Quantitative study of polymer conformation and dynamics by single-particle tracking.

abstract：:Molecular interactions are often transient and might change within the window of observation, leading to changes in molecule movement. Therefore, accurate motion analysis often requires transient motion classification. Here we present an accurate and computationally efficient transient mobility analysis framework, ter...

journal_title：Biophysical journal

authors： Vega AR,Freeman SA,Grinstein S,Jaqaman K

更新日期：2018-03-13 00:00:00

abstract：:We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments sh...

journal_title：Biophysical journal

authors： Hsueh YW,Weng CJ,Chen MT,Thewalt J,Zuckermann M

更新日期：2010-04-07 00:00:00

abstract：:The lateral distribution of N-[10(1-pyrenyl)decanoyl]-sphingomyelin (PyrSPM) and N-[10(1-pyrenyl)decanoyl]-glucocerebroside (PyrGlcCer) was studied in multilamellar vesicles of 1,2-dipalmitoyl-, 1,2-dimyristoyl-, and 1-palmitoyl-2-oleoyl-phosphatidylcholine (DPPC, DMPC, and POPC, respectively) under anaerobic conditio...

journal_title：Biophysical journal

authors： Hresko RC,Sugár IP,Barenholz Y,Thompson TE

更新日期：1987-05-01 00:00:00

abstract：:Alterations in bone tissue composition during osteoporosis likely disrupt the mechanical environment of bone cells and may thereby initiate a mechanobiological response. It has proved challenging to characterize the mechanical environment of bone cells in vivo, and the mechanical environment of osteoporotic bone cells...

journal_title：Biophysical journal

authors： Verbruggen SW,Mc Garrigle MJ,Haugh MG,Voisin MC,McNamara LM

更新日期：2015-04-07 00:00:00

abstract：:The molecular weight of single-stranded DNA from the slime mold Physarum polycephalum has been determined by alkaline gradient centrifugation. The average molecular weight during DNA synthesis ( approximately 1.5 x 10(7) D) is less than that observed in nonsynthetic periods ( approximately 4 x 10(7) D). On the basis o...

journal_title：Biophysical journal

authors： McGrath RA,Williams RW

更新日期：1967-05-01 00:00:00

abstract：:The development of strategies to measure plasma membrane osmotic water permeability (Pf) in epithelial cells has been motivated by the identification of a family of molecular water channels. A general approach utilizing interferometry to measure cell shape and volume was developed and applied to measure Pf in cell lay...

journal_title：Biophysical journal

authors： Farinas J,Verkman AS

更新日期：1996-12-01 00:00:00

abstract：:The information theory measure of transfer entropy is used to extract the causality of correlated motions from molecular dynamics simulations. For each pair of correlated residues, the method quantifies which residue drives the correlated motions, and which residue responds. The measure reveals how correlated motions ...

journal_title：Biophysical journal

authors： Kamberaj H,van der Vaart A

更新日期：2009-09-16 00:00:00

abstract：:Cationic liposomes (CLs) are used worldwide as gene vectors (carriers) in nonviral clinical applications of gene delivery, albeit with unacceptably low transfection efficiencies (TE). We present three-dimensional laser scanning confocal microscopy studies revealing distinct interactions between CL-DNA complexes, for b...

journal_title：Biophysical journal

authors： Lin AJ,Slack NL,Ahmad A,George CX,Samuel CE,Safinya CR

更新日期：2003-05-01 00:00:00

abstract：:We examined the effects of brief current pulses on the pacemaker oscillations of the Purkinje fiber using the model of McAllister , Noble, and Tsien (1975. J. Physiol. [Lond.]. 251:1-57). This model was used to construct phase-response curves for brief electric stimuli to find "black holes," where rhythmic activity of...

journal_title：Biophysical journal

authors： Chay TR,Lee YS

更新日期：1984-04-01 00:00:00

abstract：:The wormlike-chain (WLC) model is widely used to describe the energetics of DNA bending. Motivated by recent experiments, alternative, so-called subelastic chain models were proposed that predict a lower elastic energy of highly bent DNA conformations. Until now, no unambiguous verification of these models has been ob...

journal_title：Biophysical journal

authors： Schöpflin R,Brutzer H,Müller O,Seidel R,Wedemann G

更新日期：2012-07-18 00:00:00

abstract：:We use molecular dynamics simulations to characterize the influence of cholesterol (Chol) on the interaction between the anticancer drug doxorubicin (DOX) and a dipalmitoyl phosphatidylcholine/Chol lipid bilayer. We calculate the potential of mean force, which gives us an estimate of the free energy barrier for DOX tr...

journal_title：Biophysical journal

authors： Yacoub TJ,Reddy AS,Szleifer I

更新日期：2011-07-20 00:00:00

abstract：:Finite difference solutions of the Poisson-Boltzmann equation are used to calculate the pKa values of the functionally important ionizable groups in bacteriorhodopsin. There are strong charge-charge interactions between the residues in the binding site leading to the possibility of complex titration behavior. Structur...

journal_title：Biophysical journal

authors： Sampogna RV,Honig B

更新日期：1994-05-01 00:00:00

abstract：:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Prot...

journal_title：Biophysical journal

authors： van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

更新日期：2013-03-05 00:00:00

abstract：:The endoplasmic reticulum (ER) in plant cells forms a highly dynamic network of complex geometry. ER network morphology and dynamics are influenced by a number of biophysical processes, including filament/tubule tension, viscous forces, Brownian diffusion, and interactions with many other organelles and cytoskeletal e...

