Temperature derivative fluorescence spectroscopy as a tool to study dynamical changes in protein crystals.

abstract：:The incompressibility of the lipid bilayer keeps the total surface area of the red cell membrane constant. Local conservation of membrane surface area requires that each surface element of the membrane skeleton keeps its area when its aspect ratio is changed. A change in area would require a flow of lipids past the in...

journal_title：Biophysical journal

authors： Fischer TM

更新日期：1992-02-01 00:00:00

abstract：:With a view to determine the configuration and regularity of plectonemically supercoiled DNA, we have measured the small angle neutron scattering from pUC18 plasmid in saline solutions. Furthermore, we have derived the mathematical expression for the single chain scattering function (form factor) of a superhelical str...

journal_title：Biophysical journal

authors： Zakharova SS,Jesse W,Backendorf C,Egelhaaf SU,Lapp A,van der Maarel JR

更新日期：2002-08-01 00:00:00

abstract：:The glucocorticoid receptor (GR) is a steroid-hormone-activated transcription factor that modulates gene expression. Transcriptional regulation by the GR requires dynamic receptor binding to specific target sites located across the genome. This binding remodels the chromatin structure to allow interaction with other t...

journal_title：Biophysical journal

authors： Muratcioglu S,Presman DM,Pooley JR,Grøntved L,Hager GL,Nussinov R,Keskin O,Gursoy A

更新日期：2015-09-15 00:00:00

abstract：:Three features appear to characterize steady-state light adaptation in vertebrate cone photoreceptors: (a) the shape of the "log intensity-response" curve at different levels of adaptation is the same, the only change with adaptation is in the position of the point on the curve about which the cones operate; (b) at hi...

journal_title：Biophysical journal

authors： Dawis SM,Purple RL

更新日期：1982-08-01 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:A complete description of protein structure and function must include a proper treatment of mechanisms that lead to cooperativity. The helix/coil transition serves as a simple example of a cooperative folding process, commonly described by a nucleation-propagation mechanism. The prevalent view is that coil structure m...

journal_title：Biophysical journal

authors： Vorov OK,Livesay DR,Jacobs DJ

更新日期：2009-12-02 00:00:00

abstract：:Although significant progress has been made toward understanding morphogen-mediated patterning in development, control of the size and shape of tissues via local and global signaling is poorly understood. In particular, little is known about how cell-cell interactions are involved in the control of tissue size. The Hi...

journal_title：Biophysical journal

authors： Gou J,Lin L,Othmer HG

更新日期：2018-08-21 00:00:00

abstract：:The active amplification of sound-induced vibrations in the cochlea, known to be crucial for auditory sensitivity and frequency selectivity, is not well understood. The outer hair cell (OHC) somatic electromotility is a potential mechanism for such amplification. Its effectiveness in vivo is putatively limited by the ...

journal_title：Biophysical journal

authors： Ramamoorthy S,Nuttall AL

更新日期：2012-02-08 00:00:00

abstract：:Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the la...

journal_title：Biophysical journal

authors： Vulin C,Di Meglio JM,Lindner AB,Daerr A,Murray A,Hersen P

更新日期：2014-05-20 00:00:00

abstract：:Hydrophobic interactions between a bilayer and its embedded membrane proteins couple protein conformational changes to changes in the packing of the surrounding lipids. The energetic cost of a protein conformational change therefore includes a contribution from the associated bilayer deformation energy (DeltaGdef0), w...

journal_title：Biophysical journal

authors： Lundbaek JA,Andersen OS

更新日期：1999-02-01 00:00:00

abstract：:Phase-resolved monitoring of photoacoustic signals can provide information about the depth profile of a sample. We describe an application of this principle to determine the depth profiles of ligands and antitumor agents in mammalian cells. Measurements of the in-phase and quadrature components of the photoacoustic sp...

journal_title：Biophysical journal

authors： Narayanan K,Chandani S,Ramakrishna T,Rao CM

更新日期：1997-05-01 00:00:00

abstract：:Muscle contraction occurs as the result of actin-myosin interaction, which is mediated by the intermolecular forces exerted at the actin-myosin interface. To obtain information about the nature of these intermolecular forces, we tested the sensitivity of various contractile parameters of skinned skeletal muscle fibers...

