The solution structure of the four-way DNA junction at low-salt conditions: a fluorescence resonance energy transfer analysis.

abstract：:We used high-resolution atomic force microscopy to image the compaction of linear and circular DNA by the yeast mitochondrial protein Abf2p, which plays a major role in packaging mitochondrial DNA. Atomic force microscopy images show that protein binding induces drastic bends in the DNA backbone for both linear and ci...

journal_title：Biophysical journal

authors： Friddle RW,Klare JE,Martin SS,Corzett M,Balhorn R,Baldwin EP,Baskin RJ,Noy A

更新日期：2004-03-01 00:00:00

abstract：:Myoplasmic free calcium transients were measured with antipyrylazo III in voltage clamped segments of frog skeletal muscle fibers and were used to calculate the rate of release (Rrel) of calcium from the sarcoplasmic reticulum. Intramembrane charge movement was measured for the same pulses in the same fibers. During a...

journal_title：Biophysical journal

authors： Simon BJ,Schneider MF

更新日期：1988-12-01 00:00:00

abstract：:The role of the extracellular Glu side chains of bacteriorhodopsin in the proton transport mechanism has been studied using the single mutants E9Q, E74Q, E194Q, and E204Q; the triple mutant E9Q/E194Q/E204Q; and the quadruple mutant E9Q/E74Q/E194Q/E204Q. Steady-state difference and deconvoluted Fourier transform infrar...

journal_title：Biophysical journal

authors： Lazarova T,Sanz C,Querol E,Padrós E

更新日期：2000-04-01 00:00:00

abstract：:The determination of intracellular protein concentrations is a prerequisite for understanding protein interaction networks in systems biology. Today, protein quantification is based either on mass spectrometry, which requires large cell numbers and sophisticated measurement protocols, or on quantitative Western blotti...

journal_title：Biophysical journal

authors： Chung YD,Sinzinger MD,Bovee-Geurts P,Krause M,Dinkla S,Joosten I,Koopman WJ,Adjobo-Hermans MJ,Brock R

更新日期：2011-12-07 00:00:00

abstract：:The effects of ultraviolet light (UV) irradiation on the rate of DNA replication in synchronized Chinese hamster ovary (CHO) cells were investigated. A technique for measuring semiconservative DNA replication was employed that involved growing the cells in medium containing 5-bromodeoxyuridine and subsequently determi...

journal_title：Biophysical journal

authors： Meyn RE,Hewitt RR,Thomas LF,Humphrey RM

更新日期：1976-05-01 00:00:00

abstract：:Biological membranes composed of lipids and proteins are in contact with electrolytes like aqueous NaCl solutions. Based on molecular dynamics studies it is widely believed that Na(+) ions specifically bind to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes, whereas Cl(-) ions stay in solution. Here,...

journal_title：Biophysical journal

authors： Knecht V,Klasczyk B

更新日期：2013-02-19 00:00:00

abstract：:We studied the cold unfolding of myoglobin with Fourier transform infrared spectroscopy and compared it with pressure and heat unfolding. Because protein aggregation is a phenomenon with medical as well as biotechnological implications, we were interested in both the structural changes as well as the aggregation behav...

journal_title：Biophysical journal

authors： Meersman F,Smeller L,Heremans K

更新日期：2002-05-01 00:00:00

abstract：:Experimental studies have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high repetition rate lasers, a phenomenon we call "light quenching." We now describe the theory of light quenching and some of its effects on the steady-state and time-resolved intensity and ani...

journal_title：Biophysical journal

authors： Kuśba J,Bogdanov V,Gryczynski I,Lakowicz JR

更新日期：1994-11-01 00:00:00

abstract：:Previously, we discussed the calculation of the dipole moments of small proteins using the three-dimensional protein data-base. Our results demonstrate that the calculated dipole moments are in acceptable agreement with measured values. We, however, noted the difficulty of the calculation with larger proteins, in part...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1993-05-01 00:00:00

abstract：:We simulate a simple phenomenological model describing phase behavior in a multicomponent membrane, a model capable of producing macroscopic phase separation, modulated phases, and microemulsions, all of which have been discussed in terms of raft phenomena. We show that one effect of thermal fluctuations on the mean-f...

