoylphosphatidylcholine (DPPC) and DPPC/cholesterol monolayers supported on mica has been studied using atomic force microscopy (AFM). The monolayers studied correspond to a pure gel phase and a mixture of liquid-expanded (LE) and liquid-conde...

journal_title：Biophysical journal

authors： Yuan C,Johnston LJ

更新日期：2000-11-01 00:00:00

abstract：:The three phases of dipalmitoylphosphatidylcholine-water dispersions, occurring below the main transition are studied by a moment analysis of 1H-nuclear magnetic resonance (NMR) spectra. The subtransition, recently detected by Chen, S. C., J. M. Sturtevant, and B. J. Gaffney, 1980, Pro. Natl. Acad. Sci. USA, 77:5060-5...

journal_title：Biophysical journal

authors： Trahms L,Klabe WD,Boroske E

更新日期：1983-06-01 00:00:00

abstract：:Current methods for fluorescence resonance energy transfer (FRET) microscopy of living cells involve taking a series of images with alternating excitation colors in separate camera exposures. Here we present a new FRET method based on polarization that requires only one camera exposure and thereby offers the possibili...

journal_title：Biophysical journal

authors： Mattheyses AL,Hoppe AD,Axelrod D

更新日期：2004-10-01 00:00:00

abstract：:X-ray edge absorption of copper and extended fine structure studies of both copper and iron centers have been made of cytochrome oxidase from beef heart, Paracoccus dentrificans, and HB-8 thermophilic bacteria (1-2.5 mM in heme). The desired redox state (fully oxidized, reduced CO, mixed valence formate and CO) in the...

journal_title：Biophysical journal

authors： Powers L,Chance B,Ching Y,Angiolillo P

更新日期：1981-06-01 00:00:00

abstract：:Integrin-mediated signaling is crucial for cell-substrate adhesion and can be triggered from both intra- and extracellular interactions. Although talin binding is sufficient for inside-out activation of integrin, other cytoplasmic proteins such as α-actinin and filamin can directly interfere with talin-mediated integr...

journal_title：Biophysical journal

authors： Shams H,Mofrad MRK

更新日期：2017-08-22 00:00:00

abstract：:Two independent methods, induced osmosis and solvent drag, were used to determine the reflection coefficients for NaCl (sigma NaCl) in brush border and basolateral membrane vesicles isolated from rabbit proximal tubule. In the induced osmosis method, vesicles loaded with sucrose were subjected to varying inward NaCl g...

journal_title：Biophysical journal

authors： Pearce D,Verkman AS

更新日期：1989-06-01 00:00:00

abstract：:Beat-to-beat alternation of the action potential duration (APD) in paced cardiac cells has been linked to the onset of lethal arrhythmias. Both experimental and theoretical studies have shown that alternans at the single cell level can be caused by unstable membrane voltage (V(m)) dynamics linked to steep APD-restitut...

journal_title：Biophysical journal

authors： Sato D,Shiferaw Y,Qu Z,Garfinkel A,Weiss JN,Karma A

更新日期：2007-02-15 00:00:00

abstract：:The bonding rules for actin filament bundles do not lead to a particular packing symmetry, but allow for either regular or disordered filament packing. Indeed, both hexagonal and disordered types of packing are observed in vivo. To investigate factors which control bundle order, as well as size, we examined the effect...

journal_title：Biophysical journal

authors： Stokes DL,DeRosier DJ

更新日期：1991-02-01 00:00:00

abstract：:Nisin is an antimicrobial peptide used as food preservative. To gain some insights into the hypothesis that its bactericidal activity is due to the perturbation of the lipid fraction of the bacterial plasmic membrane, we have investigated the effect of nisin on model phosphatidylcholine (PC) membranes. We show that ni...

journal_title：Biophysical journal

authors： El Jastimi R,Edwards K,Lafleur M

更新日期：1999-08-01 00:00:00

abstract：:This paper describes an analysis of microscopic models for the coupling between ion flow and rotation of bacterial flagella. In model I it is assumed that intersecting half-channels exist on the rotor and the stator and that the driving ion is constrained to move together with the intersection site. Model II is based ...

journal_title：Biophysical journal

authors： Läuger P

更新日期：1988-01-01 00:00:00