journal_title：Biophysical journal

authors： Iwamoto H

更新日期：2000-06-01 00:00:00

abstract：:To investigate the mechanisms by which vesicles of pulmonary surfactant adsorb to an air-liquid interface, we measured the effect of different phospholipids and of their concentration both in the subphase and at the interface on this process. Adsorbing vesicles contained the hydrophobic surfactant proteins mixed with ...

journal_title：Biophysical journal

authors： Walters RW,Jenq RR,Hall SB

更新日期：2000-01-01 00:00:00

abstract：:Bipolar tetraether lipids (BTLs) are abundant in crenarchaeota, which thrive in both thermophilic and nonthermophilic environments, with wide-ranging growth temperatures (4-108°C). BTL liposomes can serve as membrane models to explore the role of BTLs in the thermal stability of the plasma membrane of crenarchaeota. I...

journal_title：Biophysical journal

authors： Chong PL,Sulc M,Winter R

更新日期：2010-11-17 00:00:00

abstract：:In this study we describe the expression and function of the two rat type-1 inositol 1,4,5-trisphosphate receptor (InsP3R) ligand binding domain splice variants (SI+/-/SII+). Receptor protein from COS-1 cells transfected with the type-1 InsP3R expression plasmids (pInsP3R-T1, pInsP3R-T1ALT) or control DNA were incorpo...

journal_title：Biophysical journal

authors： Ramos-Franco J,Caenepeel S,Fill M,Mignery G

更新日期：1998-12-01 00:00:00

abstract：:Back-of-the-envelope or rule-of-thumb calculations involving rough estimates of quantities play a central scientific role in developing intuition about the structure and behavior of physical systems, for example in so-called Fermi problems in the physical sciences. Such calculations can be used to powerfully and quant...

journal_title：Biophysical journal

authors： Johnston IG,Rickett BC,Jones NS

更新日期：2014-12-02 00:00:00

abstract：:Air dried films of purple membranes (PM) from Halobacterium halobium containing the photochromic protein bacteriorhodopsin (BR) were prepared and the BR-photocycle of this material analyzed. The absorption maxima of the initial state B (lambda(max) = 570 nm) and the photochemical intermediate M (lambda(max) = 412 nm),...

journal_title：Biophysical journal

authors： Hampp N,Bräuchle C,Oesterhelt D

更新日期：1990-07-01 00:00:00

abstract：:Tip links are thought to gate the mechanically sensitive transduction channels of hair cells, but how they form during development and regeneration remains mysterious. In particular, it is unclear how tip links are strung between stereocilia so that they are oriented parallel to a single axis; why their polarity is un...

journal_title：Biophysical journal

authors： Tompkins N,Spinelli KJ,Choi D,Barr-Gillespie PG

更新日期：2017-10-17 00:00:00

abstract：:Sickle hemoglobin forms long, multistranded polymers that account for the pathophysiology of the disease. The molecules in these polymers make significant contacts along the polymer axis (i.e., axial contacts) as well as making diagonally directed contacts (i.e., lateral contacts). The axial contacts do not engage the...

journal_title：Biophysical journal

authors： Wang Y,Ferrone FA

更新日期：2013-11-05 00:00:00

abstract：:Giant bilayer vesicles were reconstituted from several lipids and lipid/cholesterol (CHOL) mixtures: stearolyloleoylphosphatidylcholine (SOPC), bovine sphingomyelin (BSM), diarachidonylphosphatidylcholine (DAPC), SOPC/CHOL, BSM/CHOL, DAPC/CHOL, and extracted red blood cell (RBC) lipids with native cholesterol. Single-...

journal_title：Biophysical journal

authors： Needham D,Nunn RS

更新日期：1990-10-01 00:00:00

abstract：:The calcium release channels/ryanodine receptors (RyRs) usually form two-dimensional regular lattices in the endoplasmic/sarcoplasmic reticulum membranes. However, the function and modulation of the interaction between neighboring RyRs are still unknown. Here, with an in vitro aqueous system, we demonstrate that the i...