journal_title：Biophysical journal

authors： Shlomovitz R,Maibaum L,Schick M

更新日期：2014-05-06 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation with fluorescence detection has emerged as a powerful method for the study of interacting systems of macromolecules. It combines picomolar sensitivity with high hydrodynamic resolution, and can be carried out with photoswitchable fluorophores for multicomponent dis...

journal_title：Biophysical journal

authors： Chaturvedi SK,Zhao H,Schuck P

更新日期：2017-04-11 00:00:00

abstract：:Kv1 potassium channels are widely distributed in mammalian tissues and are involved in a variety of functions from controlling the firing rate of neurons to maturation of T-lymphocytes. Here we show that the newly described KCNE4 beta-subunit has a drastic inhibitory effect on currents generated by Kv1.1 and Kv1.3 pot...

journal_title：Biophysical journal

authors： Grunnet M,Rasmussen HB,Hay-Schmidt A,Rosenstierne M,Klaerke DA,Olesen SP,Jespersen T

更新日期：2003-09-01 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:Using a method they developed, Stamatoff and Krimm (1976) have phased swelling data from nerve myelin. Although most phases agree with those I determined previously, there are a few differences. In this letter the two different phasings, theirs and my own, are used to compute the corresponding electron-density profile...

journal_title：Biophysical journal

authors： Blaurock AE

更新日期：1979-04-01 00:00:00

abstract：:We combine ensemble and single-molecule spectroscopy to gain insight into the energy transfer between chlorophylls (Chls) in peridinin-chlorophyll-protein (PCP) complexes reconstituted with Chl a, Chl b, as well as both Chl a and Chl b. The main focus is the heterochlorophyllous system (Chl a/b-N-PCP), and reference i...

journal_title：Biophysical journal

authors： Mackowski S,Wörmke S,Brotosudarmo TH,Jung C,Hiller RG,Scheer H,Bräuchle C

更新日期：2007-11-01 00:00:00

abstract：:The GroE chaperonin system facilitates protein folding in an ATP-dependent manner. It has remained unclear why some proteins are obligate clients of the GroE system, whereas other closely related proteins are able to fold efficiently in its absence. Factors that cause folding to be slower affect kinetic partitioning b...

journal_title：Biophysical journal

authors： Bandyopadhyay B,Mondal T,Unger R,Horovitz A

更新日期：2019-01-08 00:00:00

abstract：:We have obtained new insights into the behavior of a class of excitable systems when a stimulus, or parameter, is slowly tuned through a threshold value. Such systems do not accommodate no matter how slowly a stimulus ramp is applied, and the stimulus value at onset of repetitive activity shows a curious, nonmonotonic...

journal_title：Biophysical journal

authors： Rinzel J,Baer SM

更新日期：1988-09-01 00:00:00

abstract：:In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exh...

journal_title：Biophysical journal

authors： Linari M,Piazzesi G,Pertici I,Dantzig JA,Goldman YE,Lombardi V

更新日期：2020-03-10 00:00:00

abstract：:Here we extend the ability to predict hydrodynamic coefficients and other solution properties of rigid macromolecular structures from atomic-level structures, implemented in the computer program HYDROPRO, to models with lower, residue-level resolution. Whereas in the former case there is one bead per nonhydrogen atom,...

journal_title：Biophysical journal

authors： Ortega A,Amorós D,García de la Torre J

更新日期：2011-08-17 00:00:00

abstract：:Whether proteins or lipids are the primary target sites for general anesthetic action has engendered considerable debate. Recent in vivo studies have shown that the S(+) and R(-) enantiomers of isoflurane are not equipotent, implying involvement of proteins. Bovine serum albumin (BSA), a soluble protein devoid of lipi...