journal_title：Biophysical journal

authors： Lin C,White RR,Sparkes I,Ashwin P

更新日期：2017-07-11 00:00:00

abstract：:Giant vesicles formed of 1,2-dipalmitoylphosphatidylcholine (DPPC) and sterols (cholesterol or ergosterol) in water and water/ethanol solutions have been used to examine the effect of sterol composition and ethanol concentration on the area compressibility modulus (K(a)), overall mechanical behavior, vesicle morpholog...

journal_title：Biophysical journal

authors： Tierney KJ,Block DE,Longo ML

更新日期：2005-10-01 00:00:00

abstract：:Kinesin and cytoplasmic dynein are microtubule-based motor proteins that actively transport material throughout the cell. Microtubules can intersect at a variety of angles both near the nucleus and at the cell periphery, and the behavior of molecular motors at these intersections has implications for long-range transp...

journal_title：Biophysical journal

authors： Ross JL,Shuman H,Holzbaur EL,Goldman YE

更新日期：2008-04-15 00:00:00

abstract：:Two mechanisms have been proposed to account for solute permeation of lipid bilayers. Partitioning into the hydrophobic phase of the bilayer, followed by diffusion, is accepted by many for the permeation of water and other small neutral solutes, but transient pores have also been proposed to account for both water and...

journal_title：Biophysical journal

authors： Paula S,Volkov AG,Van Hoek AN,Haines TH,Deamer DW

更新日期：1996-01-01 00:00:00

abstract：:Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments a...

journal_title：Biophysical journal

authors： Mao B,Ebrey TG,Crouch R

更新日期：1980-02-01 00:00:00

abstract：:The kinetics of methyl-, ethyl-, iso-propyl-, and ter-butyl-isocyanide binding to Aplysia limacina myoglobin (distal His----Lys) and the isolated beta chains from hemoglobin Zurich (distal His----Arg) have been investigated by flash photolysis at various temperatures above 0 degrees C. Sperm whale (Physter catodon) my...

journal_title：Biophysical journal

authors： Di Iorio EE,Winterhalter KH,Giacometti GM

更新日期：1987-03-01 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+ ATPase was derivatized with 4,4'-diisothiocyanatostilbene-2,2'-sulfonic acid (DIDS), and complete enzyme inactivation was produced with a molecular stoichiometry of one DIDS per ATPase. It was determined by peptide analysis and sequencing that Lys492 and Lys515 were the ATPase residues...

journal_title：Biophysical journal

authors： Hua S,Inesi G

更新日期：1997-10-01 00:00:00

abstract：:Cholesterol is essential to the formation of phase-separated lipid domains in membranes. Lipid domains can exist in different thermodynamic phases depending on the molecular composition and play significant roles in determining structure and function of membrane proteins. We investigate the role of cholesterol in the ...

journal_title：Biophysical journal

authors： Pantelopulos GA,Straub JE

更新日期：2018-12-04 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:Pure Michaelis-Menten enzymes have been studied (i.e., enzymes with a hyperbolic (S, V) behavior in a well-stirred solution). When such enzymes are associated with a structure in vitro, even in the simplest conceivable form (immobilization in a homogeneous gel), they can produce enzymic or transport reactions with man...

journal_title：Biophysical journal

authors： Vincent JC,Thellier M

更新日期：1983-01-01 00:00:00

abstract：:For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating ...

journal_title：Biophysical journal

authors： Hustedt EJ,Smirnov AI,Laub CF,Cobb CE,Beth AH

更新日期：1997-04-01 00:00:00

abstract：:Membrane fusion proceeds via formation of intermediate nonbilayer structures. The stalk model of fusion intermediate is commonly recognized to account for the major phenomenology of the fusion process. However, in its current form, the stalk model poses a challenge. On one hand, it is able to describe qualitatively th...

journal_title：Biophysical journal

authors： Kozlovsky Y,Kozlov MM

更新日期：2002-02-01 00:00:00

abstract：:In diseases of the brain, the distribution and properties of ion channels display deviations from healthy control subjects. We studied three cases of ion channel alteration related to epileptogenesis. The first case of ion channel alteration represents an enhanced sodium current, the second case addresses the downregu...

journal_title：Biophysical journal

authors： Tigerholm J,Fransén E

更新日期：2011-10-19 00:00:00

abstract：:Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golg...

journal_title：Biophysical journal

authors： Li J,Barylko B,Eichorst JP,Mueller JD,Albanesi JP,Chen Y

更新日期：2016-08-09 00:00:00

abstract：:Difference spectra between resting and excited nerve in the infrared region between 2000 and 1000 cm(-1) have been examined with a resolution of 0.5 cm(-1). Spectra were obtained with a modified Perkin-Elmer model 521 grating infrared spectrophotometer (Perkin-Elmer Corp., Instrument Div., Norwalk, Conn.), and the sig...

journal_title：Biophysical journal

authors： Sherebrin MH,MacClement BA,Franko AJ

更新日期：1972-08-01 00:00:00

abstract：:This paper describes a framework model for proton conduction through gramicidin; a model designed to incorporate information from molecular dynamics and use this to predict conductance properties. The state diagram describes both motion of an excess proton within the pore as well as the reorientation of waters within ...

journal_title：Biophysical journal

authors： Schumaker MF,Pomès R,Roux B

更新日期：2001-01-01 00:00:00

abstract：:Antibodies are key tools in biomedical research and medicine. Their binding properties are classically measured in solution and characterized by an affinity. However, in physiological conditions, antibodies can bridge an immune effector cell and an antigen-presenting cell, implying that mechanical forces may apply to ...

journal_title：Biophysical journal

authors： González C,Chames P,Kerfelec B,Baty D,Robert P,Limozin L

更新日期：2019-04-23 00:00:00