journal_title：Biophysical journal

authors： Hu XF,Liang X,Chen KY,Xie H,Xu Y,Zhu PH,Hu J

更新日期：2005-09-01 00:00:00

abstract：:To record post synaptic potentials or electrical activity from processes of single cells in a central nervous system (CNS) preparation in situ, voltage sensitive dyes can be injected intracellularly, thereby staining only the cell under investigation. We report the structure, evaluation, and synthesis of 11 fluorescen...

journal_title：Biophysical journal

authors： Grinvald A,Salzberg BM,Lev-Ram V,Hildesheim R

更新日期：1987-04-01 00:00:00

abstract：:The extracellular space of the brain is the heterogeneous porous medium formed by the spaces between the brain cells. Diffusion in this interstitial space is the mechanism by which glucose and oxygen are delivered to the brain cells from the vascular system. It is also a medium for the transport of certain information...

journal_title：Biophysical journal

authors： Nandigam RK,Kroll DM

更新日期：2007-05-15 00:00:00

abstract：:Members of the epidermal growth factor receptor family play important roles in various cellular processes, both in physiological and in pathological conditions. Dimerization and autophosphorylation of these receptor tyrosine kinases are key events of signal transduction. Details of the molecular events of the signalin...

journal_title：Biophysical journal

authors： Bagossi P,Horváth G,Vereb G,Szöllösi J,Tözsér J

更新日期：2005-02-01 00:00:00

abstract：:Apparent diffusion coefficient (ADC) measurement in the lung using gas magnetic resonance imaging is a promising technique with potential for reflecting changes in lung microstructure. Despite some recent impressive human applications, full interpretation of ADC measures remains an elusive goal, due to a lack of detai...

journal_title：Biophysical journal

authors： Pérez-Sánchez JM,Rodríguez I,Ruiz-Cabello J

更新日期：2009-07-22 00:00:00

abstract：:A new method of fluorescence microscopy for cell imaging has been developed that takes advantage of the spatial variations of fluorescence lifetimes in single cells as a source of image contrast, and thus it is named "fluorescence lifetime imaging microscopy (flimscopy)". Since time-resolved fluorescence measurements ...

journal_title：Biophysical journal

authors： Oida T,Sako Y,Kusumi A

更新日期：1993-03-01 00:00:00

abstract：:Deuterium NMR spectroscopy was used to study how the positioning of a dimerization motif within a transbilayer polypeptide influences its orientation and dynamics in bilayers. Three polypeptide variants comprising glycophorin A transmembrane (GpATM) dimerization motifs incorporated into lysine-terminated poly-leucine-...

journal_title：Biophysical journal

authors： McDonald MC,Booth V,Morrow MR

更新日期：2011-02-02 00:00:00

abstract：:This work describes the first cell-based model of tumor-induced angiogenesis. At the extracellular level, the model describes diffusion, uptake, and decay of tumor-secreted pro-angiogenic factor. At the cellular level, the model uses the cellular Potts model based on system-energy reduction to describe endothelial cel...

journal_title：Biophysical journal

authors： Bauer AL,Jackson TL,Jiang Y

更新日期：2007-05-01 00:00:00

abstract：:In the plant blue-light sensor phototropin, illumination of the chromophoric LOV domains causes activation of the serine/threonine kinase domain. Flavin mononucleotide (FMN) is a chromophore molecule in the two LOV domains (LOV1 and LOV2), but only LOV2 is responsible for kinase activation. Previous studies reported a...

journal_title：Biophysical journal

authors： Yamamoto A,Iwata T,Sato Y,Matsuoka D,Tokutomi S,Kandori H

更新日期：2009-04-08 00:00:00

abstract：:Although cell-cell interactions are known to significantly affect the kinetics of intracellular ice formation (IIF) during tissue freezing, this effect is not well understood. Progress in elucidating the mechanism and role of intercellular ice propagation in tissue freezing has been hampered in part by limitations in ...

journal_title：Biophysical journal

authors： Irimia D,Karlsson JO

更新日期：2005-01-01 00:00:00