journal_title：Biophysical journal

authors： Xu Y,Tang P,Firestone L,Zhang TT

更新日期：1996-01-01 00:00:00

abstract：:Structure-function studies of the Cys loop family of ionotropic neurotransmitter receptors (GABA, nACh, 5-HT(3), and glycine receptors) have resulted in a six-loop (A-F) model of the agonist-binding site. Key amino acids have been identified in these loops that associate with, and stabilize, bound ligand. The next ste...

journal_title：Biophysical journal

authors： Khatri A,Sedelnikova A,Weiss DS

更新日期：2009-01-01 00:00:00

abstract：:Translation is a central cellular process and the complexity of its mechanism necessitates mathematical frameworks to better understand system properties and make quantitative predictions. We have developed a gene sequence-specific mechanistic model for translation which accounts for all the elementary steps of transl...

journal_title：Biophysical journal

authors： Zouridis H,Hatzimanikatis V

更新日期：2008-08-01 00:00:00

abstract：:We have monitored the organization and dynamics of the hemolytic peptide melittin in membranes containing cholesterol by utilizing the intrinsic fluorescence properties of its functionally important sole tryptophan residue and circular dichroism spectroscopy. The significance of this study is based on the fact that th...

journal_title：Biophysical journal

authors： Raghuraman H,Chattopadhyay A

更新日期：2004-10-01 00:00:00

abstract：:Interactions of membrane anchored molecules such as glycolipids with a membrane surface are important in determining headgroup conformation. It is therefore essential to represent these membrane surface interactions in molecular modeling studies of glycolipids and other membrane bound molecules. We introduce here an e...

journal_title：Biophysical journal

authors： Ram P,Kim E,Thomson DS,Howard KP,Prestegard JH

更新日期：1992-12-01 00:00:00

abstract：:We present a new method for analyzing the dynamics of conformational fluctuations of individual flexible polymer molecules. In single-particle tracking (SPT), one end of the polymer molecule is tethered to an immobile substratum. A microsphere attached to the other end serves as an optical marker. The conformational f...

journal_title：Biophysical journal

authors： Qian H,Elson EL

更新日期：1999-03-01 00:00:00

abstract：:It has been a significant challenge to quantitatively study the dynamic intracellular processes in live cells. These studies are essential for a thorough understanding of the underlying mechanisms regulating the signaling pathways and the transitions between cell cycle stages. Our studies of Cdc20, an important mitoti...

journal_title：Biophysical journal

authors： Wang Z,Shah JV,Berns MW,Cleveland DW

更新日期：2006-07-01 00:00:00

abstract：:A filopodium is a cytoplasmic projection, exquisitely built and regulated, which extends from the leading edge of the migrating cell, exploring the cell's neighborhood. Commonly, filopodia grow and retract after their initiation, exhibiting rich dynamical behaviors. We model the growth of a filopodium based on a stoch...

journal_title：Biophysical journal

authors： Lan Y,Papoian GA

更新日期：2008-05-15 00:00:00

abstract：:Reattachment and healing of tendon to bone poses a persistent clinical challenge and often results in poor outcomes, in part because the mechanisms that imbue the uninjured tendon-to-bone attachment with toughness are not known. One feature of typical tendon-to-bone surgical repairs is direct attachment of tendon to s...

journal_title：Biophysical journal

authors： Hu Y,Birman V,Deymier-Black A,Schwartz AG,Thomopoulos S,Genin GM

更新日期：2015-01-20 00:00:00

abstract：:Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

journal_title：Biophysical journal

authors： Koeppe RE 2nd,Schoenborn BP

更新日期：1984-03-01 00:00:00

abstract：:The single photon response in vertebrate phototransduction is highly reproducible despite a number of random components of the activation cascade, including the random activation site, the random walk of an activated receptor, and its quenching in a random number of steps. Here we use a previously generated and tested...

journal_title：Biophysical journal

authors： Bisegna P,Caruso G,Andreucci D,Shen L,Gurevich VV,Hamm HE,DiBenedetto E

更新日期：2008-05-01 00:00:00